Srgap1 (NM_001242411) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	4930572H05Rik; Arhgap13
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231539 representing NM_001242411 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCACCCCGAGCAGATTCAAGAAGGACAAAGAGATCATAGCCGAGTATGAAAGTCAAGTCAAAGAAA TCCGAGCCCAGCTGGTAGAACAACAAAAATGCCTGGAGCAGCAGACAGAGATGCGAGTTCAGCTGCTCCA GGACCTACAAGATTTCTTCCGGAAAAAAGCCGAAATTGAGACGGAATATTCTCGGAACCTGGAGAAGCTG GCAGAAAGGTTCATGGCCAAAACAAGAAGCACTAAGGACCATCAACAGTTCAAGAAAGACCAAAACCTGT TGTCTCCAGTGAACTGCTGGTATTTGCTCCTGAACCAAGTGAGAAGAGAGAGCAAAGACCACGCCACTTT GAGTGACATCTATCTGAACAATGTCATCATGCGGTTCATGCAGATAAGCGAGGACTCCACCCGGATGTTT AAAAAGAGCAAAGAGATTGCGTTCCAGCTTCACGAGGACCTCATGAAGGTTCTGAATGAGCTCTACACGG TGATGAAAACCTACCACATGTACCACTCAGAGAGCATCAGTGCAGAGAGTAAGCTGAAAGAAGCCGAGAA ACAAGAGGAGAAGCAGATTGGGAGGTCCGGCGACCCAGTCTTCCACATTAGGCTGGAAGAGAGGCATCAG AGGCGGAGCTCTGTGAAGAAAATTGAAAAAATGAAGGAAAAGAGACAAGCAAAATATTCAGAAAATAAGC TGAAATCGATCAAAGCCCGCAACGAGTATCTCCTAACACTTGAAGCAACCAACGCCTCCGTGTTCAAGTA TTACATCCATGATCTTTCAGACTTAATAGACTGCTGTGATCTCGGCTACCATGCAAGTCTGAACAGAGCC CTCAGAACGTACCTCTCGGCGGAGTACAACCTTGAAACCTCCAGACACGAAGGCTTAGACATCATTGAAA ACGCTGTGGACAATTTAGAGCCCAGGAGTGACAAGCAGAGGTTCATGGAGATGTACCCTGCGGCATTCTG CCCGCCAATGAAATTCGAGTTTCAGTCTCACATGGGTGATGAGGTGTGTCAGGTCAGCGCCCAGCAGCCC GTCCAGGCGGAGCTCATGCTCAGGAACCAACAGCTGCAGTCCCGACTGGCCACACTCAAGATCGAGAGCG AGGAGGTCAAGAAAACGACAGAAGCCACCCTCCAGACGATCCAAGATATGGTCACCATTGAGGACTATGA TGTGTCTGAGTGTTTCCAGCACAGCCGATCCACAGAGTCTGTGAAGTCCACTGTGTCAGAAACCTACCTG AGTAAACCCAGCATCGCCAAGAGACGAGCCAACCAGCAAGAAACGGAGCAGTTCTACTTCATGAAACTTC GAGAGTTCCTGGAAGGCAGTAACCTCATCACAAAGCTGCAGGCTAAGCATGACTTGCTGCAGAGGACTCT TGGAGAAGGCCATAGAGCTGAATATATGACTACAAGCCGGGGACGAAGGAACTCTCACGCAAGACATCAG GACTCAGGCCAGGTTATCCCTCTCATTGTGGAAAGCTGCATCCGATTCATTAATCTCTATGGCCTTCAGC ACCAGGGGATTTTCAGAGTGTCTGGCTCCCAGGTGGAAGTGAACGATATCAAGAATTCTTTCGAGAGAGG TGAGAACCCTCTGTCCGATGAGCAGAGCAACCACGATATCAACTCGGTGGCCGGCGTGCTGAAGCTGTAC TTCCGAGGGCTGGAAAACCCCCTCTTTCCTAAGGAAAGGTTTACTGACCTGATTTCCTGTATCAGAATAG ATAACCTCTATGAGAGGGCGCTCCACATCCGCAAACTCCTCCTGACCTTGCCCAGGTCGGTCCTTATAGT GATGAGGTACCTGTTTGCCTTCCTCAATCACCTCTCCCAGTACAGTGACGAGAACATGATGGACCCTTAC AATCTGGCCATTTGCTTCGGCCCAACACTGATGCCGGTTCCAGAGATACAGGACCAAGTGTCTTGCCAGG CACACGTGAACGAGATTGTGAAGACCATCATCATCCATCACGAGACTATTTTCCCAGATGCTAAAGAACT GGACGGCCCCGTGTATGAGAAATGCATGGCCGGAGGCGACTACTGCGACAGCCCCTACAGTGAGCATGGT ACATTGGAGGAGGTGGACCAAGACGCCGGCACAGAGCCCCACACCAGTGAAGACGAGTGTGAGCCCATCG AAGCCATAGCCAAGTTTGACTACGTCGGGCGGTCAGCTAGAGAGCTGTCCTTCAAGAAGGGTGCATCCCT GCTGCTGTATCACCGAGCCTCCGAGGATTGGTGGGAAGGCAGACACAACGGGATCGATGGCCTTGTGCCT CATCAGTACATAGTTGTGCAAGATATGGACGATACATTTTCAGACACGCTGAGCCAAAAGGCCGACAGCG