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Arfgef2 (NM_001085495) Mouse Tagged ORF Clone
CAT＃: MG213940
- TrueORF®
Arfgef2 (tGFP-tagged) - Mouse ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (Arfgef2), (10ug)
ORF Plasmid: DDK
"NM_001085495" in other vectors (2)
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CNY 19,670.00
货期*
9周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI463430; AW493672; BIG2; E230011G24Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG213940 representing NM_001085495
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGAAAGCCAGACCAAGAGCATGTTCGTGTCCCGGGCCCTGGAGAAGATCCTAGCTGACAAGGAGG TGAAGCGGCCCCAGCACTCCCAGCTGCGTAGGGCCTGCCAGGTGGCGCTCGATGAAATTAAAGCAGAACT AGAAAAGCAGAGGCTTGGCGCTGCGGCTCCACCAAAGGCAAACTTCATTGAAGCTGACAAGTATTTCCTT CCATTCGAGCTGGCCTGCCAATCCAAGTCACCTCGGGTGGTCAGCACATCCCTGGACTGCTTGCAGAAAC TCATCGCATATGGACACATCACTGGCAATGCCCCTGACAGTGGCGCTCCTGGGAAGCGGCTGATCGACAG GATCGTTGAAACCATTTGCAATTGTTTTCAGGGCCCTCAGACAGATGAAGGGGTTCAGCTACAAATCATT AAGGCTCTGCTGACTGCTGTGACATCCCCACACATTGAAATCCATGAGGGCACCATCCTGCAGACAGTGA GGACCTGCTACAATATCTATCTGGCCAGCAAAAACCTCATCAATCAAACCACCGCCAAGGCCACCCTTAC CCAAATGCTGAATGTCATCTTCACCCGCATGGAAAACCAAGTGTTACAGGAGGCCAGAGAATTGGAAAAA CCAATGCAGTCAAAACCTCAGTCCCCGGTGATCCAGGCCACAGCAGGCTCTCCCAAGTTCAGCCGCCTGA AGCAGAGCCAGGCCCAGAGCAAACCCACGACTCCTGAGAAAGCAGAGCTGCCCAATGGGGACCATGCCCA GAGTGGCCTAGGAAAAGTGAGCTTGGAAAATGGAGAAGCTCCCCGAGAGAGAGGCTCGCCAGTCTCTGGG AGAGCCGAGCCTTCGAGGGGCACTGACAGTGGAGCTCAGGAGGTGGTGAAGGACATCTTAGAGGACGTGG TCACGTCTGCTGTGAAAGAAGCAGCAGAAAAGCATGGTCTGCCGGAGCCCGACCGAGCCCTGGGTGCCCT AGAGTGCCAGGAATGCGCTGTTCCTCCTGGAGTTGATGAAAACTCACAGACCAACGGCATAGCAGACGAC AGGCAGTCGCTATCCTCCGCAGATAATCTGGAACCAGATGTTCAAGGACACCAAGTGGCTGCTAGGTTCT CTCACATTCTACAGAAGGATGCCTTCCTGGTGTTCCGCTCCCTGTGCAAGCTGTCCATGAAGCCCCTTGG GGAAGGGCCACCAGATCCAAAGTCCCACGAGCTTCGTTCCAAGGTGGTTTCCCTGCAGCTGCTCCTCTCT GTGCTGCAGAATGCTGGCCCAGTCTTCAGGAGCCACGAGATGTTCGTCACTGCCATTAAGCAGTACCTGT GTGTGGCCCTTTCCAAGAATGGCGTGTCCTCAGTGCCTGACGTCTTCGAACTCTCGCTTGCTATTTTTCT TACTCTTCTTTCAAACTTCAAGATGCACTTGAAAATGCAGATAGAGGTCTTTTTCAAAGAGATTTTCCTG AACATTTTAGAAACATCCACAAGTTCTTTTGAGCACCGCTGGATGGTCATTCAGACCCTGACGAGGATTT GTGCAGATGCCCAGTGCGTTGTGGATATTTATGTCAACTACGACTGTGACTTAAATGCTGCTAACATTTT CGAGCGACTAGTAAATGACTTATCCAAAATTGCTCAGGGAAGAAGTGGACATGAGCTGGGGATGACACCT CTACAGGAGCTCAGTCTGAGGAAGAAAGGCCTGGAGTGTCTGGTGTCTATTCTCAAGTGCATGGTGGAGT