Unkl (NM_001290736) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1300004G08Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228236 representing NM_001290736 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCGAGAAGATTCTTGGTGAGGACCCTCGGTGGCAAGACAGTAACTTCGTATTGGGCAGCTATAAGA CTGAGCAGTGCCCGAAGCCACCACGCCTATGTCGCCAGGGCTACGCCTGTCCACACTACCACAACAGCAG AGACCGCCGTCGGAACCCCAGGAGGTTCCAGTACAGGTCTACGCCCTGTCCTAGTGTGAAGCACGGTGAC GAGTGGGGCGAGCCCTCACGGTGTGACGGTGGGGACAGCTGTCAGTACTGCCATTCCCGCACGGAGCAGC AGTTCCATCCTGAGATCTACAAATCTACAAAATGCAACGATATGCGCCAAACTGGCTACTGCCCTCGGGG CCCTTTCTGTGCCTTTGCACACACTGAGAAGAGCCTGGCTATGGTGAACGAGTGGAGCTGCCGTGATCTC AGTTCCAACAGCACCTCAGCTTATAGCAGCCAGCCTGGAAGTGCCAAAAGGAAAGACTCCCCCTCTGAAG GAAGCCAGAAAGCTACTGAGGACAGCAAGCAGAACCACCTCGCTGTGTTCTCAGTGGCTCACCCACTGGC CCACAGCATAAGCTCCAGTGTGGCATCCAGCCTGGCGTCCAGCACTGGCTCGGGCAGCTCCTCCCCCACC ACACTGCCTACCCTCCCTGCCCGTGCCCTTCCACTGGATCCCGCCGGCAACACTGTGGGGGCAGTCATAG GTTCTGCCTTAGATCTTCGTCTTAGTGACATAAACATCGCGTCCTTAGATAAAGACTTAGAAGAACAGGA CCTTGGATTGACAGGTCCCAGGTCGCTGGCAGGCTCAGCACCCGTCACCATTCCGGGCTCCCTGCCCCGC TCGCCATCCTTGCACTCGTCCTCATCCCTATCCACCTCCCCACTCAGCTCGCTCTCCCAGTCCCTGTCGG GGCCACTCGTCTCCTCGGCCATGACGCCCCCCCAGCAGCCACCACCCCTCCGGTCGGAGCCGGCTACGCT GGGCTCTGCAGCCTCATCTTACAGCTCTTTAGGTCTGAACGGCGTTCCCGGGAGCATCTGGGACTTTGTT TCTGGCAGCTTCTCTCCTAGCCCATCCCCCATTCTGAACTCCGGCCCTTCGGCTTCCTCAAGTGCAAGTC CAAACAGTGCTGAGCTGGCACGGGTCAGGCGGCAGCTAGACGAGGCCAAGAGGAAGATCAGGCAATGGGA AGAATCTTGGCAGCAAGTGAAGCAGGCCTGTGATGCCTGGCAGAGAGAGGCTCAGGAGGCCAAGGAAAGA GCCCGTGTGGCAGACAGTGACCGGCAGCTGGCCCTGCAGAGGAAAGAAGAGGTGGAAGCCAAGGTGAAGC AGCTGCAGGAAGAATTAGAGGGCCTGGGTTTGTCCTCACTGCCAGGGCTTCAGAGCTTGGGTGACATCAG TGACATCCCTCTCCCCAAGCTGCATTCTCTCCAGAGCAAGCTGCGCTTGGACTTGGAGGCTGTGGATGGG GTGATCTTCCAGCTCCGTGCCAAACAGTGTGTGGCCTGCCAGGAGCGGGCCCATGGCACTGTCCTGCGGC CCTGCCAGCACCGTGTCTTATGCGAACCATGTGCGGCCAGTACCCCTGAGTGCCCCTACTGCAAGGGCCA GCCCCTACCATGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290736
Insert Size	1626 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290736.1, NP_001277665.1
RefSeq Size	4787 bp
RefSeq ORF	1626 bp
Locus ID	74154
Gene Summary	May participate in a protein complex showing an E3 ligase activity regulated by Rac1. Ubiquitination is directed towards itself and possibly other substrates, such as Baf60b/Smarcd2. Intrinsic E3 ligase activity has not been proven.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) contains an alternate exon in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon compared to variant 1. The encoded protein (isoform 3) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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