Nox4 (NM_001285833) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI648021
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228242 representing NM_001285833 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGCCGGACAGTCCTGGCTTATCTTCGAGGATCACAGAAGGTCCCTAGCAGGAGAACAAGAAGATTGT TGGATAAAAGCAAGACTCTACACATCACATGTGGTGTAACTATCTGTATTTTCTCAGGTGTGCATGTAGC CGCCCACTTGGTGAATGCCCTCAACTTTTCAGTGAACTACAGTGAAGATTTCCTTGAACTGAATGCAGCA AGATACCAGAATGAGGATCCCAGAAAGCTTCTCTTCACAACCATTCCTGGTCTGACGGGTGTCTGCATGG TGGTGGTATTGTTCCTCATGGTTACAGCTTCTACCTACGCAATAAGAGTTTCTAATTATGATATCTTCTG GTATACTCACAACCTCTTCTTTGTCTTCTACATGCTGCTGCTGTTGCATGTTTCAGGTGGTTTGTTGAAG TATCAGACAAATGTAGACACTCACCCTCCTGGCTGCATTAGTCTTAACCAGACATCATCCCAGAATATGT CCATACCAGACTACGTCTCAGAACATTTTCATGGATCTTTGCCTCGAGGGTTTTCAAAATTAGAAGATCG TTACCAGAAAACACTTGTGAAGATTTGCCTGGAAGAACCCAAGTTCCAAGCTCATTTCCCACAGACCTGG ATTTGGATTTCTGGACCTTTGTGCCTTTATTGTGCGGAGAGACTTTACCGATGCATCAGGAGCAACAAAC CTGTCACCATCATCTCAGTCATCAATCATCCCTCTGATGTAATGGAACTCCGTATGATCAAAGAAAACTT TAAAGCAAGACCTGGCCAGTATATTATTCTCCATTGCCCCAGTGTATCAGCATTAGAAAACCACCCATTT ACTCTCACAATGCTCTGTCAGAAACGATGGCAGCCCTGGCACAGCAGAGTATGGATATACAGTTTCTTTG TCTGTAGTGATGCTGCATGCCATGAAGATACTTCCAAGATGAACCATGCCTTCTTACTGTGTCCTACTGA AACCAAAGCAACATTTGGTGTCCACTTTAAAGTAGTAGGAGACTGGACAGAACGATTCCGGGATTTGCTA CTGCCTCCATCAAGTCAAGACTCTGAGATTCTGCCCTTCATTCACTCTAGAAATTACCCTAAGTTATACA TTGATGGTCCATTTGGAAGCCCATTTGAGGAGTCACTGAACTATGAAGTTAGTCTCTGTGTGGCTGGAGG CATTGGAGTCACTCCATTTGCATCGATACTAAACACTCTACTGGATGACTGGAAACCATACAAGTTAAGA AGACTGTACTTTATCTGGGTGTGCAGAGACATCCAATCATTCCAGTGGTTTGCAGATTTACTCTGTGTGT TGCATAACAAGTTTTGGCAAGAAAACAGACCTGACTTTGTGAACATCCAGCTGTACCTCAGTCAAACAGA TGGGATTCAGAAGATAATTGGAGAAAAATATCACACACTGAATTCGAGACTTTTCATTGGGCGTCCTCGG TGGAAACTTTTATTTGATGAAATAGCAAAATGTAACAGAGGGAAAACAGTTGGAGTTTTCTGCTGTGGAC CCAGTTCTATTTCCAAGACTCTTCATAGTTTGAGTAACCGGAACAACTCATATGGGACAAAATTTGAATA CAATAAAGAATCCTTCAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001285833
Insert Size	1632 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001285833.1, NP_001272762.1
RefSeq Size	3873 bp
RefSeq ORF	1632 bp
Locus ID	50490
Gene Summary	Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate splice junction at the 5' end of an exon and contains two additional alternate coding exons compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus and an alternate internal segment compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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