Trpm7 (NM_001164325) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2310022G15Rik; 4833414K03Rik; 5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224982 representing NM_001164325 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCAGAAATCCTGGATAGAGAGCACTTTGACCAAGAGGGAGTGTGTATATATTATACCAAGCTCCA AAGACCCTCACAGATGTCTTCCAGGATGTCAGATTTGTCAGCAACTTGTCAGATGTTTCTGTGGTCGTTT GGTCAAGCAACATGCATGCTTTACTGCAAGTCTTGCCATGAAATACTCAGATGTGAAATTGGGTGAACAC TTTAACCAGGCAATAGAAGAATGGTCTGTGGAAAAGCACACGGAGCAGAGCCCAACAGATGCTTATGGAG TCATCAATTTTCAAGGGGGTTCTCATTCCTACAGAGCTAAGTATGTGAGACTATCATATGATACCAAACC TGAAATCATTCTGCAACTTCTGCTTAAAGAATGGCAAATGGAGTTACCCAAACTTGTTATTTCTGTACAT GGAGGCATGCAGAAGTTTGAACTTCATCCAAGAATCAAGCAGTTGCTTGGAAAGGGTCTTATTAAAGCTG CAGTTACAACCGGAGCTTGGATTTTAACTGGAGGAGTCAATACAGGTGTGGCAAAACATGTTGGTGATGC CCTCAAAGAACATGCTTCCAGATCATCTCGAAAAATTTGCACTATTGGAATAGCTCCATGGGGAGTGATA GAAAACAGAAATGATCTTGTTGGGAGAGATGTGGTTGCTCCTTATCAAACCCTATTGAATCCCTTGAGCA AATTGAATGTTCTGAATAATCTACACTCCCATTTCATCTTGGTGGATGATGGCACTGTTGGAAAGTATGG GGCAGAAGTCAGACTGAGAAGAGAACTTGAAAAAACCATTAATCAGCAAAGAATTCATGCTAGAATTGGG CAAGGAGTTCCTGTGGTGGCTTTGATATTTGAAGGCGGGCCAAATGTCATCCTTACAGTACTGGAGTACC TTCAGGAAAGCCCCCCAGTTCCAGTTGTTGTGTGTGAAGGGACAGGCAGAGCTGCAGATTTACTAGCCTA TATCCACAAACAGACAGAGGAAGGAGGAAATCTTCCTGATGCAGCAGAGCCTGATATTATATCAACTATC AAGAAAACATTTAACTTTGGCCAGAGTGAAGCAGTTCATTTATTTCAAACAATGATGGAGTGTATGAAAA AAAAAGAGCTTATCACTGTTTTTCACATTGGATCAGAGGATCATCAAGATATAGATGTGGCCATACTCAC TGCACTGCTGAAAGGTACTAATGCATCTGCATTTGACCAGCTTATCCTTACACTGGCATGGGACAGAGTT GATATTGCCAAAAATCATGTATTTGTTTATGGACAACAGTGGCTGGTTGGATCCTTGGAACAGGCTATGC TTGATGCTCTTGTAATGGACAGAGTTTCATTTGTAAAACTTCTTATTGAAAACGGAGTAAGCATGCATAA ATTCCTTACCATTCCCAGACTGGAAGAACTTTATAACACTAAACAAGGTCCAACCAATCCAATGTTGTTC CATCTCATTCGGGATGTCAAGCAGGGTAATCTCCCCCCGGGGTACAAGATCACTTTAATTGATATAGGAC TTGTGATTGAGTATCTCATGGGAGGAACCTACAGATGCACATACACACGAAAACGTTTTCGATTGATATA TAATAGTCTTGGTGGAAATAACCGGAGGTCAGGTCGAAATACCTCCAGCAGCACCCCTCAGTTGCGAAAG AGTCATGAAACTTTTGGCAATAGAGCTGATAAAAAGGAAAAAATGAGACACAATCATTTCATTAAAACAG CCCAACCCTACAGACCAAAGATGGATGCATCTATGGAAGAAGGAAAGAAGAAAAGAACCAAAGATGAAAT TGTAGATATAGATGATCCAGAGACCAAGCGCTTTCCTTATCCTCTTAATGAATTATTAATTTGGGCTTGC