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Arid1b (NM_001085355) Mouse Tagged ORF Clone
CAT＃: MG214151
- TrueORF®
Arid1b (tGFP-tagged) - Mouse AT rich interactive domain 1B (SWI-like) (Arid1b), (10ug). Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_001085355" in other vectors (2)
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CNY 27,080.00
货期*
3周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 8030481M12; 9330189K18Rik; AI836955; Ardi1b; B230217J03Rik; mKIAA1235 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG214151 representing NM_001085355, codon optimized.
Due to the complexity of NM_001085355, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACTGGCCTGCTGCCTAACCACAAGCTGAAAGCCGTGGGCGAGGCCCCAGCAGCCCCCCCGCATC AGCAGCATCACCACCATCATGCCCACCACCACCATCACCATCACGCTCACCATCTCCACCACCTGCATCA CCACCACGCTCTGCAACAGCAGCTGAACCAATTCCAGCAGCCACAGCCTCCGCAACCTCAGCAGCAGCAG CCCCCCCCTCCCCCTCAGCAGCAGCACCCCACAGCCAACAATAGTCTGGGCGGAGCAGGTGGAGGTGCCC CTCAACCCGGTCCAGACATGGAGCAGCCACAGCATGGTGGCGCAAAAGATTCTGTGGCTGGCAACCAGGC AGATCCTCAGGGTCAGCCACTGCTCAGCAAGCCGGGAGATGAGGATGACGCACCACCGAAGATGGGCGAA CCTGCCGGATCCCGCTACGAGCACCCAGGCCTCGGCGCCCAGCAGCAGCCCGCGCCTGTAGCTGTGCCAG GGGGGGGAGGTGGCCCTGCCGCTGTTTCCGAGTTCAACAATTATTATGGGTCCGCTGCACCCGCATCCGG GGGCCCGGGGGGCCGCGCTGGTCCATGCTTCGATCAGCATGGAGGCCAGCAGTCACCTGGCATGGGAATG ATGCACAGTGCATCTGCTGCAGCGGGCGCTCCTTCAAGCATGGATCCCCTCCAAAACTCCCATGAAGGAT ACCCTAATAGCCAGTATAACCACTATCCAGGATACAGTAGGCCAGGAGCCGGAGGAGGCGGTGGAGGCGG CGGGGGAGGCGGTGGATCTGGCGGTGGAGGCGGCGGTGGTGGAGCCGGCGGGGCCGGCGGCGCAGCGGCT GCTGCTGCTGGCGCCGGAGCTGTGGCCGCAGCAGCGGCTGCCGCCGCTGCTGCAGCCGCAGCTGCTGGCG GAGGGGGTGGCGGCGGCTATGGATCATCTTCATCTGGCTATGGGGTTCTCTCTTCTCCTAGGCAGCAAGG CGGAGGGATGATGATGGGGCCGGGCGGAGGGGGGGCAGCGTCCCTGAGCAAGGCAGCTGCCGGGGCCGCA GCTGCTGCAGGCGGTTTCCAGCGGTTTGCTGGTCAGAATCAGCATCCGAGCGGAGCCACCCCGACTCTGA ATCAGCTCCTCACTTCTCCTAGCCCTATGATGCGCAGCTACGGTGGTTCTTACCCCGACTACAGTTCCTC ATCTGCCCCACCTCCACCTAGCCAACCACAGAGTCAGGCCGCAGCTGGAGCGGCCGCTGGCGGACAGCAG GCGGCCGCTGGCATGGGCCTGGGTAAGGACTTGGGTGCCCAGTACGCCGCTGCTTCTCCAGCATGGGCCG CAGCCCAGCAGCGGTCACACCCTGCGATGTCCCCAGGCACCCCAGGCCCTACCATGGGTCGCTCTCAGGG