Trpm7 (NM_001164325) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2310022G15Rik; 4833414K03Rik; 5033407O22Rik; CHAK; CHAK1; Ltpr7; LTrpC-7; Ltrpc7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR223222 representing NM_001164325 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCAGAAATCCTGGATAGAGAGCACTTTGACCAAGAGGGAGTGTGTATATATTATACCAAGCTCCA AAGACCCTCACAGATGTCTTCCAGGATGTCAGATTTGTCAGCAACTTGTCAGATGTTTCTGTGGTCGTTT GGTCAAGCAACATGCATGCTTTACTGCAAGTCTTGCCATGAAATACTCAGATGTGAAATTGGGTGAACAC TTTAACCAGGCAATAGAAGAATGGTCTGTGGAAAAGCACACGGAGCAGAGCCCAACAGATGCTTATGGAG TCATCAATTTTCAAGGGGGTTCTCATTCCTACAGAGCTAAGTATGTGAGACTATCATATGATACCAAACC TGAAATCATTCTGCAACTTCTGCTTAAAGAATGGCAAATGGAGTTACCCAAACTTGTTATTTCTGTACAT GGAGGCATGCAGAAGTTTGAACTTCATCCAAGAATCAAGCAGTTGCTTGGAAAGGGTCTTATTAAAGCTG CAGTTACAACCGGAGCTTGGATTTTAACTGGAGGAGTCAATACAGGTGTGGCAAAACATGTTGGTGATGC CCTCAAAGAACATGCTTCCAGATCATCTCGAAAAATTTGCACTATTGGAATAGCTCCATGGGGAGTGATA GAAAACAGAAATGATCTTGTTGGGAGAGATGTGGTTGCTCCTTATCAAACCCTATTGAATCCCTTGAGCA AATTGAATGTTCTGAATAATCTACACTCCCATTTCATCTTGGTGGATGATGGCACTGTTGGAAAGTATGG GGCAGAAGTCAGACTGAGAAGAGAACTTGAAAAAACCATTAATCAGCAAAGAATTCATGCTAGAATTGGG CAAGGAGTTCCTGTGGTGGCTTTGATATTTGAAGGCGGGCCAAATGTCATCCTTACAGTACTGGAGTACC TTCAGGAAAGCCCCCCAGTTCCAGTTGTTGTGTGTGAAGGGACAGGCAGAGCTGCAGATTTACTAGCCTA TATCCACAAACAGACAGAGGAAGGAGGAAATCTTCCTGATGCAGCAGAGCCTGATATTATATCAACTATC AAGAAAACATTTAACTTTGGCCAGAGTGAAGCAGTTCATTTATTTCAAACAATGATGGAGTGTATGAAAA AAAAAGAGCTTATCACTGTTTTTCACATTGGATCAGAGGATCATCAAGATATAGATGTGGCCATACTCAC TGCACTGCTGAAAGGTACTAATGCATCTGCATTTGACCAGCTTATCCTTACACTGGCATGGGACAGAGTT GATATTGCCAAAAATCATGTATTTGTTTATGGACAACAGTGGCTGGTTGGATCCTTGGAACAGGCTATGC TTGATGCTCTTGTAATGGACAGAGTTTCATTTGTAAAACTTCTTATTGAAAACGGAGTAAGCATGCATAA ATTCCTTACCATTCCCAGACTGGAAGAACTTTATAACACTAAACAAGGTCCAACCAATCCAATGTTGTTC CATCTCATTCGGGATGTCAAGCAGGGTAATCTCCCCCCGGGGTACAAGATCACTTTAATTGATATAGGAC TTGTGATTGAGTATCTCATGGGAGGAACCTACAGATGCACATACACACGAAAACGTTTTCGATTGATATA TAATAGTCTTGGTGGAAATAACCGGAGGTCAGGTCGAAATACCTCCAGCAGCACCCCTCAGTTGCGAAAG AGTCATGAAACTTTTGGCAATAGAGCTGATAAAAAGGAAAAAATGAGACACAATCATTTCATTAAAACAG CCCAACCCTACAGACCAAAGATGGATGCATCTATGGAAGAAGGAAAGAAGAAAAGAACCAAAGATGAAAT