Nox4 (NM_001285833) Mouse Tagged ORF Clone
CAT#: MR230453
- TrueORF®
Nox4 (myc-DDK-tagged) - Mouse NADPH oxidase 4 (Nox4), transcript variant 2
"NM_001285833" in other vectors (1)
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CNY 5,420.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AI648021 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR230453 representing NM_001285833
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGCCGGACAGTCCTGGCTTATCTTCGAGGATCACAGAAGGTCCCTAGCAGGAGAACAAGAAGATTGT TGGATAAAAGCAAGACTCTACACATCACATGTGGTGTAACTATCTGTATTTTCTCAGGTGTGCATGTAGC CGCCCACTTGGTGAATGCCCTCAACTTTTCAGTGAACTACAGTGAAGATTTCCTTGAACTGAATGCAGCA AGATACCAGAATGAGGATCCCAGAAAGCTTCTCTTCACAACCATTCCTGGTCTGACGGGTGTCTGCATGG TGGTGGTATTGTTCCTCATGGTTACAGCTTCTACCTACGCAATAAGAGTTTCTAATTATGATATCTTCTG GTATACTCACAACCTCTTCTTTGTCTTCTACATGCTGCTGCTGTTGCATGTTTCAGGTGGTTTGTTGAAG TATCAGACAAATGTAGACACTCACCCTCCTGGCTGCATTAGTCTTAACCAGACATCATCCCAGAATATGT CCATACCAGACTACGTCTCAGAACATTTTCATGGATCTTTGCCTCGAGGGTTTTCAAAATTAGAAGATCG TTACCAGAAAACACTTGTGAAGATTTGCCTGGAAGAACCCAAGTTCCAAGCTCATTTCCCACAGACCTGG ATTTGGATTTCTGGACCTTTGTGCCTTTATTGTGCGGAGAGACTTTACCGATGCATCAGGAGCAACAAAC CTGTCACCATCATCTCAGTCATCAATCATCCCTCTGATGTAATGGAACTCCGTATGATCAAAGAAAACTT TAAAGCAAGACCTGGCCAGTATATTATTCTCCATTGCCCCAGTGTATCAGCATTAGAAAACCACCCATTT ACTCTCACAATGCTCTGTCAGAAACGATGGCAGCCCTGGCACAGCAGAGTATGGATATACAGTTTCTTTG TCTGTAGTGATGCTGCATGCCATGAAGATACTTCCAAGATGAACCATGCCTTCTTACTGTGTCCTACTGA AACCAAAGCAACATTTGGTGTCCACTTTAAAGTAGTAGGAGACTGGACAGAACGATTCCGGGATTTGCTA CTGCCTCCATCAAGTCAAGACTCTGAGATTCTGCCCTTCATTCACTCTAGAAATTACCCTAAGTTATACA TTGATGGTCCATTTGGAAGCCCATTTGAGGAGTCACTGAACTATGAAGTTAGTCTCTGTGTGGCTGGAGG CATTGGAGTCACTCCATTTGCATCGATACTAAACACTCTACTGGATGACTGGAAACCATACAAGTTAAGA AGACTGTACTTTATCTGGGTGTGCAGAGACATCCAATCATTCCAGTGGTTTGCAGATTTACTCTGTGTGT TGCATAACAAGTTTTGGCAAGAAAACAGACCTGACTTTGTGAACATCCAGCTGTACCTCAGTCAAACAGA TGGGATTCAGAAGATAATTGGAGAAAAATATCACACACTGAATTCGAGACTTTTCATTGGGCGTCCTCGG TGGAAACTTTTATTTGATGAAATAGCAAAATGTAACAGAGGGAAAACAGTTGGAGTTTTCTGCTGTGGAC CCAGTTCTATTTCCAAGACTCTTCATAGTTTGAGTAACCGGAACAACTCATATGGGACAAAATTTGAATA CAATAAAGAATCCTTCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR230453 representing NM_001285833
Red=Cloning site Green=Tags(s) MCRTVLAYLRGSQKVPSRRTRRLLDKSKTLHITCGVTICIFSGVHVAAHLVNALNFSVNYSEDFLELNAA RYQNEDPRKLLFTTIPGLTGVCMVVVLFLMVTASTYAIRVSNYDIFWYTHNLFFVFYMLLLLHVSGGLLK YQTNVDTHPPGCISLNQTSSQNMSIPDYVSEHFHGSLPRGFSKLEDRYQKTLVKICLEEPKFQAHFPQTW IWISGPLCLYCAERLYRCIRSNKPVTIISVINHPSDVMELRMIKENFKARPGQYIILHCPSVSALENHPF TLTMLCQKRWQPWHSRVWIYSFFVCSDAACHEDTSKMNHAFLLCPTETKATFGVHFKVVGDWTERFRDLL LPPSSQDSEILPFIHSRNYPKLYIDGPFGSPFEESLNYEVSLCVAGGIGVTPFASILNTLLDDWKPYKLR RLYFIWVCRDIQSFQWFADLLCVLHNKFWQENRPDFVNIQLYLSQTDGIQKIIGEKYHTLNSRLFIGRPR WKLLFDEIAKCNRGKTVGVFCCGPSSISKTLHSLSNRNNSYGTKFEYNKESFS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001285833 |
ORF Size | 1629 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001285833.1, NP_001272762.1 |
RefSeq Size | 3873 bp |
RefSeq ORF | 1632 bp |
Locus ID | 50490 |
MW | 63.2 kDa |
Gene Summary | Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC228242 | Nox4 (untagged) - Mouse NADPH oxidase 4 (Nox4), transcript variant 2 |
CNY 5,230.00 |