GGAGCAAGGACCTGTATGTGAATCCCAACCACCAGGCTACCCTGGGTCAGGAGAGGCTCCCAGATCAGGA AATGGGGGACGGAAAAGGCCTTGACATGGCAAGACGGTGCAGCGTGACATCCGTGGAGTCCACAGTGTCC TCGGGGACCCAGACAGCCATTCAGGATGACCCAGAGCAGTTTGAGGTCATCAAACAACAGAAGGAAATCA TTGAGCATGGCATTGAGCTCTTCAATAAGAAGCCCAAGCGAGGTATCCAGTTTCTCCAAGAGCAAGGCAT GCTGGGAGCAGCTGTAGAAGATATTGCCCAATTTCTGCACCAGGAGGAGCGGCTGGATTCTACCCAGGTT GGCGAGTTTCTGGGAGACAGCACGAGGTTTAACAAGGAGGTGATGTATGCCTATGTGGACCAACTGGATT TCTGTGAAAAAGAGTTCGTCTCAGCCCTGAGGACATTCCTGGAGGGCTTCCGCCTGCCTGGGGAAGCCCA GAAGATTGACCGGTTAATGGAGAAGTTTGCTGCACGATACATAGAATGTAACCAAGGGCAAACCCTGTTT GCTAGTGCGGACACTGCGTATGTCCTGGCGTACTCCATCATCATGCTGACCACAGACCTGCACAGCCCAC AGGTAAAGAATAAGATGACAAAAGAGCAGTATATTAAAATGAACCGGGGCATCAACGACAGCAAAGACCT GCCTGAGGAGTACCTGTCCAGCATCTATGACGAGATAGAGGGCAAGAAGATAGCGATGAAGGAGACAAAG GAGCACACAATTGCGACCAAGTCCACCAAGCAGAGTGTAGCTAGTGAAAAGCAGAGGCGGCTGCTGTACA ATGTGGAGATGGAGCAGATGGCTAAAACAGCCAAAGCTCTGATGGAGGCCGTGAGCCATGCCAAAGCCCC GTTTACCAGCGCCACACACTTGGACCATGTCCGGCCAATGTTCAAACTGGTGTGGACGCCGCTGCTGGCA GCCTATAGTATTGGCCTGCAGAACTGTGATGACACGGAGGTGGCCTCCCTGTGTTTGGAAGGCATCAGGT GTGCAGTCCGCATTGCCTGCATCTTTGGAATGCAGCTGGAACGAGATGCCTATGTTCAGGCCCTTGCTCG CTTTTCCCTGCTGACAGCCAGCTCCAGCATCACAGAAATGAAGCAGAAAAACATCGACACCATAAAGACA CTCATCACTGTGGCTCACACCGATGGCAACTACCTTGGCAACTCCTGGCATGAGATCTTGAAGTGCATCA GCCAGCTGGAGCTTGCTCAGCTGATAGGAACTGGGGTGAAGACTCGCTACCTCTCTGGCTCTGGGCGGGA ACGAGAAGGGAGCCTGAAGGGCCACAGTCTGGCAGGAGAAGAATTCATGGGTCTTGGCCTCGGTAACTTG GTGAGTGGTGGTGTAGACAAAAGACAGATGGCCAGCTTCCAGGAGTCGGTTGGTGAAACAAGCTCACAGA GTGTGGTTGTCGCAGTTGACAGAATTTTTACTGGCTCTACCAGACTGGATGGAAATGCAATAGTTGACTT TGTCCGTTGGCTGTGTGCTGTGTCCATGGATGAGCTGGCTTCTCCCCACCATCCTCGAATGTTCAGCCTG CAGAAGATTGTGGAAATATCATACTACAACATGAACAGGATCCGGCTGCAGTGGTCTCGGATATGGCATG TCATTGGAGATCACTTCAATAAGGTCGGCTGTAACCCCAATGAAGATGTGGCCATCTTCGCAGTTGACTC TCTGCGGCAGCTCTCCATGAAGTTCCTGGAGAAGGGAGAATTAGCCAACTTCCGATTTCAGAAGGATTTT CTGAGGCCATTTGAACATATTATGAAGAAAAACAGGTCCCCGACCATCCGGGACATGGTGATCCGATGCA TCGCTCAGATGGTGAGCTCCCAGGCAGCCAACATCCGCTCAGGCTGGAAGAACATCTTTGCTGTGTTCCA CCAGGCTGCCTCTGACCACGATGGGAACATTGTGGAGCTGGCCTTCCAAACCACAGGCCACATCGTCTCA ACCATCTTCCAGCACCACTTCCCTGCAGCCATCGACTCCTTCCAGGATGCTGTGAAGTGCTTGTCTGAGT TCGCCTGCAATGCAGCTTTCCCTGACACCAGCATGGAGGCCATCCGGCTCATCCGCTTCTGTGGGAAATA CGTCTCAGAGAGGCCTCGGGTGCTGCAAGAGTACACAAGTGATGATATGAATGTGGCTCCTGGTGACAGG GTCTGGGTCAGAGGCTGGTTTCCCATCCTGTTTGAACTCTCCTGCATCATTAACAGATGCAAGTTAGATG