Slc4a4 (NM_001197147) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI835705; NBC; NBC1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231545 representing NM_001197147 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGATGAAGCTGTCCTGGACAGAGGGGCTTCCTTCCTTAAACATGTGTGTGATGAAGAAGAAGTAG AAGGTCACCACACGATCTACATTGGGGTCCATGTGCCCAAGAGCTACCGGAGAAGGAGACGCCACAAGAG GAAGGCTGGGCACAAGGAAAAGAAGGAAAAGGAGAGAATCTCCGAGAACTACTCCGACAAATCTGATGTG GAGAATGCGGATGAGTCCAGCAGCAGTATCCTCAAACCCCTCATCTCCCCGGCCGCAGAACGCATCCGAT TCATCTTGGGAGAGGAAGATGACAGCCCGGCACCTCCTCAGCTCTTCACGGAACTCGATGAGCTTCTGGC TGTGGATGGACAGGAGATGGAATGGAAGGAGACAGCGAGGTGGATTAAGTTTGAAGAGAAAGTGGAGCAG GGTGGGGAGCGATGGAGCAAACCCCATGTGGCCACCTTGTCCCTGCACAGCCTGTTTGAGCTGAGGACAT GTATGGAGAAAGGATCCATCATGCTTGACCGGGAGGCATCTTCTCTCCCACAGCTGGTGGAGATGATTGC AGACCACCAGATCGAGACAGGCCTACTGAAGCCTGACCTGAAGGATAAGGTCACCTATACTCTGCTCCGG AAACATCGACATCAAACCAAGAAATCCAACCTTCGGTCCCTGGCTGACATTGGGAAGACTGTCTCCAGTG CAAGCAGCCCAGCCATGACCCACAGGAATCTGACATCCTCCAGTCTCAATGACATTTCTGATAAACCAGA GAAGGATCAGCTGAAGAATAAATTCATGAAAAAACTGCCCCGAGATGCGGAAGCTTCCAATGTGCTTGTT GGGGAGGTTGACTTCTTGGACACTCCCTTCATTGCCTTTGTTCGCCTACAGCAGGCTGTCATGCTGGGTG CCCTGACTGAGGTCCCTGTGCCCACAAGGTTCTTGTTCATTCTCTTAGGTCCAAAGGGGAAAGCCAAGTC CTACCATGAGATTGGAAGAGCTATCGCCACCTTGATGTCTGACGAGGTGTTCCACGACATCGCTTACAAA GCGAAAGACAGACACGACCTGATTGCTGGCATTGATGAGTTCTTAGATGAAGTCATTGTCCTTCCACCTG GGGAATGGGACCCCACAATCCGAATAGAGCCTCCAAAGAGTCTCCCATCATCTGACAAAAGAAAGAATAT GTACTCAGGTGGAGAGAATGTTCAGATGAATGGGGACACACCTCATGATGGAGGCCACGGAGGAGGAGGA CATGGTGACTGTGAAGAACTACAGAGAACTGGCCGGTTCTGCGGTGGATTAATTAAGGACATAAAGAGGA AAGCACCATTTTTTGCCAGTGACTTTTATGATGCTTTAAACATTCAGGCTCTCTCTGCGATTCTCTTCAT TTATCTGGCAACCGTAACCAACGCCATCACTTTTGGAGGCCTGCTCGGGGATGCCACCGACAACATGCAG GGTGTGTTGGAGAGTTTCCTGGGCACTGCTGTCTCCGGAGCCATCTTCTGCCTTTTTGCGGGTCAACCGC TTACCATCTTGAGCAGCACGGGACCAGTGTTGGTGTTTGAGAGGCTTCTTTTTAACTTCAGCAAGGACCA TAATTTTGACTACTTGGAGTTTCGTCTTTGGATTGGCCTGTGGTCAGCCTTCATGTGTCTTGTCCTGGTG GCCACTGATGCGAGCTTCCTGGTTCAGTACTTCACCCGTTTCACAGAAGAAGGTTTCTCCTCTCTCATCA GCTTCATCTTCATCTATGATGCTTTCAAGAAGATGATCAAGCTAGCAGATTACTATCCCATCAACTCTGA CTTCAAAGTGGGTTACAATACTCACTTCTCTTGTGCTTGCCTGCCACCCGACCCAGTTAATCTCTCAGTA TCTAATGATACCACACTAGCCCCAGAGGACCTGCCGACCATTTCTTCTACTGACATGTACCATAATGTCA CCTTTGACTGGGCCTATTTGTCAAAGAAGGAGTGTGTGAAGTATGGAGGGAAGCTCGTGGGAAACAACTG TGACTTCGTGCCTGATATCACACTCATGTCCTTCATTCTCTTCCTGGGCACTTACACCTCGTCTATGGCT ATGAAGAAATTCAAAACCAGTCGCTATTTTCCAACCACAGCAAGAAAACTGATCAGTGATTTTGCCATTA TCCTGTCCATTCTCATATTCTGTGTAATAGATGCCCTAGTCGGCGTGGACACTCCGAAGCTCATTGTACC AAGTGAGTTCAAGCCAACAAGTCCTAACAGGGGTTGGTTTGTCCCGCCATTTGGAGGAAACCCTTGGTGG