AAGCCAGCAGCGGGCCAGTCACTGAAGACAAGTCCTCATCCAAGGACATGAACTCCCCGACTGACCGCCA TTCTGATAGCTATTTAGCCAGACAAAGAAAGAGAGGAGAGCCACCCCCTCCGGGAAGACGTCCCGGCAGG ACCAGCGATGGCCATTGTCCCCTGCATCCCCCGCATGCTCTGTCTAACTCCTCAATTGATCTGGGATCCC CAAACCTAGCCAGTCACCCCAGGGGCCTGTTGCAGAACCGTGGCCTCAATAATGACAGTCCTGAGAGGCG GCGCCGGCCGGGCCACGGCAGCCTGACCAATATCAGCCGGCATGACTCCCTCAAGAAGATCGACAGCCCT CCTATCCGGAGGTCCACATCATCAGGGCAGTACACAGGCTTCAATGACCACAAGCCACTGGACCCCGAGA CGATTGCTCAGGATATTGAGGAGACCATGAACACTGCTTTGAACGAACTCCGTGAACTCGAGAGGCAGAG CACAGTGAAGCACGCCCCCGATGTCGTGCTAGACACTTTGGAGCAAGTGAAGAACTCGCCCACCCCTGCG ACTTCCACAGAATCACTCAGCCCACTGCACAATGTCGCCCTCAGGGGCTCAGAGCCACAGATTCGCCGGA GCACCAGCTCCTCCAGCGAGACAATGAGTACTTTCAAGCCTATGGTGGCGCCCCGGATGGGCGTCCAGCT GAAGCCCCCAGCCCTTAGGCCAAAGCCTGCTGTTCTTCCAAAAACAAACCCTACGATGGGACCTGCCGCA CCTTCCCAGGGTCCCACAGACAAGTCTTGCACGATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231539 representing NM_001242411 Red=Cloning site Green=Tags(s) MSTPSRFKKDKEIIAEYESQVKEIRAQLVEQQKCLEQQTEMRVQLLQDLQDFFRKKAEIETEYSRNLEKL AERFMAKTRSTKDHQQFKKDQNLLSPVNCWYLLLNQVRRESKDHATLSDIYLNNVIMRFMQISEDSTRMF KKSKEIAFQLHEDLMKVLNELYTVMKTYHMYHSESISAESKLKEAEKQEEKQIGRSGDPVFHIRLEERHQ RRSSVKKIEKMKEKRQAKYSENKLKSIKARNEYLLTLEATNASVFKYYIHDLSDLIDCCDLGYHASLNRA LRTYLSAEYNLETSRHEGLDIIENAVDNLEPRSDKQRFMEMYPAAFCPPMKFEFQSHMGDEVCQVSAQQP VQAELMLRNQQLQSRLATLKIESEEVKKTTEATLQTIQDMVTIEDYDVSECFQHSRSTESVKSTVSETYL SKPSIAKRRANQQETEQFYFMKLREFLEGSNLITKLQAKHDLLQRTLGEGHRAEYMTTSRGRRNSHARHQ DSGQVIPLIVESCIRFINLYGLQHQGIFRVSGSQVEVNDIKNSFERGENPLSDEQSNHDINSVAGVLKLY FRGLENPLFPKERFTDLISCIRIDNLYERALHIRKLLLTLPRSVLIVMRYLFAFLNHLSQYSDENMMDPY NLAICFGPTLMPVPEIQDQVSCQAHVNEIVKTIIIHHETIFPDAKELDGPVYEKCMAGGDYCDSPYSEHG TLEEVDQDAGTEPHTSEDECEPIEAIAKFDYVGRSARELSFKKGASLLLYHRASEDWWEGRHNGIDGLVP HQYIVVQDMDDTFSDTLSQKADSEASSGPVTEDKSSSKDMNSPTDRHSDSYLARQRKRGEPPPPGRRPGR TSDGHCPLHPPHALSNSSIDLGSPNLASHPRGLLQNRGLNNDSPERRRRPGHGSLTNISRHDSLKKIDSP PIRRSTSSGQYTGFNDHKPLDPETIAQDIEETMNTALNELRELERQSTVKHAPDVVLDTLEQVKNSPTPA TSTESLSPLHNVALRGSEPQIRRSTSSSSETMSTFKPMVAPRMGVQLKPPALRPKPAVLPKTNPTMGPAA PSQGPTDKSCTM TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001242411
ORF Size	3186 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001242411.1, NP_001229340.1
RefSeq Size	7718 bp
RefSeq ORF	3189 bp
Locus ID	117600
UniProt ID	Q91Z69
MW	121.9 kDa
Gene Summary	GTPase-activating protein for RhoA and Cdc42 small GTPases. Together with CDC42 seems to be involved in the pathway mediating the repulsive signaling of Robo and Slit proteins in neuronal migration. SLIT2, probably through interaction with ROBO1, increases the interaction of SRGAP1 with ROBO1 and inactivates CDC42 (By similarity).[UniProtKB/Swiss-Prot Function]

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