Trpm6 (NM_153417) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	CHAK2; TRPM6/CHAK2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225134 representing NM_153417 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGTCAAGAAATCCTGGATCGAAGGGGTATTCTACAAGAGAGAGTGTAACAAATTCATACCCAGCT CAAAAGACCCTCACAGATGCACTCCAGGCTGCCAAATCTGCCACAATTTAGTCAGGTGTTACTGTGGCCG GCTGATTGAAGAACACCATGGGCTGGATCGTGCCTGGAACCTCTCAGTCACTGAGGGCCATGGGGATGAG CAGTGGTCTGTGGAGAAGCACACAGTGAAAAGCCCTACAGATACCTTCGGCACCATTAATTTCCAAGATG GAGAACATATCCACCACTCCAAGTACATTAGAACTTCTTGGGATACAAAATCGGATCATCTGTTGCACTT AATGCTGAAAGAGTGGAATATGGAACTGCCAAAGCTTGTGATCTCCGTCCATGGGGGTCTTCAGAACTTC AAGATATCCTCCAAGCTCAAAGAGACCTTCAGCCAGGGTCTGGTCAAAGCTGCAGAGACCACAGGAGCAT GGATAATTACAGAAGGCATAAACTCAGGAGTGTCCAAGCACGTTGGGGATGCCCTAAAAGCCCATTCCTC TAAGTCTTTGAGGAAAATCTGGACAGTTGGAATCCCTCCTTGGGGAGTCATTGAGAACCAGAGAGAGCTG GTCGGAAAAGATGTGGTGTGTATGTACCAGACACTGGGTAACCCACTCAGCAAGCTCACCACGCTAAACT GTATGCACTCTCACTTCATCCTGTGTGATGATGGGACTGTGGGCATGTATGGAAATGAAGAGAAGCTCAG GCGGAACCTGGAGAAGCATCTCTCAATGCAGAAGATACACACTTGCTCAAGACAAGGTGTGCCTGTGGTG GGGCTGGTGATGGAAGGAGGTCCCAATGTCATCCTCTGGGTATGGGAGACCGTCAAGAACAAGGAGCCCG TGGTGGTGTGCGAGGGGACAGGCAGAGCTGCTGACCTCTTGGCCTTCACTTACAAACATTTGGAGGATGG AGGGATTCTACGCCCTCAGGTGAAAGAAGAGCTCTTCTGTTTGATTCAGAACATGTTCAACTTTAGCCTT AGACAGTCTAAGCACCTTTTCCAAATTCTAATGGAGTGCATGGTACACAAGGATTCTATCACCATATTTG ATGCTGATTCTGAAGAGCACCAAGATCTTGATTTGGCCATCCTCACGGCTCTACTGAAGGGTACAAGCTT ATCAATATCAGAACAATTAAATCTGGCAATGGCTTGGGATAGAATGGACATTGCCAAGAAACATATCCTA ACTTATGGACAACATTGGAAGCCTGGTGCTCTAGAACAAGCAATGCTAGATGCTTTAGTGATGGATCGAG TGGACTTTGTGAAACTTTTGATAGAGAATGGGGTGAACCTCCACCGCTTCCTCACCATCCCTCGGCTGGA AGAGCTCTATAACACAAAACAAGGACCAACTAATAAATTTTTGCGTCATCTTGTCCAAGACGTAAAGCAG CACACCCTTCTCTCGAGCTATAGAATAACACTCATTGACATTGGGCTGGTAATAGAGTACCTCATTGGTG GAGCATATCGGAGCAGCTACACTAGGAAAAGTTTCAGAATTCTCTACAACAACCTCTACAGAAAACACAA GAGTGTGAGCAGCTTTGCTCAGGGCCTCTCCCAGCACTCTCTTCATCAGAGACATTCACTGAGAAATAGA AAGGAGTCTTCTGAAAGCACACTGCACTCCCAGTTCTTCAGGACGGCCCAGCCCTACAAATCCAAGGAGA AGCCTGAGGACTCTCAAAAATCAAAGAAGAAGTCCAAGGAAAGACAAAGCCTGTCAGAGGAACCCGAGGC TGCTGGCTTTATCTACCCATACAATGACCTTCTGGTGTGGGCGGTGCTCATGAAGAGGCAGAATATGGCC ATGTTCTTCTGGCAGCATGGAGAGGAGGCCACAGTCAAGGCGGTCATTGCTAGTATCCTCTACAGAGCCA TGGCCCGGGAAGCCAAGGAGAGCAACATGGTGGATGACACTTCAGAAGAGTTGAAAAATTACTCAGAGCA