Pi4ka (NM_001001983) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Pik4ca
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR212108 representing NM_001001983 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGCCCAGTAGATTTTCATGGAATCTTCCAACTAGATGAAAGACGGAGAGATGCAGTGATTGCATTGG GCATTTTTCTCATTGAATCTGATCTTCAGCACAAAGATTGTGTTGTTCCTTATCTTCTTCGACTTCTCAG GGGTCTTCCAAAAGTGTATTGGGTAGAAGAAAGCACAGCTCGGAAAGGCAGAGGTAACCTCCCAGTTGCA GAGAGCTTCAGCTTCTGCCTGGTGACCTTGCTGTCTGATGTGGCTTGCAGGGACCCCTCACTTAGGGATG AGATTTTAGAGGCAATTTTGCAGGTTTTACATGTCCTTTTGGGAATGTGCCAGGCCTTGGAGATTCAAGA AAAAGAATACCTTTGCAAGTATGCGATCCCATGCCTGATAGGAATCTCAAGATCTTTTGGACGTTACAGC AATTCAGAAGAGTCTCTCCTCTCAAAGCTTTTCCCCAAAGTTTCTCCTCATTCCCTGAGAATCCCAGAAG AGCTAGAAGGTGTCCGAAGGCGTTCCTTTAATGACTTTCGCTCCATCCTGCCCAGCAACCTGCTAACTGT CTGTCAGGAGGGCACACTGAAGAGGAAAACCAGCAGTGTGTCCAGCATCTCTCAGGTTAGCCCAGAACGT GGCATACCCCCTCCCAGCTCCCCTGGAGGATCTGCATTTCACTATTTTGAAGCTTCTTGCCTACCTGATG GGACTGCCTTGGAGCCTGAATACTACTTCTCTACCATCAGCTCCAGTTTTTCTATCTCTCCCCTGTTCAA TGGTATTACCTATAAGGAGTTCTGTATTCCACTGGAAATGCTTCGGGAACTCTTAAACTTGGTGAAGAAG ATTGTTGAGGAGCCTGTTCTCAAATCCTTAGATGCAATTGTAGCCGGCGTGATGGAGGCCAATCCCAGTG CTGATCTGTACTATACAACCTTCAGTGATCCCCTGTACCTGACCATGTTCAAGATGCTTCGAGATACTCT GTACTACATGAAAGATCTGCCCACTTCCTTTGTGAAAGAGATCCATGATTTTGTGTTGGAGCAGTTCAAC ATGAGCCAAGGGGAGCTCCAAAAGATTCTGCATGACGCAGATAGGATCCACAGTGAGATGAGCCCACTCA AACTGCGCTGCCAAGCAAACGCTGCCTGTGTGGACCTCATGGTGTGGGCTGTGAAGGATGAGCAGGGTGC AGAAAACCTTTGCATCAAGCTGTCTGAAAAGCTGCAGTCCAAGACATCCAGTAAGGTCATCATTGCCCAT TTGCCCTTGCTGATCTGCTGCCTACAGGGTCTGGGCCGCCTGTGTGAAAGGTTCCCAGTGGTGGTCCACT CAGTGACGCCATCTTTGAGAGACTTTCTGGTAATTCCATCCCCAGTGCTGGTGAAGCTCTATAAGTACCA CAGTCAGTACCACACAGTTGCTGGCAGTGATATAAAAATCAGTGTCACCAATGAGCATTCCGAGTCAACT CTGAATGTCTTGCCGGGCAAGAAGAACCAGCCATCCATGTATGAGCAGCTCCGAGACATCGCCATTGACA ACATCTGCAGGTGCCTGAAGGCCGGCCTCACCGTGGACCCTGTGATCGTGGAGGCCTTCCTGGCCAGCCT CTCCAACCGGCTCTACATTTCCCAGGAGAGTGACAAGGATGCTCACTTGATTCCTGACCATACCATCCGT GCCTTGGGACACATTGCAGTAGCTTTGAGGGACACTCCAAAGGTCATGGAGCCAATTCTGCAGATCCTGC AGCAGAAATTTTGCCAGCCCCCCTCACCCCTTGATGTGCTCATCATCGACCAGCTGGGCTGCCTGGTGAT CACTGGAAATCAATACATTTATCAGGAAGTGTGGAATCTCTTCCAGCAAATCAGTGTAAAGGCCAGTTCA GTTGTGTATTCAGCCACCAAAGACTATAAAGACCATGGCTACAGGCACTGCTCCCTAGCAGTGATCAATG CCCTGGCCAACATTGCAGCCAACATTCAGGAGGAGCATCTTGTGGACGAGCTGTTGATGAACCTGCTGGA