Ciita (NM_001243760) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	C2t; C2ta; EG669998; Gm9475; Mhc2ta
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229417 representing NM_001243760 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCACTTCCAGGCCATCCTGGCCCAAGTACAGACACTGCTCTCCAGCCAGAAGCCCAGGCAGGTGC GGGCCCTCCTGGATGGCCTGCTGGAAGAAGAGCTGCTCTCACGGGAATACCACTGTGCCTTGCTGCATGA GCCTGATGGTGATGCCCTGGCCCGGAAGATTTCCCTGACCCTGCTGGAGAAAGGGGACTTAGACTTGACT TTCTTGAGCTGGGTCTGCAACAGTCTGCAGGCTCCCACGGTAGAGAGGGGCACCAGCTACAGGGACCATG GAGACCATAGTCTGTGTGCCACCATGGATCTGGGATCTCCAGAGGGCAGCTACCTGGAACTCCTTAACAG TGATGCCGACCCCCTACATCTCTACCACCTCTATGACCAGATGGACCTGGCTGGGGAGGAGGAGATCGAA CTCAGCTCAGAGCCAGACACAGATACCATCAACTGCGACCAGTTCAGCAAGCTGTTGCAGGACATGGAAC TGGATGAAGAGACCCGGGAGGCCTATGCCAACATTGCGGAACTGGATCAGTACGTGTTCCAGGATACCCA GCTCGAGGGCCTGAGCAAGGACCTCTTCAAGCACATTGGAGCAGAGGAAGGCTTTGGTGAGAACATAGAG ATCCCTGTAGAAGCAGGACAGAAGCCTCAGAAGAGACGCTTCCCGGAAGAGCATGCTATGGACTCAAAGC ACAGGAAGCTAGTGCCCACCTCTAGGACCTCACTGAACTATTTGGATCTCCCCACTGGGCACATCCAGAT CTTCACCACTCTGCCCCAGGGACTCTGGCAAATCTCAGGGGCTGGCACAGGTCTCTCCAGTGTCCTAATC TACCACGGTGAGATGCCCCAGGTCAACCAAGTGCTCCCTTCAAGCAGCCTCAGTATCCCCAGTCTCCCCG AGTCCCCAGACCGGCCTGGCTCCACCAGCCCCTTCACACCATCTGCAGCTGACCTGCCCAGCATGCCCGA ACCTGCGCTGACCTCCCGTGTAAATGAGACAGAGGACACATCTCCCTCCCCATGCCAAGAGGGTCCCGAG TCTTCCATCAAGCTTCCAAAATGGCCAGAGGCTGTGGAGCGATTCCAGCACTCCCTACAGGACAAATACA AGGCATTGCCCCAGAGCCCAAGGGGTCCTCTGGTGGCCGTGGAGCTGGTACGGGCCAGGCTGGAAAGAGG CAGCAACAAGAGCCAGGAAAGGGAGCTGGCCACTCCCGACTGGACAGAGCGCCAGCTAGCCCACGGTGGT CTGGCAGAGGTACTTCAGGTTGTCAGTGACTGCAGGCGACCAGGAGAGACACAGGTGGTCGCTGTGCTGG GCAAGGCTGGCCAGGGAAAGAGCCACTGGGCCAGGACAGTGAGTCACACCTGGGCATGTGGCCAGTTGCT ACAATATGACTTTGTCTTCTATGTCCCCTGTCATTGCTTGGATCGTCCCGGGGACACCTACCACCTGCGG GATCTGCTCTGTCCCCCGAGCCTGCAGCCACTGGCCATGGATGACGAGGTCCTTGATTATATCGTGAGGC AGCCAGACCGTGTTCTGCTCATCCTAGATGCTTTCGAGGAGCTAGAGGCCCAAGATGGCCTCCTGCACGG ACCCTGTGGATCTCTGTCCCCAGAGCCCTGCTCCCTCCGAGGACTGCTGGCTGGGATCTTCCAGCGGAAG CTACTGCGAGGCTGCACACTGCTCCTCACAGCCCGGCCCCGGGGCCGCCTGGCTCAGAGCCTGAGCAAGG CAGATGCCATCTTTGAGGTGCCCAGCTTCTCTACCAAGCAGGCCAAGACTTACATGAGGCACTACTTTGA GAACTCAGGGACAGCGGGGAACCAAGACAAGGCCCTGGGCCTCCTGGAGGGCCAGCCTCTTCTCTGCAGC TATAGTCACAGCCCTGTTGTGTGCAGGGCTGTGTGCCAGCTCTCCAAGGCCCTGCTAGAACAGGGCACAG AGGCCCAGCTACCTTGTACACTTACAGGACTCTATGTCAGCCTGCTAGGTCCTGCAGCTCAGAACAGTCC TCCCGGAGCCTTAGTCGAGCTGGCCAAGCTGGCCTGGGAGCTGGGACGAAGACACCAAAGCACCTTGCAA