Chd1 (NM_007690) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4930525N21Rik; AI851787; AW555109
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224854 representing NM_007690 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGGACACAGTGATGAAGAAAGTGTTAGAAATGGCAGCGGAGAATCAAGTCAGTCAGGTGATGATT GTGGGTCAGCATCAGGCTCTGGATCTGGCTCGAGTTCTGGCAGCAGCAGTGACGGAAGCAGCAGCCAATC CGGGAGCAGCGACTCTGATTCTGGCTCTGACTCAGGAAGTCAATCAGAGTCTGAATCAGACACATCCCGA GAGAACAAGGTTCAAGCAAAACCACCAAAAGTCGACGGAGCCGAGTTTTGGAAATCTAGCCCCAGTATTC TGGCTGTCCAGAGATCTGCAATGCTTAGGAAGCAGCCACAGCAGGCCCAGCAGCAGCGCCCAGCTTCATC TAATAGTGGATCCGAAGAAGACTCGTCCAGCAGTGAAGACTCCGACGACTCGTCCAGCGGTGCCAAGAGG AAGAAGCACAATGATGAAGACTGGCAGATGTCTGGGTCCGGATCTCCATCTCAGTCTGGTTCAGACTCAG AATCTGAAGAAGAGCGAGATAAAAGCAGCTGCGACGGGACAGAGTCCGACTACGAGCCGAAAAACAAAGT CAGAAGCCGAAAGCCTCAGAATAGATCTAAGTCAAAAAATGGGAAAAAAATTCTTGGACAAAAAAAGAGA CAGATTGATTCATCTGAGGATGAAGATGATGAAGATTATGATAATGATAAACGAAGCTCTCGCCGCCAAG CCACCGTCAATGTGAGCTACAAGGAGGATGAAGAAATGAAAACCGACTCCGATGACCTGCTGGAGGTCTG CGGCGAGGACGTCCCTCAGCCTGAGGACGAGGAGTTTGAGACAATAGAGAGGGTTATGGATTGCAGAGTG GGGCGGAAAGGAGCTACTGGTGCTACTACAACCATTTATGCTGTCGAAGCAGATGGTGACCCAAATGCAG GATTTGAAAGAAACAAAGAGCCAGGAGACATACAGTATTTAATTAAGTGGAAAGGATGGTCTCACATCCA CAACACATGGGAGACAGAAGAGACCCTGAAGCAGCAGAACGTTAGAGGGATGAAAAAATTGGATAATTAT AAGAAAAAAGATCAAGAGACGAAACGATGGCTGAAAAATGCTTCTCCAGAAGATGTGGAATATTATAATT GCCAGCAAGAGCTTACAGATGATCTACACAAACAGTATCAGATAGTGGAGCGCATAATTGCTCATTCCAA TCAAAAATCAGCAGCTGGTCTTCCTGATTATTATTGCAAATGGCAGGGGCTTCCATACTCAGAGTGCAGC TGGGAGGATGGAGCTCTCATTTCCAAAAAGTTTCAGACATGCATCGATGAATATTTTAGCAGGAATCAGT CAAAAACGACACCTTTTAAAGATTGCAAAGTATTGAAACAAAGACCAAGATTTGTAGCTCTGAAGAAACA ACCATCCTATATTGGAGGACATGAGGGCTTAGAACTGAGAGACTATCAGCTGAATGGTTTAAACTGGCTC GCTCACTCTTGGTGCAAAGGAAATAGTTGCATACTTGCTGATGAAATGGGCCTTGGGAAAACAATACAGA CGATCTCATTTTTGAACTATTTGTTCCATGAACATCAGTTATATGGGCCGTTTCTACTAGTTGTCCCGCT CTCCACTCTGACTTCCTGGCAGAGGGAGATACAGACGTGGGCGTCTCAGATGAATGCTGTGGTTTACTTA GGCGACATTAACAGCAGAAACATGATAAGAACTCATGAATGGATGCATCCCCAGACCAAACGGTTAAAAT TTAATATACTTTTAACAACGTATGAAATTTTATTGAAGGATAAGGCATTCCTTGGTGGTCTGAATTGGGC ATTCATAGGTGTTGATGAAGCGCATCGATTAAAGAATGATGATTCCCTTCTGTACAAAACTTTAATCGAC TTTAAATCTAACCATCGCCTTCTGATCACTGGAACCCCTCTACAGAACTCCCTGAAGGAGCTCTGGTCAC