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Chd1 (NM_007690) Mouse Tagged ORF Clone
CAT＃: MG226492
- TrueORF®
Chd1 (tGFP-tagged) - Mouse chromodomain helicase DNA binding protein 1 (Chd1), (10ug)
ORF Plasmid: DDK
"NM_007690" in other vectors (2)
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CNY 20,710.00
货期*
3周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4930525N21Rik; AI851787; AW555109 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG226492 representing NM_007690
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGGACACAGTGATGAAGAAAGTGTTAGAAATGGCAGCGGAGAATCAAGTCAGTCAGGTGATGATT GTGGGTCAGCATCAGGCTCTGGATCTGGCTCGAGTTCTGGCAGCAGCAGTGACGGAAGCAGCAGCCAATC CGGGAGCAGCGACTCTGATTCTGGCTCTGACTCAGGAAGTCAATCAGAGTCTGAATCAGACACATCCCGA GAGAACAAGGTTCAAGCAAAACCACCAAAAGTCGACGGAGCCGAGTTTTGGAAATCTAGCCCCAGTATTC TGGCTGTCCAGAGATCTGCAATGCTTAGGAAGCAGCCACAGCAGGCCCAGCAGCAGCGCCCAGCTTCATC TAATAGTGGATCCGAAGAAGACTCGTCCAGCAGTGAAGACTCCGACGACTCGTCCAGCGGTGCCAAGAGG AAGAAGCACAATGATGAAGACTGGCAGATGTCTGGGTCCGGATCTCCATCTCAGTCTGGTTCAGACTCAG AATCTGAAGAAGAGCGAGATAAAAGCAGCTGCGACGGGACAGAGTCCGACTACGAGCCGAAAAACAAAGT CAGAAGCCGAAAGCCTCAGAATAGATCTAAGTCAAAAAATGGGAAAAAAATTCTTGGACAAAAAAAGAGA CAGATTGATTCATCTGAGGATGAAGATGATGAAGATTATGATAATGATAAACGAAGCTCTCGCCGCCAAG CCACCGTCAATGTGAGCTACAAGGAGGATGAAGAAATGAAAACTGACTCCGATGACCTGCTGGAGGTCTG CGGCGAGGACGTCCCTCAGCCTGAGGACGAGGAGTTTGAGACAATAGAGAGGGTTATGGATTGCAGAGTG GGGCGGAAAGGAGCTACTGGTGCTACTACAACCATTTATGCTGTCGAAGCAGATGGTGACCCAAATGCAG GATTTGAAAGAAACAAAGAGCCAGGAGACATACAGTATTTAATTAAGTGGAAAGGATGGTCTCACATCCA CAACACATGGGAGACAGAAGAGACCCTGAAGCAGCAGAACGTTAGAGGGATGAAAAAATTGGATAATTAT AAGAAAAAAGATCAAGAGACGAAACGATGGCTGAAAAATGCTTCTCCAGAAGATGTGGAATATTATAATT GCCAGCAAGAGCTTACAGATGATCTACACAAACAGTATCAGATAGTGGAGCGCATAATTGCTCATTCCAA TCAAAAATCAGCAGCTGGTCTTCCTGATTATTATTGCAAATGGCAGGGGCTTCCATACTCAGAGTGCAGC TGGGAGGATGGAGCTCTCATTTCCAAAAAGTTTCAGACATGCATCGATGAATATTTTAGCAGGAATCAGT CAAAAACGACACCTTTTAAAGATTGCAAAGTGTTGAAACAAAGACCAAGATTTGTAGCTCTGAAGAAACA ACCATCCTATATTGGAGGACATGAGGGCTTAGAACTGAGAGACTATCAGCTGAATGGTTTAAACTGGCTC GCTCACTCTTGGTGCAAAGGAAATAGTTGCATACTTGCTGATGAAATGGGCCTTGGGAAAACAATACAGA CGATCTCATTTTTGAACTATTTGTTCCATGAACATCAGTTATATGGGCCTTTTCTACTAGTTGTCCCGCT CTCCACACTGACTTCCTGGCAGAGGGAGATTCAGACGTGGGCGTCTCAGATGAATGCTGTGGTTTACTTA