Fn1 (NM_010233) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	E330027I09; Fn; Fn-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225330 representing NM_010233 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCAGGGGTCCGGGACCCGGGCGGCTGCTGCTGCTGGCAGTCCTGTGCCTGGGGACCTCGGTGCGCT GCACCGAAGCCGGGAAGAGCAAGAGGCAGGCTCAGCAAATCGTGCAGCCTCAATCCCCGGTGGCTGTCAG TCAGAGCAAGCCTGGCTGTTTTGACAATGGGAAGCACTATCAGATAAATCAGCAGTGGGAACGGACCTAC CTAGGCAACGCCCTGGTTTGTACCTGCTATGGAGGAAGCCGGGGTTTTAACTGCGAGAGCAAGCCTGAGC CTGAAGAGACTTGCTTTGACAAATACACTGGGAACACTTACAAAGTGGGTGACACTTATGAGCGCCCTAA AGATTCCATGATCTGGGACTGTACCTGCATCGGGGCTGGGAGAGGCAGGATCAGCTGTACCATTGCAAAT CGCTGCCATGAAGGGGGTCAGTCCTACAAGATTGGCGACAAGTGGAGGAGGCCACATGAGACTGGTGGCT ACATGTTAGAGTGTCTGTGTCTGGGAAATGGAAAAGGGGAATGGACCTGCAAACCTATAGCTGAGAAGTG TTTTGATCATGCTGCTGGGACGTCCTACGTCGTGGGGGAGACCTGGGAAAAGCCCTACCAAGGCTGGATG ATGGTGGACTGTACTTGTCTAGGCGAAGGCAATGGACGCATCACCTGTACCTCCAGAAACAGATGCAACG ATCAGGACACCCGGACATCCTATAGGATTGGAGACACGTGGAGCAAGAAGGACAACCGAGGAAACCTGCT TCAGTGTGTCTGCACAGGCAATGGCAGAGGGGAGTGGAAGTGTGAGCGACATGCTCTACAAAGTGCTTCA GCCGGATCTGGCTCCTTCACTGATGTCCGAACAGCTATTTACCAACCGCAGACTCACCCCCAGCCCGCTC CCTACGGCCACTGTGTCACCGACAGTGGTGTGGTCTACTCTGTGGGAATGCAGTGGCTGAAGTCGCAAGG AAACAAGCAAATGCTGTGCACGTGCCTGGGCAATGGCGTCAGCTGCCAGGAGACAGCCGTGACCCAGACT TATGGTGGCAATTCAAACGGGGAGCCCTGTGTCCTCCCGTTCACCTACAACGGTAGGACCTTCTATTCCT GCACCACCGAAGGGCGGCAAGACGGACATCTGTGGTGTAGCACAACTTCCAATTACGAACAAGACCAGAA GTATTCCTTCTGCACAGACCATGCGGTTTTGGTTCAGACTCGAGGCGGAAATTCCAATGGTGCTCTGTGC CACTTCCCCTTCCTGTACAACAACCGGAATTACACCGACTGTACTTCTGAGGGTCGCAGGGACAACATGA AATGGTGCGGCACCACCCAGAACTACGATGCCGATCAGAAGTTTGGATTCTGCCCAATGGCTGCCCACGA GGAGATCTGCACAACCAATGAAGGGGTCATGTATCGCATTGGGGATCAGTGGGATAAGCAGCATGACCTG GGCCACATGATGAGGTGCACGTGTGTGGGGAACGGTCGTGGAGAATGGGCCTGCATCCCCTACTCCCAGC TCCGAGACCAGTGCATCGTTGATGACATTACTTACAATGTGAACGACACGTTCCACAAGCGTCACGAGGA GGGACATATGCTGAACTGTACCTGCTTTGGTCAGGGCCGGGGCAGATGGAAGTGTGACCCCATTGACCAG TGCCAAGATTCAGAGACCCGGACATTTTACCAGATTGGTGACTCCTGGGAGAAGTTTGTGCATGGTGTCC