GTTTGGCCAGCTCGCCCTGGATGTATTAGAGAAAGCCTTCAAGCAGAACGAGCCGATGGCCATGAAGCTG CTGACCTATGAGCTCAAGAATTGGAGTAATTCCACCTGCTTGAAACTGGCGGTCTCTGGAGGTCTGCGCC CTTTTGTGTCGCATTCTTGTACCCAAATGCTGCTAACAGACATGTGGATGGGGCGTCTGAAAATGAGAAA AAATTCCTGGCTGAAGATAATCATCAGTATCCTCTTACCACCCATGATTTTGACATTGGAATTTAAAAGC AAAGCAGAGATGTCACATGTCCCACAGTCCCAGGATTTCCAGTTTACATGGAATTACAGTGACCAGGGCC TGAGTAACACCAAAGAAAGTGCTTGTGTGAAAGACTATGATTTGGAAAGAGGCCCTGATGAGAAACCGGA TGAACCTCTGCATTTAGACTTAAGAAATGTGCCCCAGAGCCTCCCCTGGACCCGGAGAGTCTACGAGTTC TACAGCGCTCCGTTTGTCAAGTTTTGGTTCTACACGATGGCTTATTTGGCATTCCTCATGCTGTTCACTT ACACAGTGTTGGTGGAGATGCAGCCCCAACCCAGCGTGCACGAATGGCTTGTCATCATCTACATCTTCAC CAATGCTATTGAGAAGGTCAGGGAGATCTGCATTTCAGAACCAAGCAAGTTTAAGCAGAAGGTGAAGATG TGGCTCAGTGAGTACTGGAACCTCATGGAAACTGTGGCTATTGGCCTGTTTGCTGTTGGCTTTGGCCTAC GGTGGGGGCATCCACCTTTGCAAACTGCAGGGAGACTCATCTACTGCATAGACATCATATTTTGGTTTTC TCGGCTCATGGACTTCTTTGCTGTGAATCAACATGCAGGTCCATATGTGACCATGATTGCCAAAATGGCA GCAAACATGTTCTACATTGTGATCATCATGGCCATTGTCCTGCTGAGCTTTGGCGTGGCGCGCAAAGCTA TCCTCTCACCGAAGGAGCCTCCTTCATGGCGCCTGGCTCGTGACATCGTCTTTGAGCCGTATTGGATGAT GTATGGAGAAGTCTATGCTTCAGATATAGATGTTTGTTCAAATGAGACTTCTTGCCCTCCGGGTTCTTTC CTGACTCCATTCCTGCAAGCTGTCTACCTCTTTGTGCAATACATCATCATGGTGAACCTTCTGATTGCCT GTTTCAACAACATTTACTTAGATATAAAATCCATCTCGAACAAGCTCTGGAAATACAACCGCTATCGCTA CATCATGACCTACCACCAGAAGCCCTGGCTGCCTCCTCCCTTTATCCTGCTGAATCACCTGTGTCTCCTC CTCCGAGGTCTGTGCTGTCGCCCAGCCCCACAGGACCAGGAAGAGGGGGACGGAGGGCTAAAGCTCTATC TCACTAAGGATGACTTGAAAAAGCTTCATGATTTTGAAGAGCAGTGTGTGGAAAAATATTTCCACGAGAA GACGGAAGGTCTGAATTGTAGTTTTGAGGAACAAATTCGAATGACATCAGAAAGGGTATCAGAGATGTTT TTCCAACTGAAAGAAATGAATGAAAAAGTATCTTTTATAAAGGACTCCCTACTATCCCTGGACAGCCAGG TGGGACACCTTCAGGACCTCTCGGCCATAACTGTTGACACCTTAAAGGTCCTTTCTGCTGTTGACACCTT GCAAGAAGATGAGATTCTTTTGGCCAACAGAAAGCATTCTACTTGCAGAAAACGTCCCCACAGCTGGACC AATGTCATCTGTGCAAAGGTTCTAAGTGACATGGAAAGCTGTGGGAAGAAGAAACTTCAGTATTATAGCA TGCCCCCTTCTTTGCTGAGGAGCCTCGCTAGAAGTCAGCTTCCCCCAAGTGTGCAGAGGGGTGCACTTGT AGAGGTTACGCACAGTAAGAGAGAGGCCTCACATGTAAGAGAAGAGCAGGAGGAACGAGAAATGGAACAG AGGACAACGGCTTCTGGGATTTCCCATGTCAGACAAGCACACTCTAAGTATGGCCAGTTTCTCCTGGTTC CATCTTCTGGAAAGCAAGTTCCTTTGTCTTTGGAAACCCCGCCACATCTTTTCAGATCATCTGAGGAGGC AGGTATAGATGGTCTGGTATTGGAGCATATACACCAAAGTGATCTCACCACCCACTTGCCTCAGCAGACC CCTGCTGCCTCCCACCAGGCATTGGTAGCGGAACACAAAGACCAACATGAGGCAGTTACACAGATGTCAG ACAAACCTGCCAAGGCAGAACAAGACTTGCTCGCATTCAGTGGCACACCTGCTCCCATGACAGTGACTTC