Arid1b (NM_001085355) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	8030481M12; 9330189K18Rik; AI836955; Ardi1b; B230217J03Rik; mKIAA1235
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225243 representing NM_001085355 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACCGGGCTGCTCCCCAACCACAAACTGAAAGCCGTTGGCGAGGCCCCCGCTGCACCGCCCCATC AGCAGCACCACCACCACCATGCCCACCACCACCACCACCACCATGCCCACCACCTCCACCACCTCCACCA CCACCACGCACTACAGCAGCAGCTAAACCAGTTCCAGCAGCCGCAGCCGCCGCAGCCACAGCAGCAGCAG CCGCCGCCACCGCCGCAGCAGCAGCATCCCACTGCCAACAACAGCCTGGGCGGTGCGGGCGGCGGCGCGC CTCAGCCCGGCCCGGACATGGAGCAGCCGCAACATGGAGGCGCCAAGGACAGTGTCGCGGGCAATCAGGC TGACCCGCAGGGCCAGCCTCTGCTGAGCAAACCGGGCGACGAGGACGACGCGCCGCCCAAGATGGGGGAG CCGGCGGGCAGCCGCTATGAGCACCCGGGCCTGGGCGCGCAGCAGCAGCCCGCGCCGGTCGCCGTGCCCG GGGGCGGCGGCGGCCCAGCGGCCGTCTCGGAGTTTAATAATTACTATGGCAGCGCTGCCCCTGCTAGCGG CGGCCCCGGCGGCCGCGCTGGGCCTTGCTTTGATCAACATGGCGGACAACAAAGCCCCGGGATGGGGATG ATGCACTCCGCCTCTGCCGCCGCCGGGGCCCCCAGCAGCATGGACCCCCTGCAGAACTCCCACGAAGGGT ACCCCAACAGCCAGTACAACCATTATCCGGGCTACAGCCGGCCCGGCGCGGGCGGCGGCGGCGGCGGCGG CGGAGGAGGAGGAGGCAGCGGAGGAGGTGGAGGAGGAGGAGGAGCAGGAGGAGCAGGAGGAGCAGCGGCA GCGGCAGCAGGAGCCGGAGCTGTGGCGGCGGCGGCCGCGGCGGCGGCGGCAGCAGCAGCAGCAGCAGGAG GAGGCGGTGGCGGCGGCTATGGGAGCTCGTCCTCGGGGTACGGGGTGCTGAGCTCCCCGCGGCAGCAGGG CGGCGGCATGATGATGGGCCCCGGGGGCGGCGGGGCCGCGAGCCTCAGCAAGGCGGCCGCCGGCGCGGCG GCGGCGGCGGGGGGCTTCCAGCGCTTCGCCGGCCAGAACCAGCACCCGTCGGGGGCTACACCGACCCTCA ACCAGCTGCTCACCTCACCCAGCCCCATGATGAGGAGCTACGGCGGTAGCTACCCCGACTACAGCAGCTC CAGCGCGCCGCCGCCGCCGTCGCAGCCCCAGTCCCAGGCGGCGGCGGGGGCGGCGGCGGGTGGCCAGCAG GCGGCCGCGGGCATGGGCTTGGGCAAGGACCTAGGCGCCCAGTACGCCGCTGCCAGCCCGGCCTGGGCGG CCGCGCAACAAAGGAGTCACCCGGCGATGAGCCCCGGCACCCCCGGACCGACCATGGGCAGATCCCAGGG CAGCCCGATGGACCCAATGGTGATGAAGAGACCTCAGTTGTATGGGATGGGTACTCACCCCCACTCCCAG CCACAGCAGAGCAGCCCATACCCAGGAGGCTCCTACGGTCCCCCAGGTGCACAGCGGTATCCCCTTGGCA TGCAGGGCCGGGCTCCAGGGGCCCTGGGAGGCTTGCAGTACCCGCAGCAGCAGATGCCACCGCAGTACGG