Srgap1 (NM_001242411) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4930572H05Rik; Arhgap13
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229328 representing NM_001242411 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCACCCCGAGCAGATTCAAGAAGGACAAAGAGATCATAGCCGAGTATGAAAGTCAAGTCAAAGAAA TCCGAGCCCAGCTGGTAGAACAACAAAAATGCCTGGAGCAGCAGACAGAGATGCGAGTTCAGCTGCTCCA GGACCTACAAGATTTCTTCCGGAAAAAAGCCGAAATTGAGACGGAATATTCTCGGAACCTGGAGAAGCTG GCAGAAAGGTTCATGGCCAAAACAAGAAGCACTAAGGACCATCAACAGTTCAAGAAAGACCAAAACCTGT TGTCTCCAGTGAACTGCTGGTATTTGCTCCTGAACCAAGTGAGAAGAGAGAGCAAAGACCACGCCACTTT GAGTGACATCTATCTGAACAATGTCATCATGCGGTTCATGCAGATAAGCGAGGACTCCACCCGGATGTTT AAAAAGAGCAAAGAGATTGCGTTCCAGCTTCACGAGGACCTCATGAAGGTTCTGAATGAGCTCTACACGG TGATGAAAACCTACCACATGTACCACTCAGAGAGCATCAGTGCAGAGAGTAAGCTGAAAGAAGCCGAGAA ACAAGAGGAGAAGCAGATTGGGAGGTCCGGCGACCCAGTCTTCCACATTAGGCTGGAAGAGAGGCATCAG AGGCGGAGCTCTGTGAAGAAAATTGAAAAAATGAAGGAAAAGAGACAAGCAAAATATTCAGAAAATAAGC TGAAATCGATCAAAGCCCGCAACGAGTATCTCCTAACACTTGAAGCAACCAACGCCTCCGTGTTCAAGTA TTACATCCATGATCTTTCAGACTTAATAGACTGCTGTGATCTCGGCTACCATGCAAGTCTGAACAGAGCC CTCAGAACGTACCTCTCGGCGGAGTACAACCTTGAAACCTCCAGACACGAAGGCTTAGACATCATTGAAA ACGCTGTGGACAATTTAGAGCCCAGGAGTGACAAGCAGAGGTTCATGGAGATGTACCCTGCGGCATTCTG CCCGCCAATGAAATTCGAGTTTCAGTCTCACATGGGTGATGAGGTGTGTCAGGTCAGCGCCCAGCAGCCC GTCCAGGCGGAGCTCATGCTCAGGAACCAACAGCTGCAGTCCCGACTGGCCACACTCAAGATCGAGAGCG AGGAGGTCAAGAAAACGACAGAAGCCACCCTCCAGACGATCCAAGATATGGTCACCATTGAGGACTATGA TGTGTCTGAGTGTTTCCAGCACAGCCGATCCACAGAGTCTGTGAAGTCCACTGTGTCAGAAACCTACCTG AGTAAACCCAGCATCGCCAAGAGACGAGCCAACCAGCAAGAAACGGAGCAGTTCTACTTCATGAAACTTC GAGAGTTCCTGGAAGGCAGTAACCTCATCACAAAGCTGCAGGCTAAGCATGACTTGCTGCAGAGGACTCT TGGAGAAGGCCATAGAGCTGAATATATGACTACAAGCCGGGGACGAAGGAACTCTCACGCAAGACATCAG GACTCAGGCCAGGTTATCCCTCTCATTGTGGAAAGCTGCATCCGATTCATTAATCTCTATGGCCTTCAGC ACCAGGGGATTTTCAGAGTGTCTGGCTCCCAGGTGGAAGTGAACGATATCAAGAATTCTTTCGAGAGAGG TGAGAACCCTCTGTCCGATGAGCAGAGCAACCACGATATCAACTCGGTGGCCGGCGTGCTGAAGCTGTAC TTCCGAGGGCTGGAAAACCCCCTCTTTCCTAAGGAAAGGTTTACTGACCTGATTTCCTGTATCAGAATAG ATAACCTCTATGAGAGGGCGCTCCACATCCGCAAACTCCTCCTGACCTTGCCCAGGTCGGTCCTTATAGT GATGAGGTACCTGTTTGCCTTCCTCAATCACCTCTCCCAGTACAGTGACGAGAACATGATGGACCCTTAC AATCTGGCCATTTGCTTCGGCCCAACACTGATGCCGGTTCCAGAGATACAGGACCAAGTGTCTTGCCAGG