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Trpm6 (NM_153417) Mouse Tagged ORF Clone
CAT＃: MG225306
- TrueORF®
Trpm6 (tGFP-tagged) - Mouse transient receptor potential cation channel subfamily M member 6 (Trpm6), (10ug)
ORF Plasmid: DDK
"NM_153417" in other vectors (2)
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CNY 17,860.00
货期*
9周
规格
Product images
经常一起买 (5)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CHAK2; TRPM6/CHAK2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG225306 representing NM_153417
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGTCAAGAAATCCTGGATCGAAGGGGTATTCTACAAGAGAGAGTGTAACAAATTCATACCCAGCT CAAAAGACCCTCACAGATGCACTCCAGGCTGCCAAATCTGCCACAATTTAGTCAGGTGTTACTGTGGCCG GCTGATTGAAGAACACCATGGGCTGGATCGTGCCTGGAACCTCTCAGTCACTGAGGGCCATGGGGATGAG CAGTGGTCTGTGGAGAAGCACACAGTGAAAAGCCCTACAGATACCTTCGGCACCATTAATTTCCAAGATG GAGAACATATCCACCACTCCAAGTACATTAGAACTTCTTGGGATACAAAATCGGATCATCTGTTGCACTT AATGCTGAAAGAGTGGAATATGGAACTGCCAAAGCTTGTGATCTCCGTCCATGGGGGTCTTCAGAACTTC AAGATATCCTCCAAGCTCAAAGAGACCTTCAGCCAGGGTCTGGTCAAAGCTGCAGAGACCACAGGAGCAT GGATAATTACAGAAGGCATAAACTCAGGAGTGTCCAAGCACGTTGGGGATGCCCTAAAAGCCCATTCCTC TAAGTCTTTGAGGAAAATCTGGACAGTTGGAATCCCTCCTTGGGGAGTCATTGAGAACCAGAGAGAGCTG GTCGGAAAAGATGTGGTGTGTATGTACCAGACACTGGGTAACCCACTCAGCAAGCTCACCACGCTAAACT GTATGCACTCTCACTTCATCCTGTGTGATGATGGGACTGTGGGCATGTATGGAAATGAAGAGAAGCTCAG GCGGAACCTGGAGAAGCATCTCTCAATGCAGAAGATACACACTTGCTCAAGACAAGGTGTGCCTGTGGTG GGGCTGGTGATGGAAGGAGGTCCCAATGTCATCCTCTGGGTATGGGAGACCGTCAAGAACAAGGAGCCCG TGGTGGTGTGCGAGGGGACAGGCAGAGCTGCTGACCTCTTGGCCTTCACTTACAAACATTTGGAGGATGG AGGGATTCTACGCCCTCAGGTGAAAGAAGAGCTCTTCTGTTTGATTCAGAACATGTTCAACTTTAGCCTT AGACAGTCTAAGCACCTTTTCCAAATTCTAATGGAGTGCATGGTACACAAGGATTCTATCACCATATTTG ATGCTGATTCTGAAGAGCACCAAGATCTTGATTTGGCCATCCTCACGGCTCTACTGAAGGGTACAAGCTT ATCAATATCAGAACAATTAAATCTGGCAATGGCTTGGGATAGAATGGACATTGCCAAGAAACATATCCTA ACTTATGGACAACATTGGAAGCCTGGTGCTCTAGAACAAGCAATGCTAGATGCTTTAGTGATGGATCGAG