TACGCACAAGGGGACTCACGGTCATGTTTGAGATTATGAAGAGCTATGGCCACACCTTTGCAAAGCACTG GTGGCAGGACCTGTTCAGAATCGTGTTTCGAATTTTTGACAACATGAAACTCCCTGAGCAACAGTCAGAG AAATCAGAGTGGATGACGACCACGTGCAATCATGCACTGTACGCTATTTGTGACGTATTCACCCAGTTCT ACGAAGCTTTGCATGAAGTGCTTCTCTCTGATGTGTTTGCGCAGCTGCAGTGGTGCGTGAAACAAGATAA TGAGCAGTTGGCCCGATCAGGTACGAACTGCTTAGAGAACCTAGTGATATCCAACGGCGAGAAGTTCAGC CCTGCTGTCTGGGATGAAACCTGCAATTGCATGTTGGATATTTTCAAAACCACCATCCCACATGTTTTGC TGACGTGGAGACCTGCGGGGATGGAGGAGGAGGTGTCAGATAGACATCTGGATGTGGACCTAGACCGCCA GTCTTTAAGCAGCATAGACAGAAACGCCTCCGAGAGAGGACAGAGCCAGCTGTCCAACCCCACTGACGAC AGCTGGAAGGGGGCACCGTATGCACATCAGAAACTGCTGGCCAGCCTCCTCATCAAGTGTGTGGTTCAGC TGGAGCTGATCCAGACCATTGACAACATCGTGTTCTACCCTGCCACCAGCAAGAAGGAGGATGCGGAGCA CATGGTTGCTGCCCAGCAAGACACCCTCGATGCAGAGATCCACATCGAGACGGAGAATCAGGGCATGTAC AAGTTCATGTCCTCCCAGCACCTCTTCAAGCTGCTGGACTGCCTGCAGGAGTCCCACTCCTTCTCCAAAG CCTTCAACTCCAACTATGAGCAGCGGACTGTCCTCTGGCGAGCTGGCTTCAAGGGCAAGTCCAAACCCAA TCTTCTCAAACAAGAAACCAGCAGCCTGGCCTGTTGTCTAAGGATCCTGTTTAGAATGTACGTTGATGAG AACCGCAGGGATTCCTGGGACGAAATACAGCAGCGACTTCTAAGAGTGTGCAGTGAAGCACTTGCCTATT TCATTACTGTGAACTCCGAGAGCCATCGGGAAGCATGGACGAGCCTCCTGCTGCTACTTCTAACCAAAAC CCTCAAGATAAGCGATGAAAAGTTCAAAGCACACGCGTCAATGTACTACCCCTACCTGTGTGAAATTATG CAGTTTGACCTGATCCCTGAGCTCCGAGCAGTTCTGCGAAAGTTCTTCCTGCGGATAGGCCTGGTGTATA AGATATGGATTCCAGAAGAGCCGTCGCAAGTGCCAGCAGCACTGTCATCGACCTGG AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG213940 representing NM_001085495
Red=Cloning site Green=Tags(s) MQESQTKSMFVSRALEKILADKEVKRPQHSQLRRACQVALDEIKAELEKQRLGAAAPPKANFIEADKYFL PFELACQSKSPRVVSTSLDCLQKLIAYGHITGNAPDSGAPGKRLIDRIVETICNCFQGPQTDEGVQLQII KALLTAVTSPHIEIHEGTILQTVRTCYNIYLASKNLINQTTAKATLTQMLNVIFTRMENQVLQEARELEK PMQSKPQSPVIQATAGSPKFSRLKQSQAQSKPTTPEKAELPNGDHAQSGLGKVSLENGEAPRERGSPVSG RAEPSRGTDSGAQEVVKDILEDVVTSAVKEAAEKHGLPEPDRALGALECQECAVPPGVDENSQTNGIADD RQSLSSADNLEPDVQGHQVAARFSHILQKDAFLVFRSLCKLSMKPLGEGPPDPKSHELRSKVVSLQLLLS VLQNAGPVFRSHEMFVTAIKQYLCVALSKNGVSSVPDVFELSLAIFLTLLSNFKMHLKMQIEVFFKEIFL NILETSTSSFEHRWMVIQTLTRICADAQCVVDIYVNYDCDLNAANIFERLVNDLSKIAQGRSGHELGMTP LQELSLRKKGLECLVSILKCMVEWSKDLYVNPNHQATLGQERLPDQEMGDGKGLDMARRCSVTSVESTVS SGTQTAIQDDPEQFEVIKQQKEIIEHGIELFNKKPKRGIQFLQEQGMLGAAVEDIAQFLHQEERLDSTQV GEFLGDSTRFNKEVMYAYVDQLDFCEKEFVSALRTFLEGFRLPGEAQKIDRLMEKFAARYIECNQGQTLF ASADTAYVLAYSIIMLTTDLHSPQVKNKMTKEQYIKMNRGINDSKDLPEEYLSSIYDEIEGKKIAMKETK EHTIATKSTKQSVASEKQRRLLYNVEMEQMAKTAKALMEAVSHAKAPFTSATHLDHVRPMFKLVWTPLLA