ACAGCAAGCTGTGAGTGGCTACTGCCAGCAAGGCCAGCAGCCATACTACAACCAGCAGCCGCAGCCCTCG CACCTCCCGCCCCAGGCACAGTACCTGCAGCCGGCGGCGGCGCAGTCCCAGCAGAGGTACCAGCCACAGC AGGACATGTCTCAAGAAGGCTATGGAACTAGATCTCAGCCTCCTCTGGCCCCTGGAAAATCCAACCATGA AGACTTGAATTTAATTCAACAGGAAAGACCATCGAGTCTACCAGACCTGTCTGGCTCCATCGATGACCTC CCCACGGGAACAGAAGCAACTCTGAGCTCAGCAGTCAGTGCATCCGGGTCTACAAGCAGCCAGGGAGATC AGAGCAACCCAGCGCAGTCTCCTTTCTCCCCACATGCATCACCTCACCTCTCCAGCATCCCTGGAGGGCC GTCACCTTCTCCTGTTGGCTCTCCTGTGGGAAGCAACCAATCGAGGTCTGGTCCGATCTCCCCTGCGAGT ATTCCAGGTAGCCAGATGCCTCCGCAACCACCTGGAAGCCAGTCAGAATCCAGTTCCCATCCTGCCTTGA GCCAGTCACCAATGCCACAGGAAAGAGGTTTTATGACAGGCACTCAGAGAAACCCTCAGATGTCTCAGTA CGGACCTCAGCAGACAGGACCATCCATGTCGCCTCACCCATCTCCTGGGGGCCAGATGCATCCTGGGATC AGTAACTTTCAGCAGAGTAACTCAAGTGGCACGTACGGCCCACAGATGAGCCAGTATGGACCCCAAGGCA ACTACTCCAGAACCCCAACATATAGCGGGGTACCCAGTGCAAGCTACAGCGGCCCAGGGCCCGGTATGGG CATCAATGCCAACAACCAGATGCATGGACAAGGGCCAGCCCAGCCATGTGGTGCTATGCCCCTGGGACGA ATGCCTTCAGCTGGGATGCAGAACAGACCATTTCCTGGAACCATGAGCAGCGTCACCCCCAGTTCTCCTG GCATGTCTCAACAGGGAGGGCCAGGAATGGGCCCACCAATGCCCACTGTGAACCGGAAGGCCCAGGAAGC TGCCGCAGCTGTGATGCAGGCTGCTGCAAACTCAGCACAAAGCAGGCAAGGCAGTTTTCCTGGCATGAAC CAGAGTGGCCTGGTGGCCTCCAGCTCTCCCTACAGCCAGTCCATGAACAACAACTCCAGCCTGATGAGCA CCCAGGCCCAGCCCTACAGCATGACGCCCACAATGGTGAACAGCTCCACAGCATCTATGGGTCTTGCAGA TATGATGTCTCCCAGTGAGTCCAAATTGTCTGTGCCTCTTAAAGCAGATGGTAAAGAAGAAGGCGTGTCC CAGCCTGAGAGCAAGTCAAAGGACAGCTATGGCTCTCAGGGCATTTCCCAGCCTCCAACCCCAGGCAACC TGCCTGTCCCTTCCCCAATGTCTCCCAGCTCTGCCAGCATCTCCTCCTTTCATGGAGATGAGAGTGACAG CATTAGCAGCCCAGGCTGGCCCAAGACACCATCAAGCCCTAAGTCCAGCTCTTCCTCCACCACTGGGGAG AAGATCACGAAGGTCTATGAGCTGGGGAATGAGCCGGAGAGGAAGCTGTGGGTCGACCGTTACCTAACGT TCATGGAAGAGAGGGGCTCCCCGGTGTCCAGTCTGCCAGCAGTGGGCAAGAAGCCCCTGGACCTGTTCCG ACTGTATGTCTGCGTCAAGGAGATTGGAGGTTTGGCGCAGGTTAATAAAAACAAGAAGTGGCGTGAGCTG GCAACCAACCTGAACGTTGGCACTTCCAGCAGCGCAGCCAGCTCTCTGAAAAAGCAGTATATTCAGTACC