Pi4ka (NM_001001983) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Pik4ca
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225146 representing NM_001001983 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGCCCAGTAGATTTTCATGGAATCTTCCAACTAGATGAAAGACGGAGAGATGCAGTGATTGCATTGG GCATTTTTCTCATTGAATCTGATCTTCAGCACAAAGATTGTGTTGTTCCTTATCTTCTTCGACTTCTCAG GGGTCTTCCAAAAGTGTATTGGGTAGAAGAAAGCACAGCTCGGAAAGGCAGAGGTAACCTCCCAGTTGCA GAGAGCTTCAGCTTCTGCCTGGTGACCTTGCTGTCTGATGTGGCTTGCAGGGACCCCTCACTTAGGGATG AGATTTTAGAGGCAATTTTGCAGGTTTTACATGTCCTTTTGGGAATGTGCCAGGCCTTGGAGATTCAAGA AAAAGAATACCTTTGCAAGTATGCGATCCCATGCCTGATAGGAATCTCAAGATCTTTTGGACGTTACAGC AATTCAGAAGAGTCTCTCCTCTCAAAGCTTTTCCCCAAAGTTTCTCCTCATTCCCTGAGAATCCCAGAAG AGCTAGAAGGTGTCCGAAGGCGTTCCTTTAATGACTTTCGCTCCATCCTGCCCAGCAACCTGCTAACTGT CTGTCAGGAGGGCACACTGAAGAGGAAAACCAGCAGTGTGTCCAGCATCTCTCAGGTTAGCCCAGAACGT GGCATACCCCCTCCCAGCTCCCCTGGAGGATCTGCATTTCACTATTTTGAAGCTTCTTGCCTACCTGATG GGACTGCCTTGGAGCCTGAATACTACTTCTCTACCATCAGCTCCAGTTTTTCTATCTCTCCCCTGTTCAA TGGTATTACCTATAAGGAGTTCTGTATTCCACTGGAAATGCTTCGGGAACTCTTAAACTTGGTGAAGAAG ATTGTTGAGGAGCCTGTTCTCAAATCCTTAGATGCAATTGTAGCCGGCGTGATGGAGGCCAATCCCAGTG CTGATCTGTACTATACAACCTTCAGTGATCCCCTGTACCTGACCATGTTCAAGATGCTTCGAGATACTCT GTACTACATGAAAGATCTGCCCACTTCCTTTGTGAAAGAGATCCATGATTTTGTGTTGGAGCAGTTCAAC ATGAGCCAAGGGGAGCTCCAAAAGATTCTGCATGACGCAGATAGGATCCACAGTGAGATGAGCCCACTCA AACTGCGCTGCCAAGCAAACGCTGCCTGTGTGGACCTCATGGTGTGGGCTGTGAAGGATGAGCAGGGTGC AGAAAACCTTTGCATCAAGCTGTCTGAAAAGCTGCAGTCCAAGACATCCAGTAAGGTCATCATTGCCCAT TTGCCCTTGCTGATCTGCTGCCTACAGGGTCTGGGCCGCCTGTGTGAAAGGTTCCCAGTGGTGGTCCACT CAGTGACGCCATCTTTGAGAGACTTTCTGGTAATTCCATCCCCAGTGCTGGTGAAGCTCTATAAGTACCA CAGTCAGTACCACACAGTTGCTGGCAGTGATATAAAAATCAGTGTCACCAATGAGCATTCCGAGTCAACT CTGAATGTCTTGCCGGGCAAGAAGAACCAGCCATCCATGTATGAGCAGCTCCGAGACATCGCCATTGACA ACATCTGCAGGTGCCTGAAGGCCGGCCTCACCGTGGACCCTGTGATCGTGGAGGCCTTCCTGGCCAGCCT CTCCAACCGGCTCTACATTTCCCAGGAGAGTGACAAGGATGCTCACTTGATTCCTGACCATACCATCCGT GCCTTGGGACACATTGCAGTAGCTTTGAGGGACACTCCAAAGGTCATGGAGCCAATTCTGCAGATCCTGC AGCAGAAATTTTGCCAGCCCCCCTCACCCCTTGATGTGCTCATCATCGACCAGCTGGGCTGCCTGGTGAT CACTGGAAATCAATACATTTATCAGGAAGTGTGGAATCTCTTCCAGCAAATCAGTGTAAAGGCCAGTTCA GTTGTGTATTCAGCCACCAAAGACTATAAAGACCATGGCTACAGGCACTGCTCCCTAGCAGTGATCAATG CCCTGGCCAACATTGCAGCCAACATTCAGGAGGAGCATCTTGTGGACGAGCTGTTGATGAACCTGCTGGA