TGGACTTTGTGAAACTTTTGATAGAGAATGGGGTGAACCTCCACCGCTTCCTCACCATCCCTCGGCTGGA AGAGCTCTATAACACAAAACAAGGACCAACTAATAAATTTTTGCGTCATCTTGTCCAAGACGTAAAGCAG CACACCCTTCTCTCGAGCTATAGAATAACACTCATTGACATTGGGCTGGTAATAGAGTACCTCATTGGTG GAGCATATCGGAGCAGCTACACTAGGAAAAGTTTCAGAATTCTCTACAACAACCTCTACAGAAAACACAA GAGTGTGAGCAGCTTTGCTCAGGGCCTCTCCCAGCACTCTCTTCATCAGAGACATTCACTGAGAAATAGA AAGGAGTCTTCTGAAAGCACACTGCACTCCCAGTTCTTCAGGACGGCCCAGCCCTACAAATCCAAGGAGA AGCCTGAGGACTCTCAAAAATCAAAGAAGAAGTCCAAGGAAAGACAAAGCCTGTCAGAGGAACCCGAGGC TGCTGGCTTTATCTACCCATACAATGACCTTCTGGTGTGGGCGGTGCTCATGAAGAGGCAGAATATGGCC ATGTTCTTCTGGCAGCATGGAGAGGAGGCCACAGTCAAGGCGGTCATTGCTAGTATCCTCTACAGAGCCA TGGCCCGGGAAGCCAAGGAGAGCAACATGGTGGATGACACTTCAGAAGAGTTGAAAAATTACTCAGAGCA GTTTGGCCAGCTCGCCCTGGATGTATTAGAGAAAGCCTTCAAGCAGAACGAGCCGATGGCCATGAAGCTG CTGACCTATGAGCTCAAGAATTGGAGTAATTCCACCTGCTTGAAACTGGCGGTCTCTGGAGGTCTGCGCC CTTTTGTGTCGCATTCTTGTACCCAAATGCTGCTAACAGACATGTGGATGGGGCGTCTGAAAATGAGAAA AAATTCCTGGCTGAAGATAATCATCAGTATCCTCTTACCACCCATGATTTTGACATTGGAATTTAAAAGC AAAGCAGAGATGTCACATGTCCCACAGTCCCAGGATTTCCAGTTTACATGGAATTACAGTGACCAGGGCC TGAGTAACACCAAAGAAAGTGCTTGTGTGAAAGACTATGATTTGGAAAGAGGCCCTGATGAGAAACCGGA TGAACCTCTGCATTTAGACTTAAGAAATGTGCCCCAGAGCCTCCCCTGGACCCGGAGAGTCTACGAGTTC TACAGCGCTCCGTTTGTCAAGTTTTGGTTCTACACGATGGCTTATTTGGCATTCCTCATGCTGTTCACTT ACACAGTGTTGGTGGAGATGCAGCCCCAACCCAGCGTGCACGAATGGCTTGTCATCATCTACATCTTCAC CAATGCTATTGAGAAGGTCAGGGAGATCTGCATTTCAGAACCAAGCAAGTTTAAGCAGAAGGTGAAGATG TGGCTCAGTGAGTACTGGAACCTCATGGAAACTGTGGCTATTGGCCTGTTTGCTGTTGGCTTTGGCCTAC GGTGGGGGCATCCACCTTTGCAAACTGCAGGGAGACTCATCTACTGCATAGACATCATATTTTGGTTTTC TCGGCTCATGGACTTCTTTGCTGTGAATCAACATGCAGGTCCATATGTGACCATGATTGCCAAAATGGCA GCAAACATGTTCTACATTGTGATCATCATGGCCATTGTCCTGCTGAGCTTTGGCGTGGCGCGCAAAGCTA TCCTCTCACCGAAGGAGCCTCCTTCATGGCGCCTGGCTCGTGACATCGTCTTTGAGCCGTATTGGATGAT GTATGGAGAAGTCTATGCTTCAGATATAGATGTTTGTTCAAATGAGACTTCTTGCCCTCCGGGTTCTTTC