ACTGTTTGTGCAGCTGGGGCTGGAGGGGAAAAGAGCCAGTGAGAGGGCCAGTGAAAAGGGGCCTGCCCTG AAGGCTTCCAGCAGTGCAGGAAATTTGGGCGTGCTCATTCCTGTAATAGCTGTGCTCACCCGACGATTGC CACCCATCAAAGAAGCTAAACCTAGGCTACAGAAGCTTTTCCGAGATTTCTGGCTCTACTCTGTGCTAAT GGGATTCGCTGTGGAAGGCTCAGGACTCTGGCCGGAAGAGTGGTATGAGGGAGTCTGTGAAATAGCTACC AAGTCACCTCTGCTTACCTTTCCCAGCAAAGAGCCTCTGCGATCAGTCCTCCAGTACAATTCAGCTATGA AAAACGATACGGTCACCCCTGCTGAACTGAGTGAGCTCCGAAGTACCATCATCAACTTGCTAGATCCTCC TCCTGAGGTGTCTGCCCTCATCAACAAGCTGGACTTTGCCATGTCTACCTATCTCTTGTCTGTGTATCGG CTGGAGTACATGAGGGTACTGCGTTCTACAGATCCAGATCGCTTCCAGGTAATGTTCTGCTACTTTGAGG ATAAAGCTATTCAAAAAGACAAATCTGGGATGATGCAGTGTGTCATTGCTGTTGCTGACAAAGTGTTTGA TGCTTTCCTGAACATGATGGCAGAAAAAGCTAAGACCAAGGAGAATGAAGAGGAGCTGGAGCGGCATGCA CAGTTTCTGCTGGTGAACTTCAACCACATTCATAAGAGGATAAGGAGAGTAGCTGACAAATACCTATCTG GTCTGGTGGATAAGTTTCCACACTTGCTCTGGAGCGGGACTGTGCTGAAGACCATGTTGGACATCCTGCA GACCCTGTCACTATCACTAAGTGCTGATATTCACAAGGACCAACCTTACTATGACATTCCTGATGCCCCC TATCGGATCACGGTTCCTGACACATACGAAGCCCGGGAGAGCATCGTGAAAGACTTTGCTGCGCGCTGTG GAATGATCCTACAGGAAGCCATGAAGTGGGCACCCACGGTCACTAAGTCCCACCTACAGGAATATCTGAA CAAGCATCAGAACTGGGTGTCAGGACTGTCTCAGCACACAGGATTGGCCATGGCCACTGAGAGCATCCTT CATTTTGCCGGCTACAACAAGCAGAACACAACTCTTGGGGCAACTCAGCTAACTGAGCGTCCGGCCTGTG TGAAGAAAGACTACTCCAACTTCATGGCATCCTTGAATTTGCGCAACCGCTATGCCGGCGAGGTACATGG AATGATTCGGTTCTCAGGTGCCACAGGCCAGATGTCTGACCTGAACAAAATGATGGTCCAAGATCTGATC ACAGCTTTGGACCACAGTCATCCTCAGCACTATACACAAGCCATGTTCAAATTGACGGCAATGCTCATTA GCAGCAAAGATTGTGATCCACAGCTCCTTCATCATCTGTGTTGGGGTCCCCTCCGAATGTTCAATGAGCA TGGCATGGAGACTGCACTAGCCTGCTGGGAGTGGCTGCTGGCTGGCAAGAATGGAGTGGAAGTGCCGTTT ATGCGGGAGATGGCAGGAGCCTGGCACATGACAGTAGAGCAGAAGTTCGGCCTCTTTTCTGTGGAAACAA AGGAAGCAGACCCCCTGGCAGCATCAGAAGCAAGTCAGCCAAGACCTTGTCCCCCCGAAGTGACACCTCA CTACATCTGGATCGATTTCCTGGTCCAGCGGTTTGAGATTGCCAAGTACTGTAGCTCTGACCAGGTGGAG ATCTTCTCAAGCCTGCTGCAGCGCTCCATGTCCCTCAATATTGGTGGAGCCAGGGGGAGCATGAACAGGC ATGTGGCAGCCATTGGACCACGTTTCAAACTGCTGACCTTGGGGCTGTCCCTCCTGCATGCTGATGTGGT TCCAAATGCAACCATTCGAAACGTGCTTCGAGAGAAGATATACTCAACAGCCTTTGACTATTTCAGTTGT CCACCAAAGTTTCCTACTCAAGGAGAAAAGAGGCTTCGTGAAGATATAAGCATAATGATTAAGTTTTGGA CTGCCATGTTCTCAGATAAGAAGTACCTGACTGCCAGCCAGCTCGTTCCCCCAGATAATCAGGACACCCG AAGCAACTTGGATATAACTGTGGGCTCTCGACAACAGGCTACCCAGGGCTGGATCAACACTTACCCCCTC TCCAGTGGCATGTCAACCATCTCCAAAAAGTCAGGTATGTCTAAGAAAACCAACCGGGGCTCCCAGCTGC