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Nalcn (NM_177393) Mouse Tagged ORF Clone
CAT＃: MG217430
- TrueORF®
Nalcn (tGFP-tagged) - Mouse sodium leak channel non-selective (Nalcn), (10ug)
ORF Plasmid: DDK
"NM_177393" in other vectors (2)
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CNY 19,100.00
货期*
9周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | A530023G15Rik; AI849508; Vgcnl1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG217430 representing NM_177393
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCAAAAGAAAGCAGAGTTCCAGGGTGGAAGCCCAGCCAGTTACTGACTTTGGTCCTGATGAATCTC TGTCAGACAATGCTGACATACTCTGGATTAATAAGCCATGGGTGCACTCTCTGCTGCGCATCTGTGCCAT CATCAGCGTCATCTCAGTGTGCATGAACACACCTATGACCTTTGAGCACTATCCTCCTCTTCAGTATGTG ACCTTCACCTTGGACACTTTATTGATGTTCCTCTACACTGCAGAAATGATAGCAAAGATGCACATACGGG GAATTGTCAAGGGTGATAGCTCCTATGTGAAGGATCGCTGGTGTGTTTTTGATGGATTCATGGTCTTTTG CCTTTGGGTATCTCTTGTGTTACAGGTGTTTGAAATAGCTGACATAGTTGATCAGATGTCACCTTGGGGC ATGCTGCGGATCCCACGGCCACTCATTATGATCCGGGCTTTCAGGATTTATTTCCGATTCGAACTGCCAA GGACAAGAATTACAAACATTTTAAAGCGGTCAGGAGAACAAATATGGAGTGTTTCCATTTTCCTCCTTTT CTTCCTTCTTCTGTATGGGATTTTAGGAGTTCAAATGTTTGGAACATTTACCTACCACTGTGTAGTCAAT GACACAAAGCCAGGAAATGTAACCTGGAATAGCTTAGCTATTCCAGATACGCACTGCTCCCCAGAGCTAG AAGAAGGCTATCAATGCCCGCCAGGATTTAAATGCATGGACCTGGAAGACCTGGGACTTAGCAGGCAAGA GCTGGGCTACAGTGGCTTTAATGAGATAGGCACGAGTATATTCACAGTCTATGAGGCTTCATCTCAGGAA GGCTGGGTATTCCTCATGTACAGAGCAATCGACAGCTTTCCCCGCTGGCGCTCCTACTTCTACTTCATCA CACTGATTTTCTTCCTTGCTTGGCTTGTCAAGAATGTGTTTATTGCTGTCATCATTGAGACATTTGCAGA AATCAGAGTACAATTTCAACAAATGTGGGGAACTCGGAGCAGCACAACTTCTACAGCCACCACACAGATG TTCCATGAAGATGCCGCTGGTGGTTGGCAGCTGGTAGCTGTGGATGTCAACAAGCCCCAGGGACGTGCCC CAGCCTGCCTACAGAAAATGATGCGGTCATCGGTTTTCCACATGTTTATCCTGAGCATGGTGACTGTGGA TGTAATAGTTGCTGCCAGCAATTACTACAAGGGAGAGAACTTCAGAAGGCAGTATGATGAATTCTACCTT GCAGAGGTGGCTTTTACAGTCCTTTTCGACCTGGAAGCACTCCTGAAGATATGGTGTTTGGGGTTTACTG GCTACATCAGCTCATCTCTCCACAAGTTTGAACTATTGCTTGTTATTGGGACGACGCTTCATGTGTACCC AGATCTTTATCATTCTCAGTTCACATACTTCCAGGTTCTGCGGGTAGTCCGGCTTATTAAGATTTCCCCA GCATTGGAAGACTTTGTGTACAAGATATTTGGTCCTGGGAAAAAACTTGGAAGCTTGGTGGTGTTCACTG CCAGCCTCCTGATAGTTATGTCAGCCATCAGTTTGCAGATGTTCTGTTTTGTCGAAGAACTGGACAGATT CACCACTTTTCCAAGGGCATTTATGTCTATGTTCCAGATCCTCACCCAGGAAGGATGGGTGGATGTGATG GATCAGACTCTGAATGCTGTGGGGCATATGTGGGCACCACTGGTTGCCATCTATTTCATCCTCTATCATC