GATACCAGTGTTACTGCTACGGCCGTGGCATCGGGGAGTGGCACTGTCAACCTCTGCAGACCTACCCAGG CACAACTGGACCTGTCCAAGTAATTATCACGGAGACCCCCAGCCAGCCCAATTCCCACCCCATCCAGTGG AATGCCCCGGAGCCTTCACACATCACCAAGTACATTCTCAGATGGAGACCTAAAACCTCTACGGGTCGCT GGAAGGAAGCTACCATTCCTGGCCACCTTAACTCCTATACCATCAAAGGCCTGACCCCAGGTGTGATCTA TGAGGGACAGCTCATCAGCATCCAGCAGTATGGCCACAGAGAAGTGACACGCTTCGACTTCACCACCAGC GCCAGCACCCCTGTGACCAGCAACACGGTGACCGGAGAGACTGCGCCCTACTCTCCTGTTGTGGCCACTT CTGAATCTGTAACTGAAATCACAGCCAGCAGCTTTGTGGTCTCATGGGTCTCAGCCTCCGACACCGTGTC AGGCTTCCGGGTGGAGTATGAGCTGAGCGAGGAGGGAGATGAACCACAGTACCTTGATCTCCCAAGCACG GCCACTTCCGTGAACATTCCTGACCTGCTCCCGGGCAGAAAGTACATTGTCAATGTCTATCAGATATCTG AGGAGGGAAAACAGAGCTTGATCCTGTCTACCTCACAGACTACAGCACCTGACGCTCCTCCAGACCCTAC CGTGGACCAGGTTGATGATACTTCCATTGTTGTTCGGTGGAGTAGACCCCAGGCACCTATCACAGGGTAT AGAATTGTCTATTCACCTTCAGTAGAAGGCAGTAGCACAGAGCTCAACCTCCCTGAAACGGCCAACTCCG TCACCCTCAGCGACCTGCAGCCCGGTGTTCAGTACAACATCACTATCTATGCTGTGGAGGAGAACCAGGA GAGCACACCCGTTTTCATCCAACAAGAGACCACTGGCACCCCAAGATCTGATAACGTCCCCCCTCCGACG GACCTACAGTTTGTGGAACTGACTGATGTGAAAGTCACCATCATGTGGACCCCTCCTGATAGTGTGGTGT CTGGATACCGTGTGGAGGTCCTGCCTGTCAGCCTGCCCGGGGAACATGGGCAGAGGCTGCCTGTCAACAG AAATACCTTTGCTGAAATCACTGGGCTGTCCCCTGGGGTCACGTACCTCTTCAAAGTCTTTGCTGTGCAC CAGGGCAGGGAAAGCAATCCTCTGACGGCACAACAGACCACCAAACTCGACGCTCCCACTAACCTCCAGT TTGTCAATGAAACTGACAGAACAGTTCTGGTAACGTGGACTCCACCTCGAGCCCGTATAGCAGGCTACCG ACTGACCGCGGGCCTGACCCGAGGAGGCCAGCCCAAGCAGTACAATGTGGGACCCTTGGCCTCCAAGTAT CCCCTGAGAAATCTGCAGCCTGGGTCTGAGTACACCGTGACCTTGGTGGCTGTGAAAGGGAACCAGCAGA GTCCCAAAGCCACCGGAGTCTTTACTACCCTGCAGCCTCTGCGCTCCATTCCACCTTACAACACCGAGGT GACAGAGACCACAATTGTGATCACCTGGACCCCCGCTCCAAGGATTGGCTTCAAGCTGGGTGTACGACCG AGCCAGGGAGGTGAGGCACCCCGAGAAGTGACTTCAGACTCTGGGAGCATTGTTGTGTCTGGCTTGACTC CAGGCGTGGAATACACTTACACCATCCAAGTCCTGCGAGATGGCCAGGAGAGAGATGCACCGATTGTCAA CAGAGTAGTGACACCGCTGTCTCCACCGACCAACTTGCATCTGGAGGCAAACCCTGACACTGGAGTGCTT ACTGTCTCCTGGGAGAGGAGCACTACCCCAGATATCACTGGCTACAGAATAACTACTACCCCCACGAACG GGCAGCAGGGGACCTCTCTGGAAGAAGTGGTCCATGCTGATCAGAGTTCCTGCACTTTTGAGAACCTGAA TCCTGGCCTGGAGTACAACGTCAGTGTTTACACTGTCAAAGATGACAAGGAAAGTGCCCCTATCTCTGAT