Nalcn (NM_177393) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A530023G15Rik; AI849508; Vgcnl1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224899 representing NM_177393 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCAAAAGAAAGCAGAGTTCCAGGGTGGAAGCCCAGCCAGTTACTGACTTTGGTCCTGATGAATCTC TGTCAGACAATGCTGACATACTCTGGATTAATAAGCCATGGGTGCACTCTCTGCTGCGCATCTGTGCCAT CATCAGCGTCATCTCAGTGTGCATGAACACACCTATGACCTTTGAGCACTATCCTCCTCTTCAGTATGTG ACCTTCACCTTGGACACTTTATTGATGTTCCTCTACACTGCAGAAATGATAGCAAAGATGCACATACGGG GAATTGTCAAGGGTGATAGCTCCTATGTGAAGGATCGCTGGTGTGTTTTTGATGGATTCATGGTCTTTTG CCTTTGGGTATCTCTTGTGTTACAGGTGTTTGAAATAGCTGACATAGTTGATCAGATGTCACCTTGGGGC ATGCTGCGGATCCCACGGCCACTCATTATGATCCGGGCTTTCAGGATTTATTTCCGATTCGAACTGCCAA GGACAAGAATTACAAACATTTTAAAGCGGTCAGGAGAACAAATATGGAGTGTTTCCATTTTCCTCCTTTT CTTCCTTCTTCTGTATGGGATTTTAGGAGTTCAAATGTTTGGAACATTTACCTACCACTGTGTAGTCAAT GACACAAAGCCAGGAAATGTAACCTGGAATAGCTTAGCTATTCCAGATACGCACTGCTCCCCAGAGCTAG AAGAAGGCTATCAATGCCCGCCAGGATTTAAATGCATGGACCTGGAAGACCTGGGACTTAGCAGGCAAGA GCTGGGCTACAGTGGCTTTAATGAGATAGGCACGAGTATATTCACAGTCTATGAGGCTTCATCTCAGGAA GGCTGGGTATTCCTCATGTACAGAGCAATCGACAGCTTTCCCCGCTGGCGCTCCTACTTCTACTTCATCA CACTGATTTTCTTCCTTGCTTGGCTTGTCAAGAATGTGTTTATTGCTGTCATCATTGAGACATTTGCAGA AATCAGAGTACAATTTCAACAAATGTGGGGAACTCGGAGCAGCACAACTTCTACAGCCACCACACAGATG TTCCATGAAGATGCCGCTGGTGGTTGGCAGCTGGTAGCTGTGGATGTCAACAAGCCCCAGGGACGTGCCC CAGCCTGCCTACAGAAAATGATGCGGTCATCGGTTTTCCACATGTTTATCCTGAGCATGGTGACTGTGGA TGTAATAGTTGCTGCCAGCAATTACTACAAGGGAGAGAACTTCAGAAGGCAGTATGATGAATTCTACCTT GCAGAGGTGGCTTTTACAGTCCTTTTCGACCTGGAAGCACTCCTGAAGATATGGTGTTTGGGGTTTACTG GCTACATCAGCTCATCTCTCCACAAGTTTGAACTATTGCTTGTTATTGGGACGACGCTTCATGTGTACCC AGATCTTTATCATTCTCAGTTCACATACTTCCAGGTTCTGCGGGTAGTCCGGCTTATTAAGATTTCCCCA GCATTGGAAGACTTTGTGTACAAGATATTTGGTCCTGGGAAAAAACTTGGAAGCTTGGTGGTGTTCACTG CCAGCCTCCTGATAGTTATGTCAGCCATCAGTTTGCAGATGTTCTGTTTTGTCGAAGAACTGGACAGATT CACCACTTTTCCAAGGGCATTTATGTCTATGTTCCAGATCCTCACCCAGGAAGGATGGGTGGATGTGATG GATCAGACTCTGAATGCTGTGGGGCATATGTGGGCACCACTGGTTGCCATCTATTTCATCCTCTATCATC TCTTTGCAACTTTGATCCTCCTGAGTTTGTTTGTTGCTGTTATTTTGGACAACTTAGAACTTGATGAAGA TCTAAAGAAGCTCAAACAACTAAAGCAAAGTGAAGCGAACGCGGACACCAAAGAAAAACTCCCTTTGCGC TTGAGAATCTTCGAAAAATTTCCAAACAGACCGCAAATGGTGAAAATCTCAAAACTTCCTTCAGATTTTA CAGTTCCTAAAATCAGGGAAAGCTTCATGAAGCAGTTCATTGACCGCCAGCAACAGGACACCTGCTGTCT