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Fn1 (NM_010233) Mouse Tagged ORF Clone
CAT＃: MG227059
- TrueORF®
Fn1 (tGFP-tagged) - Mouse fibronectin 1 (Fn1), (10ug)
ORF Plasmid: DDK
"NM_010233" in other vectors (2)
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CNY 21,280.00
货期*
3周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | E330027I09; Fn; Fn-1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG227059 representing NM_010233
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCAGGGGTCCGGGACCCGGGCGGCTGCTGCTGCTGGCAGTCCTGTGCCTGGGGACCTCGGTGCGCT GCACCGAAGCCGGGAAGAGCAAGAGGCAGGCTCAGCAAATCGTGCAGCCTCAATCCCCGGTGGCTGTCAG TCAGAGCAAGCCTGGCTGTTTTGACAATGGGAAGCACTATCAGATAAATCAGCAGTGGGAACGGACCTAC CTAGGCAACGCCCTGGTTTGTACCTGCTATGGAGGAAGCCGGGGTTTTAACTGCGAGAGCAAGCCTGAGC CTGAAGAGACTTGCTTTGACAAATACACTGGGAACACTTACAAAGTGGGTGACACTTATGAGCGCCCTAA AGATTCCATGATCTGGGACTGTACCTGCATCGGGGCTGGGAGAGGCAGGATCAGCTGTACCATTGCAAAT CGCTGCCATGAAGGGGGTCAGTCCTACAAGATTGGCGACAAGTGGAGGAGGCCACATGAGACTGGTGGCT ACATGTTAGAGTGTCTGTGTCTGGGAAATGGAAAAGGGGAATGGACCTGCAAACCTATAGCTGAGAAGTG TTTTGATCATGCTGCTGGGACGTCCTACGTCGTGGGGGAGACCTGGGAAAAGCCCTACCAAGGCTGGATG ATGGTGGACTGTACTTGTCTAGGCGAAGGCAATGGACGCATCACCTGTACCTCCAGAAACAGATGCAACG ATCAGGACACCCGGACATCCTATAGGATTGGAGACACGTGGAGCAAGAAGGACAACCGAGGAAACCTGCT TCAGTGTGTCTGCACAGGCAATGGCAGAGGGGAGTGGAAGTGTGAGCGACATGCTCTACAAAGTGCTTCA GCCGGATCTGGCTCCTTCACTGATGTCCGAACAGCTATTTACCAACCGCAGACTCACCCCCAGCCCGCTC CCTACGGCCACTGTGTCACCGACAGTGGTGTGGTCTACTCTGTGGGAATGCAGTGGCTGAAGTCGCAAGG AAACAAGCAAATGCTGTGCACGTGCCTGGGCAATGGCGTCAGCTGCCAGGAGACAGCCGTGACCCAGACT TATGGTGGCAATTCAAACGGGGAGCCCTGTGTCCTCCCGTTCACCTACAACGGTAGGACCTTCTATTCCT GCACCACCGAAGGGCGGCAAGACGGACATCTGTGGTGTAGCACAACTTCCAATTACGAACAAGACCAGAA GTATTCCTTCTGCACAGACCATGCGGTTTTGGTTCAGACTCGAGGCGGAAATTCCAATGGTGCTCTGTGC CACTTCCCCTTCCTGTACAACAACCGGAATTACACCGACTGTACTTCTGAGGGTCGCAGGGACAACATGA AATGGTGCGGCACCACCCAGAACTACGATGCCGATCAGAAGTTTGGATTCTGCCCAATGGCTGCCCACGA GGAGATCTGCACAACCAATGAAGGGGTCATGTATCGCATTGGGGATCAGTGGGATAAGCAGCATGACCTG GGCCACATGATGAGGTGCACGTGTGTGGGGAACGGTCGTGGAGAATGGGCCTGCATCCCCTACTCCCAGC TCCGAGACCAGTGCATCGTTGATGACATTACTTACAATGTGAACGACACGTTCCACAAGCGTCACGAGGA GGGACATATGCTGAACTGTACCTGCTTTGGTCAGGGCCGGGGCAGATGGAAGTGTGACCCCATTGACCAG