TCTTTGCAACTTTGATCCTCCTGAGTTTGTTTGTTGCTGTTATTTTGGACAACTTAGAACTTGATGAAGA TCTAAAGAAGCTCAAACAACTAAAGCAAAGTGAAGCGAACGCGGACACCAAAGAAAAACTCCCTTTGCGC TTGAGAATCTTCGAAAAATTTCCAAACAGACCGCAAATGGTGAAAATCTCAAAACTTCCTTCAGATTTTA CAGTTCCTAAAATCAGGGAAAGCTTCATGAAGCAGTTCATTGACCGCCAGCAACAGGACACCTGCTGTCT CTTCAGAATCCTCCCCTCTACCTCTTCCTCATCATGTGACAACCCCAAGAAGCCCACAGCTGAAGACAAC AAATACATTGATCAAAAACTCCGCAAGTCTGTTTTCAGCATAAGGGCGAGGAACCTACTGGAAAAGGAGA CTGCAGTCACAAAAATCTTAAGAGCTTGCACTCGACAGCGCATGCTGAGCGGATCATTCGAGGGGCAGCC AGCAAAGGAGAGGTCGATCCTCAGCGTGCAGCATCACATCCGCCAGGAGCGCAGGTCACTAAGACATGGA TCCAACAGCCAGAGGATCAGCAGGGGGAAATCTCTTGAAACTTTAACTCAAGACCATTCAAATACAGTGC GCTACAGAAATGCACAAAGAGAAGACAGTGAAATAAAAATGATTCAGGAGAAGAAGGAACAAGCCGAGAT GAAAAGGAAGGTGCAAGAAGAGGAACTGCGGGAGAACCACCCATACTTTGACAAGCCTCTCTTCATCGTG GGGCGGGAGCACAGGTTCAGAAACTTCTGCCGCGTGGTGGTTCGAGCACGCTTCAATGCATCCAAAACAG ACCCTGTCACGGGAGCTGTGAAAAATACAAAGTACCACCAACTTTATGATTTGCTGGGACTTGTCACCTA CCTGGACTGGGTCATGATCACTGTAACCATCTGCTCTTGCATTTCTATGATGTTCGAATCCCCCTTCCGA AGAGTCATGCATGCACCCACTTTACAGATCGCAGAATATGTGTTTGTAATATTCATGAGCATTGAGCTTA ATCTGAAGATTATGGCAGATGGCTTGTTTTTCACTCCAACTGCTGTCATCAGGGACTTTGGTGGCGTCAT GGACATATTTATCTATCTTGTGAGTTTGATATTTCTTTGTTGGATGCCTCAAAATGTACCTGCCGAGTCA GGAGCCCAGCTACTGATGGTTCTTCGGTGCCTAAGACCTCTTCGGATATTCAAACTTGTGCCCCAAATGA GGAAAGTTGTTCGAGAACTTTTCAGTGGTTTCAAGGAAATATTTTTGGTCTCCATTCTGTTGTTGACGTT AATGCTTGTTTTTGCAAGCTTTGGGGTTCAGCTCTTTGCTGGAAAGTTAGCCAAGTGCAACGACCCCAAC ATTATCAGAAGGGAAGACTGTAATGGCATCTTCAGAATTAATGTAAGTGTGTCCAAGAACTTAAATTTAA AACTAAGACCTGGAGAGAAAAAACCTGGATTTTGGGTGCCCCGTGTTTGGGCAAATCCTCGGAACTTTAA CTTCGACAATGTGGGAAATGCCATGCTGGCATTATTTGAAGTTCTGTCCTTGAAAGGCTGGGTAGAAGTG AGGGATGTCATTATTCATCGTGTGGGGCCGATCCACGGAATCTATATTCATGTTTTCGTATTCCTGGGTT GCATGATCGGACTGACGCTTTTTGTCGGTGTAGTTATTGCTAACTTCAATGAAAACAAGGGGACAGCCCT GCTGACGGTAGATCAGAGACGATGGGAAGATCTCAAGAGCAGACTGAAGATCGCACAGCCTCTTCATCTC CCTCCTCGGCCGGATAATGATGGTTTTAGAGCTAAAATGTATGACATAACCCAGCATCCATTTTTTAAGA GGACAATTGCATTGCTGGTTCTGGCCCAGTCTGTGTTGCTATCTGTCAAGTGGGATGTTGACGATCCTGT GACGGTTCCTTTGGCAACAATGTCAGTTGTCTTCACCTTCATCTTTGTCTTAGAGGTTACAATGAAGATT ATAGCAATGTCACCAGCTGGATTCTGGCAAAGCAGAAGAAACCGATATGATCTCTTGGTGACATCTCTTG GTGTTGTGTGGGTGGTGCTCCATTTTGCTCTGCTGAATGCATACACCTACATGATGGGAGCCTGCGTGAT TGTCTTTAGATTTTTCTCCATCTGTGGGAAGCATGTGACATTAAAGATGCTCCTTCTGACTGTGGTTGTC