TGTTCGCCTTTGAGTGCAAAACTGAGCGCGGGGAGGAGCCCCCACCTGAAGTCTTCAGCACCGGGGATTC GAAGAAGCAGCCAAAGCTCCAGCCGCCATCTCCTGCTAACTCAGGATCCTTACAAGGCCCACAGACTCCA CAGTCAACTGGGAGCAATTCGATGGCAGAGGTTCCAGGTGACCTGAAGCCACCAACCCCAGCCTCTACCC CTCATGGACAGATGACTCCCATGCAAAGCGGAAGAAGCAGTACAGTCAGTGTGCATGACCCGTTCTCAGA CGTGAGTGACTCAGCGTACCCAAAACGGAACTCCATGACTCCAAACGCCCCATACCAGCAGGGCATGGGC ATGCCAGACATGATGGGCAGGATGCCCTATGAACCCAACAAGGACCCTTTCAGTGGAATGAGAAAAGTGC CTGGAAGTAGTGAGCCCTTTATGACACAAGGACAGGTGCCCAACAGCGGCATGCAGGACATGTACAACCA GAGCCCCTCAGGGGCCATGTCCAATCTGGGCATGGGACAGCGGCAGCAGTTTCCCTATGGAACCAGTTAT GACCGAAGGCATGAGGCTTACGGACAGCAGTACCCAGGCCAAGGCCCTCCCACAGGACAGCCACCGTATG GAGGACACCAGCCTGGCCTGTACCCACAGCAGCCGAATTACAAACGTCATATGGATGGCATGTACGGGCC TCCAGCCAAGCGGCACGAGGGAGACATGTACAACATGCAGTATGGCAGCCAGCAGCAGGAGATGTATAAC CAGTATGGAGGCTCCTACTCTGGCCCGGACAGAAGGCCCATCCAGGGACAATATCCCTACCCCTACAACA GAGAAAGGATGCAGGGCCCAGGCCAGATGCAGCCACACGGAATCCCACCTCAGATGATGGGGGGCCCCAT GCAGTCATCCTCCAGCGAGGGGCCTCAGCAGAACATGTGGGCTACACGCAACGATATGCCTTATCCCTAC CAGAGCAGGCAAGGCCCGGGCGGCCCTGCACAGGCCCCCCCTTACCCAGGCATGAACCGCACAGATGATA TGATGGTACCTGAGCAGAGGATCAATCACGAGAGCCAGTGGCCTTCTCACGTCAGCCAGCGCCAGCCTTA CATGTCATCTTCGGCCTCCATGCAGCCCATCACGCGCCCACCTCAGTCATCCTACCAGACGCCGCCGTCA CTGCCAAACCACATCTCCAGGGCACCCAGCCCCGCCTCCTTCCAGCGCTCCCTGGAGAGTCGCATGTCTC CAAGCAAGTCTCCCTTCCTGCCCACCATGAAGATGCAGAAGGTCATGCCCACAGTCCCCACATCCCAGGT CACCGGGCCCCCCCCACAGCCTCCACCAATCAGAAGGGAGATTACCTTTCCTCCTGGCTCCGTAGAAGCA TCACAGCCAATCCTGAAACAAAGGCGAAAGATTACCTCAAAAGATATTGTTACTCCCGAGGCGTGGCGTG TGATGATGTCCCTTAAATCGGGTCTGTTGGCTGAGAGCACGTGGGCTCTGGACACCATCAATATTCTCCT CTATGATGACAGCACCGTCGCCACCTTCAATCTTTCCCAGCTGTCTGGATTCCTGGAACTATTAGTAGAG TACTTTCGAAAATGCCTAATTGACATTTTCGGAATTCTTATGGAATATGAAGTGGGTGACCCCAGCCAAA AGGCTCTTGATCACCGTTCAGGGAAGAAAGATGACAGCCAGTCCCTGGAAGATGATTCTGGGAAGGAAGA CGATGATGCTGAGTGTCTTGTGGAAGAGGAGGAGGAGGAAGAGGAGGAGGAGGAAGACAGTGAAAAGATA GAGTCAGAGGGGAAGAGCAGCCCTGCCCTAGCTGCTCCAGATGCCTCCGTGGACCCCAAGGAGACGCCAA