CTGACTCCATTCCTGCAAGCTGTCTACCTCTTTGTGCAATACATCATCATGGTGAACCTTCTGATTGCCT GTTTCAACAACATTTACTTAGATATAAAATCCATCTCGAACAAGCTCTGGAAATACAACCGCTATCGCTA CATCATGACCTACCACCAGAAGCCCTGGCTGCCTCCTCCCTTTATCCTGCTGAATCACCTGTGTCTCCTC CTCCGAGGTCTGTGCTGTCGCCCAGCCCCACAGGACCAGGAAGAGGGGGACGGAGGGCTAAAGCTCTATC TCACTAAGGATGACTTGAAAAAGCTTCATGATTTTGAAGAGCAGTGTGTGGAAAAATATTTCCACGAGAA GACGGAAGGTCTGAATTGTAGTTTTGAGGAACAAATTCGAATGACATCAGAAAGGGTATCAGAGATGTTT TTCCAACTGAAAGAAATGAATGAAAAAGTATCTTTTATAAAGGACTCCCTACTATCCCTGGACAGCCAGG TGGGACACCTTCAGGACCTCTCGGCCATAACTGTTGACACCTTAAAGGTCCTTTCTGCTGTTGACACCTT GCAAGAAGATGAGATTCTTTTGGCCAACAGAAAGCATTCTACTTGCAGAAAACGTCCCCACAGCTGGACC AATGTCATCTGTGCAAAGGTTCTAAGTGACATGGAAAGCTGTGGGAAGAAGAAACTTCAGTATTATAGCA TGCCCCCTTCTTTGCTGAGGAGCCTCGCTAGAAGTCAGCTTCCCCCAAGTGTGCAGAGGGGTGCACTTGT AGAGGTTACGCACAGTAAGAGAGAGGCCTCACATGTAAGAGAAGAGCAGGAGGAACGAGAAATGGAACAG AGGACAACGGCTTCTGGGATTTCCCATGTCAGACAAGCACACTCTAAGTATGGCCAGTTTCTCCTGGTTC CATCTTCTGGAAAGCAAGTTCCTTTGTCTTTGGAAACCCCGCCACATCTTTTCAGATCATCTGAGGAGGC AGGTATAGATGGTCTGGTATTGGAGCATATACACCAAAGTGATCTCACCACCCACTTGCCTCAGCAGACC CCTGCTGCCTCCCACCAGGCATTGGTAGCGGAACACAAAGACCAACATGAGGCAGTTACACAGATGTCAG ACAAACCTGCCAAGGCAGAACAAGACTTGCTCGCATTCAGTGGCACACCTGCTCCCATGACAGTGACTTC TCTTCCTTCGAGAGCCATCAGCATGCAAGATGAAGGTGGATATGTAAACTGGGCATTTTCAGAAAATGAT GAAACAGGTGTGTTTAGCTTTAAAAAAAAGTGGAAAACTTGCTTGGCCTCCACTTGCAATAGTGACTCCA ATCCTGGTGGAGACTACTTCCTGCACACTGGAGGTAGATCCGGACTGGATAACTCAAGAAGACTGGCCCA GAGTTGTGAGTGCCCAGCAGGACCGTGGACCCAGGCAAGAAGATCCTTTTGGATCAATCCTCTCTGCAGA GATAAAGCTCTCATTAAGAGTCATAGTTTTAGATTCCACAAGGAGGAGAAACTGAGGAAGACCTGGAAGA ACAACAGTCATTCAAAATCCTTAGAGACCAGGTCCACATGGCTCAAAGCAAAACTGCTAACCAAGACCCG GAGCCTGTCAAAGAAGAAGAGGAAGACCCAAGGGCTCCAGGTGCCGGTAATAACAGTCAATGCCTGCTAT CAAAGTGACCAGTTGAATGCAGAGCCAGGAGAAACTAACACCACTGAAGAGTTCAGCAAGAAATGGCTCT CTGTATCCAACTTCAGCCAGATAGGTTTAGAGCCTTACATATACCAGAAAATGAAAATGAAGGAAATCAA GCGACATACTACACAAGCCAGTGACCACCTAAGGCAGCCACAAGAGAACCGAGATAAAACCCCCACATGG