TTCTCCCATGGACCCCATGGTGATGAAGCGGCCGCAGCTCTATGGTATGGGCACTCACCCTCATTCCCAA CCCCAGCAGTCTTCACCATACCCTGGCGGTTCTTACGGCCCCCCCGGGGCTCAGCGCTATCCCCTTGGAA TGCAGGGTCGAGCGCCTGGGGCTCTCGGCGGCCTGCAATACCCTCAGCAGCAGATGCCACCGCAGTACGG CCAACAGGCTGTGAGTGGATACTGTCAGCAGGGGCAGCAGCCGTACTATAATCAGCAGCCGCAGCCAAGT CACCTCCCTCCCCAGGCCCAGTATCTTCAACCTGCAGCCGCGCAGTCACAGCAGCGATACCAACCTCAGC AGGACATGTCCCAGGAGGGATATGGAACTCGGAGCCAACCACCCCTCGCACCAGGCAAGTCAAACCATGA AGATTTGAACCTGATCCAGCAGGAGCGACCTAGTAGTCTGCCTGACCTGTCTGGCAGCATCGACGACCTC CCCACCGGCACTGAGGCAACCCTGTCTTCAGCTGTGTCAGCTTCCGGGAGCACCTCTTCTCAGGGCGACC AGTCCAACCCGGCTCAATCTCCATTTTCCCCTCATGCGTCCCCACACTTGTCCTCAATACCCGGGGGTCC CTCTCCAAGCCCAGTCGGTTCTCCGGTGGGGTCAAACCAGAGCCGGAGTGGTCCCATTTCACCTGCAAGT ATCCCCGGTAGCCAGATGCCTCCACAGCCACCCGGCAGCCAGAGCGAGTCCAGCAGCCACCCCGCGCTTT CTCAGTCCCCTATGCCGCAAGAGCGCGGCTTCATGACAGGAACCCAACGCAATCCTCAGATGAGCCAGTA TGGGCCCCAACAGACCGGTCCATCTATGTCCCCACATCCATCTCCTGGAGGCCAGATGCATCCAGGAATC AGCAACTTCCAACAGTCTAACTCATCAGGGACCTACGGCCCCCAGATGAGCCAGTACGGCCCCCAGGGCA ATTATTCTCGGACACCCACCTACAGCGGTGTCCCGAGTGCGAGCTATAGCGGGCCCGGTCCTGGCATGGG GATCAACGCAAATAATCAAATGCACGGCCAGGGTCCCGCCCAGCCCTGCGGGGCCATGCCCCTGGGGCGC ATGCCCTCCGCTGGAATGCAGAATAGGCCATTCCCAGGGACCATGTCAAGCGTGACACCCAGTTCCCCTG GCATGTCCCAGCAAGGCGGACCAGGCATGGGCCCACCTATGCCTACTGTAAACCGCAAAGCTCAGGAGGC TGCCGCGGCGGTTATGCAGGCCGCTGCTAACTCCGCCCAATCTAGACAGGGTAGTTTCCCGGGCATGAAT CAGTCTGGCCTGGTTGCCAGTAGTTCCCCCTACTCCCAGTCCATGAACAACAACAGCTCTCTGATGAGTA CACAGGCACAACCATACTCCATGACCCCAACTATGGTGAACAGTTCCACTGCCTCTATGGGCTTGGCCGA CATGATGTCCCCTTCCGAATCCAAACTGTCCGTGCCTCTGAAGGCCGATGGCAAGGAAGAAGGCGTCAGT CAGCCAGAGAGCAAATCTAAAGATTCTTACGGGAGTCAGGGCATCAGCCAGCCCCCTACACCTGGCAACT TGCCCGTTCCTAGCCCCATGTCACCTTCAAGTGCAAGCATTTCAAGTTTTCACGGGGATGAGTCCGACTC TATAAGCTCTCCCGGTTGGCCAAAAACCCCATCCAGCCCCAAAAGTAGTAGCTCCAGCACCACCGGTGAA AAGATAACAAAAGTCTACGAACTGGGGAACGAGCCAGAGCGAAAGCTCTGGGTCGATCGATACTTGACGT TCATGGAGGAGAGGGGCAGTCCAGTGTCCAGTCTCCCAGCCGTTGGAAAGAAGCCCCTTGACCTGTTTCG CCTCTACGTCTGTGTTAAAGAAATCGGCGGCCTGGCACAGGTGAACAAAAATAAGAAGTGGAGGGAGTTG GCAACAAATCTGAATGTTGGGACATCATCATCCGCGGCCAGCTCATTGAAGAAGCAATACATTCAATACC