GGCGACATTAACAGCAGAAACATGATAAGAACTCATGAATGGATGCATCCCCAGACCAAACGGTTAAAAT TTAATATACTTTTAACAACGTATGAAATTTTATTGAAGGATAAGGCATTCCTTGGTGGTCTGAATTGGGC ATTCATAGGTGTTGATGAAGCGCATCGATTAAAGAATGATGATTCCCTTCTGTACAAAACTTTAATCGAC TTTAAATCTAACCATCGCCTTCTGATCACTGGAACCCCTCTACAGAACTCCCTGAAGGAGCTCTGGTCAC TGCTGCACTTCATTATGCCGGAGAAGTTTTCTTCATGGGAAGATTTTGAAGAAGAACATGGCAAAGGCAG AGAATATGGTTATGCAAGCCTCCACAAGGAGCTTGAGCCATTTCTGTTACGCAGAGTTAAGAAAGATGTG GAAAAATCTCTTCCTGCCAAGGTGGAGCAGATTTTAAGAATGGAGATGAGTGCTTTACAGAAGCAATATT ACAAGTGGATTTTAACTAGGAATTACAAAGCCCTCAGCAAAGGTTCCAAGGGCAGTACCTCAGGCTTTTT GAACATTATGATGGAGCTAAAGAAATGTTGTAACCATTGCTACCTCATTAAACCACCAGATAATAATGAA TTCTATAATAAACAGGAGGCCTTACAACACTTAATCCGTAGTAGCGGAAAACTCATCCTTCTCGACAAGC TGTTGATTCGCCTAAGAGAAAGAGGCAACCGAGTGCTCATTTTCTCTCAGATGGTGCGGATGTTAGACAT ACTCGCAGAGTATTTGAAGTACCGCCAGTTCCCCTTTCAAAGATTAGATGGATCGATAAAAGGGGAGCTG AGGAAGCAGGCTTTGGATCACTTTAATGCCGAGGGCTCAGAGGATTTCTGCTTTTTGCTTTCTACCCGAG CTGGGGGTTTAGGCATTAACCTAGCCTCTGCTGACACCGTTGTTATATTCGATTCTGATTGGAATCCACA AAATGATCTTCAGGCTCAGGCAAGAGCTCACCGAATTGGACAGAAGAAACAGGTGAATATATATCGCTTG GTTACAAAGGGATCAGTTGAAGAAGATATTCTTGAAAGGGCCAAAAAGAAAATGGTTTTGGATCATCTTG TTATTCAAAGAATGGATACCACTGGGAAGACAGTGCTGCACACAGGCTCGGCTCCGTCAAGTTCCACCCC CTTCAATAAAGAGGAGTTATCCGCCATTTTAAAATTCGGTGCTGAGGAGCTCTTTAAGGAGCCTGAAGGA GAGGAGCAGGAGCCACAGGAAATGGATATCGATGAAATCCTGAAGAGAGCCGAGACTCACGAGAACGAGC CAGGCCCACTGAGTGTGGGGGATGAGCTGCTTTCTCAGTTTAAGGTTGCCAATTTTTCAAATATGGATGA AGATGACATTGAATTGGAACCTGAAAGAAATTCAAAGAACTGGGAGGAGATCATTCCAGAAGAGCAAAGA CGGCGACTAGAAGAGGAGGAGAGACAAAAGGAACTGGAGGAAATTTATATGCTCCCGAGAATGAGAAACT GTGCAAAGCAGATAAGTTTCAATGGAAGTGAAGGGAGGCGGAGTAGAAGCAGGAGATATTCTGGATCTGA TAGTGATTCAATCTCGGAAAGGAAACGGCCGAAGAAACGTGGGCGACCCCGCACTATCCCTCGGGAGAAT ATTAAAGGATTTAGTGATGCGGAGATTCGGCGGTTTATCAAGAGCTATAAGAAATTTGGTGGCCCCCTGG AGAGGTTAGATGCAATTGCTCGAGATGCTGAATTGGTTGATAAGTCAGAAACAGATCTCAGAAGACTAGG AGAACTGGTGCATAATGGTTGTGTTAAAGCTTTAAAAGACAGTTCTTCAGGAACAGAGCGAGCAGGTGGC AGACTTGGAAAAGTGAAGGGGCCAACATTCCGCATCTCTGGAGTCCAAGTGAATGCCAAGCTGGTCATTG CCCATGAGGATGAGCTGATCCCTCTGCATAAGTCCATCCCTTCGGACCCGGAGGAGAGGAAGCAGTATAC AATCCCCTGCCATACAAAGGCTGCGCACTTTGATATAGACTGGGGCAAAGAAGATGATTCTAATTTGTTA ATTGGTATCTATGAGTATGGCTATGGAAGCTGGGAAATGATTAAAATGGATCCAGACCTCAGTTTAACAC