Ciita (NM_001243761) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	C2t; C2ta; EG669998; Gm9475; Mhc2ta
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229343 representing NM_001243761 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGCAGCACTCAGAAGCACGGGGCACAGCCACAGCCGCGACCATAGTCTGTGTGCCACCATGGATC TGGGATCTCCAGAGGGCAGCTACCTGGAACTCCTTAACAGTGATGCCGACCCCCTACATCTCTACCACCT CTATGACCAGATGGACCTGGCTGGGGAGGAGGAGATCGAACTCAGCTCAGAGCCAGACACAGATACCATC AACTGCGACCAGTTCAGCAAGCTGTTGCAGGACATGGAACTGGATGAAGAGACCCGGGAGGCCTATGCCA ACATTGCGGAACTGGATCAGTACGTGTTCCAGGATACCCAGCTCGAGGGCCTGAGCAAGGACCTCTTCAT AGAGCACATTGGAGCAGAGGAAGGCTTTGGTGAGAACATAGAGATCCCTGTAGAAGCAGGACAGAAGCCT CAGAAGAGACGCTTCCCGGAAGAGCATGCTATGGACTCAAAGCACAGGAAGCTAGTGCCCACCTCTAGGA CCTCACTGAACTATTTGGATCTCCCCACTGGGCACATCCAGATCTTCACCACTCTGCCCCAGGGACTCTG GCAAATCTCAGGGGCTGGCACAGGTCTCTCCAGTGTCCTAATCTACCACGGTGAGATGCCCCAGGTCAAC CAAGTGCTCCCTTCAAGCAGCCTCAGTATCCCCAGTCTCCCCGAGTCCCCAGACCGGCCTGGCTCCACCA GCCCCTTCACACCATCTGCAGCTGACCTGCCCAGCATGCCCGAACCTGCGCTGACCTCCCGTGTAAATGA GACAGAGGACACATCTCCCTCCCCATGCCAAGAGGGTCCCGAGTCTTCCATCAAGCTTCCAAAATGGCCA GAGGCTGTGGAGCGATTCCAGCACTCCCTACAGGACAAATACAAGGCATTGCCCCAGAGCCCAAGGGGTC CTCTGGTGGCCGTGGAGCTGGTACGGGCCAGGCTGGAAAGAGGCAGCAACAAGAGCCAGGAAAGGGAGCT GGCCACTCCCGACTGGACAGAGCGCCAGCTAGCCCACGGTGGTCTGGCAGAGGTACTTCAGGTTGTCAGT GACTGCAGGCGACCAGGAGAGACACAGGTGGTCGCTGTGCTGGGCAAGGCTGGCCAGGGAAAGAGCCACT GGGCCAGGACAGTGAGTCACACCTGGGCATGTGGCCAGTTGCTACAATATGACTTTGTCTTCTATGTCCC CTGTCATTGCTTGGATCGTCCCGGGGACACCTACCACCTGCGGGATCTGCTCTGTCCCCCGAGCCTGCAG CCACTGGCCATGGATGACGAGGTCCTTGATTATATCGTGAGGCAGCCAGACCGTGTTCTGCTCATCCTAG ATGCTTTCGAGGAGCTAGAGGCCCAAGATGGCCTCCTGCACGGACCCTGTGGATCTCTGTCCCCAGAGCC CTGCTCCCTCCGAGGACTGCTGGCTGGGATCTTCCAGCGGAAGCTACTGCGAGGCTGCACACTGCTCCTC ACAGCCCGGCCCCGGGGCCGCCTGGCTCAGAGCCTGAGCAAGGCAGATGCCATCTTTGAGGTGCCCAGCT TCTCTACCAAGCAGGCCAAGACTTACATGAGGCACTACTTTGAGAACTCAGGGACAGCGGGGAACCAAGA CAAGGCCCTGGGCCTCCTGGAGGGCCAGCCTCTTCTCTGCAGCTATAGTCACAGCCCTGTTGTGTGCAGG GCTGTGTGCCAGCTCTCCAAGGCCCTGCTAGAACAGGGCACAGAGGCCCAGCTACCTTGTACACTTACAG GACTCTATGTCAGCCTGCTAGGTCCTGCAGCTCAGAACAGTCCTCCCGGAGCCTTAGTCGAGCTGGCCAA GCTGGCCTGGGAGCTGGGACGAAGACACCAAAGCACCTTGCAAGAAACCCGGTTTTCATCCGTGGAGGTG AAAACCTGGGCAGTGACCCAAGGCTTGATGCAGCAGACCCTGGAGACCACGGAGGCTCAACTGGCCTTCT CCAGTTTTCTGCTACAGTGTTTCCTGGGTGCTGTGTGGCTGGCACAGTGCAATGAAATCAAAGACAAGGA