AATTCTGGGAGCACCAGCCTCAGCAGGAGTTTTCTAACAAGAAGTCCAAATGAAGTTCACAAGATCTCAA CCTCCTTAAAAAGCCCTCAAGAGCCTCACCACCATTATTCAGCCATTGAAAGGAATAATTTAATGAGACT GTCTCAGACCATACCGTTTACACCAATCCAGCTGTTCACAGGAGAGGAAGTGACCATCTACAAGCTTGAA GAAAGTTCTCCTCTGACCCTGGATAAGAGCATGTCCTCTTGGTCGCAGCATGGCAGAGCTGCCATGATTC AGGTGCTGTCACAAGAGGAAATGGATGGGGGTCTCCGCAAAGCCATGCGAGTTATCAGCACCTGGTCTGA GGATGATGTTCTCAAGCCTGGGCAGGTTTTCATTGTGAAGTCTTTTCTCCCCGAGGTTGTGCAGACGTGG TATAAAATCTTCCAGGAGAGCACTGTGCTTCATCTTTGCCTTAGGGAAATTCAACAGCAAAGAGCTGCCC AAAAACTCATCTATACCTTTAACCAAGTAAAACCACAAACCATTCCCTATACACCAAGGTTTCTCGAGGT TTCCTTGGTCTACTGCCATTCAGCCAACCAATGGTTAACCATTGAGAAGTACATGACAGGGGAGTTCCGG AAATACAATAACAACAATGGTGATGAAATAGCTCCCACCAATACCCTGGAAGAACTGATGTTGGCTTTCT CTCACTGGACCTATGAATATACCCGGGGAGAGCTGCTGGTTTTAGATTTGCAAGGTGTTGGAGAAAATTT GACAGATCCGTCTGTTATAAAACCGGAAGACAAACAATCAAGAGGGATGGTGTTTGGACCGGCCAATTTA GGGGAAGATGCAATTAGAAGCTTCATTGCAAAACATCGCTGCAACTCCTGCTGTGGGAAGCTCAGACTGC CGGATTTAAAAAGGAATGATTACTCCCTTTCAAGAACACACTGCAACTTGGGATTTGGGCAAACCATTGA ACCAACTGAGGAGCTTCCAGAAAGAGACAAAAATAGAAGTTCCCTGGAAGATCACACACGCCTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG225306 representing NM_153417
Red=Cloning site Green=Tags(s) MQVKKSWIEGVFYKRECNKFIPSSKDPHRCTPGCQICHNLVRCYCGRLIEEHHGLDRAWNLSVTEGHGDE QWSVEKHTVKSPTDTFGTINFQDGEHIHHSKYIRTSWDTKSDHLLHLMLKEWNMELPKLVISVHGGLQNF KISSKLKETFSQGLVKAAETTGAWIITEGINSGVSKHVGDALKAHSSKSLRKIWTVGIPPWGVIENQREL VGKDVVCMYQTLGNPLSKLTTLNCMHSHFILCDDGTVGMYGNEEKLRRNLEKHLSMQKIHTCSRQGVPVV GLVMEGGPNVILWVWETVKNKEPVVVCEGTGRAADLLAFTYKHLEDGGILRPQVKEELFCLIQNMFNFSL RQSKHLFQILMECMVHKDSITIFDADSEEHQDLDLAILTALLKGTSLSISEQLNLAMAWDRMDIAKKHIL TYGQHWKPGALEQAMLDALVMDRVDFVKLLIENGVNLHRFLTIPRLEELYNTKQGPTNKFLRHLVQDVKQ HTLLSSYRITLIDIGLVIEYLIGGAYRSSYTRKSFRILYNNLYRKHKSVSSFAQGLSQHSLHQRHSLRNR KESSESTLHSQFFRTAQPYKSKEKPEDSQKSKKKSKERQSLSEEPEAAGFIYPYNDLLVWAVLMKRQNMA MFFWQHGEEATVKAVIASILYRAMAREAKESNMVDDTSEELKNYSEQFGQLALDVLEKAFKQNEPMAMKL