ACCGTTGTCCCAGAGGTGCCCCAGCTCACTGACCTAAGCTTTGTTGATATAACTGATTCAAGCATCGGCC TGAGGTGGACCCCGCTAAACTCTTCCACCATTATCGGGTACCGAATCACAGTAGTTGCGGCAGGAGAAGG GATCCCTATTTTTGAAGATTTTGTGGACTCCTCAGTAGGATACTACACAGTTACAGGGCTGGAGCCTGGC ATTGACTATGACATCAGCGTTATCACTCTCATTAATGGCGGAGAGAGTGCCCCTACTACACTGACACAGC AAACGGCTGTCCCTCCTCCCACGGATCTGCGATTCACCAATATCGGTCCAGACACGATGCGGGTCACTTG GGCCCCGCCTCCGTCCATCGAGCTGACCAACCTCTTGGTGCGCTACTCACCCGTGAAGAATGAAGAGGAC GTTGCAGAGCTATCCATTTCACCTTCAGACAATGCCGTGGTCCTAACAAATCTCCTGCCTGGGACAGAAT ACTTAGTCAGTGTCTCCAGTGTCTACGAACAACATGAGAGCATCCCTCTCCGGGGAAGGCAGAAAACAGG TCTCGATTCCCCAACTGGTTTTGATTCTTCTGATATCACCGCCAACTCATTCACTGTCCACTGGGTGGCT CCTCGGGCCCCCATCACCGGCTACATCATCCGCCATCACGCCGAGCATTCTGTCGGAAGACCCAGGCAGG ATCGAGTGCCGCCCTCGCGGAATTCCATCACCCTCACCAACCTTAATCCGGGCACCGAGTACGTTGTCAG CATCATTGCTGTTAATGGCAGAGAGGAGAGCCCGCCACTGATTGGCCAGCAAGCCACAGTTTCTGATATT CCGAGAGATCTGGAGGTCATTGCCTCCACCCCCACCAGCCTGCTCATCAGTTGGGAACCCCCTGCCGTCT CTGTGCGCTATTACAGAATCACCTACGGAGAGACAGGAGGAAATAGCCCTGTCCAGGAGTTCACTGTGCC CGGAAGCAAGTCCACAGCCACCATCAACAACATTAAACCAGGAGCAGACTACACCATCACCCTGTATGCT GTCACTGGCCGTGGGGACAGTCCAGCAAGCAGCAAGCCAGTTTCCATCAATTATAAAACAGAAATTGACA AGCCGTCCCAGATGCAGGTGACAGATGTCCAGGACAACAGCATCAGTGTCAGGTGGCTGCCTTCAACTTC TCCTGTGACAGGCTACAGAGTGACCACCACTCCCAAAAATGGCCTAGGACCATCAAAAACTAAAACTGCC AGTCCAGATCAAACAGAAATGACCATTGAAGGTTTGCAACCCACTGTGGAGTACGTGGTTAGTGTTTATG CTCAGAACCGGAACGGAGAAAGCCAGCCCCTGGTTCAAACTGCAGTGACCAACATTGATCGCCCTAAAGG ACTGGCATTCACTGATGTGGATGTCGATTCCATCAAAATTGCTTGGGAAAGCCCACAGGGGCAAGTTTCC AGGTACAGGGTGACCTACTCGAGCCCTGAGGATGGAATCCGGGAGCTTTTCCCTGCACCTGATGGTGAAG ACGACACTGCAGAGCTGCAGGGCCTCAGGCCGGGGTCTGAGTACACAGTCAGTGTGGTTGCCTTGCACGA TGATATGGAGAGCCAGCCCCTGATTGGAATCCAGTCCACAGCCATTCCTGCGCCCACCAATCTGAAGTTC AGTCAGGTGACACCCACCAGCTTTACTGCCCAGTGGATAGCACCCAGTGTTCAGCTCACTGGCTACCGGG TGCGGGTGAACCCGAAAGAGAAGACAGGACCAATGAAAGAAATCAACCTTTCTCCAGACAGCTCATCGGT GATTGTGTCAGGACTCATGGTGGCCACTAAATACGAAGTCAGTGTCTATGCTCTCAAGGACACACTGACA AGCAGACCAGCCCAGGGAGTCATCACTACTCTGGAGAATGTTAGCCCTCCAAGAAGGGCCCGTGTGACGG ACGCTACAGAGACCACCATCACTATTAGCTGGAGAACAAAGACAGAGACAATCACTGGCTTCCAAGTCGA