TCTTCCTTCGAGAGCCATCAGCATGCAAGATGAAGGTGGATATGTAAACTGGGCATTTTCAGAAAATGAT GAAACAGGTGTGTTTAGCTTTAAAAAAAAGTGGAAAACTTGCTTGGCCTCCACTTGCAATAGTGACTCCA ATCCTGGTGGAGACTACTTCCTGCACACTGGAGGTAGATCCGGACTGGATAACTCAAGAAGACTGGCCCA GAGTTGTGAGTGCCCAGCAGGACCGTGGACCCAGGCAAGAAGATCCTTTTGGATCAATCCTCTCTGCAGA GATAAAGCTCTCATTAAGAGTCATAGTTTTAGATTCCACAAGGAGGAGAAACTGAGGAAGACCTGGAAGA ACAACAGTCATTCAAAATCCTTAGAGACCAGGTCCACATGGCTCAAAGCAAAACTGCTAACCAAGACCCG GAGCCTGTCAAAGAAGAAGAGGAAGACCCAAGGGCTCCAGGTGCCGGTAATAACAGTCAATGCCTGCTAT CAAAGTGACCAGTTGAATGCAGAGCCAGGAGAAACTAACACCACTGAAGAGTTCAGCAAGAAATGGCTCT CTGTATCCAACTTCAGCCAGATAGGTTTAGAGCCTTACATATACCAGAAAATGAAAATGAAGGAAATCAA GCGACATACTACACAAGCCAGTGACCACCTAAGGCAGCCACAAGAGAACCGAGATAAAACCCCCACATGG AATTCTGGGAGCACCAGCCTCAGCAGGAGTTTTCTAACAAGAAGTCCAAATGAAGTTCACAAGATCTCAA CCTCCTTAAAAAGCCCTCAAGAGCCTCACCACCATTATTCAGCCATTGAAAGGAATAATTTAATGAGACT GTCTCAGACCATACCGTTTACACCAATCCAGCTGTTCACAGGAGAGGAAGTGACCATCTACAAGCTTGAA GAAAGTTCTCCTCTGACCCTGGATAAGAGCATGTCCTCTTGGTCGCAGCATGGCAGAGCTGCCATGATTC AGGTGCTGTCACAAGAGGAAATGGATGGGGGTCTCCGCAAAGCCATGCGAGTTATCAGCACCTGGTCTGA GGATGATGTTCTCAAGCCTGGGCAGGTTTTCATTGTGAAGTCTTTTCTCCCCGAGGTTGTGCAGACGTGG TATAAAATCTTCCAGGAGAGCACTGTGCTTCATCTTTGCCTTAGGGAAATTCAACAGCAAAGAGCTGCCC AAAAACTCATCTATACCTTTAACCAAGTAAAACCACAAACCATTCCCTATACACCAAGGTTTCTCGAGGT TTCCTTGGTCTACTGCCATTCAGCCAACCAATGGTTAACCATTGAGAAGTACATGACAGGGGAGTTCCGG AAATACAATAACAACAATGGTGATGAAATAGCTCCCACCAATACCCTGGAAGAACTGATGTTGGCTTTCT CTCACTGGACCTATGAATATACCCGGGGAGAGCTGCTGGTTTTAGATTTGCAAGGTGTTGGAGAAAATTT GACAGATCCGTCTGTTATAAAACCGGAAGACAAACAATCAAGAGGGATGGTGTTTGGACCGGCCAATTTA GGGGAAGATGCAATTAGAAGCTTCATTGCAAAACATCGCTGCAACTCCTGCTGTGGGAAGCTCAGACTGC CGGATTTAAAAAGGAATGATTACTCCCTTTCAAGAACACACTGCAACTTGGGATTTGGGCAAACCATTGA ACCAACTGAGGAGCTTCCAGAAAGAGACAAAAATAGAAGTTCCCTGGAAGATCACACACGCCTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_153417
Insert Size	6087 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_153417.1, NP_700466.1
RefSeq Size	6520 bp
RefSeq ORF	6087 bp
Locus ID	225997
UniProt ID	Q8CIR4
Gene Summary	Essential ion channel and kinase. Crucial for magnesium homeostasis. Has an important role in epithelial magnesium transport and in the active magnesium absorption in the gut and kidney (By similarity).[UniProtKB/Swiss-Prot Function]

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