ACAAGTACTACATGAAACGCAGGACACTGCTGCTGTCACTATTGGCCACTGAGATCGAACGCCTCATCAC TTGGTACAACCCACTGTCAGCCCCAGAGCTAGAGCTGGACCAGGCAGGAGAGAACAGCGTTGCCAACTGG AGGTCCAAGTACATCAGCTTGAGTGAGAAGCAGTGGAAGGACAACGTGAACCTTGCCTGGAGCATCTCTC CATACCTCGCTGTGCAGCTGCCAGCCAGGTTCAAGAATACAGAAGCCATTGGAAATGAAGTGACCCGTCT CGTTCGCTTGGATCCTGGAGCTGTTAGTGATGTCCCTGAAGCCATCAAGTTCCTTGTCACCTGGCACACC ATCGATGCTGATGCTCCAGAGCTCAGCCATGTTTTATGCTGGGCACCCACGGACCCACCTACAGGCCTCT CCTACTTCTCCAGCATGTACCCACCACACCCTCTCACTGCACAGTATGGGGTAAAAGTCCTGCGATCCTT CCCTCCGGATGCCATTCTGTTCTACATCCCCCAGATTGTGCAGGCCCTCAGATATGACAAGATGGGCTAT GTGCGTGAGTACATTCTCTGGGCTGCAGCCAAATCCCAGCTTCTGGCACACCAGTTTATTTGGAACATGA AAACTAACATCTACCTTGATGAAGAAGGGCACCAGAAAGACCCTGACATCGGTGACCTCCTGGAGCAGTT AGTAGAAGAGATCACAGGGTCTCTATCAGGCCCAGCCAAAGATTTCTATCAACGGGAATTTGATTTCTTT AACAAGATCACAAACGTGTCAGCCATCATCAAGCCCTACCCTAAAGGCGATGAAAGGAAGAAGGCTTGTC TGTCAGCTCTGTCTGAAGTGAAGGTGCAGCCTGGCTGCTACCTGCCCAGCAACCCTGAAGCAATCGTGCT AGACATTGACTACAAGTCAGGGACACCAATGCAGAGTGCCGCAAAAGCGCCGTATCTGGCCAAGTTTAAG GTGAAGAGATGCGGAGTGAGTGAACTTGAAAAAGAAGGTCTGCAGTGCCGCTCAGACGCAGAGGATGAAT GCTTCAGCCAAGAGGCTGATGGCAAGAAGATCTGCTGGCAAGCAGCCATCTTCAAAGTGGGGGATGACTG CCGACAGGACATGTTAGCCCTGCAGATCATCGACCTCTTCAAGAACATCTTCCAGCTGGTTGGCCTGGAC CTCTTCGTTTTTCCTTATCGGGTAGTGGCTACTGCCCCTGGGTGCGGAGTGATTGAGTGCATCCCAGACT GTACCTCCCGGGACCAGCTGGGCCGCCAGACTGACTTTGGCATGTATGACTACTTTACACGCCAGTATGG GGATGAGTCTACCTTGGCCTTCCAGCAGGCACGCTACAACTTTATCAGAAGCATGGCCGCCTATAGCCTC CTGCTGTTCCTGCTGCAGATCAAGGACAGGCACAATGGCAACATCATGCTGGACAAGAAGGGGCACATCA TCCACATCGACTTTGGCTTCATGTTTGAAAGCTCACCGGGTGGCAACCTTGGCTGGGAACCAGACATCAA GCTGACAGACGAGATGGTGATGATTATGGGGGGCAAAATGGAAGCCACACCCTTCAAGTGGTTCATGGAG ATGTGTGTCCGAGGTTATCTGGCTGTGCGCCCCTATATGGATGCAGTCGTCTCCCTGGTTACACTCATGT TGGACACAGGCCTGCCCTGTTTTCGTGGCCAGACAATCAAACTTTTGAAACACAGGTTCAGCCCGAACAT GACAGAGCGGGAGGCTGCGAATTTTATCATGAAGGTCATCCAGAACTGTTTCCTCAGCAACAGGAGCCGG ACCTACGACATGATCCAGTACTACCAGAATGATATCCCCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR212108 representing NM_001001983 Red=Cloning site Green=Tags(s) MCPVDFHGIFQLDERRRDAVIALGIFLIESDLQHKDCVVPYLLRLLRGLPKVYWVEESTARKGRGNLPVA ESFSFCLVTLLSDVACRDPSLRDEILEAILQVLHVLLGMCQALEIQEKEYLCKYAIPCLIGISRSFGRYS NSEESLLSKLFPKVSPHSLRIPEELEGVRRRSFNDFRSILPSNLLTVCQEGTLKRKTSSVSSISQVSPER GIPPPSSPGGSAFHYFEASCLPDGTALEPEYYFSTISSSFSISPLFNGITYKEFCIPLEMLRELLNLVKK