TGCTGCACTTCATTATGCCGGAGAAGTTTTCTTCATGGGAAGATTTTGAAGAAGAACATGGCAAAGGCAG AGAATATGGTTATGCAAGCCTCCACAAGGAGCTTGAGCCATTTCTGTTACGCAGAGTTAAGAAAGATGTG GAAAAATCTCTTCCTGCCAAGGTGGAGCAGATTTTAAGAATGGAGATGAGTGCTTTACAGAAGCAATATT ACAAGTGGATTTTAACTAGGAATTATAAAGCCCTCAGCAAAGGTTCCAAGGGCAGTACCTCAGGCTTTTT GAACATTATGATGGAGCTAAAGAAATGTTGTAACCATTGCTACCTCATTAAACCACCAGATAATAATGAA TTCTATAATAAACAGGAGGCCTTACAACACTTAATCCGTAGTAGCGGAAAACTCATCCTTCTCGACAAGC TGTTGATTCGCCTAAGAGAAAGAGGCAACCGAGTGCTCATTTTCTCTCAGATGGTGCGGATGTTAGACAT ACTCGCAGAGTATTTGAAGTACCGCCAGTTCCCCTTTCAAAGATTAGATGGATCGATAAAAGGGGAGCTG AGGAAGCAGGCTTTGGATCACTTTAATGCCGAGGGCTCAGAGGATTTCTGCTTTTTGCTTTCTACCCGAG CTGGGGGTTTAGGCATTAACCTAGCCTCTGCTGACACCGTTGTTATATTCGATTCTGATTGGAATCCACA AAATGATCTTCAGGCTCAGGCAAGAGCTCACCGAATTGGACAGAAGAAACAGGTGAATATATATCGCTTG GTTACAAAGGGATCAGTTGAAGAAGATATTCTTGAAAGGGCCAAAAAGAAAATGGTTTTGGATCATCTTG TTATTCAAAGAATGGATACCACTGGGAAGACAGTGCTGCACACAGGCTCGGCTCCGTCAAGTTCCACCCC CTTCAATAAAGAGGAGTTATCCGCCATTTTAAAATTCGGTGCTGAGGAGCTCTTTAAGGAGCCTGAAGGA GAGGAGCAGGAGCCACAGGAAATGGATATCGATGAAATCCTGAAGAGAGCCGAGACTCACGAGAACGAGC CAGGCCCACTGAGTGTGGGGGATGAGCTGCTTTCTCAGTTTAAGGTTGCCAATTTTTCAAATATGGATGA AGATGACATTGAATTGGAACCTGAAAGAAATTCAAAGAACTGGGAGGAGATCATTCCAGAAGAGCAAAGA CGGCGACTAGAAGAGGAGGAGAGACAAAAGGAACTGGAGGAAATTTATATGCTCCCGAGAATGAGAAACT GTGCAAAGCAGATAAGTTTCAATGGAAGTGAAGGGAGGCGGAGTAGAAGCAGGAGATATTCTGGATCTGA TAGTGATTCAATCTCGGAAAGGAAACGGCCGAAGAAACGTGGGCGACCCCGCACTATCCCTCGGGAGAAT ATTAAAGGATTTAGTGATGCGGAGATTCGGCGGTTTATCAAGAGCTATAAGAAATTTGGTGGCCCCCTGG AGAGGTTAGATGCAATTGCTCGAGATGCTGAATTGGTTGATAAGTCAGAAACAGATCTCAGAAGACTAGG AGAACTGGTGCATAATGGTTGTGTTAAAGCTTTAAAAGACAGTTCTTCAGGAACAGAGCGAGCAGGTGGC AGACTTGGAAAAGTGAAGGGGCCAACATTCCGCATCTCTGGAGTCCAAGTGAATGCCAAGCTGGTCATTG CCCATGAGGATGAGCTGATCCCTCTGCATAAGTCCATCCCTTCGGACCCGGAGGAGAGGAAGCAGTATAC AATCCCCTGCCATACAAAGGCTGCGCACTTTGATATAGACTGGGGCAAAGAAGATGATTCTAATTTGTTA ATTGGTATCTATGAGTATGGCTATGGAAGCTGGGAAATGATTAAAATGGATCCAGACCTCAGTTTAACAC ACAAGATTCTTCCAGATGATCCTGATAAAAAACCACAAGCAAAACAGTTACAGACCCGTGCAGACTACCT CATCAAACTACTTAGCAGAGATCTTGCAAAAAGAGAGGCTCAGAGACTTTGTGGTGCGGGAGGTTCAAAG AGGAGAAAAACGAGAGCTAAAAAGAGTAAGGCAATGAAGTCCATCAAAGTGAAAGAGGAAATAAAGAGTG ACTCGTCGCCCCTGCCTTCAGAGAAGTCTGACGAGGATGACGATAAACTGAATGACTCCAAGCCTGAAAG