ACAAGATTCTTCCAGATGATCCTGATAAAAAACCACAAGCAAAACAGTTACAGACCCGTGCAGACTACCT CATCAAACTACTTAGCAGAGATCTTGCAAAAAGAGAGGCTCAGAGACTTTGTGGTGCGGGAGGTTCAAAG AGGAGAAAAACGAGAGCTAAAAAGAGTAAGGCAATGAAGTCCATCAAAGTGAAAGAGGAAATAAAGAGTG ACTCGTCGCCCCTGCCTTCAGAGAAGTCTGACGAGGATGACGATAAACTGAATGACTCCAAGCCTGAAAG TAAAGACCGATCCAAAAAGTCTGTAGTGTCCGATGCTCCCGTTCACATCACTGCGAGTGGAGAGCCCGTT CCCATAGCTGAAGAGTCTGAAGAGCTGGATCAGAAGACATTCAGTATTTGTAAAGAAAGAATGAGACCGG TGAAAGCAGCTTTGAAACAACTTGACAGGCCTGAGAAAGGCCTTTCAGAAAGAGAGCAGCTGGAACACAC TAGACAGTGCTTAATCAAGATCGGAGACCATATCACTGAATGCTTGAAGGAATATTCCAATCCTGAACAA ATTAAGCAGTGGAGGAAAAACCTGTGGATTTTTGTATCTAAGTTTACTGAGTTTGATGCAAGGAAATTAC ATAAATTATATAAGCATGCTATTAAAAAACGACAAGAATCTCAGCAAAACAGTGACCAGAATAGCAATGT TGCTACCACTCATGTGATTAGGAATCCAGATATGGAAAGGTTAAAAGAGAATACAAATCATGATGACAGT AGCAGGGACAGCTATTCTTCTGACAGACACTTATCTCAGTACCATGATCATCACAAGGACCGCCATCAGG GAGATTCTTATAAAAAGAGTGACTCTCGGAAGAGACCCTACTCCTCATTTAGCAATGGCAAAGACCACCG CGAGTGGGATCACTACAGGCAAGACAGCAGGTACTATAGTGACCGAGAGAAACACAGAAAACTGGATGAC CACAGGAGTCGAGAGCACAGGCCAAGTTTGGAAGGAGGCTTAAAGGACAGGTGTCACTCTGACCACCGAT CTCACTCGGACCATCGAATGCACTCAGACCATCGCTCAAGCTCCGAGCACACACATCATAAATCCTCCAG GGATTATCGGTATCTCTCAGATTGGCAGTTGGACCACCGAGCTGCCAGCAGTGGCCCTAGGTCACCTTTA GATCAGAGGTCTCCATATGGCTCCAGGTCCCCATTTGAACATTCAGCTGAACACAGAAGTACGCCTGAAC ACACCTGGAGTAGTCGGAAGACA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG226492 representing NM_007690
Red=Cloning site Green=Tags(s) MNGHSDEESVRNGSGESSQSGDDCGSASGSGSGSSSGSSSDGSSSQSGSSDSDSGSDSGSQSESESDTSR ENKVQAKPPKVDGAEFWKSSPSILAVQRSAMLRKQPQQAQQQRPASSNSGSEEDSSSSEDSDDSSSGAKR KKHNDEDWQMSGSGSPSQSGSDSESEEERDKSSCDGTESDYEPKNKVRSRKPQNRSKSKNGKKILGQKKR QIDSSEDEDDEDYDNDKRSSRRQATVNVSYKEDEEMKTDSDDLLEVCGEDVPQPEDEEFETIERVMDCRV GRKGATGATTTIYAVEADGDPNAGFERNKEPGDIQYLIKWKGWSHIHNTWETEETLKQQNVRGMKKLDNY KKKDQETKRWLKNASPEDVEYYNCQQELTDDLHKQYQIVERIIAHSNQKSAAGLPDYYCKWQGLPYSECS WEDGALISKKFQTCIDEYFSRNQSKTTPFKDCKVLKQRPRFVALKKQPSYIGGHEGLELRDYQLNGLNWL AHSWCKGNSCILADEMGLGKTIQTISFLNYLFHEHQLYGPFLLVVPLSTLTSWQREIQTWASQMNAVVYL GDINSRNMIRTHEWMHPQTKRLKFNILLTTYEILLKDKAFLGGLNWAFIGVDEAHRLKNDDSLLYKTLID FKSNHRLLITGTPLQNSLKELWSLLHFIMPEKFSSWEDFEEEHGKGREYGYASLHKELEPFLLRRVKKDV EKSLPAKVEQILRMEMSALQKQYYKWILTRNYKALSKGSKGSTSGFLNIMMELKKCCNHCYLIKPPDNNE FYNKQEALQHLIRSSGKLILLDKLLIRLRERGNRVLIFSQMVRMLDILAEYLKYRQFPFQRLDGSIKGEL