TGTAGATATAGATGATCCAGAGACCAAGCGCTTTCCTTATCCTCTTAATGAATTATTAATTTGGGCTTGC CTTATGAAGAGGCAGGTCATGGCCCGCTTTTTATGGCAGCATGGTGAAGAATCAATGGCTAAAGCATTAG TTGCCTGTAAAATCTATCGTTCAATGGCTTATGAGGCAAAGCAGAGTGACCTGGTAGATGATACTTCAGA GGAACTGAAGCAGTATTCCAATGATTTTGGCCAACTGGCAGTTGAATTACTGGAACAGTCCTTCAGACAG GATGAAACGATGGCTATGAAATTACTCACTTATGAACTCAAAAACTGGAGTAATTCAACCTGCCTCAAGT TAGCAGTTTCTTCAAGACTTAGACCTTTTGTAGCTCACACTTGTACACAGATGTTGTTATCTGATATGTG GATGGGACGGCTGAATATGAGAAAAAATTCCTGGTATAAGGTCATATTAAGCATTTTAGTTCCACCTGCC ATATTAATGCTAGAGTATAAAACCAAGGCTGAAATGTCCCATATCCCACAATCTCAAGATGCTCATCAAA TGACGATGGAGGATAGTGAAAACAATTTTCACAACATAACAGAAGAGATACCCATGGAAGTATTTAAAGA AGTAAAGATTTTGGACAGCAGTGATGGAAAGAATGAAATGGAGATACATATTAAATCAAAAAAGCTTCCA ATCACACGAAAATTTTATGCCTTTTATCATGCACCAATTGTAAAGTTCTGGTTTAACACATTGGCATATT TAGGATTTCTGATGCTTTATACATTTGTAGTTCTTGTAAAAATGGAACAGTTACCTTCAGTTCAAGAATG GATTGTTATCGCTTATATTTTTACCTATGCTATTGAAAAAGTCCGTGAGGTCTTCATGTCTGAAGCTGGG AAAATCAGCCAGAAGATTAAAGTATGGTTTAGTGACTACTTCAATGTCAGTGACACAATTGCCATCATTT CTTTCTTTGTTGGATTTGGACTAAGATTTGGAGCAAAATGGAACTATATTAATGCATATGATAATCATGT TTTTGTGGCTGGAAGATTAATTTACTGTCTTAATATAATATTTTGGTATGTGCGTTTGCTAGACTTTCTA GCCGTAAATCAACAGGCAGGACCTTATGTAATGATGATTGGAAAAATGGTGGCCAATATGTTCTACATTG TAGTGATAATGGCTCTTGTATTGCTTAGTTTTGGTGTTCCCAGAAAAGCAATACTTTATCCACATGAAGA ACCATCTTGGTCTCTTGCTAAAGATATAGTTTTTCATCCATACTGGATGATTTTTGGTGAAGTTTATGCA TATGAAATTGATGTGTGTGCAAATGACTCCACTCTCCCGACAATCTGTGGTCCTGGAACTTGGTTGACTC CATTTCTTCAAGCAGTCTACCTCTTTGTACAGTATATCATTATGGTTAATCTCCTTATCGCATTTTTCAA TAATGTATATTTACAAGTGAAGGCAATTTCCAATATTGTATGGAAGTATCAGCGGTATCATTTTATTATG GCTTATCATGAAAAACCAGTCCTGCCTCCTCCTCTTATCATCCTCAGCCATATAGTTTCACTGTTTTGCT GTGTATGCAAAAGAAGAAAGAAAGATAAGACTTCCGATGGGCCAAAACTTTTCTTAACAGAAGAAGATCA AAAGAAACTCCATGATTTTGAAGAGCAGTGTGTTGAGATGTACTTTGATGAGAAAGATGACAAATTCAAT TCTGGGAGTGAAGAGAGAATCCGGGTCACTTTTGAAAGAGTGGAGCAGATGAGCATTCAGATTAAAGAAG TTGGAGATCGTGTCAACTACATAAAAAGATCATTACAGTCTTTAGATTCTCAAATTGGTCATCTGCAAGA TCTCTCAGCCCTAACAGTAGATACATTGAAAACACTTACAGCCCAGAAAGCTTCAGAAGCTAGTAAAGTG CACAATGAGATCACACGAGAATTGAGTATTTCCAAACACTTGGCTCAGAATCTTATTGATGATGTTCCTG TAAGACCTTTGTGGAAGAAACCTAGTGCTGTAAACACACTGAGTTCCTCTCTTCCTCAAGGTGATCGGGA