AGCATGTACAAGAGCTTTTTTATCATCGTAGGAATGTTTCTCTTGCTGCTGTGCTATGCCTTTGCTGGAG TTGTTCTGTTTGGTACCGTAAAGTATGGTGAGAACATTAACAGGCATGCCAATTTTTCCTCAGCTGGCAA AGCCATTACTGTATTGTTTCGAATTGTCACAGGTGAAGACTGGAACAAGATTATGCATGATTGTATGGTT CAACCTCCATTTTGTACTCCAGATGAATTTACATACTGGGCAACAGACTGTGGCAATTATGCAGGGGCAC TTATGTACTTCTGCTCATTCTATGTCATCATTGCCTACATCATGCTGAATCTGCTTGTAGCCATAATTGT GGAGAATTTCTCTTTGTTTTATTCCACTGAAGAGGACCAGCTTTTGAGTTACAATGATCTTCGCCATTTT CAAATCATATGGAACATGGTAGATGATAAAAGAGAGGGTGTGATCCCCACTTTCCGAGTGAAGTTCCTGC TACGGCTGCTGCGTGGGAGGCTGGAAGTGGATCTTGATAAAGACAAGCTCCTGTTTAAGCATATGTGCTA TGAGATGGAGAGGCTGCACAATGGTGGTGATGTCACCTTCCATGATGTCTTAAGCATGCTCTCCTATCGC TCTGTAGACATCAGAAAAAGCCTGCAGCTAGAGGAGCTGCTGGCAAGGGAGCAGCTAGAATACACCATAG AGGAGGAGGTGGCTAAGCAGACTATTCGCATGTGGCTGAAGAAGTGCTTGAAACGCATCCGGGCTAAACA ACAGCAGTCGTGCAGCATCATCCACAGCCTGAGAGAGAGCCAGGAGCAAGAGCGGAGCCGGCTATTCCTG AATCCTCCCAGCATTGAGACCACCCAGCCAAGTGAGGACAGCAACGCCAACAGCCAGGACCACAGTATGC AACCTGAGACAAGCAGTCAGCAGCAGCTCTTAAGCCCTACTCTGTCAGACAGAGGAGGAAGCCGACAGGA TGCAGCAGATACTGGAAAACCCCAAAGGAAGATTGGGCAATGGCGTCTGCCCTCAGCCCCCAAACCAATA AGCCATTCTGTATCTTCGGTTAACCTACGGTTTGGAGGAAGGACAACGATGAAGACTGTGGTGTGCAAGA TGAACCCCATGCCAGACACAGCTTCCTGTGGCTCTGAAGTTAAAAAGTGGTGGACCAGACAGCTGACCGT GGAGAGTGACGAGAGTGGAGATGACCTCCTGGATATT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG217430 representing NM_177393
Red=Cloning site Green=Tags(s) MLKRKQSSRVEAQPVTDFGPDESLSDNADILWINKPWVHSLLRICAIISVISVCMNTPMTFEHYPPLQYV TFTLDTLLMFLYTAEMIAKMHIRGIVKGDSSYVKDRWCVFDGFMVFCLWVSLVLQVFEIADIVDQMSPWG MLRIPRPLIMIRAFRIYFRFELPRTRITNILKRSGEQIWSVSIFLLFFLLLYGILGVQMFGTFTYHCVVN DTKPGNVTWNSLAIPDTHCSPELEEGYQCPPGFKCMDLEDLGLSRQELGYSGFNEIGTSIFTVYEASSQE GWVFLMYRAIDSFPRWRSYFYFITLIFFLAWLVKNVFIAVIIETFAEIRVQFQQMWGTRSSTTSTATTQM FHEDAAGGWQLVAVDVNKPQGRAPACLQKMMRSSVFHMFILSMVTVDVIVAASNYYKGENFRRQYDEFYL AEVAFTVLFDLEALLKIWCLGFTGYISSSLHKFELLLVIGTTLHVYPDLYHSQFTYFQVLRVVRLIKISP ALEDFVYKIFGPGKKLGSLVVFTASLLIVMSAISLQMFCFVEELDRFTTFPRAFMSMFQILTQEGWVDVM DQTLNAVGHMWAPLVAIYFILYHLFATLILLSLFVAVILDNLELDEDLKKLKQLKQSEANADTKEKLPLR LRIFEKFPNRPQMVKISKLPSDFTVPKIRESFMKQFIDRQQQDTCCLFRILPSTSSSSCDNPKKPTAEDN KYIDQKLRKSVFSIRARNLLEKETAVTKILRACTRQRMLSGSFEGQPAKERSILSVQHHIRQERRSLRHG SNSQRISRGKSLETLTQDHSNTVRYRNAQREDSEIKMIQEKKEQAEMKRKVQEEELRENHPYFDKPLFIV GREHRFRNFCRVVVRARFNASKTDPVTGAVKNTKYHQLYDLLGLVTYLDWVMITVTICSCISMMFESPFR