CTTATGAAGAGGCAGGTCATGGCCCGCTTTTTATGGCAGCATGGTGAAGAATCAATGGCTAAAGCATTAG TTGCCTGTAAAATCTATCGTTCAATGGCTTATGAGGCAAAGCAGAGTGACCTGGTAGATGATACTTCAGA GGAACTGAAGCAGTATTCCAATGATTTTGGCCAACTGGCAGTTGAATTACTGGAACAGTCCTTCAGACAG GATGAAACGATGGCTATGAAATTACTCACTTATGAACTCAAAAACTGGAGTAATTCAACCTGCCTCAAGT TAGCAGTTTCTTCAAGACTTAGACCTTTTGTAGCTCACACTTGTACACAGATGTTGTTATCTGATATGTG GATGGGACGGCTGAATATGAGAAAAAATTCCTGGTATAAGGTCATATTAAGCATTTTAGTTCCACCTGCC ATATTAATGCTAGAGTATAAAACCAAGGCTGAAATGTCCCATATCCCACAATCTCAAGATGCTCATCAAA TGACGATGGAGGATAGTGAAAACAATTTTCACAACATAACAGAAGAGATACCCATGGAAGTATTTAAAGA AGTAAAGATTTTGGACAGCAGTGATGGAAAGAATGAAATGGAGATACATATTAAATCAAAAAAGCTTCCA ATCACACGAAAATTTTATGCCTTTTATCATGCACCAATTGTAAAGTTCTGGTTTAACACATTGGCATATT TAGGATTTCTGATGCTTTATACATTTGTAGTTCTTGTAAAAATGGAACAGTTACCTTCAGTTCAAGAATG GATTGTTATCGCTTATATTTTTACCTATGCTATTGAAAAAGTCCGTGAGGTCTTCATGTCTGAAGCTGGG AAAATCAGCCAGAAGATTAAAGTATGGTTTAGTGACTACTTCAATGTCAGTGACACAATTGCCATCATTT CTTTCTTTGTTGGATTTGGACTAAGATTTGGAGCAAAATGGAACTATATTAATGCATATGATAATCATGT TTTTGTGGCTGGAAGATTAATTTACTGTCTTAATATAATATTTTGGTATGTGCGTTTGCTAGACTTTCTA GCCGTAAATCAACAGGCAGGACCTTATGTAATGATGATTGGAAAAATGGTGGCCAATATGTTCTACATTG TAGTGATAATGGCTCTTGTATTGCTTAGTTTTGGTGTTCCCAGAAAAGCAATACTTTATCCACATGAAGA ACCATCTTGGTCTCTTGCTAAAGATATAGTTTTTCATCCATACTGGATGATTTTTGGTGAAGTTTATGCA TATGAAATTGATGTGTGTGCAAATGACTCCACTCTCCCGACAATCTGTGGTCCTGGAACTTGGTTGACTC CATTTCTTCAAGCAGTCTACCTCTTTGTACAGTATATCATTATGGTTAATCTCCTTATCGCATTTTTCAA TAATGTATATTTACAAGTGAAGGCAATTTCCAATATTGTATGGAAGTATCAGCGGTATCATTTTATTATG GCTTATCATGAAAAACCAGTCCTGCCTCCTCCTCTTATCATCCTCAGCCATATAGTTTCACTGTTTTGCT GTGTATGCAAAAGAAGAAAGAAAGATAAGACTTCCGATGGGCCAAAACTTTTCTTAACAGAAGAAGATCA AAAGAAACTCCATGATTTTGAAGAGCAGTGTGTTGAGATGTACTTTGATGAGAAAGATGACAAATTCAAT TCTGGGAGTGAAGAGAGAATCCGGGTCACTTTTGAAAGAGTGGAGCAGATGAGCATTCAGATTAAAGAAG TTGGAGATCGTGTCAACTACATAAAAAGATCATTACAGTCTTTAGATTCTCAAATTGGTCATCTGCAAGA TCTCTCAGCCCTAACAGTAGATACATTGAAAACACTTACAGCCCAGAAAGCTTCAGAAGCTAGTAAAGTG CACAATGAGATCACACGAGAATTGAGTATTTCCAAACACTTGGCTCAGAATCTTATTGATGATGTTCCTG TAAGACCTTTGTGGAAGAAACCTAGTGCTGTAAACACACTGAGTTCCTCTCTTCCTCAAGGTGATCGGGA AAGTAATAATCCTTTTCTTTGTAATATTTTTATGAAAGATGAAAAAGACCCCCAATATAATCTGTTTGGA