CTTCAGAATCCTCCCCTCTACCTCTTCCTCATCATGTGACAACCCCAAGAAGCCCACAGCTGAAGACAAC AAATACATTGATCAAAAACTCCGCAAGTCTGTTTTCAGCATAAGGGCGAGGAACCTACTGGAAAAGGAGA CTGCAGTCACAAAAATCTTAAGAGCTTGCACTCGACAGCGCATGCTGAGCGGATCATTCGAGGGGCAGCC AGCAAAGGAGAGGTCGATCCTCAGCGTGCAGCATCACATCCGCCAGGAGCGCAGGTCACTAAGACATGGA TCCAACAGCCAGAGGATCAGCAGGGGGAAATCTCTTGAAACTTTAACTCAAGACCATTCAAATACAGTGC GCTACAGAAATGCACAAAGAGAAGACAGTGAAATAAAAATGATTCAGGAGAAGAAGGAACAAGCCGAGAT GAAAAGGAAGGTGCAAGAAGAGGAACTGCGGGAGAACCACCCATACTTTGACAAGCCTCTCTTCATCGTG GGGCGGGAGCACAGGTTCAGAAACTTCTGCCGCGTGGTGGTTCGAGCACGCTTCAATGCATCCAAAACAG ACCCTGTCACGGGAGCTGTGAAAAATACAAAGTACCACCAACTTTATGATTTGCTGGGACTTGTCACCTA CCTGGACTGGGTCATGATCACTGTAACCATCTGCTCTTGCATTTCTATGATGTTCGAATCCCCCTTCCGA AGAGTCATGCATGCACCCACTTTACAGATCGCAGAATATGTGTTTGTAATATTCATGAGCATTGAGCTTA ATCTGAAGATTATGGCAGATGGCTTGTTTTTCACTCCAACTGCTGTCATCAGGGACTTTGGTGGCGTCAT GGACATATTTATCTATCTTGTGAGTTTGATATTTCTTTGTTGGATGCCTCAAAATGTACCTGCCGAGTCA GGAGCCCAGCTACTGATGGTTCTTCGGTGCCTAAGACCTCTTCGGATATTCAAACTTGTGCCCCAAATGA GGAAAGTTGTTCGAGAACTTTTCAGTGGTTTCAAGGAAATATTTTTGGTCTCCATTCTGTTGTTGACGTT AATGCTTGTTTTTGCAAGCTTTGGGGTTCAGCTCTTTGCTGGAAAGTTAGCCAAGTGCAACGACCCCAAC ATTATCAGAAGGGAAGACTGTAATGGCATCTTCAGAATTAATGTAAGTGTGTCCAAGAACTTAAATTTAA AACTAAGACCTGGAGAGAAAAAACCTGGATTTTGGGTGCCCCGTGTTTGGGCAAATCCTCGGAACTTTAA CTTCGACAATGTGGGAAATGCCATGCTGGCATTATTTGAAGTTCTGTCCTTGAAAGGCTGGGTAGAAGTG AGGGATGTCATTATTCATCGTGTGGGGCCGATCCACGGAATCTATATTCATGTTTTCGTATTCCTGGGTT GCATGATCGGACTGACGCTTTTTGTCGGTGTAGTTATTGCTAACTTCAATGAAAACAAGGGGACAGCCCT GCTGACGGTAGATCAGAGACGATGGGAAGATCTCAAGAGCAGACTGAAGATCGCACAGCCTCTTCATCTC CCTCCTCGGCCGGATAATGATGGTTTTAGAGCTAAAATGTATGACATAACCCAGCATCCATTTTTTAAGA GGACAATTGCATTGCTGGTTCTGGCCCAGTCTGTGTTGCTATCTGTCAAGTGGGATGTTGACGATCCTGT GACGGTTCCTTTGGCAACAATGTCAGTTGTCTTCACCTTCATCTTTGTCTTAGAGGTTACAATGAAGATT ATAGCAATGTCACCAGCTGGATTCTGGCAAAGCAGAAGAAACCGATATGATCTCTTGGTGACATCTCTTG GTGTTGTGTGGGTGGTGCTCCATTTTGCTCTGCTGAATGCATACACCTACATGATGGGAGCCTGCGTGAT TGTCTTTAGATTTTTCTCCATCTGTGGGAAGCATGTGACATTAAAGATGCTCCTTCTGACTGTGGTTGTC AGCATGTACAAGAGCTTTTTTATCATCGTAGGAATGTTTCTCTTGCTGCTGTGCTATGCCTTTGCTGGAG TTGTTCTGTTTGGTACCGTAAAGTATGGTGAGAACATTAACAGGCATGCCAATTTTTCCTCAGCTGGCAA AGCCATTACTGTATTGTTTCGAATTGTCACAGGTGAAGACTGGAACAAGATTATGCATGATTGTATGGTT CAACCTCCATTTTGTACTCCAGATGAATTTACATACTGGGCAACAGACTGTGGCAATTATGCAGGGGCAC TTATGTACTTCTGCTCATTCTATGTCATCATTGCCTACATCATGCTGAATCTGCTTGTAGCCATAATTGT