AAGTAATAATCCTTTTCTTTGTAATATTTTTATGAAAGATGAAAAAGACCCCCAATATAATCTGTTTGGA CAAGATTTGCCCGTGATACCCCAGAGAAAAGAATTCAACATTCCAGAGGCTGGTTCCTCCTGTGGTGCCT TATTCCCAAGTGCTGTTTCTCCCCCAGAATTACGACAGAGACGACATGGGGTAGAAATGTTAAAAATATT TAATAAAAATCAAAAATTAGGCAGTTCACCTAATAGTTCACCACATATGTCCTCCCCACCAACCAAATTT TCTGTGAGTACCCCATCCCAGCCAAGTTGCAAAAGTCACTTGGAATCCACAACCAAAGATCAAGAACCCA TTTTCTATAAAGCTGCAGAAGGGGATAACATAGAATTTGGAGCATTTGTGGGACACAGAGATAGTATGGA CTTACAGAGGTTTAAAGAAACATCAAACAAAATAAGAGAACTGTTATCTAATGATACTCCTGAAAACACT CTGAAACATGTGGGTGCTGCTGGATATAGTGAATGTTGTAAGACTTCTACTTCTCTTCACTCGGTGCAAG AAAGCTGTAGTAGAAGAGCGTCGACGGAAGACTCTCCAGAAGTCGATTCTAAAGCAGCTTTGTTACCGGA TTGGTTACGAGATAGACCATCAAACAGAGAAATGCCATCTGAAGGAGGAACATTAAATGGTCTTGCTTCT CCATTTAAGCCCGTTTTGGATACAAATTACTATTATTCAGCTGTGGAAAGAAATAACCTGATGAGGTTGT CACAGAGTATTCCCTTCGTTCCTGTACCTCCACGAGGCGAGCCTGTCACAGTGTACCGTCTGGAGGAGAG TTCTCCCAGTATACTGAATAACAGCATGTCTTCATGGTCTCAGCTAGGCCTCTGTGCCAAAATTGAGTTT TTAAGTAAAGAGGAAATGGGAGGTGGTTTACGAAGAGCAGTCAAAGTGCTGTGTACCTGGTCAGAGCACG ATATCCTGAAGTCAGGGCATCTCTATATCATTAAGTCATTTCTTCCTGAGGTGATAAACACATGGTCAAG CATTTATAAAGAAGATACGGTTCTACATCTCTGTCTCAGAGAAATACAACAACAGAGAGCAGCACAAAAG CTCACATTTGCCTTTAATCAGATGAAACCCAAATCCATACCATATTCTCCAAGGTTCCTTGAAGTTTTCC TGTTGTACTGCCATTCAGCAGGGCAGTGGTTTGCTGTAGAAGAGTGCATGACTGGTGAATTTAGAAAATA CAACAACAATAATGGTGATGAAATCATTCCTACAAATACTCTAGAAGAGATCATGCTAGCCTTTAGCCAC TGGACCTATGAATATACCAGAGGGGAGTTACTGGTACTTGACTTACAAGGAGTGGGAGAAAACTTGACTG ACCCATCTGTAATAAAAGCTGAAGAAAAAAGATCCTGTGACATGGTTTTTGGCCCTGCCAATCTAGGAGA AGATGCAATAAAAAACTTCAGAGCCAAACATCACTGTAATTCTTGCTGTCGAAAGCTTAAACTTCCAGAT TTGAAGAGGAATGACTACACGCCTGATAAAATTATATTTCCTCAGGATGAGTCATCAGATTTGAATCTTC AATCTGGAAATTCCACCAAAGAATCAGAAGCAACAAATTCTGTTCGTCTGATGTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR223222 representing NM_001164325 Red=Cloning site Green=Tags(s) MSQKSWIESTLTKRECVYIIPSSKDPHRCLPGCQICQQLVRCFCGRLVKQHACFTASLAMKYSDVKLGEH FNQAIEEWSVEKHTEQSPTDAYGVINFQGGSHSYRAKYVRLSYDTKPEIILQLLLKEWQMELPKLVISVH GGMQKFELHPRIKQLLGKGLIKAAVTTGAWILTGGVNTGVAKHVGDALKEHASRSSRKICTIGIAPWGVI ENRNDLVGRDVVAPYQTLLNPLSKLNVLNNLHSHFILVDDGTVGKYGAEVRLRRELEKTINQQRIHARIG QGVPVVALIFEGGPNVILTVLEYLQESPPVPVVVCEGTGRAADLLAYIHKQTEEGGNLPDAAEPDIISTI