RVMHAPTLQIAEYVFVIFMSIELNLKIMADGLFFTPTAVIRDFGGVMDIFIYLVSLIFLCWMPQNVPAES GAQLLMVLRCLRPLRIFKLVPQMRKVVRELFSGFKEIFLVSILLLTLMLVFASFGVQLFAGKLAKCNDPN IIRREDCNGIFRINVSVSKNLNLKLRPGEKKPGFWVPRVWANPRNFNFDNVGNAMLALFEVLSLKGWVEV RDVIIHRVGPIHGIYIHVFVFLGCMIGLTLFVGVVIANFNENKGTALLTVDQRRWEDLKSRLKIAQPLHL PPRPDNDGFRAKMYDITQHPFFKRTIALLVLAQSVLLSVKWDVDDPVTVPLATMSVVFTFIFVLEVTMKI IAMSPAGFWQSRRNRYDLLVTSLGVVWVVLHFALLNAYTYMMGACVIVFRFFSICGKHVTLKMLLLTVVV SMYKSFFIIVGMFLLLLCYAFAGVVLFGTVKYGENINRHANFSSAGKAITVLFRIVTGEDWNKIMHDCMV QPPFCTPDEFTYWATDCGNYAGALMYFCSFYVIIAYIMLNLLVAIIVENFSLFYSTEEDQLLSYNDLRHF QIIWNMVDDKREGVIPTFRVKFLLRLLRGRLEVDLDKDKLLFKHMCYEMERLHNGGDVTFHDVLSMLSYR SVDIRKSLQLEELLAREQLEYTIEEEVAKQTIRMWLKKCLKRIRAKQQQSCSIIHSLRESQEQERSRLFL NPPSIETTQPSEDSNANSQDHSMQPETSSQQQLLSPTLSDRGGSRQDAADTGKPQRKIGQWRLPSAPKPI SHSVSSVNLRFGGRTTMKTVVCKMNPMPDTASCGSEVKKWWTRQLTVESDESGDDLLDI TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_177393 |
| ORF Size | 5217 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_177393.4, NP_796367.3 |
| RefSeq Size | 7115 bp |
| RefSeq ORF | 5220 bp |
| Locus ID | 338370 |
| UniProt ID | Q8BXR5 |
| Gene Summary | Voltage-independent, cation-nonselective channel which is permeable to sodium, potassium and calcium ions (PubMed:17448995). Regulates the resting membrane potential and controls neuronal excitability. Neuropeptides such as neurotensin and substance P (SP) stimulate the firing of action potentials by activating NALCN through a SRC family kinases-dependent pathway (PubMed:19092807). In addition to its baseline activity, NALCN activity is enhanced/modulated by several GPCRs (PubMed:19092807, PubMed:19575010, PubMed:21040849). Required for normal respiratory rhythm and neonatal survival. Involved in systemic osmoregulation by controlling the serum sodium concentration (PubMed:21177381). NALCN is partly responsible for the substance P-induced depolarization and regulation of the intestinal pace-making activity in the interstitial cells of Cajal (PubMed:22508057). Plays a critical role in both maintenance of spontaneous firing of substantia nigra pars reticulata (SNr) neurons and physiological modulation of SNr neuron excitability (PubMed:27177420).[UniProtKB/Swiss-Prot Function] |
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