TCTTCGCGTTTGAATGTAAAACCGAAAGGGGCGAGGAGCCACCACCTGAGGTGTTTTCTACCGGCGATTC TAAGAAGCAGCCGAAGCTCCAACCCCCGTCCCCCGCCAATAGCGGCAGTCTGCAGGGGCCCCAGACCCCT CAGTCCACCGGCTCTAATAGTATGGCGGAGGTCCCCGGAGATCTTAAGCCACCAACCCCAGCCTCTACGC CACATGGCCAAATGACCCCCATGCAGAGTGGCAGGTCATCAACGGTGTCCGTTCACGACCCCTTTTCTGA TGTGTCCGATAGTGCATACCCCAAGCGGAACTCAATGACCCCAAACGCTCCTTACCAGCAGGGAATGGGA ATGCCTGACATGATGGGGAGGATGCCATATGAGCCGAACAAGGACCCTTTCTCAGGTATGAGGAAGGTGC CTGGCAGTTCCGAGCCATTTATGACACAGGGACAGGTGCCAAACTCCGGAATGCAGGACATGTATAACCA GAGCCCATCCGGAGCAATGTCCAATCTGGGAATGGGCCAAAGGCAGCAATTCCCCTACGGTACCTCATAC GACCGACGGCATGAAGCTTACGGACAGCAGTACCCTGGCCAAGGGCCACCCACAGGACAACCACCCTATG GCGGTCACCAACCAGGCCTCTACCCCCAGCAGCCGAACTACAAACGGCACATGGACGGAATGTACGGGCC GCCAGCCAAGAGGCACGAGGGAGATATGTACAACATGCAGTATGGAAGCCAGCAACAAGAGATGTACAAT CAATATGGTGGCTCCTATTCCGGACCCGACAGGCGACCAATCCAGGGACAGTATCCGTACCCATATAACA GAGAGCGCATGCAGGGGCCTGGGCAGATGCAGCCCCACGGAATCCCCCCTCAGATGATGGGTGGGCCAAT GCAAAGCTCCTCAAGTGAGGGGCCCCAGCAGAACATGTGGGCCACACGGAATGATATGCCGTATCCCTAT CAGTCAAGACAGGGCCCAGGCGGGCCAGCACAAGCACCACCATATCCAGGTATGAATAGGACTGACGACA TGATGGTTCCCGAGCAACGAATCAATCACGAGTCTCAGTGGCCATCTCATGTAAGTCAGCGGCAGCCTTA CATGTCTTCCTCCGCTAGCATGCAGCCAATCACTAGGCCGCCTCAGTCTAGTTATCAGACCCCTCCATCC CTGCCTAATCATATCAGCAGGGCTCCTAGCCCCGCCTCCTTTCAGCGAAGCCTGGAGTCTAGAATGTCTC CAAGCAAGTCACCGTTTCTCCCAACTATGAAGATGCAGAAAGTGATGCCTACAGTGCCTACATCTCAGGT GACAGGCCCTCCTCCACAACCACCACCGATTCGCCGGGAGATTACCTTTCCCCCAGGCAGCGTCGAAGCA TCCCAGCCTATTCTCAAGCAACGGCGGAAGATTACAAGTAAGGACATTGTCACCCCCGAGGCATGGCGCG TAATGATGTCCCTTAAATCAGGGCTGCTCGCGGAGAGCACATGGGCCCTTGACACTATTAATATCCTGCT GTATGATGATAGTACAGTGGCCACTTTCAACCTGTCTCAATTGTCCGGGTTCCTTGAGCTCCTGGTTGAG TATTTCCGAAAGTGTCTGATCGATATTTTCGGCATCCTGATGGAATACGAAGTTGGTGACCCCTCCCAGA AGGCTCTCGACCACAGGTCCGGTAAAAAGGACGACTCACAGAGCCTGGAGGATGACTCAGGTAAGGAGGA CGACGACGCAGAGTGTCTGGTGGAGGAAGAAGAAGAGGAGGAGGAGGAAGAAGAGGACTCCGAAAAAATC GAGTCCGAGGGAAAGTCCAGTCCTGCTCTGGCCGCTCCCGACGCTTCCGTGGATCCCAAAGAGACTCCCA AGCAAGCTTCTAAATTCGACAAACTGCCTATTAAGATCGTGAAGAAAAACAAACTGTTTGTTGTGGACAG ATCCGATAAACTCGGTAGGGTTCAAGAGTTTTCCTCCGGCCTGCTGCACTGGCAGCTGGGTGGCGGTGAT