Txlng (NM_001290777) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4932441K18Rik; Fiat; Lrp
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC226516 representing NM_001290777 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACTCGGCTTGAGGAGGTAACGCGAGGAAGAGGCGGCGGTACTGAGGAGGCTAGTGAGGGCGGAC GGGGCGGACGGCGACGTAGCCCCCCGCAGAAGTTTGAAATTGGCACAATGGAAGAAGCTAGAATTTGTGG GTTAGGAGTAAAAGCAGACATGGAGAAAAGAGAAGAAATTCCTGGACGAGAAGCTCGAACAGGTCCTCCT GATGGCCAGCAAGATTCAGAGTGCAGCAGGAACAAAGAGAAGACCTTAGGAAAAGAAGTTTTATTACTGA TGCAAGCGCTAAACACCCTTTCAACCCCAGAGGAGAAGCTGGCAGCTCTCTGTAAGAAATATGCTGATCT CCTGGAAGAAAGCAGGAATGTTCAGAAACAAATGAAGATTCTGCAGAAGAAGCAAGCCCAGATTGTGAAA GAGAAAGTTCACCTTCAGAGTGAACACAGCAAGGCCATCTTGGCAAGAAGCAAACTGGAATCTCTTTGCA GGGAACTTCAGCGTCATAATAAGACCTTAAAGGAGGAGAATATGCAGCAGGCACGAGAGGAGGAAGAACG ACGTAAAGAAGCAACAGCACATTTCCAGATAACTCTAAATGAAATCCAAGCTCAGTTGGAACAACATGAC ATCCACAATGCCAAACTGCGACAGGAGAACATTGAACTGGGAGAGAAGTTGAAGAAGCTTATTGAGCAGT ATGCACTAAGGGAAGAGGTAATATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290777
Insert Size	726 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290777.1, NP_001277706.1
RefSeq Size	2653 bp
RefSeq ORF	726 bp
Locus ID	353170
UniProt ID	Q8BHN1
Gene Summary	May be involved in intracellular vesicle traffic (By similarity). Inhibits ATF4-mediated transcription, possibly by dimerizing with ATF4 to form inactive dimers that cannot bind DNA. May be involved in regulating bone mass density through an ATF4-dependent pathway. May be involved in cell cycle progression.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) contains multiple differences compared to variant 1, including differing in the 3' coding region and 3' UTR. The encoded isoform (3) has a distinct C-terminus and is shorter than isoform 1.

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