TGCCAAGATTCAGAGACCCGGACATTTTACCAGATTGGTGACTCCTGGGAGAAGTTTGTGCATGGTGTCC GATACCAGTGTTACTGCTACGGCCGTGGCATCGGGGAGTGGCACTGTCAACCTCTGCAGACCTACCCAGG CACAACTGGACCTGTCCAAGTAATTATCACGGAGACCCCCAGCCAGCCCAATTCCCACCCCATCCAGTGG AATGCCCCGGAGCCTTCACACATCACCAAGTACATTCTCAGATGGAGACCTAAAACCTCTACGGGTCGCT GGAAGGAAGCTACCATTCCTGGCCACCTTAACTCCTATACCATCAAAGGCCTGACCCCAGGTGTGATCTA TGAGGGACAGCTCATCAGCATCCAGCAGTATGGCCACAGAGAAGTGACACGCTTCGACTTCACCACCAGC GCCAGCACCCCTGTGACCAGCAACACGGTGACCGGAGAGACTGCGCCCTACTCTCCTGTTGTGGCCACTT CTGAATCTGTAACTGAAATCACAGCCAGCAGCTTTGTGGTCTCATGGGTCTCAGCCTCCGACACCGTGTC AGGCTTCCGGGTGGAGTATGAGCTGAGCGAGGAGGGAGATGAACCACAGTACCTTGATCTCCCAAGCACG GCCACTTCCGTGAACATTCCTGACCTGCTCCCGGGCAGAAAGTACATTGTCAATGTCTATCAGATATCTG AGGAGGGAAAACAGAGCTTGATCCTGTCTACCTCACAGACTACAGCACCTGACGCTCCTCCAGACCCTAC CGTGGACCAGGTTGATGATACTTCCATTGTTGTTCGGTGGAGTAGACCCCAGGCACCTATCACAGGGTAT AGAATTGTCTATTCACCTTCAGTAGAAGGCAGTAGCACAGAGCTCAACCTCCCTGAAACGGCCAACTCCG TCACCCTCAGCGACCTGCAGCCCGGTGTTCAGTACAACATCACTATCTATGCTGTGGAGGAGAACCAGGA GAGCACACCCGTTTTCATCCAACAAGAGACCACTGGCACCCCAAGATCTGATAACGTCCCCCCTCCGACG GACCTACAGTTTGTGGAACTGACTGATGTGAAAGTCACCATCATGTGGACCCCTCCTGATAGTGTGGTGT CTGGATACCGTGTGGAGGTCCTGCCTGTCAGCCTGCCCGGGGAACATGGGCAGAGGCTGCCTGTCAACAG AAATACCTTTGCTGAAATCACTGGGCTGTCCCCTGGGGTCACGTACCTCTTCAAAGTCTTTGCTGTGCAC CAGGGCAGGGAAAGCAATCCTCTGACGGCACAACAGACCACCAAACTCGACGCTCCCACTAACCTCCAGT TTGTCAATGAAACTGACAGAACAGTTCTGGTAACGTGGACTCCACCTCGAGCCCGTATAGCAGGCTACCG ACTGACCGCGGGCCTGACCCGAGGAGGCCAGCCCAAGCAGTACAATGTGGGACCCTTGGCCTCCAAGTAT CCCCTGAGAAATCTGCAGCCTGGGTCTGAGTACACCGTGACCTTGGTGGCTGTGAAAGGGAACCAGCAGA GTCCCAAAGCCACCGGAGTCTTTACTACCCTGCAGCCTCTGCGCTCCATTCCACCTTACAACACCGAGGT GACAGAGACCACAATTGTGATCACCTGGACCCCCGCTCCAAGGATTGGCTTCAAGCTGGGTGTACGACCG AGCCAGGGAGGTGAGGCACCCCGAGAAGTGACTTCAGACTCTGGGAGCATTGTTGTGTCTGGCTTGACTC CAGGCGTGGAATACACTTACACCATCCAAGTCCTGCGAGATGGCCAGGAGAGAGATGCACCGATTGTCAA CAGAGTAGTGACACCGCTGTCTCCACCGACCAACTTGCATCTGGAGGCAAACCCTGACACTGGAGTGCTT ACTGTCTCCTGGGAGAGGAGCACTACCCCAGATATCACTGGCTACAGAATAACTACTACCCCCACGAACG GGCAGCAGGGGACCTCTCTGGAAGAAGTGGTCCATGCTGATCAGAGTTCCTGCACTTTTGAGAACCTGAA TCCTGGCCTGGAGTACAACGTCAGTGTTTACACTGTCAAAGATGACAAGGAAAGTGCCCCTATCTCTGAT ACCGTTGTCCCAGAGGTGCCCCAGCTCACTGACCTAAGCTTTGTTGATATAACTGATTCAAGCATCGGCC TGAGGTGGACCCCGCTAAACTCTTCCACCATTATCGGGTACCGAATCACAGTAGTTGCGGCAGGAGAAGG