GGAGAATTTCTCTTTGTTTTATTCCACTGAAGAGGACCAGCTTTTGAGTTACAATGATCTTCGCCATTTT CAAATCATATGGAACATGGTAGATGATAAAAGAGAGGGTGTGATCCCCACTTTCCGAGTGAAGTTCCTGC TACGGCTGCTGCGTGGGAGGCTGGAAGTGGATCTTGATAAAGACAAGCTCCTGTTTAAGCATATGTGCTA TGAGATGGAGAGGCTGCACAATGGTGGTGATGTCACCTTCCATGATGTCTTAAGCATGCTCTCCTATCGC TCTGTAGACATCAGAAAAAGCCTGCAGCTAGAGGAGCTGCTGGCAAGGGAGCAGCTAGAATACACCATAG AGGAGGAGGTGGCTAAGCAGACTATTCGCATGTGGCTGAAGAAGTGCTTGAAACGCATCCGGGCTAAACA ACAGCAGTCGTGCAGCATCATCCACAGCCTGAGAGAGAGCCAGGAGCAAGAGCGGAGCCGGCTATTCCTG AATCCTCCCAGCATTGAGACCACCCAGCCAAGTGAGGACAGCAACGCCAACAGCCAGGACCACAGTATGC AACCTGAGACAAGCAGTCAGCAGCAGCTCTTAAGCCCTACTCTGTCAGACAGAGGAGGAAGCCGACAGGA TGCAGCAGATACTGGAAAACCCCAAAGGAAGATTGGGCAATGGCGTCTGCCCTCAGCCCCCAAACCAATA AGCCATTCTGTATCTTCGGTTAACCTACGGTTTGGAGGAAGGACAACGATGAAGACTGTGGTGTGCAAGA TGAACCCCATGCCAGACACAGCTTCCTGTGGCTCTGAAGTTAAAAAGTGGTGGACCAGACAGCTGACCGT GGAGAGTGACGAGAGTGGAGATGACCTCCTGGATATTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_177393
Insert Size	5220 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_177393.4, NP_796367.3
RefSeq Size	7115 bp
RefSeq ORF	5220 bp
Locus ID	338370
UniProt ID	Q8BXR5
Gene Summary	Voltage-independent, cation-nonselective channel which is permeable to sodium, potassium and calcium ions (PubMed:17448995). Regulates the resting membrane potential and controls neuronal excitability. Neuropeptides such as neurotensin and substance P (SP) stimulate the firing of action potentials by activating NALCN through a SRC family kinases-dependent pathway (PubMed:19092807). In addition to its baseline activity, NALCN activity is enhanced/modulated by several GPCRs (PubMed:19092807, PubMed:19575010, PubMed:21040849). Required for normal respiratory rhythm and neonatal survival. Involved in systemic osmoregulation by controlling the serum sodium concentration (PubMed:21177381). NALCN is partly responsible for the substance P-induced depolarization and regulation of the intestinal pace-making activity in the interstitial cells of Cajal (PubMed:22508057). Plays a critical role in both maintenance of spontaneous firing of substantia nigra pars reticulata (SNr) neurons and physiological modulation of SNr neuron excitability (PubMed:27177420).[UniProtKB/Swiss-Prot Function]

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