TGCCATCCCAGCCAATGGCCAGACCCCAGTTCAGAGGAGCATCAGCCCGGATGTTAGAAGCTACACCATT ACAGGTTTACAGCCAGGCACTGACTACAAGATCCACCTGTACACTCTGAACGACAATGCCCGGAGCTCGC CCGTGATCATCGATGCCTCCACCGCCATCGACGCACCATCCAACCTGCGGTTCCTGACCACCACACCCAA CTCCTTGCTGGTGTCATGGCAGGCGCCCCGTGCCAGGATTACTGGCTACATTATCAAGTATGAGAAGCCT GGATCCCCTCCCAGAGAAGTGGTCCCTCGGCCCCGCCCTGGTGTCACGGAGGCCACCATTACTGGTCTGG AGCCAGGAACCGAGTACACCATCTACGTCATTGCCCTGAAGAACAATCAGAAGAGTGAGCCCCTGATTGG GAGGAAGAAGACAGATGAGCTTCCCCAACTGGTTACCCTTCCACACCCCAATCTTCATGGACCAGAGATC TTGGATGTTCCCTCCACAGTTCAAAAGACCCCCTTCATCACCAACCCTGGGTATGACACCGAAAATGGTA TTCAGCTTCCTGGCACAACCCACCAGCAACCCAGTGTTGGGCAACAAATGATCTTTGAGGAACATGGCTT TAGGCGGACAACGCCACCCACTGCGGCCACCCCCGTCAGGCTTAGGCCAAGACCATACCTGCCGAATGTA GATGAGGAGGTCCAAATCGGTCATGTTCCCAGGGGAGACGTAGACTACCACCTCTATCCTCATGTTCCGG GCCTCAATCCAAATGCCTCTACAGGACAGGAAGCTCTCTCTCAGACAACCATCTCTTGGACGCCGTTCCA GGAGAGTTCTGAGTACATCATTTCATGCCAACCAGTTGGCACCGACGAAGAGCCCTTACAGTTCCAAGTT CCTGGAACTTCTACCAGTGCGACTCTGACTGGCCTTACCAGAGGGGTCACCTACAACATCATAGTGGAGG CACTGCAGAACCAGAGGAGGCACAAGGTTCGGGAAGAGGTTGTGACTGTGGGCAACGCTGTCAGCGAAGG CCTGAACCAGCCTACAGATGACTCATGCTTTGACCCTTACACGGTTTCCCATTACGCCATTGGAGAGGAG TGGGAGCGGTTGTCTGACGCTGGCTTTAAGCTCACATGCCAGTGCTTGGGCTTTGGCAGTGGTCATTTCA GATGCGATTCATCTAAATGGTGCCATGACAACGGTGTCAACTACAAGATCGGAGAGAAGTGGGATCGGCA GGGAGAAAATGGCCAGCGGATGAGCTGCACATGCCTCGGGAATGGAAAGGGAGAATTCAAGTGTGATCCC CATGAAGCAACGTGCTATGACGATGGGAAGACCTACCATGTAGGAGAACAGTGGCAGAAAGAATATCTCG GAGCCATTTGCTCCTGCACGTGTTTCGGAGGCCAGCGGGGCTGGCGCTGTGACAACTGCCGTAGACCTGG GGCTGCTGAACCCAGTCCCGATGGCACCACCGGCCACACCTACAACCAGTATACACAGAGATACAATCAG AGAACAAACACTAACGTAAATTGCCCCATTGAGTGCTTCATGCCGCTAGATGTGCAAGCTGACAGAGACG ATTCTCGAGAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_010233
Insert Size	7434 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_010233.2, NP_034363.1
RefSeq Size	8425 bp
RefSeq ORF	7434 bp
Locus ID	14268
UniProt ID	P11276
Gene Summary	Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).

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