CACACGTGAACGAGATTGTGAAGACCATCATCATCCATCACGAGACTATTTTCCCAGATGCTAAAGAACT GGACGGCCCCGTGTATGAGAAATGCATGGCCGGAGGCGACTACTGCGACAGCCCCTACAGTGAGCATGGT ACATTGGAGGAGGTGGACCAAGACGCCGGCACAGAGCCCCACACCAGTGAAGACGAGTGTGAGCCCATCG AAGCCATAGCCAAGTTTGACTACGTCGGGCGGTCAGCTAGAGAGCTGTCCTTCAAGAAGGGTGCATCCCT GCTGCTGTATCACCGAGCCTCCGAGGATTGGTGGGAAGGCAGACACAACGGGATCGATGGCCTTGTGCCT CATCAGTACATAGTTGTGCAAGATATGGACGATACATTTTCAGACACGCTGAGCCAAAAGGCCGACAGCG AAGCCAGCAGCGGGCCAGTCACTGAAGACAAGTCCTCATCCAAGGACATGAACTCCCCGACTGACCGCCA TTCTGATAGCTATTTAGCCAGACAAAGAAAGAGAGGAGAGCCACCCCCTCCGGGAAGACGTCCCGGCAGG ACCAGCGATGGCCATTGTCCCCTGCATCCCCCGCATGCTCTGTCTAACTCCTCAATTGATCTGGGATCCC CAAACCTAGCCAGTCACCCCAGGGGCCTGTTGCAGAACCGTGGCCTCAATAATGACAGTCCTGAGAGGCG GCGCCGGCCGGGCCACGGCAGCCTGACCAATATCAGCCGGCATGACTCCCTCAAGAAGATCGACAGCCCT CCTATCCGGAGGTCCACATCATCAGGGCAGTACACAGGCTTCAATGACCACAAGCCACTGGACCCCGAGA CGATTGCTCAGGATATTGAGGAGACCATGAACACTGCTTTGAACGAACTCCGTGAACTCGAGAGGCAGAG CACAGTGAAGCACGCCCCCGATGTCGTGCTAGACACTTTGGAGCAAGTGAAGAACTCGCCCACCCCTGCG ACTTCCACAGAATCACTCAGCCCACTGCACAATGTCGCCCTCAGGGGCTCAGAGCCACAGATTCGCCGGA GCACCAGCTCCTCCAGCGAGACAATGAGTACTTTCAAGCCTATGGTGGCGCCCCGGATGGGCGTCCAGCT GAAGCCCCCAGCCCTTAGGCCAAAGCCTGCTGTTCTTCCAAAAACAAACCCTACGATGGGACCTGCCGCA CCTTCCCAGGGTCCCACAGACAAGTCTTGCACGATGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001242411
Insert Size	3189 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001242411.1, NP_001229340.1
RefSeq Size	7718 bp
RefSeq ORF	3189 bp
Locus ID	117600
UniProt ID	Q91Z69
Gene Summary	GTPase-activating protein for RhoA and Cdc42 small GTPases. Together with CDC42 seems to be involved in the pathway mediating the repulsive signaling of Robo and Slit proteins in neuronal migration. SLIT2, probably through interaction with ROBO1, increases the interaction of SRGAP1 with ROBO1 and inactivates CDC42 (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate coding exon, that causes a frameshift; however, it contains an alternate downstream exon that restores the reading frame. The resulting isoform (2) has a different internal segment compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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