Eef1d (NM_001285430) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	EF-1-delta
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226764 representing NM_001285430 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTACAAACTTTCTAGCGCATGAGAAGATCTGGTTTGACAAGTTTAAATATGATGATGCAGAAAGGA GATTCTATGAGCAGATGAACGGGCCTGTGACCTCCGGCTCCCGCCAGGAGAATGGTGCCAGCGTGATCCT CCGAGACATTGCAAGAGCCAGAGAGAACATCCAGAAATCCTTGGCTGGAAGCTCAGGCCCTGGAGCCTCC AGTGGACCTGGTGGAGACCACAGTGAGCTCATTGTGAGGATTACCAGTCTGGAAGTGGAGAACCAGAACC TTCGAGGCGTGGTGCAAGATTTGCAGCAGGCCATTTCCAAGTTGGAGGCCCGGCTGAGCTCTCTAGAGAA GAGTTCACCTACTCCCCGAGCCACGGCCCCACAGACCCAACATGTCTCTCCTATGCGTCAAGTGGAGCCC CCAACCAAGAAAGGAGCCACACCAGCAGAGGACGATGAGGACAAGGACATTGACCTGTTCGGCAGTGACG AGGAGGAAGAAGATAAGGAGGCTGCCCGACTACGGGAGGAGAGGCTACGCCAGTACGCAGAGAAGAAGGC CAAGAAGCCCACACTGGTGGCCAAATCCTCCATCCTTTTGGATGTTAAACCTTGGGATGATGAGACTGAC ATGGCCCAGCTAGAGACTTGTGTGCGTTCCATCCAATTGGACGGGCTGGTTTGGGGGGCCTCCAAGCTTG TGCCTGTTGGCTATGGCATCCGGAAGCTGCAGATCCAGTGTGTGGTGGAGGATGACAAAGTGGGCACCGA CTTGCTCGAGGAGGAGATCACCAAATTTGAGGAGCATGTGCAGAGTGTCGACATCGCAGCTTTCAACAAG ATCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001285430
Insert Size	846 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001285430.1, NP_001272359.1
RefSeq Size	1707 bp
RefSeq ORF	846 bp
Locus ID	66656
UniProt ID	P57776
Gene Summary	Isoform 1: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (b) has a shorter N-terminus, compared to isoform a. Variants 2, 3, and 4 encode the same isoform.

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