Arfgef2 (NM_001085495) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI463430; AW493672; BIG2; E230011G24Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224934 representing NM_001085495 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGAAAGCCAGACCAAGAGCATGTTCGTGTCCCGGGCCCTGGAGAAGATCCTAGCTGACAAGGAGG TGAAGCGGCCCCAGCACTCCCAGCTGCGTAGGGCCTGCCAGGTGGCGCTCGATGAAATTAAAGCAGAACT AGAAAAGCAGAGGCTTGGCGCTGCGGCTCCACCAAAGGCAAACTTCATTGAAGCTGACAAGTATTTCCTT CCATTCGAGCTGGCCTGCCAATCCAAGTCACCTCGGGTGGTCAGCACATCCCTGGACTGCTTGCAGAAAC TCATCGCATATGGACACATCACTGGCAATGCCCCTGACAGTGGCGCTCCTGGGAAGCGGCTGATCGACAG GATCGTTGAAACCATTTGCAATTGTTTTCAGGGCCCTCAGACAGATGAAGGGGTTCAGCTACAAATCATT AAGGCTCTGCTGACTGCTGTGACATCCCCACACATTGAAATCCATGAGGGCACCATCCTGCAGACAGTGA GGACCTGCTACAATATCTATCTGGCCAGCAAAAACCTCATCAATCAAACCACCGCCAAGGCCACCCTTAC CCAAATGCTGAATGTCATCTTCACCCGCATGGAAAACCAAGTGTTACAGGAGGCCAGAGAATTGGAAAAA CCAATGCAGTCAAAACCTCAGTCCCCGGTGATCCAGGCCACAGCAGGCTCTCCCAAGTTCAGCCGCCTGA AGCAGAGCCAGGCCCAGAGCAAACCCACGACTCCTGAGAAAGCAGAGCTGCCCAATGGGGACCATGCCCA GAGTGGCCTAGGAAAAGTGAGCTTGGAAAATGGAGAAGCTCCCCGAGAGAGAGGCTCGCCAGTCTCTGGG AGAGCCGAGCCTTCGAGGGGCACTGACAGTGGAGCTCAGGAGGTGGTGAAGGACATCTTAGAGGACGTGG TCACGTCTGCTGTGAAAGAAGCAGCAGAAAAGCATGGTCTGCCGGAGCCCGACCGAGCCCTGGGTGCCCT AGAGTGCCAGGAATGCGCTGTTCCTCCTGGAGTTGATGAAAACTCACAGACCAACGGCATAGCAGACGAC AGGCAGTCGCTATCCTCCGCAGATAATCTGGAACCAGATGTTCAAGGACACCAAGTGGCTGCTAGGTTCT CTCACATTCTACAGAAGGATGCCTTCCTGGTGTTCCGCTCCCTGTGCAAGCTGTCCATGAAGCCCCTTGG GGAAGGGCCACCAGATCCAAAGTCCCACGAGCTTCGTTCCAAGGTGGTTTCCCTGCAGCTGCTCCTCTCT GTGCTGCAGAATGCTGGCCCAGTCTTCAGGAGCCACGAGATGTTCGTCACTGCCATTAAGCAGTACCTGT GTGTGGCCCTTTCCAAGAATGGCGTGTCCTCAGTGCCTGACGTCTTCGAACTCTCGCTTGCTATTTTTCT TACTCTTCTTTCAAACTTCAAGATGCACTTGAAAATGCAGATAGAGGTCTTTTTCAAAGAGATTTTCCTG AACATTTTAGAAACATCCACAAGTTCTTTTGAGCACCGCTGGATGGTCATTCAGACCCTGACGAGGATTT GTGCAGATGCCCAGTGCGTTGTGGATATTTATGTCAACTACGACTGTGACTTAAATGCTGCTAACATTTT CGAGCGACTAGTAAATGACTTATCCAAAATTGCTCAGGGAAGAAGTGGACATGAGCTGGGGATGACACCT CTACAGGAGCTCAGTCTGAGGAAGAAAGGCCTGGAGTGTCTGGTGTCTATTCTCAAGTGCATGGTGGAGT GGAGCAAGGACCTGTATGTGAATCCCAACCACCAGGCTACCCTGGGTCAGGAGAGGCTCCCAGATCAGGA AATGGGGGACGGAAAAGGCCTTGACATGGCAAGACGGTGCAGCGTGACATCCGTGGAGTCCACAGTGTCC TCGGGGACCCAGACAGCCATTCAGGATGACCCAGAGCAGTTTGAGGTCATCAAACAACAGAAGGAAATCA TTGAGCATGGCATTGAGCTCTTCAATAAGAAGCCCAAGCGAGGTATCCAGTTTCTCCAAGAGCAAGGCAT