AYSIGLQNCDDTEVASLCLEGIRCAVRIACIFGMQLERDAYVQALARFSLLTASSSITEMKQKNIDTIKT LITVAHTDGNYLGNSWHEILKCISQLELAQLIGTGVKTRYLSGSGREREGSLKGHSLAGEEFMGLGLGNL VSGGVDKRQMASFQESVGETSSQSVVVAVDRIFTGSTRLDGNAIVDFVRWLCAVSMDELASPHHPRMFSL QKIVEISYYNMNRIRLQWSRIWHVIGDHFNKVGCNPNEDVAIFAVDSLRQLSMKFLEKGELANFRFQKDF LRPFEHIMKKNRSPTIRDMVIRCIAQMVSSQAANIRSGWKNIFAVFHQAASDHDGNIVELAFQTTGHIVS TIFQHHFPAAIDSFQDAVKCLSEFACNAAFPDTSMEAIRLIRFCGKYVSERPRVLQEYTSDDMNVAPGDR VWVRGWFPILFELSCIINRCKLDVRTRGLTVMFEIMKSYGHTFAKHWWQDLFRIVFRIFDNMKLPEQQSE KSEWMTTTCNHALYAICDVFTQFYEALHEVLLSDVFAQLQWCVKQDNEQLARSGTNCLENLVISNGEKFS PAVWDETCNCMLDIFKTTIPHVLLTWRPAGMEEEVSDRHLDVDLDRQSLSSIDRNASERGQSQLSNPTDD SWKGAPYAHQKLLASLLIKCVVQLELIQTIDNIVFYPATSKKEDAEHMVAAQQDTLDAEIHIETENQGMY KFMSSQHLFKLLDCLQESHSFSKAFNSNYEQRTVLWRAGFKGKSKPNLLKQETSSLACCLRILFRMYVDE NRRDSWDEIQQRLLRVCSEALAYFITVNSESHREAWTSLLLLLLTKTLKISDEKFKAHASMYYPYLCEIM QFDLIPELRAVLRKFFLRIGLVYKIWIPEEPSQVPAALSSTW SGPTRTRRLE - GFP Tag - V |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001085495 |
| ORF Size | 5376 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001085495.2, NP_001078964.1 |
| RefSeq Size | 8777 bp |
| RefSeq ORF | 5379 bp |
| Locus ID | 99371 |
| UniProt ID | A2A5R2 |
| Gene Summary | Promotes guanine-nucleotide exchange on ARF1 and ARF3 and to a lower extent on ARF5 and ARF6. Promotes the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP. Involved in the regulation of Golgi vesicular transport. Required for the integrity of the endosomal compartment. Involved in trafficking from the trans-Golgi network (TGN) to endosomes and is required for membrane association of the AP-1 complex and GGA1. Seems to be involved in recycling of the transferrin receptor from recycling endosomes to the plasma membrane. Probably is involved in the exit of GABA(A) receptors from the endoplasmic reticulum. Involved in constitutive release of tumor necrosis factor receptor 1 via exosome-like vesicles; the function seems to involve PKA and specifically PRKAR2B. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224934 | Arfgef2 (untagged) - Mouse ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (Arfgef2), (10ug) |
CNY 28,790.00 |
|
| MR213940 | Arfgef2 (Myc-DDK-tagged) - Mouse ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited) (Arfgef2) |
CNY 17,860.00 |
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