RKQALDHFNAEGSEDFCFLLSTRAGGLGINLASADTVVIFDSDWNPQNDLQAQARAHRIGQKKQVNIYRL VTKGSVEEDILERAKKKMVLDHLVIQRMDTTGKTVLHTGSAPSSSTPFNKEELSAILKFGAEELFKEPEG EEQEPQEMDIDEILKRAETHENEPGPLSVGDELLSQFKVANFSNMDEDDIELEPERNSKNWEEIIPEEQR RRLEEEERQKELEEIYMLPRMRNCAKQISFNGSEGRRSRSRRYSGSDSDSISERKRPKKRGRPRTIPREN IKGFSDAEIRRFIKSYKKFGGPLERLDAIARDAELVDKSETDLRRLGELVHNGCVKALKDSSSGTERAGG RLGKVKGPTFRISGVQVNAKLVIAHEDELIPLHKSIPSDPEERKQYTIPCHTKAAHFDIDWGKEDDSNLL IGIYEYGYGSWEMIKMDPDLSLTHKILPDDPDKKPQAKQLQTRADYLIKLLSRDLAKREAQRLCGAGGSK RRKTRAKKSKAMKSIKVKEEIKSDSSPLPSEKSDEDDDKLNDSKPESKDRSKKSVVSDAPVHITASGEPV PIAEESEELDQKTFSICKERMRPVKAALKQLDRPEKGLSEREQLEHTRQCLIKIGDHITECLKEYSNPEQ IKQWRKNLWIFVSKFTEFDARKLHKLYKHAIKKRQESQQNSDQNSNVATTHVIRNPDMERLKENTNHDDS SRDSYSSDRHLSQYHDHHKDRHQGDSYKKSDSRKRPYSSFSNGKDHREWDHYRQDSRYYSDREKHRKLDD HRSREHRPSLEGGLKDRCHSDHRSHSDHRMHSDHRSSSEHTHHKSSRDYRYLSDWQLDHRAASSGPRSPL DQRSPYGSRSPFEHSAEHRSTPEHTWSSRKT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007690 |
| ORF Size | 5133 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007690.3, NP_031716.2 |
| RefSeq Size | 7918 bp |
| RefSeq ORF | 5136 bp |
| Locus ID | 12648 |
| UniProt ID | P40201 |
| Gene Summary | ATP-dependent chromatin-remodeling factor which functions as substrate recognition component of the transcription regulatory histone acetylation (HAT) complex SAGA. Regulates polymerase II transcription. Also required for efficient transcription by RNA polymerase I, and more specifically the polymerase I transcription termination step. Regulates negatively DNA replication. Not only involved in transcription-related chromatin-remodeling, but also required to maintain a specific chromatin configuration across the genome. Required for the bridging of SNF2, the FACT complex, the PAF complex as well as the U2 snRNP complex to H3K4me3. Functions to modulate the efficiency of pre-mRNA splicing in part through physical bridging of spliceosomal components to H3K4me3 (By similarity). Required for maintaining open chromatin and pluripotency in embryonic stem cells (PubMed:19587682). Is also associated with histone deacetylase (HDAC) activity (PubMed:12890497).[UniProtKB/Swiss-Prot Function] |
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