IVEEPVLKSLDAIVAGVMEANPSADLYYTTFSDPLYLTMFKMLRDTLYYMKDLPTSFVKEIHDFVLEQFN MSQGELQKILHDADRIHSEMSPLKLRCQANAACVDLMVWAVKDEQGAENLCIKLSEKLQSKTSSKVIIAH LPLLICCLQGLGRLCERFPVVVHSVTPSLRDFLVIPSPVLVKLYKYHSQYHTVAGSDIKISVTNEHSEST LNVLPGKKNQPSMYEQLRDIAIDNICRCLKAGLTVDPVIVEAFLASLSNRLYISQESDKDAHLIPDHTIR ALGHIAVALRDTPKVMEPILQILQQKFCQPPSPLDVLIIDQLGCLVITGNQYIYQEVWNLFQQISVKASS VVYSATKDYKDHGYRHCSLAVINALANIAANIQEEHLVDELLMNLLELFVQLGLEGKRASERASEKGPAL KASSSAGNLGVLIPVIAVLTRRLPPIKEAKPRLQKLFRDFWLYSVLMGFAVEGSGLWPEEWYEGVCEIAT KSPLLTFPSKEPLRSVLQYNSAMKNDTVTPAELSELRSTIINLLDPPPEVSALINKLDFAMSTYLLSVYR LEYMRVLRSTDPDRFQVMFCYFEDKAIQKDKSGMMQCVIAVADKVFDAFLNMMAEKAKTKENEEELERHA QFLLVNFNHIHKRIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDIPDAP YRITVPDTYEARESIVKDFAARCGMILQEAMKWAPTVTKSHLQEYLNKHQNWVSGLSQHTGLAMATESIL HFAGYNKQNTTLGATQLTERPACVKKDYSNFMASLNLRNRYAGEVHGMIRFSGATGQMSDLNKMMVQDLI TALDHSHPQHYTQAMFKLTAMLISSKDCDPQLLHHLCWGPLRMFNEHGMETALACWEWLLAGKNGVEVPF MREMAGAWHMTVEQKFGLFSVETKEADPLAASEASQPRPCPPEVTPHYIWIDFLVQRFEIAKYCSSDQVE IFSSLLQRSMSLNIGGARGSMNRHVAAIGPRFKLLTLGLSLLHADVVPNATIRNVLREKIYSTAFDYFSC PPKFPTQGEKRLREDISIMIKFWTAMFSDKKYLTASQLVPPDNQDTRSNLDITVGSRQQATQGWINTYPL SSGMSTISKKSGMSKKTNRGSQLHKYYMKRRTLLLSLLATEIERLITWYNPLSAPELELDQAGENSVANW RSKYISLSEKQWKDNVNLAWSISPYLAVQLPARFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHT IDADAPELSHVLCWAPTDPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGY VREYILWAAAKSQLLAHQFIWNMKTNIYLDEEGHQKDPDIGDLLEQLVEEITGSLSGPAKDFYQREFDFF NKITNVSAIIKPYPKGDERKKACLSALSEVKVQPGCYLPSNPEAIVLDIDYKSGTPMQSAAKAPYLAKFK VKRCGVSELEKEGLQCRSDAEDECFSQEADGKKICWQAAIFKVGDDCRQDMLALQIIDLFKNIFQLVGLD LFVFPYRVVATAPGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSL LLFLLQIKDRHNGNIMLDKKGHIIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFME MCVRGYLAVRPYMDAVVSLVTLMLDTGLPCFRGQTIKLLKHRFSPNMTEREAANFIMKVIQNCFLSNRSR TYDMIQYYQNDIPY TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001001983
ORF Size	6132 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001001983.2, NP_001001983.2
RefSeq Size	6449 bp
RefSeq ORF	6135 bp
Locus ID	224020
UniProt ID	E9Q3L2
MW	231.4 kDa
Gene Summary	Acts on phosphatidylinositol (PtdIns) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate.[UniProtKB/Swiss-Prot Function]

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