Slc4a4 (NM_001197147) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI835705; NBC; NBC1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229334 representing NM_001197147 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGATGAAGCTGTCCTGGACAGAGGGGCTTCCTTCCTTAAACATGTGTGTGATGAAGAAGAAGTAG AAGGTCACCACACGATCTACATTGGGGTCCATGTGCCCAAGAGCTACCGGAGAAGGAGACGCCACAAGAG GAAGGCTGGGCACAAGGAAAAGAAGGAAAAGGAGAGAATCTCCGAGAACTACTCCGACAAATCTGATGTG GAGAATGCGGATGAGTCCAGCAGCAGTATCCTCAAACCCCTCATCTCCCCGGCCGCAGAACGCATCCGAT TCATCTTGGGAGAGGAAGATGACAGCCCGGCACCTCCTCAGCTCTTCACGGAACTCGATGAGCTTCTGGC TGTGGATGGACAGGAGATGGAATGGAAGGAGACAGCGAGGTGGATTAAGTTTGAAGAGAAAGTGGAGCAG GGTGGGGAGCGATGGAGCAAACCCCATGTGGCCACCTTGTCCCTGCACAGCCTGTTTGAGCTGAGGACAT GTATGGAGAAAGGATCCATCATGCTTGACCGGGAGGCATCTTCTCTCCCACAGCTGGTGGAGATGATTGC AGACCACCAGATCGAGACAGGCCTACTGAAGCCTGACCTGAAGGATAAGGTCACCTATACTCTGCTCCGG AAACATCGACATCAAACCAAGAAATCCAACCTTCGGTCCCTGGCTGACATTGGGAAGACTGTCTCCAGTG CAAGCAGCCCAGCCATGACCCACAGGAATCTGACATCCTCCAGTCTCAATGACATTTCTGATAAACCAGA GAAGGATCAGCTGAAGAATAAATTCATGAAAAAACTGCCCCGAGATGCGGAAGCTTCCAATGTGCTTGTT GGGGAGGTTGACTTCTTGGACACTCCCTTCATTGCCTTTGTTCGCCTACAGCAGGCTGTCATGCTGGGTG CCCTGACTGAGGTCCCTGTGCCCACAAGGTTCTTGTTCATTCTCTTAGGTCCAAAGGGGAAAGCCAAGTC CTACCATGAGATTGGAAGAGCTATCGCCACCTTGATGTCTGACGAGGTGTTCCACGACATCGCTTACAAA GCGAAAGACAGACACGACCTGATTGCTGGCATTGATGAGTTCTTAGATGAAGTCATTGTCCTTCCACCTG GGGAATGGGACCCCACAATCCGAATAGAGCCTCCAAAGAGTCTCCCATCATCTGACAAAAGAAAGAATAT GTACTCAGGTGGAGAGAATGTTCAGATGAATGGGGACACACCTCATGATGGAGGCCACGGAGGAGGAGGA CATGGTGACTGTGAAGAACTACAGAGAACTGGCCGGTTCTGCGGTGGATTAATTAAGGACATAAAGAGGA AAGCACCATTTTTTGCCAGTGACTTTTATGATGCTTTAAACATTCAGGCTCTCTCTGCGATTCTCTTCAT TTATCTGGCAACCGTAACCAACGCCATCACTTTTGGAGGCCTGCTCGGGGATGCCACCGACAACATGCAG GGTGTGTTGGAGAGTTTCCTGGGCACTGCTGTCTCCGGAGCCATCTTCTGCCTTTTTGCGGGTCAACCGC TTACCATCTTGAGCAGCACGGGACCAGTGTTGGTGTTTGAGAGGCTTCTTTTTAACTTCAGCAAGGACCA TAATTTTGACTACTTGGAGTTTCGTCTTTGGATTGGCCTGTGGTCAGCCTTCATGTGTCTTGTCCTGGTG GCCACTGATGCGAGCTTCCTGGTTCAGTACTTCACCCGTTTCACAGAAGAAGGTTTCTCCTCTCTCATCA GCTTCATCTTCATCTATGATGCTTTCAAGAAGATGATCAAGCTAGCAGATTACTATCCCATCAACTCTGA CTTCAAAGTGGGTTACAATACTCACTTCTCTTGTGCTTGCCTGCCACCCGACCCAGTTAATCTCTCAGTA TCTAATGATACCACACTAGCCCCAGAGGACCTGCCGACCATTTCTTCTACTGACATGTACCATAATGTCA CCTTTGACTGGGCCTATTTGTCAAAGAAGGAGTGTGTGAAGTATGGAGGGAAGCTCGTGGGAAACAACTG TGACTTCGTGCCTGATATCACACTCATGTCCTTCATTCTCTTCCTGGGCACTTACACCTCGTCTATGGCT ATGAAGAAATTCAAAACCAGTCGCTATTTTCCAACCACAGCAAGAAAACTGATCAGTGATTTTGCCATTA TCCTGTCCATTCTCATATTCTGTGTAATAGATGCCCTAGTCGGCGTGGACACTCCGAAGCTCATTGTACC AAGTGAGTTCAAGCCAACAAGTCCTAACAGGGGTTGGTTTGTCCCGCCATTTGGAGGAAACCCTTGGTGG GTGTGCCTTGCTGCTGCCATCCCGGCTTTGCTAGTCACCATCCTGATTTTCATGGACCAGCAGATCACCG CTGTGATTGTGAACAGGAAAGAGCATAAACTCAAGAAAGGAGCTGGGTATCACCTGGATCTGTTTTGGGT CGCCATCCTCATGGTGGTATGCTCCTTCATGGCTCTTCCCTGGTATGTGGCTGCTACTGTTATCTCCATT GCCCACATTGACAGTCTGAAGATGGAGACGGAGACATCTGCGCCTGGAGAACAACCAAAATTTCTGGGAG TAAGGGAACAACGAGTCACTGGAACCCTTGTGTTTATTCTGACTGGCCTGTCAGTCTTCATGGCTCCCAT CCTGAAGTTTATCCCGATGCCTGTGCTGTATGGTGTGTTCCTGTATATGGGGGTGGCCTCACTTAACGGT GTGCAGTTCATGGACCGTCTCAAGCTGCTGCTGATGCCCTTGAAGCATCAGCCCGACTTCATCTACCTGC GCCACGTCCCCCTCCGCCGAGTCCACCTGTTCACCTTCCTGCAGGTGTTGTGCCTGGCTCTGCTCTGGAT CCTCAAGTCAACAGTGGCTGCAATTATTTTTCCTGTTATGATCCTGGCCCTGGTAGCAGTCAGAAAAGGT ATGGATTACCTCTTCTCCCAGCACGACCTCAGCTTCCTTGATGATGTCATTCCAGAAAAGGACAAGAAAA AGAAGGAGGACGAGAAGAAAAAGAAAAAGAAGAAAGGAAGTTTGGATAGCGACAATGACGATTCTGACTG CCCATACTCAGAAAAGGTCCCCAGTATTAAAATTCCAATGGACATCATGGAACAGCAACCTTTCCTAAGT GATAACAAACCCTTGGACAGAGAAAGATCCTCAACATTCCTCGAACGCCACACATCATGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001197147
Insert Size	3213 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001197147.1, NP_001184076.1
RefSeq Size	7465 bp
RefSeq ORF	3213 bp
Locus ID	54403
Gene Summary	Electrogenic sodium/bicarbonate cotransporter with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks an alternate in-frame exon and uses an alternate in-frame splice junction compared to variant 4, that causes a frameshift. The resulting isoform (c) has the same N- and C-termini but is shorter compared to isoform d. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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