ACAACTGAGCACATCCAAACTCATTTTGAAAGTAAGATGGAAATCCCTCCACGACGCAGACCACCACCTC CGCTGTCTTCCACAGGAAAAAAGAAAGAGTTGGAGGGAAAGGGGGATTCCGAAGAACAGCCCGAAAAGAG CATTATTGCCACAATTGACGATGTGCTTTCTGCTAGGCCGGGAGCTCTGCCTGAGGACACAAATCCAGGC CCTCAGACCGACAGCGGGAAGTTCCCATTTGGCATCCAGCAGGCTAAGAGTCACCGGAATATTCGCCTGC TTGAAGATGAGCCACGGTCACGCGACGAGACACCGTTGTGTACGATCGCACATTGGCAAGATAGCCTTGC GAAGCGGTGTATCTGCGTGTCTAACATAGTCCGGTCCTTGTCCTTTGTCCCCGGCAACGATGCCGAGATG TCTAAACACCCTGGGCTGGTTCTCATACTCGGGAAGCTGATTCTGCTCCATCACGAACATCCTGAGAGGA AGCGCGCCCCACAGACATACGAAAAAGAAGAGGATGAGGATAAAGGAGTGGCATGCAGTAAAGACGAGTG GTGGTGGGACTGTCTCGAGGTGCTGAGAGATAACACCTTGGTCACTCTGGCTAACATATCCGGGCAGCTG GATCTTTCCGCCTATACAGAGTCTATTTGCCTCCCTATACTGGATGGCCTGCTGCACTGGATGGTGTGTC CATCAGCCGAAGCTCAAGACCCTTTCCCAACCGTCGGCCCAAACAGTGTGCTGTCCCCACAGCGCCTGGT GCTCGAGACACTCTGCAAGCTTTCTATTCAGGACAATAATGTCGATCTCATTCTCGCGACACCCCCTTTC TCCCGGCAGGAGAAATTTTACGCCACCCTGGTTCGCTATGTAGGCGATAGAAAGAATCCCGTCTGCCGGG AGATGAGCATGGCACTGCTCTCTAATCTGGCCCAGGGAGACACGCTGGCTGCCCGCGCCATTGCCGTCCA AAAAGGCAGTATAGGGAACCTGATCTCTTTTCTGGAAGATGGCGTAACCATGGCACAGTACCAACAATCA CAGCACAATCTGATGCATATGCAACCTCCACCCCTGGAGCCCCCCAGCGTGGACATGATGTGTCGGGCCG CCAAGGCCCTTTTGGCCATGGCACGGGTGGACGAAAACCGGAGCGAGTTTCTGCTCCATGAGGGAAGACT GCTCGACATTAGTATAAGTGCTGTTCTCAATTCTCTCGTGGCCTCTGTGATCTGCGACGTGCTTTTCCAG ATCGGACAGCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG214151 representing NM_001085355
Red=Cloning site Green=Tags(s) METGLLPNHKLKAVGEAPAAPPHQQHHHHHAHHHHHHHAHHLHHLHHHHALQQQLNQFQQPQPPQPQQQQ PPPPPQQQHPTANNSLGGAGGGAPQPGPDMEQPQHGGAKDSVAGNQADPQGQPLLSKPGDEDDAPPKMGE PAGSRYEHPGLGAQQQPAPVAVPGGGGGPAAVSEFNNYYGSAAPASGGPGGRAGPCFDQHGGQQSPGMGM MHSASAAAGAPSSMDPLQNSHEGYPNSQYNHYPGYSRPGAGGGGGGGGGGGGSGGGGGGGGAGGAGGAAA AAAGAGAVAAAAAAAAAAAAAAGGGGGGGYGSSSSGYGVLSSPRQQGGGMMMGPGGGGAASLSKAAAGAA AAAGGFQRFAGQNQHPSGATPTLNQLLTSPSPMMRSYGGSYPDYSSSSAPPPPSQPQSQAAAGAAAGGQQ AAAGMGLGKDLGAQYAAASPAWAAAQQRSHPAMSPGTPGPTMGRSQGSPMDPMVMKRPQLYGMGTHPHSQ PQQSSPYPGGSYGPPGAQRYPLGMQGRAPGALGGLQYPQQQMPPQYGQQAVSGYCQQGQQPYYNQQPQPS HLPPQAQYLQPAAAQSQQRYQPQQDMSQEGYGTRSQPPLAPGKSNHEDLNLIQQERPSSLPDLSGSIDDL