GATCCCTATTTTTGAAGATTTTGTGGACTCCTCAGTAGGATACTACACAGTTACAGGGCTGGAGCCTGGC ATTGACTATGACATCAGCGTTATCACTCTCATTAATGGCGGAGAGAGTGCCCCTACTACACTGACACAGC AAACGGCTGTCCCTCCTCCCACGGATCTGCGATTCACCAATATCGGTCCAGACACGATGCGGGTCACTTG GGCCCCGCCTCCGTCCATCGAGCTGACCAACCTCTTGGTGCGCTACTCACCCGTGAAGAATGAAGAGGAC GTTGCAGAGCTATCCATTTCACCTTCAGACAATGCCGTGGTCCTAACAAATCTCCTGCCTGGGACAGAAT ACTTAGTCAGTGTCTCCAGTGTCTACGAACAACATGAGAGCATCCCTCTCCGGGGAAGGCAGAAAACAGG TCTCGATTCCCCAACTGGTTTTGATTCTTCTGATATCACCGCCAACTCATTCACTGTCCACTGGGTGGCT CCTCGGGCCCCCATCACCGGCTACATCATCCGCCATCACGCCGAGCATTCTGTCGGAAGACCCAGGCAGG ATCGAGTGCCGCCCTCGCGGAATTCCATCACCCTCACCAACCTTAATCCGGGCACCGAGTACGTTGTCAG CATCATTGCTGTTAATGGCAGAGAGGAGAGCCCGCCACTGATTGGCCAGCAAGCCACAGTTTCTGATATT CCGAGAGATCTGGAGGTCATTGCCTCCACCCCCACCAGCCTGCTCATCAGTTGGGAACCCCCTGCCGTCT CTGTGCGCTATTACAGAATCACCTACGGAGAGACAGGAGGAAATAGCCCTGTCCAGGAGTTCACTGTGCC CGGAAGCAAGTCCACAGCCACCATCAACAACATTAAACCAGGAGCAGACTACACCATCACCCTGTATGCT GTCACTGGCCGTGGGGACAGTCCAGCAAGCAGCAAGCCAGTTTCCATCAATTATAAAACAGAAATTGACA AGCCGTCCCAGATGCAGGTGACAGATGTCCAGGACAACAGCATCAGTGTCAGGTGGCTGCCTTCAACTTC TCCTGTGACAGGCTACAGAGTGACCACCACTCCCAAAAATGGCCTAGGACCATCAAAAACTAAAACTGCC AGTCCAGATCAAACAGAAATGACCATTGAAGGTTTGCAACCCACTGTGGAGTACGTGGTTAGTGTTTATG CTCAGAACCGGAACGGAGAAAGCCAGCCCCTGGTTCAAACTGCAGTGACCAACATTGATCGCCCTAAAGG ACTGGCATTCACTGATGTGGATGTCGATTCCATCAAAATTGCTTGGGAAAGCCCACAGGGGCAAGTTTCC AGGTACAGGGTGACCTACTCGAGCCCTGAGGATGGAATCCGGGAGCTTTTCCCTGCACCTGATGGTGAAG ACGACACTGCAGAGCTGCAGGGCCTCAGGCCGGGGTCTGAGTACACAGTCAGTGTGGTTGCCTTGCACGA TGATATGGAGAGCCAGCCCCTGATTGGAATCCAGTCCACAGCCATTCCTGCGCCCACCAATCTGAAGTTC AGTCAGGTGACACCCACCAGCTTTACTGCCCAGTGGATAGCACCCAGTGTTCAGCTCACTGGCTACCGGG TGCGGGTGAACCCGAAAGAGAAGACAGGACCAATGAAAGAAATCAACCTTTCTCCAGACAGCTCATCGGT GATTGTGTCAGGACTCATGGTGGCCACTAAATACGAAGTCAGTGTCTATGCTCTCAAGGACACACTGACA AGCAGACCAGCCCAGGGAGTCATCACTACTCTGGAGAATGTTAGCCCTCCAAGAAGGGCCCGTGTGACGG ACGCTACAGAGACCACCATCACTATTAGCTGGAGAACAAAGACAGAGACAATCACTGGCTTCCAAGTCGA TGCCATCCCAGCCAATGGCCAGACCCCAGTTCAGAGGAGCATCAGCCCGGATGTTAGAAGCTACACCATT ACAGGTTTACAGCCAGGCACTGACTACAAGATCCACCTGTACACTCTGAACGACAATGCCCGGAGCTCGC CCGTGATCATCGATGCCTCCACCGCCATCGACGCACCATCCAACCTGCGGTTCCTGACCACCACACCCAA CTCCTTGCTGGTGTCATGGCAGGCGCCCCGTGCCAGGATTACTGGCTACATTATCAAGTATGAGAAGCCT