CAAGATTTGCCCGTGATACCCCAGAGAAAAGAATTCAACATTCCAGAGGCTGGTTCCTCCTGTGGTGCCT TATTCCCAAGTGCTGTTTCTCCCCCAGAATTACGACAGAGACGACATGGGGTAGAAATGTTAAAAATATT TAATAAAAATCAAAAATTAGGCAGTTCACCTAATAGTTCACCACATATGTCCTCCCCACCAACCAAATTT TCTGTGAGTACCCCATCCCAGCCAAGTTGCAAAAGTCACTTGGAATCCACAACCAAAGATCAAGAACCCA TTTTCTATAAAGCTGCAGAAGGGGATAACATAGAATTTGGAGCATTTGTGGGACACAGAGATAGTATGGA CTTACAGAGGTTTAAAGAAACATCAAACAAAATAAGAGAACTGTTATCTAATGATACTCCTGAAAACACT CTGAAACATGTGGGTGCTGCTGGATATAGTGAATGTTGTAAGACTTCTACTTCTCTTCACTCGGTGCAAG AAAGCTGTAGTAGAAGAGCGTCGACGGAAGACTCTCCAGAAGTCGATTCTAAAGCAGCTTTGTTACCGGA TTGGTTACGAGATAGACCATCAAACAGAGAAATGCCATCTGAAGGAGGAACATTAAATGGTCTTGCTTCT CCATTTAAGCCCGTTTTGGATACAAATTACTATTATTCAGCTGTGGAAAGAAATAACCTGATGAGGTTGT CACAGAGTATTCCCTTCGTTCCTGTACCTCCACGAGGCGAGCCTGTCACAGTGTACCGTCTGGAGGAGAG TTCTCCCAGTATACTGAATAACAGCATGTCTTCATGGTCTCAGCTAGGCCTCTGTGCCAAAATTGAGTTT TTAAGTAAAGAGGAAATGGGAGGTGGTTTACGAAGAGCAGTCAAAGTGCTGTGTACCTGGTCAGAGCACG ATATCCTGAAGTCAGGGCATCTCTATATCATTAAGTCATTTCTTCCTGAGGTGATAAACACATGGTCAAG CATTTATAAAGAAGATACGGTTCTACATCTCTGTCTCAGAGAAATACAACAACAGAGAGCAGCACAAAAG CTCACATTTGCCTTTAATCAGATGAAACCCAAATCCATACCATATTCTCCAAGGTTCCTTGAAGTTTTCC TGTTGTACTGCCATTCAGCAGGGCAGTGGTTTGCTGTAGAAGAGTGCATGACTGGTGAATTTAGAAAATA CAACAACAATAATGGTGATGAAATCATTCCTACAAATACTCTAGAAGAGATCATGCTAGCCTTTAGCCAC TGGACCTATGAATATACCAGAGGGGAGTTACTGGTACTTGACTTACAAGGAGTGGGAGAAAACTTGACTG ACCCATCTGTAATAAAAGCTGAAGAAAAAAGATCCTGTGACATGGTTTTTGGCCCTGCCAATCTAGGAGA AGATGCAATAAAAAACTTCAGAGCCAAACATCACTGTAATTCTTGCTGTCGAAAGCTTAAACTTCCAGAT TTGAAGAGGAATGACTACACGCCTGATAAAATTATATTTCCTCAGGATGAGTCATCAGATTTGAATCTTC AATCTGGAAATTCCACCAAAGAATCAGAAGCAACAAATTCTGTTCGTCTGATGTTATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001164325
Insert Size	5589 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001164325.1, NP_001157797.1
RefSeq Size	7142 bp
RefSeq ORF	5589 bp
Locus ID	58800
UniProt ID	Q923J1
Gene Summary	Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. Involved in TNF-induced necroptosis downstream of MLKL by mediating calcium influx. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a shorter isoform (2), compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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