ACTGTTTGTGCAGCTGGGGCTGGAGGGGAAAAGAGCCAGTGAGAGGGCCAGTGAAAAGGGGCCTGCCCTG AAGGCTTCCAGCAGTGCAGGAAATTTGGGCGTGCTCATTCCTGTAATAGCTGTGCTCACCCGACGATTGC CACCCATCAAAGAAGCTAAACCTAGGCTACAGAAGCTTTTCCGAGATTTCTGGCTCTACTCTGTGCTAAT GGGATTCGCTGTGGAAGGCTCAGGACTCTGGCCGGAAGAGTGGTATGAGGGAGTCTGTGAAATAGCTACC AAGTCACCTCTGCTTACCTTTCCCAGCAAAGAGCCTCTGCGATCAGTCCTCCAGTACAATTCAGCTATGA AAAACGATACGGTCACCCCTGCTGAACTGAGTGAGCTCCGAAGTACCATCATCAACTTGCTAGATCCTCC TCCTGAGGTGTCTGCCCTCATCAACAAGCTGGACTTTGCCATGTCTACCTATCTCTTGTCTGTGTATCGG CTGGAGTACATGAGGGTACTGCGTTCTACAGATCCAGATCGCTTCCAGGTAATGTTCTGCTACTTTGAGG ATAAAGCTATTCAAAAAGACAAATCTGGGATGATGCAGTGTGTCATTGCTGTTGCTGACAAAGTGTTTGA TGCTTTCCTGAACATGATGGCAGAAAAAGCTAAGACCAAGGAGAATGAAGAGGAGCTGGAGCGGCATGCA CAGTTTCTGCTGGTGAACTTCAACCACATTCATAAGAGGATAAGGAGAGTAGCTGACAAATACCTATCTG GTCTGGTGGATAAGTTTCCACACTTGCTCTGGAGCGGGACTGTGCTGAAGACCATGTTGGACATCCTGCA GACCCTGTCACTATCACTAAGTGCTGATATTCACAAGGACCAACCTTACTATGACATTCCTGATGCCCCC TATCGGATCACGGTTCCTGACACATACGAAGCCCGGGAGAGCATCGTGAAAGACTTTGCTGCGCGCTGTG GAATGATCCTACAGGAAGCCATGAAGTGGGCACCCACGGTCACTAAGTCCCACCTACAGGAATATCTGAA CAAGCATCAGAACTGGGTGTCAGGACTGTCTCAGCACACAGGATTGGCCATGGCCACTGAGAGCATCCTT CATTTTGCCGGCTACAACAAGCAGAACACAACTCTTGGGGCAACTCAGCTAACTGAGCGTCCGGCCTGTG TGAAGAAAGACTACTCCAACTTCATGGCATCCTTGAATTTGCGCAACCGCTATGCCGGCGAGGTACATGG AATGATTCGGTTCTCAGGTGCCACAGGCCAGATGTCTGACCTGAACAAAATGATGGTCCAAGATCTGATC ACAGCTTTGGACCACAGTCATCCTCAGCACTATACACAAGCCATGTTCAAATTGACGGCAATGCTCATTA GCAGCAAAGATTGTGATCCACAGCTCCTTCATCATCTGTGTTGGGGTCCCCTCCGAATGTTCAATGAGCA TGGCATGGAGACTGCACTAGCCTGCTGGGAGTGGCTGCTGGCTGGCAAGAATGGAGTGGAAGTGCCGTTT ATGCGGGAGATGGCAGGAGCCTGGCACATGACAGTAGAGCAGAAGTTCGGCCTCTTTTCTGTGGAAACAA AGGAAGCAGACCCCCTGGCAGCATCAGAAGCAAGTCAGCCAAGACCTTGTCCCCCCGAAGTGACACCTCA CTACATCTGGATCGATTTCCTGGTCCAGCGGTTTGAGATTGCCAAGTACTGTAGCTCTGACCAGGTGGAG ATCTTCTCAAGCCTGCTGCAGCGCTCCATGTCCCTCAATATTGGTGGAGCCAGGGGGAGCATGAACAGGC ATGTGGCAGCCATTGGACCACGTTTCAAACTGCTGACCTTGGGGCTGTCCCTCCTGCATGCTGATGTGGT TCCAAATGCAACCATTCGAAACGTGCTTCGAGAGAAGATATACTCAACAGCCTTTGACTATTTCAGTTGT CCACCAAAGTTTCCTACTCAAGGAGAAAAGAGGCTTCGTGAAGATATAAGCATAATGATTAAGTTTTGGA CTGCCATGTTCTCAGATAAGAAGTACCTGACTGCCAGCCAGCTCGTTCCCCCAGATAATCAGGACACCCG AAGCAACTTGGATATAACTGTGGGCTCTCGACAACAGGCTACCCAGGGCTGGATCAACACTTACCCCCTC TCCAGTGGCATGTCAACCATCTCCAAAAAGTCAGGTATGTCTAAGAAAACCAACCGGGGCTCCCAGCTGC