KKTFNFGQSEAVHLFQTMMECMKKKELITVFHIGSEDHQDIDVAILTALLKGTNASAFDQLILTLAWDRV DIAKNHVFVYGQQWLVGSLEQAMLDALVMDRVSFVKLLIENGVSMHKFLTIPRLEELYNTKQGPTNPMLF HLIRDVKQGNLPPGYKITLIDIGLVIEYLMGGTYRCTYTRKRFRLIYNSLGGNNRRSGRNTSSSTPQLRK SHETFGNRADKKEKMRHNHFIKTAQPYRPKMDASMEEGKKKRTKDEIVDIDDPETKRFPYPLNELLIWAC LMKRQVMARFLWQHGEESMAKALVACKIYRSMAYEAKQSDLVDDTSEELKQYSNDFGQLAVELLEQSFRQ DETMAMKLLTYELKNWSNSTCLKLAVSSRLRPFVAHTCTQMLLSDMWMGRLNMRKNSWYKVILSILVPPA ILMLEYKTKAEMSHIPQSQDAHQMTMEDSENNFHNITEEIPMEVFKEVKILDSSDGKNEMEIHIKSKKLP ITRKFYAFYHAPIVKFWFNTLAYLGFLMLYTFVVLVKMEQLPSVQEWIVIAYIFTYAIEKVREVFMSEAG KISQKIKVWFSDYFNVSDTIAIISFFVGFGLRFGAKWNYINAYDNHVFVAGRLIYCLNIIFWYVRLLDFL AVNQQAGPYVMMIGKMVANMFYIVVIMALVLLSFGVPRKAILYPHEEPSWSLAKDIVFHPYWMIFGEVYA YEIDVCANDSTLPTICGPGTWLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLQVKAISNIVWKYQRYHFIM AYHEKPVLPPPLIILSHIVSLFCCVCKRRKKDKTSDGPKLFLTEEDQKKLHDFEEQCVEMYFDEKDDKFN SGSEERIRVTFERVEQMSIQIKEVGDRVNYIKRSLQSLDSQIGHLQDLSALTVDTLKTLTAQKASEASKV HNEITRELSISKHLAQNLIDDVPVRPLWKKPSAVNTLSSSLPQGDRESNNPFLCNIFMKDEKDPQYNLFG QDLPVIPQRKEFNIPEAGSSCGALFPSAVSPPELRQRRHGVEMLKIFNKNQKLGSSPNSSPHMSSPPTKF SVSTPSQPSCKSHLESTTKDQEPIFYKAAEGDNIEFGAFVGHRDSMDLQRFKETSNKIRELLSNDTPENT LKHVGAAGYSECCKTSTSLHSVQESCSRRASTEDSPEVDSKAALLPDWLRDRPSNREMPSEGGTLNGLAS PFKPVLDTNYYYSAVERNNLMRLSQSIPFVPVPPRGEPVTVYRLEESSPSILNNSMSSWSQLGLCAKIEF LSKEEMGGGLRRAVKVLCTWSEHDILKSGHLYIIKSFLPEVINTWSSIYKEDTVLHLCLREIQQQRAAQK LTFAFNQMKPKSIPYSPRFLEVFLLYCHSAGQWFAVEECMTGEFRKYNNNNGDEIIPTNTLEEIMLAFSH WTYEYTRGELLVLDLQGVGENLTDPSVIKAEEKRSCDMVFGPANLGEDAIKNFRAKHHCNSCCRKLKLPD LKRNDYTPDKIIFPQDESSDLNLQSGNSTKESEATNSVRLML TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001164325
ORF Size	5586 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001164325.1, NP_001157797.1
RefSeq Size	7142 bp
RefSeq ORF	5589 bp
Locus ID	58800
UniProt ID	Q923J1
MW	212.8 kDa
Gene Summary	Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. Involved in TNF-induced necroptosis downstream of MLKL by mediating calcium influx. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).[UniProtKB/Swiss-Prot Function]

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