TAAAGACCGATCCAAAAAGTCTGTAGTGTCCGATGCTCCCGTTCACATCACTGCGAGTGGAGAGCCCGTT CCCATAGCTGAAGAGTCTGAAGAGCTGGATCAGAAGACATTCAGTATTTGTAAAGAAAGAATGAGACCGG TGAAAGCAGCTTTGAAACAACTTGACAGGCCTGAGAAAGGCCTTTCAGAAAGAGAGCAGCTGGAACACAC TAGACAGTGCTTAATCAAGATCGGAGACCATATCACTGAATGCTTGAAGGAATATTCCAATCCTGAACAA ATTAAGCAGTGGAGGAAAAACCTGTGGATTTTTGTATCTAAGTTTACTGAGTTTGATGCAAGGAAATTAC ATAAATTATATAAGCATGCTATTAAAAAACGACAAGAATCTCAGCAAAACAGTGACCAGAATAGCAATGT TGCTACCACTCATGTGATTAGGAATCCAGATATGGAAAGGTTAAAAGAGAATACAAATCATGATGACAGT AGCAGGGACAGCTATTCTTCTGACAGACACTTATCTCAGTACCATGATCATCACAAGGACCGCCATCAGG GAGATTCTTATAAAAAGAGTGACTCTCGGAAGAGACCCTACTCCTCATTTAGCAATGGCAAAGACCACCG CGAGTGGGATCACTACAGGCAAGACAGCAGGTACTATAGTGACCGAGAGAAACACAGAAAACTGGATGAC CACAGGAGTCGAGAGCACAGGCCAAGTTTGGAAGGAGGCTTAAAGGACAGGTGTCACTCTGACCACCGAT CTCACTCGGACCATCGAATGCACTCAGACCATCGCTCAAGCTCCGAGCACACACATCATAAATCCTCCAG GGATTATCGGTATCTCTCAGATTGGCAGTTGGACCACCGAGCTGCCAGCAGTGGCCCTAGGTCACCTTTA GATCAGAGGTCTCCATATGGCTCCAGGTCCCCATTTGAACATTCAGCTGAACACAGAAGTACGCCTGAAC ACACCTGGAGTAGTCGGAAGACATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_007690
Insert Size	5136 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_007690.3, NP_031716.2
RefSeq Size	7918 bp
RefSeq ORF	5136 bp
Locus ID	12648
UniProt ID	P40201
Gene Summary	ATP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. Regulates polymerase II transcription. Also required for efficient transcription by RNA polymerase I, and more specifically the polymerase I transcription termination step. Regulates negatively DNA replication. Not only involved in transcription-related chromatin-remodeling, but also required to maintain a specific chromatin configuration across the genome. Required for the bridging of SNF2, the FACT complex, the PAF complex as well as the U2 snRNP complex to H3K4me3. Functions to modulate the efficiency of pre-mRNA splicing in part through physical bridging of spliceosomal components to H3K4me3 (By similarity). Required for maintaining open chromatin and pluripotency in embryonic stem cells (PubMed:19587682). Is also associated with histone deacetylase (HDAC) activity (PubMed:12890497).[UniProtKB/Swiss-Prot Function]

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