GCTGGGAGCAGCTGTAGAAGATATTGCCCAATTTCTGCACCAGGAGGAGCGGCTGGATTCTACCCAGGTT GGCGAGTTTCTGGGAGACAGCACGAGGTTTAACAAGGAGGTGATGTATGCCTATGTGGACCAACTGGATT TCTGTGAAAAAGAGTTCGTCTCAGCCCTGAGGACATTCCTGGAGGGCTTCCGCCTGCCTGGGGAAGCCCA GAAGATTGACCGGTTAATGGAGAAGTTTGCTGCACGATACATAGAATGTAACCAAGGGCAAACCCTGTTT GCTAGTGCGGACACTGCGTATGTCCTGGCGTACTCCATCATCATGCTGACCACAGACCTGCACAGCCCAC AGGTAAAGAATAAGATGACAAAAGAGCAGTATATTAAAATGAACCGGGGCATCAACGACAGCAAAGACCT GCCTGAGGAGTACCTGTCCAGCATCTATGACGAGATAGAGGGCAAGAAGATAGCGATGAAGGAGACAAAG GAGCACACAATTGCGACCAAGTCCACCAAGCAGAGTGTAGCTAGTGAAAAGCAGAGGCGGCTGCTGTACA ATGTGGAGATGGAGCAGATGGCTAAAACAGCCAAAGCTCTGATGGAGGCCGTGAGCCATGCCAAAGCCCC GTTTACCAGCGCCACACACTTGGACCATGTCCGGCCAATGTTCAAACTGGTGTGGACGCCGCTGCTGGCA GCCTATAGTATTGGCCTGCAGAACTGTGATGACACGGAGGTGGCCTCCCTGTGTTTGGAAGGCATCAGGT GTGCAGTCCGCATTGCCTGCATCTTTGGAATGCAGCTGGAACGAGATGCCTATGTTCAGGCCCTTGCTCG CTTTTCCCTGCTGACAGCCAGCTCCAGCATCACAGAAATGAAGCAGAAAAACATCGACACCATAAAGACA CTCATCACTGTGGCTCACACCGATGGCAACTACCTTGGCAACTCCTGGCATGAGATCTTGAAGTGCATCA GCCAGCTGGAGCTTGCTCAGCTGATAGGAACTGGGGTGAAGACTCGCTACCTCTCTGGCTCTGGGCGGGA ACGAGAAGGGAGCCTGAAGGGCCACAGTCTGGCAGGAGAAGAATTCATGGGTCTTGGCCTCGGTAACTTG GTGAGTGGTGGTGTAGACAAAAGACAGATGGCCAGCTTCCAGGAGTCGGTTGGTGAAACAAGCTCACAGA GTGTGGTTGTCGCAGTTGACAGAATTTTTACTGGCTCTACCAGACTGGATGGAAATGCAATAGTTGACTT TGTCCGTTGGCTGTGTGCTGTGTCCATGGATGAGCTGGCTTCTCCCCACCATCCTCGAATGTTCAGCCTG CAGAAGATTGTGGAAATATCATACTACAACATGAACAGGATCCGGCTGCAGTGGTCTCGGATATGGCATG TCATTGGAGATCACTTCAATAAGGTCGGCTGTAACCCCAATGAAGATGTGGCCATCTTCGCAGTTGACTC TCTGCGGCAGCTCTCCATGAAGTTCCTGGAGAAGGGAGAATTAGCCAACTTCCGATTTCAGAAGGATTTT CTGAGGCCATTTGAACATATTATGAAGAAAAACAGGTCCCCGACCATCCGGGACATGGTGATCCGATGCA TCGCTCAGATGGTGAGCTCCCAGGCAGCCAACATCCGCTCAGGCTGGAAGAACATCTTTGCTGTGTTCCA CCAGGCTGCCTCTGACCACGATGGGAACATTGTGGAGCTGGCCTTCCAAACCACAGGCCACATCGTCTCA ACCATCTTCCAGCACCACTTCCCTGCAGCCATCGACTCCTTCCAGGATGCTGTGAAGTGCTTGTCTGAGT TCGCCTGCAATGCAGCTTTCCCTGACACCAGCATGGAGGCCATCCGGCTCATCCGCTTCTGTGGGAAATA CGTCTCAGAGAGGCCTCGGGTGCTGCAAGAGTACACAAGTGATGATATGAATGTGGCTCCTGGTGACAGG GTCTGGGTCAGAGGCTGGTTTCCCATCCTGTTTGAACTCTCCTGCATCATTAACAGATGCAAGTTAGATG TACGCACAAGGGGACTCACGGTCATGTTTGAGATTATGAAGAGCTATGGCCACACCTTTGCAAAGCACTG GTGGCAGGACCTGTTCAGAATCGTGTTTCGAATTTTTGACAACATGAAACTCCCTGAGCAACAGTCAGAG AAATCAGAGTGGATGACGACCACGTGCAATCATGCACTGTACGCTATTTGTGACGTATTCACCCAGTTCT