GAAACCCGGTTTTCATCCGTGGAGGTGAAAACCTGGGCAGTGACCCAAGGCTTGATGCAGCAGACCCTGG AGACCACGGAGGCTCAACTGGCCTTCTCCAGTTTTCTGCTACAGTGTTTCCTGGGTGCTGTGTGGCTGGC ACAGTGCAATGAAATCAAAGACAAGGAGCTGCCACAGTACCTGGCCTTGACTCCGAGGAAGAAGAGACCC TATGACAACTGGCTGGAGGGTGTACCACGCTTTCTGGCTGGATTAGTTTTCCAGCCTCGAGCCCACTGCC TGGGAGCTCTGGTGGAGCCTGCAGTGGCTGCAGTGGCGGATAGGAAACAGAAGGTTCTTACCAGGTACCT GAAGCGCCTGAAGCTGGGGACACTCCGGGCAGGGAGGCTGCTGGAGCTGCTCCACTGTGCCCACGAGACA CAGCAACCTGGGATATGGGAGCATGTTGCACACCAGCTCCCTGGCCACCTCTCCTTCCTGGGCACCCGGC TCACACCCCCAGATGTGTATGTGCTGGGCAGGGCCTTGGAGACAGCCAGCCAGGACTTCTCCTTGGACCT TCGTCAGACTGGCGTTGAGCCTTCTGGACTGGGAAACCTCGTGGGACTCAGCTGTGTCACCAGTTTCAGG GCCTCCTTGAGTGATACAATGGCATTATGGGAGTCCCTTCAGCAGCAGGGAGAAGCCCAGCTACTCCAGG CGGCAGAGGAGAAGTTCACCATTGAGCCATTTAAAGCCAAATCCCCAAAGGATGTGGAAGACCTGGATCG TCTCGTGCAGACCCAGAGGCTGAGAAACCCCTCAGAAGATGCAGCCAAGGATCTTCCTGCCATCCGGGAC CTTAAGAAGCTAGAGTTTGCGTTGGGCCCCATCTTGGGCCCCCAGGCTTTCCCCACACTGGCAAAGATCC TTCCAGCCTTCTCTTCTCTGCAACACCTGGACCTGGACTCACTTAGTGAGAACAAGATCGGAGACAAGGG TGTGTCGAAGCTCTCAGCCACCTTCCCTCAGCTGAAGGCCCTGGAGACGCTCAACTTGTCCCAAAACAAC ATCACTGATGTGGGTGCCTGCAAGCTTGCAGAAGCTCTGCCAGCCCTAGCCAAGTCCCTCCTAAGGCTGA GCTTGTACAATAACTGCATCTGTGACAAAGGAGCCAAGAGCCTGGCACAAGTACTTCCGGACATGGTGTC CCTGCGTGTGATGGATGTCCAGTTCAACAAGTTCACGGCTGCCGGTGCCCAGCAACTGGCCTCCAGCCTT CAGAAGTGCCCTCAGGTGGAAACACTGGCAATGTGGACACCCACTATCCCCTTTGGGGTTCAGGAACACC TGCAGCAGCTGGATGCCAGGATCAGTCTGAGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001243760
Insert Size	3465 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001243760.2, NP_001230689.1
RefSeq Size	5435 bp
RefSeq ORF	3465 bp
Locus ID	12265
Gene Summary	This gene encodes a member of the NOD-like receptor protein family. This protein acts as a transcriptional coactivator and component of the enhanceosome complex to stimulate transcription of MHC class II genes in the adaptive immune response. This protein may also regulate the transcription of MHC class I genes. Mutations in the human gene have been linked to a rare immunodeficiency, bare lymphocyte syndrome, and homozygous knockout mice exhibit many features of this disease. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014] Transcript Variant: This variant (1) uses an alternate in-frame splice site in the 5' coding region, compared to variant 4. It encodes isoform 1, which is shorter than isoform 4.

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