GGATCCCCTCCCAGAGAAGTGGTCCCTCGGCCCCGCCCTGGTGTCACGGAGGCCACCATTACTGGTCTGG AGCCAGGAACCGAGTACACCATCTACGTCATTGCCCTGAAGAACAATCAGAAGAGTGAGCCCCTGATTGG GAGGAAGAAGACAGATGAGCTTCCCCAACTGGTTACCCTTCCACACCCCAATCTTCATGGACCAGAGATC TTGGATGTTCCCTCCACAGTTCAAAAGACCCCCTTCATCACCAACCCTGGGTATGACACCGAAAATGGTA TTCAGCTTCCTGGCACAACCCACCAGCAACCCAGTGTTGGGCAACAAATGATCTTTGAGGAACATGGCTT TAGGCGGACAACGCCACCCACTGCGGCCACCCCCGTCAGGCTTAGGCCAAGACCATACCTGCCGAATGTA GATGAGGAGGTCCAAATCGGTCATGTTCCCAGGGGAGACGTAGACTACCACCTCTATCCTCATGTTCCGG GCCTCAATCCAAATGCCTCTACAGGACAGGAAGCTCTCTCTCAGACAACCATCTCTTGGACGCCGTTCCA GGAGAGTTCTGAGTACATCATTTCATGCCAACCAGTTGGCACCGACGAAGAGCCCTTACAGTTCCAAGTT CCTGGAACTTCTACCAGTGCGACTCTGACTGGCCTTACCAGAGGGGTCACCTACAACATCATAGTGGAGG CACTGCAGAACCAGAGGAGGCACAAGGTTCGGGAAGAGGTTGTGACTGTGGGCAACGCTGTCAGCGAAGG CCTGAACCAGCCTACAGATGACTCATGCTTTGACCCTTACACGGTTTCCCATTACGCCATTGGAGAGGAG TGGGAGCGGTTGTCTGACGCTGGCTTTAAGCTCACATGCCAGTGCTTGGGCTTTGGCAGTGGTCATTTCA GATGCGATTCATCTAAATGGTGCCATGACAACGGTGTCAACTACAAGATCGGAGAGAAGTGGGATCGGCA GGGAGAAAATGGCCAGCGGATGAGCTGCACATGCCTCGGGAATGGAAAGGGAGAATTCAAGTGTGATCCC CATGAAGCAACGTGCTATGACGATGGGAAGACCTACCATGTAGGAGAACAGTGGCAGAAAGAATATCTCG GAGCCATTTGCTCCTGCACGTGTTTCGGAGGCCAGCGGGGCTGGCGCTGTGACAACTGCCGTAGACCTGG GGCTGCTGAACCCAGTCCCGATGGCACCACCGGCCACACCTACAACCAGTATACACAGAGATACAATCAG AGAACAAACACTAACGTAAATTGCCCCATTGAGTGCTTCATGCCGCTAGATGTGCAAGCTGACAGAGACG ATTCTCGAGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG227059 representing NM_010233
Red=Cloning site Green=Tags(s) MLRGPGPGRLLLLAVLCLGTSVRCTEAGKSKRQAQQIVQPQSPVAVSQSKPGCFDNGKHYQINQQWERTY LGNALVCTCYGGSRGFNCESKPEPEETCFDKYTGNTYKVGDTYERPKDSMIWDCTCIGAGRGRISCTIAN RCHEGGQSYKIGDKWRRPHETGGYMLECLCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWM MVDCTCLGEGNGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCVCTGNGRGEWKCERHALQSAS AGSGSFTDVRTAIYQPQTHPQPAPYGHCVTDSGVVYSVGMQWLKSQGNKQMLCTCLGNGVSCQETAVTQT YGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDHAVLVQTRGGNSNGALC HFPFLYNNRNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRIGDQWDKQHDL GHMMRCTCVGNGRGEWACIPYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPIDQ CQDSETRTFYQIGDSWEKFVHGVRYQCYCYGRGIGEWHCQPLQTYPGTTGPVQVIITETPSQPNSHPIQW NAPEPSHITKYILRWRPKTSTGRWKEATIPGHLNSYTIKGLTPGVIYEGQLISIQQYGHREVTRFDFTTS ASTPVTSNTVTGETAPYSPVVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPST ATSVNIPDLLPGRKYIVNVYQISEEGKQSLILSTSQTTAPDAPPDPTVDQVDDTSIVVRWSRPQAPITGY RIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVFIQQETTGTPRSDNVPPPT DLQFVELTDVKVTIMWTPPDSVVSGYRVEVLPVSLPGEHGQRLPVNRNTFAEITGLSPGVTYLFKVFAVH QGRESNPLTAQQTTKLDAPTNLQFVNETDRTVLVTWTPPRARIAGYRLTAGLTRGGQPKQYNVGPLASKY PLRNLQPGSEYTVTLVAVKGNQQSPKATGVFTTLQPLRSIPPYNTEVTETTIVITWTPAPRIGFKLGVRP SQGGEAPREVTSDSGSIVVSGLTPGVEYTYTIQVLRDGQERDAPIVNRVVTPLSPPTNLHLEANPDTGVL TVSWERSTTPDITGYRITTTPTNGQQGTSLEEVVHADQSSCTFENLNPGLEYNVSVYTVKDDKESAPISD TVVPEVPQLTDLSFVDITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIPIFEDFVDSSVGYYTVTGLEPG IDYDISVITLINGGESAPTTLTQQTAVPPPTDLRFTNIGPDTMRVTWAPPPSIELTNLLVRYSPVKNEED VAELSISPSDNAVVLTNLLPGTEYLVSVSSVYEQHESIPLRGRQKTGLDSPTGFDSSDITANSFTVHWVA PRAPITGYIIRHHAEHSVGRPRQDRVPPSRNSITLTNLNPGTEYVVSIIAVNGREESPPLIGQQATVSDI PRDLEVIASTPTSLLISWEPPAVSVRYYRITYGETGGNSPVQEFTVPGSKSTATINNIKPGADYTITLYA VTGRGDSPASSKPVSINYKTEIDKPSQMQVTDVQDNSISVRWLPSTSPVTGYRVTTTPKNGLGPSKTKTA SPDQTEMTIEGLQPTVEYVVSVYAQNRNGESQPLVQTAVTNIDRPKGLAFTDVDVDSIKIAWESPQGQVS RYRVTYSSPEDGIRELFPAPDGEDDTAELQGLRPGSEYTVSVVALHDDMESQPLIGIQSTAIPAPTNLKF SQVTPTSFTAQWIAPSVQLTGYRVRVNPKEKTGPMKEINLSPDSSSVIVSGLMVATKYEVSVYALKDTLT SRPAQGVITTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAIPANGQTPVQRSISPDVRSYTI TGLQPGTDYKIHLYTLNDNARSSPVIIDASTAIDAPSNLRFLTTTPNSLLVSWQAPRARITGYIIKYEKP GSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTDELPQLVTLPHPNLHGPEI LDVPSTVQKTPFITNPGYDTENGIQLPGTTHQQPSVGQQMIFEEHGFRRTTPPTAATPVRLRPRPYLPNV DEEVQIGHVPRGDVDYHLYPHVPGLNPNASTGQEALSQTTISWTPFQESSEYIISCQPVGTDEEPLQFQV PGTSTSATLTGLTRGVTYNIIVEALQNQRRHKVREEVVTVGNAVSEGLNQPTDDSCFDPYTVSHYAIGEE WERLSDAGFKLTCQCLGFGSGHFRCDSSKWCHDNGVNYKIGEKWDRQGENGQRMSCTCLGNGKGEFKCDP HEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGAAEPSPDGTTGHTYNQYTQRYNQ RTNTNVNCPIECFMPLDVQADRDDSRE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010233 |
| ORF Size | 7431 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010233.2, NP_034363.1 |
| RefSeq Size | 8312 bp |
| RefSeq ORF | 7434 bp |
| Locus ID | 14268 |
| UniProt ID | P11276 |
| Gene Summary | Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.[UniProtKB/Swiss-Prot Function] |
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