ACAAGTACTACATGAAACGCAGGACACTGCTGCTGTCACTATTGGCCACTGAGATCGAACGCCTCATCAC TTGGTACAACCCACTGTCAGCCCCAGAGCTAGAGCTGGACCAGGCAGGAGAGAACAGCGTTGCCAACTGG AGGTCCAAGTACATCAGCTTGAGTGAGAAGCAGTGGAAGGACAACGTGAACCTTGCCTGGAGCATCTCTC CATACCTCGCTGTGCAGCTGCCAGCCAGGTTCAAGAATACAGAAGCCATTGGAAATGAAGTGACCCGTCT CGTTCGCTTGGATCCTGGAGCTGTTAGTGATGTCCCTGAAGCCATCAAGTTCCTTGTCACCTGGCACACC ATCGATGCTGATGCTCCAGAGCTCAGCCATGTTTTATGCTGGGCACCCACGGACCCACCTACAGGCCTCT CCTACTTCTCCAGCATGTACCCACCACACCCTCTCACTGCACAGTATGGGGTAAAAGTCCTGCGATCCTT CCCTCCGGATGCCATTCTGTTCTACATCCCCCAGATTGTGCAGGCCCTCAGATATGACAAGATGGGCTAT GTGCGTGAGTACATTCTCTGGGCTGCAGCCAAATCCCAGCTTCTGGCACACCAGTTTATTTGGAACATGA AAACTAACATCTACCTTGATGAAGAAGGGCACCAGAAAGACCCTGACATCGGTGACCTCCTGGAGCAGTT AGTAGAAGAGATCACAGGGTCTCTATCAGGCCCAGCCAAAGATTTCTATCAACGGGAATTTGATTTCTTT AACAAGATCACAAACGTGTCAGCCATCATCAAGCCCTACCCTAAAGGCGATGAAAGGAAGAAGGCTTGTC TGTCAGCTCTGTCTGAAGTGAAGGTGCAGCCTGGCTGCTACCTGCCCAGCAACCCTGAAGCAATCGTGCT AGACATTGACTACAAGTCAGGGACACCAATGCAGAGTGCCGCAAAAGCGCCGTATCTGGCCAAGTTTAAG GTGAAGAGATGCGGAGTGAGTGAACTTGAAAAAGAAGGTCTGCAGTGCCGCTCAGACGCAGAGGATGAAT GCTTCAGCCAAGAGGCTGATGGCAAGAAGATCTGCTGGCAAGCAGCCATCTTCAAAGTGGGGGATGACTG CCGACAGGACATGTTAGCCCTGCAGATCATCGACCTCTTCAAGAACATCTTCCAGCTGGTTGGCCTGGAC CTCTTCGTTTTTCCTTATCGGGTAGTGGCTACTGCCCCTGGGTGCGGAGTGATTGAGTGCATCCCAGACT GTACCTCCCGGGACCAGCTGGGCCGCCAGACTGACTTTGGCATGTATGACTACTTTACACGCCAGTATGG GGATGAGTCTACCTTGGCCTTCCAGCAGGCACGCTACAACTTTATCAGAAGCATGGCCGCCTATAGCCTC CTGCTGTTCCTGCTGCAGATCAAGGACAGGCACAATGGCAACATCATGCTGGACAAGAAGGGGCACATCA TCCACATCGACTTTGGCTTCATGTTTGAAAGCTCACCGGGTGGCAACCTTGGCTGGGAACCAGACATCAA GCTGACAGACGAGATGGTGATGATTATGGGGGGCAAAATGGAAGCCACACCCTTCAAGTGGTTCATGGAG ATGTGTGTCCGAGGTTATCTGGCTGTGCGCCCCTATATGGATGCAGTCGTCTCCCTGGTTACACTCATGT TGGACACAGGCCTGCCCTGTTTTCGTGGCCAGACAATCAAACTTTTGAAACACAGGTTCAGCCCGAACAT GACAGAGCGGGAGGCTGCGAATTTTATCATGAAGGTCATCCAGAACTGTTTCCTCAGCAACAGGAGCCGG ACCTACGACATGATCCAGTACTACCAGAATGATATCCCCTACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001001983
Insert Size	6135 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001001983.2, NP_001001983.2
RefSeq Size	6449 bp
RefSeq ORF	6135 bp
Locus ID	224020
UniProt ID	E9Q3L2
Gene Summary	Acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate.[UniProtKB/Swiss-Prot Function]

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