GCTGCCACAGTACCTGGCCTTGACTCCGAGGAAGAAGAGACCCTATGACAACTGGCTGGAGGGTGTACCA CGCTTTCTGGCTGGATTAGTTTTCCAGCCTCGAGCCCACTGCCTGGGAGCTCTGGTGGAGCCTGCAGTGG CTGCAGTGGCGGATAGGAAACAGAAGGTTCTTACCAGGTACCTGAAGCGCCTGAAGCTGGGGACACTCCG GGCAGGGAGGCTGCTGGAGCTGCTCCACTGTGCCCACGAGACACAGCAACCTGGGATATGGGAGCATGTT GCACACCAGCTCCCTGGCCACCTCTCCTTCCTGGGCACCCGGCTCACACCCCCAGATGTGTATGTGCTGG GCAGGGCCTTGGAGACAGCCAGCCAGGACTTCTCCTTGGACCTTCGTCAGACTGGCGTTGAGCCTTCTGG ACTGGGAAACCTCGTGGGACTCAGCTGTGTCACCAGTTTCAGGGCCTCCTTGAGTGATACAATGGCATTA TGGGAGTCCCTTCAGCAGCAGGGAGAAGCCCAGCTACTCCAGGCGGCAGAGGAGAAGTTCACCATTGAGC CATTTAAAGCCAAATCCCCAAAGGATGTGGAAGACCTGGATCGTCTCGTGCAGACCCAGAGGCTGAGAAA CCCCTCAGAAGATGCAGCCAAGGATCTTCCTGCCATCCGGGACCTTAAGAAGCTAGAGTTTGCGTTGGGC CCCATCTTGGGCCCCCAGGCTTTCCCCACACTGGCAAAGATCCTTCCAGCCTTCTCTTCTCTGCAACACC TGGACCTGGACTCACTTAGTGAGAACAAGATCGGAGACAAGGGTGTGTCGAAGCTCTCAGCCACCTTCCC TCAGCTGAAGGCCCTGGAGACGCTCAACTTGTCCCAAAACAACATCACTGATGTGGGTGCCTGCAAGCTT GCAGAAGCTCTGCCAGCCCTAGCCAAGTCCCTCCTAAGGCTGAGCTTGTACAATAACTGCATCTGTGACA AAGGAGCCAAGAGCCTGGCACAAGTACTTCCGGACATGGTGTCCCTGCGTGTGATGGATGTCCAGTTCAA CAAGTTCACGGCTGCCGGTGCCCAGCAACTGGCCTCCAGCCTTCAGAAGTGCCCTCAGGTGGAAACACTG GCAATGTGGACACCCACTATCCCCTTTGGGGTTCAGGAACACCTGCAGCAGCTGGATGCCAGGATCAGTC TGAGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001243761
Insert Size	3228 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001243761.2, NP_001230690.1
RefSeq Size	5167 bp
RefSeq ORF	3228 bp
Locus ID	12265
UniProt ID	P79621
Gene Summary	This gene encodes a member of the NOD-like receptor protein family. This protein acts as a transcriptional coactivator and component of the enhanceosome complex to stimulate transcription of MHC class II genes in the adaptive immune response. This protein may also regulate the transcription of MHC class I genes. Mutations in the human gene have been linked to a rare immunodeficiency, bare lymphocyte syndrome, and homozygous knockout mice exhibit many features of this disease. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014] Transcript Variant: This variant (3) differs in the 5' UTR and uses an alternate start codon compared to variant 4. It encodes isoform 3, which is shorter and has a distinct N-terminus, compared to isoform 4.

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