GTGTGCCTTGCTGCTGCCATCCCGGCTTTGCTAGTCACCATCCTGATTTTCATGGACCAGCAGATCACCG CTGTGATTGTGAACAGGAAAGAGCATAAACTCAAGAAAGGAGCTGGGTATCACCTGGATCTGTTTTGGGT CGCCATCCTCATGGTGGTATGCTCCTTCATGGCTCTTCCCTGGTATGTGGCTGCTACTGTTATCTCCATT GCCCACATTGACAGTCTGAAGATGGAGACGGAGACATCTGCGCCTGGAGAACAACCAAAATTTCTGGGAG TAAGGGAACAACGAGTCACTGGAACCCTTGTGTTTATTCTGACTGGCCTGTCAGTCTTCATGGCTCCCAT CCTGAAGTTTATCCCGATGCCTGTGCTGTATGGTGTGTTCCTGTATATGGGGGTGGCCTCACTTAACGGT GTGCAGTTCATGGACCGTCTCAAGCTGCTGCTGATGCCCTTGAAGCATCAGCCCGACTTCATCTACCTGC GCCACGTCCCCCTCCGCCGAGTCCACCTGTTCACCTTCCTGCAGGTGTTGTGCCTGGCTCTGCTCTGGAT CCTCAAGTCAACAGTGGCTGCAATTATTTTTCCTGTTATGATCCTGGCCCTGGTAGCAGTCAGAAAAGGT ATGGATTACCTCTTCTCCCAGCACGACCTCAGCTTCCTTGATGATGTCATTCCAGAAAAGGACAAGAAAA AGAAGGAGGACGAGAAGAAAAAGAAAAAGAAGAAAGGAAGTTTGGATAGCGACAATGACGATTCTGACTG CCCATACTCAGAAAAGGTCCCCAGTATTAAAATTCCAATGGACATCATGGAACAGCAACCTTTCCTAAGT GATAACAAACCCTTGGACAGAGAAAGATCCTCAACATTCCTCGAACGCCACACATCATGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231545 representing NM_001197147 Red=Cloning site Green=Tags(s) MEDEAVLDRGASFLKHVCDEEEVEGHHTIYIGVHVPKSYRRRRRHKRKAGHKEKKEKERISENYSDKSDV ENADESSSSILKPLISPAAERIRFILGEEDDSPAPPQLFTELDELLAVDGQEMEWKETARWIKFEEKVEQ GGERWSKPHVATLSLHSLFELRTCMEKGSIMLDREASSLPQLVEMIADHQIETGLLKPDLKDKVTYTLLR KHRHQTKKSNLRSLADIGKTVSSASSPAMTHRNLTSSSLNDISDKPEKDQLKNKFMKKLPRDAEASNVLV GEVDFLDTPFIAFVRLQQAVMLGALTEVPVPTRFLFILLGPKGKAKSYHEIGRAIATLMSDEVFHDIAYK AKDRHDLIAGIDEFLDEVIVLPPGEWDPTIRIEPPKSLPSSDKRKNMYSGGENVQMNGDTPHDGGHGGGG HGDCEELQRTGRFCGGLIKDIKRKAPFFASDFYDALNIQALSAILFIYLATVTNAITFGGLLGDATDNMQ GVLESFLGTAVSGAIFCLFAGQPLTILSSTGPVLVFERLLFNFSKDHNFDYLEFRLWIGLWSAFMCLVLV ATDASFLVQYFTRFTEEGFSSLISFIFIYDAFKKMIKLADYYPINSDFKVGYNTHFSCACLPPDPVNLSV SNDTTLAPEDLPTISSTDMYHNVTFDWAYLSKKECVKYGGKLVGNNCDFVPDITLMSFILFLGTYTSSMA MKKFKTSRYFPTTARKLISDFAIILSILIFCVIDALVGVDTPKLIVPSEFKPTSPNRGWFVPPFGGNPWW VCLAAAIPALLVTILIFMDQQITAVIVNRKEHKLKKGAGYHLDLFWVAILMVVCSFMALPWYVAATVISI AHIDSLKMETETSAPGEQPKFLGVREQRVTGTLVFILTGLSVFMAPILKFIPMPVLYGVFLYMGVASLNG VQFMDRLKLLLMPLKHQPDFIYLRHVPLRRVHLFTFLQVLCLALLWILKSTVAAIIFPVMILALVAVRKG MDYLFSQHDLSFLDDVIPEKDKKKKEDEKKKKKKKGSLDSDNDDSDCPYSEKVPSIKIPMDIMEQQPFLS DNKPLDRERSSTFLERHTSC TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001197147
ORF Size	3210 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001197147.1, NP_001184076.1
RefSeq Size	7465 bp
RefSeq ORF	3213 bp
Locus ID	54403
MW	120.9 kDa
Gene Summary	Electrogenic sodium/bicarbonate cotransporter with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH.[UniProtKB/Swiss-Prot Function]

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