LTYELKNWSNSTCLKLAVSGGLRPFVSHSCTQMLLTDMWMGRLKMRKNSWLKIIISILLPPMILTLEFKS KAEMSHVPQSQDFQFTWNYSDQGLSNTKESACVKDYDLERGPDEKPDEPLHLDLRNVPQSLPWTRRVYEF YSAPFVKFWFYTMAYLAFLMLFTYTVLVEMQPQPSVHEWLVIIYIFTNAIEKVREICISEPSKFKQKVKM WLSEYWNLMETVAIGLFAVGFGLRWGHPPLQTAGRLIYCIDIIFWFSRLMDFFAVNQHAGPYVTMIAKMA ANMFYIVIIMAIVLLSFGVARKAILSPKEPPSWRLARDIVFEPYWMMYGEVYASDIDVCSNETSCPPGSF LTPFLQAVYLFVQYIIMVNLLIACFNNIYLDIKSISNKLWKYNRYRYIMTYHQKPWLPPPFILLNHLCLL LRGLCCRPAPQDQEEGDGGLKLYLTKDDLKKLHDFEEQCVEKYFHEKTEGLNCSFEEQIRMTSERVSEMF FQLKEMNEKVSFIKDSLLSLDSQVGHLQDLSAITVDTLKVLSAVDTLQEDEILLANRKHSTCRKRPHSWT NVICAKVLSDMESCGKKKLQYYSMPPSLLRSLARSQLPPSVQRGALVEVTHSKREASHVREEQEEREMEQ RTTASGISHVRQAHSKYGQFLLVPSSGKQVPLSLETPPHLFRSSEEAGIDGLVLEHIHQSDLTTHLPQQT PAASHQALVAEHKDQHEAVTQMSDKPAKAEQDLLAFSGTPAPMTVTSLPSRAISMQDEGGYVNWAFSEND ETGVFSFKKKWKTCLASTCNSDSNPGGDYFLHTGGRSGLDNSRRLAQSCECPAGPWTQARRSFWINPLCR DKALIKSHSFRFHKEEKLRKTWKNNSHSKSLETRSTWLKAKLLTKTRSLSKKKRKTQGLQVPVITVNACY QSDQLNAEPGETNTTEEFSKKWLSVSNFSQIGLEPYIYQKMKMKEIKRHTTQASDHLRQPQENRDKTPTW NSGSTSLSRSFLTRSPNEVHKISTSLKSPQEPHHHYSAIERNNLMRLSQTIPFTPIQLFTGEEVTIYKLE ESSPLTLDKSMSSWSQHGRAAMIQVLSQEEMDGGLRKAMRVISTWSEDDVLKPGQVFIVKSFLPEVVQTW YKIFQESTVLHLCLREIQQQRAAQKLIYTFNQVKPQTIPYTPRFLEVSLVYCHSANQWLTIEKYMTGEFR KYNNNNGDEIAPTNTLEELMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKPEDKQSRGMVFGPANL GEDAIRSFIAKHRCNSCCGKLRLPDLKRNDYSLSRTHCNLGFGQTIEPTEELPERDKNRSSLEDHTRL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153417 |
| ORF Size | 6084 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_153417.2 |
| RefSeq Size | 6520 bp |
| RefSeq ORF | 6087 bp |
| Locus ID | 225997 |
| UniProt ID | Q8CIR4 |
| Gene Summary | Essential ion channel and kinase. Crucial for magnesium homeostasis. Has an important role in epithelial magnesium transport and in the active magnesium absorption in the gut and kidney (By similarity).[UniProtKB/Swiss-Prot Function] |
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