AGCAGGCCAGTAAGTTTGACAAGCTGCCCATAAAGATTGTCAAAAAGAACAAGCTGTTTGTGGTGGACCG GTCCGACAAGCTGGGCCGAGTGCAGGAGTTCAGCAGCGGGCTCCTCCACTGGCAGCTGGGTGGTGGCGAC ACTACCGAGCACATCCAGACTCACTTCGAGAGCAAGATGGAGATCCCTCCTCGCAGGCGTCCACCTCCGC CTCTAAGCTCCACGGGTAAGAAGAAAGAGCTGGAAGGCAAAGGTGATTCTGAAGAGCAGCCAGAGAAAAG TATCATAGCCACCATCGATGACGTCTTGTCTGCCCGGCCAGGGGCTCTGCCTGAAGACACCAACCCAGGA CCCCAGACCGACAGCGGCAAGTTTCCCTTTGGAATCCAGCAGGCCAAAAGCCACCGGAACATCAGGCTCC TGGAAGACGAGCCCAGGAGCCGAGACGAGACGCCGCTGTGCACCATCGCGCACTGGCAGGACTCACTGGC CAAGCGCTGCATCTGTGTGTCGAACATCGTGCGGAGCTTGTCTTTCGTGCCTGGCAACGACGCAGAGATG TCCAAACACCCGGGCTTGGTGCTGATCCTGGGAAAGCTGATTCTGCTGCATCACGAGCATCCGGAGAGAA AGCGGGCGCCACAGACCTATGAGAAGGAGGAGGACGAGGACAAGGGGGTGGCCTGCAGCAAAGATGAGTG GTGGTGGGACTGCCTCGAGGTCTTGCGGGATAACACCCTGGTCACGTTGGCGAACATTTCCGGGCAGCTA GACTTGTCTGCTTACACAGAGAGCATCTGCTTGCCGATCCTGGACGGCTTGCTACACTGGATGGTGTGCC CGTCCGCAGAGGCTCAGGACCCCTTTCCCACTGTGGGGCCCAACTCAGTCCTGTCGCCGCAGAGACTTGT GCTGGAGACCCTGTGTAAACTCAGTATCCAGGACAACAACGTGGACCTGATCTTGGCCACGCCTCCATTT AGTCGTCAGGAGAAATTTTATGCTACATTAGTTAGGTACGTTGGGGATCGCAAAAATCCAGTCTGTCGAG AAATGTCCATGGCGCTTTTATCGAACCTTGCCCAGGGGGACACACTGGCGGCGAGGGCAATAGCTGTGCA GAAAGGAAGCATTGGTAACTTGATAAGCTTCCTAGAGGACGGGGTGACGATGGCGCAGTACCAGCAGAGC CAGCATAACCTTATGCACATGCAGCCCCCACCTCTGGAACCCCCTAGTGTAGACATGATGTGCCGGGCGG CCAAAGCTCTGCTGGCCATGGCCAGAGTGGACGAGAACCGCTCGGAGTTCCTTTTGCACGAGGGTCGGTT GCTGGATATCTCAATATCAGCTGTCCTGAACTCTCTGGTTGCATCTGTCATCTGTGATGTACTGTTTCAG ATTGGGCAGTTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001085355
Insert Size	6735 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001085355.1, NP_001078824.1
RefSeq Size	11325 bp
RefSeq ORF	6735 bp
Locus ID	239985
UniProt ID	E9Q4N7
Gene Summary	Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (PubMed:17640523). Binds DNA non-specifically.[UniProtKB/Swiss-Prot Function]

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