ACGAAGCTTTGCATGAAGTGCTTCTCTCTGATGTGTTTGCGCAGCTGCAGTGGTGCGTGAAACAAGATAA TGAGCAGTTGGCCCGATCAGGTACGAACTGCTTAGAGAACCTAGTGATATCCAACGGCGAGAAGTTCAGC CCTGCTGTCTGGGATGAAACCTGCAATTGCATGTTGGATATTTTCAAAACCACCATCCCACATGTTTTGC TGACGTGGAGACCTGCGGGGATGGAGGAGGAGGTGTCAGATAGACATCTGGATGTGGACCTAGACCGCCA GTCTTTAAGCAGCATAGACAGAAACGCCTCCGAGAGAGGACAGAGCCAGCTGTCCAACCCCACTGACGAC AGCTGGAAGGGGGCACCGTATGCACATCAGAAACTGCTGGCCAGCCTCCTCATCAAGTGTGTGGTTCAGC TGGAGCTGATCCAGACCATTGACAACATCGTGTTCTACCCTGCCACCAGCAAGAAGGAGGATGCGGAGCA CATGGTTGCTGCCCAGCAAGACACCCTCGATGCAGAGATCCACATCGAGACGGAGAATCAGGGCATGTAC AAGTTCATGTCCTCCCAGCACCTCTTCAAGCTGCTGGACTGCCTGCAGGAGTCCCACTCCTTCTCCAAAG CCTTCAACTCCAACTATGAGCAGCGGACTGTCCTCTGGCGAGCTGGCTTCAAGGGCAAGTCCAAACCCAA TCTTCTCAAACAAGAAACCAGCAGCCTGGCCTGTTGTCTAAGGATCCTGTTTAGAATGTACGTTGATGAG AACCGCAGGGATTCCTGGGACGAAATACAGCAGCGACTTCTAAGAGTGTGCAGTGAAGCACTTGCCTATT TCATTACTGTGAACTCCGAGAGCCATCGGGAAGCATGGACGAGCCTCCTGCTGCTACTTCTAACCAAAAC CCTCAAGATAAGCGATGAAAAGTTCAAAGCACACGCGTCAATGTACTACCCCTACCTGTGTGAAATTATG CAGTTTGACCTGATCCCTGAGCTCCGAGCAGTTCTGCGAAAGTTCTTCCTGCGGATAGGCCTGGTGTATA AGATATGGATTCCAGAAGAGCCGTCGCAAGTGCCAGCAGCACTGTCATCGACCTGGTAG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001085495
Insert Size	5379 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001085495.2, NP_001078964.1
RefSeq Size	8777 bp
RefSeq ORF	5379 bp
Locus ID	99371
UniProt ID	A2A5R2
Gene Summary	Promotes guanine-nucleotide exchange on ARF1 and ARF3 and to a lower extent on ARF5 and ARF6. Promotes the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP. Involved in the regulation of Golgi vesicular transport. Required for the integrity of the endosomal compartment. Involved in trafficking from the trans-Golgi network (TGN) to endosomes and is required for membrane association of the AP-1 complex and GGA1. Seems to be involved in recycling of the transferrin receptor from recycling endosomes to the plasma membrane. Probably is involved in the exit of GABA(A) receptors from the endoplasmic reticulum. Involved in constitutive release of tumor necrosis factor receptor 1 via exosome-like vesicles; the function seems to involve PKA and specifically PRKAR2B. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways (By similarity).[UniProtKB/Swiss-Prot Function]

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