Eef1d (NM_001285430) Mouse Tagged ORF Clone
CAT#: MR228975
- TrueORF®
Eef1d (myc-DDK-tagged) - Mouse eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) (Eef1d), transcript variant 4
"NM_001285430" in other vectors (1)
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CNY 2,850.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | EF-1-delta |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR228975 representing NM_001285430
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTACAAACTTTCTAGCGCATGAGAAGATCTGGTTTGACAAGTTTAAATATGATGATGCAGAAAGGA GATTCTATGAGCAGATGAACGGGCCTGTGACCTCCGGCTCCCGCCAGGAGAATGGTGCCAGCGTGATCCT CCGAGACATTGCAAGAGCCAGAGAGAACATCCAGAAATCCTTGGCTGGAAGCTCAGGCCCTGGAGCCTCC AGTGGACCTGGTGGAGACCACAGTGAGCTCATTGTGAGGATTACCAGTCTGGAAGTGGAGAACCAGAACC TTCGAGGCGTGGTGCAAGATTTGCAGCAGGCCATTTCCAAGTTGGAGGCCCGGCTGAGCTCTCTAGAGAA GAGTTCACCTACTCCCCGAGCCACGGCCCCACAGACCCAACATGTCTCTCCTATGCGTCAAGTGGAGCCC CCAACCAAGAAAGGAGCCACACCAGCAGAGGACGATGAGGACAAGGACATTGACCTGTTCGGCAGTGACG AGGAGGAAGAAGATAAGGAGGCTGCCCGACTACGGGAGGAGAGGCTACGCCAGTACGCAGAGAAGAAGGC CAAGAAGCCCACACTGGTGGCCAAATCCTCCATCCTTTTGGATGTTAAACCTTGGGATGATGAGACTGAC ATGGCCCAGCTAGAGACTTGTGTGCGTTCCATCCAATTGGACGGGCTGGTTTGGGGGGCCTCCAAGCTTG TGCCTGTTGGCTATGGCATCCGGAAGCTGCAGATCCAGTGTGTGGTGGAGGATGACAAAGTGGGCACCGA CTTGCTCGAGGAGGAGATCACCAAATTTGAGGAGCATGTGCAGAGTGTCGACATCGCAGCTTTCAACAAG ATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR228975 representing NM_001285430
Red=Cloning site Green=Tags(s) MATNFLAHEKIWFDKFKYDDAERRFYEQMNGPVTSGSRQENGASVILRDIARARENIQKSLAGSSGPGAS SGPGGDHSELIVRITSLEVENQNLRGVVQDLQQAISKLEARLSSLEKSSPTPRATAPQTQHVSPMRQVEP PTKKGATPAEDDEDKDIDLFGSDEEEEDKEAARLREERLRQYAEKKAKKPTLVAKSSILLDVKPWDDETD MAQLETCVRSIQLDGLVWGASKLVPVGYGIRKLQIQCVVEDDKVGTDLLEEEITKFEEHVQSVDIAAFNK I TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001285430 |
ORF Size | 843 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001285430.1, NP_001272359.1 |
RefSeq Size | 1707 bp |
RefSeq ORF | 846 bp |
Locus ID | 66656 |
UniProt ID | P57776 |
MW | 31.7 kDa |
Gene Summary | Isoform 1: EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC226764 | Eef1d (untagged) - Mouse eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein) (Eef1d), transcript variant 4 |
CNY 2,760.00 |