PTGTEATLSSAVSASGSTSSQGDQSNPAQSPFSPHASPHLSSIPGGPSPSPVGSPVGSNQSRSGPISPAS IPGSQMPPQPPGSQSESSSHPALSQSPMPQERGFMTGTQRNPQMSQYGPQQTGPSMSPHPSPGGQMHPGI SNFQQSNSSGTYGPQMSQYGPQGNYSRTPTYSGVPSASYSGPGPGMGINANNQMHGQGPAQPCGAMPLGR MPSAGMQNRPFPGTMSSVTPSSPGMSQQGGPGMGPPMPTVNRKAQEAAAAVMQAAANSAQSRQGSFPGMN QSGLVASSSPYSQSMNNNSSLMSTQAQPYSMTPTMVNSSTASMGLADMMSPSESKLSVPLKADGKEEGVS QPESKSKDSYGSQGISQPPTPGNLPVPSPMSPSSASISSFHGDESDSISSPGWPKTPSSPKSSSSSTTGE KITKVYELGNEPERKLWVDRYLTFMEERGSPVSSLPAVGKKPLDLFRLYVCVKEIGGLAQVNKNKKWREL ATNLNVGTSSSAASSLKKQYIQYLFAFECKTERGEEPPPEVFSTGDSKKQPKLQPPSPANSGSLQGPQTP QSTGSNSMAEVPGDLKPPTPASTPHGQMTPMQSGRSSTVSVHDPFSDVSDSAYPKRNSMTPNAPYQQGMG MPDMMGRMPYEPNKDPFSGMRKVPGSSEPFMTQGQVPNSGMQDMYNQSPSGAMSNLGMGQRQQFPYGTSY DRRHEAYGQQYPGQGPPTGQPPYGGHQPGLYPQQPNYKRHMDGMYGPPAKRHEGDMYNMQYGSQQQEMYN QYGGSYSGPDRRPIQGQYPYPYNRERMQGPGQMQPHGIPPQMMGGPMQSSSSEGPQQNMWATRNDMPYPY QSRQGPGGPAQAPPYPGMNRTDDMMVPEQRINHESQWPSHVSQRQPYMSSSASMQPITRPPQSSYQTPPS LPNHISRAPSPASFQRSLESRMSPSKSPFLPTMKMQKVMPTVPTSQVTGPPPQPPPIRREITFPPGSVEA SQPILKQRRKITSKDIVTPEAWRVMMSLKSGLLAESTWALDTINILLYDDSTVATFNLSQLSGFLELLVE YFRKCLIDIFGILMEYEVGDPSQKALDHRSGKKDDSQSLEDDSGKEDDDAECLVEEEEEEEEEEEDSEKI ESEGKSSPALAAPDASVDPKETPKQASKFDKLPIKIVKKNKLFVVDRSDKLGRVQEFSSGLLHWQLGGGD TTEHIQTHFESKMEIPPRRRPPPPLSSTGKKKELEGKGDSEEQPEKSIIATIDDVLSARPGALPEDTNPG PQTDSGKFPFGIQQAKSHRNIRLLEDEPRSRDETPLCTIAHWQDSLAKRCICVSNIVRSLSFVPGNDAEM SKHPGLVLILGKLILLHHEHPERKRAPQTYEKEEDEDKGVACSKDEWWWDCLEVLRDNTLVTLANISGQL DLSAYTESICLPILDGLLHWMVCPSAEAQDPFPTVGPNSVLSPQRLVLETLCKLSIQDNNVDLILATPPF SRQEKFYATLVRYVGDRKNPVCREMSMALLSNLAQGDTLAARAIAVQKGSIGNLISFLEDGVTMAQYQQS QHNLMHMQPPPLEPPSVDMMCRAAKALLAMARVDENRSEFLLHEGRLLDISISAVLNSLVASVICDVLFQ IGQL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001085355 |
| ORF Size | 6732 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001085355.1, NP_001078824.1 |
| RefSeq Size | 11325 bp |
| RefSeq ORF | 6735 bp |
| Locus ID | 239985 |
| UniProt ID | E9Q4N7 |
| Gene Summary | Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (PubMed:17640523). Binds DNA non-specifically.[UniProtKB/Swiss-Prot Function] |
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