Gemin5 (NM_172558) Mouse Untagged Clone

Name: Gemin5 (NM_172558) Mouse Untagged Clone
SKU: MC224604
Price: 19190.00

CAT＃: MC224604

Gemin5 (untagged) - Mouse gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant 2, (10ug)

Datasheet

SDS

"NM_172558" in other vectors (4)

CNY 19,190.00

货期*

8周

规格

Product images

经常一起买 (3)

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

CNY 4,090.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AA407055; AA407208; AI451603; BB194447; C330013N08
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224604 representing NM_172558 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCCGGAGCCGCGGACGCTGCCGCCGTCCCCCAACTGGTACTGCTCACGCTGCAGCGACGCGGCGC CCGGAGGCATCTTTGGCTTCGCTGCGCGGACCTCCGTCTTCCTCGTCCGCGTGGGTCCGGGTGCGGGCGC GAGCCCAGGGGCGCCCCCGTTTCGAGTGGTAGGAGAGTTGGTGGGACACACTGAAAGAGTATCTGGTTTC ACTTTTTCTCATCACCCTGGACAATACAACCTCTGTGCCACCAGCTCAGACGATGGAACTGTGAAGGTTT GGGATGTAGAGACCAAGACTGTAGTGACAGAACACACACTCCATCAGCATACAATATCAGCACTGCATTG GTCTCCGACAGTTAAGGACTTAATAGTCTCTGGGGATGAAAAAGGAGTCGTTTTCTGTTACTGGCTTAAC AGAAATGACAGCCAGCACCTCTTCACAGAGCCCAGGACCATATTCTGTCTGACGTGTTCACCTCATCACG AAAATCTAGTAGCCATTGGCTACAAAGATGGCATAGTGGTTATCATTGACATCAGTAAAAAGGGAGAAGT TATTCACAGACTTCGAGGCCACGATGACGAGATCCACTCCATAGCCTGGTGTCCTCTGTCTGGTGAAGAC TGCTTATCCATCAGCCAAGAGGAAAACTCGGAAGAACCTGACATTCCCAATGGGAAGCTTATAGCAGAAA CCCCCATCACAAAAGGCTGCTACTTAGCCACAGGAAGCAAAGATCAGACCATTCGAATCTGGAGCTGTTC CCGCGGCCGGGGTGTAATGGTTTTGAAATTGCCCTTCCTGAAGAGAAGAAGTGGGGGTGTGGACCCAACA GTTAAAGAACGGCTTTGGTTGACACTTCATTGGCCTAAGAACCAACCAACACAGCTGGTATCCAGTTGTT TTGGAGGTGAGCTGCTGCTATGGGACCTCACCCAGTCATGGAGGCGGAAGTACACGCTCTTCAGCACCTC AGCAGAAGGGCACAATCATTCCAGAATTGTCTTCAATCTGTGTTCTCTGAAGACTGAAGATGGCAAGCAG CTGCTACTGTCCACATCCATGGATAGAGATGTAAAATGTTGGGACATGGCCACCCTGGAGTGTTGCTGGA CATTGCCTTCTCTTGGAGGTTTTGCCTACAGCCTGGCCTTTTCTCCTGTGGACGTGGGCTCCCTGGCCAT AGGTGTTGGGGACGGCATGATCCGGGTGTGGAATACACTCTCCATAAAGAACAACTACGACGTGAAAAAC TTCTGGCAAGGAGTCAAGTCCAAGGTCACAGCGCTATGCTGGCACCCAAACAAGGAAGGCTGCTTAGCCT TCGGGACCGATGACGGAAAAGTGGGATTGTATGACACCTGCTCCAACAAACCTCCACAGATTTCAAGCAC GTATCATAAGAAGACCGTGTATAGGTTGGCTTGGGGGCCACCAGTCCCCCCCATGTCACTTGGAGGGGAA GGAGACAGGCCTTCCCTCACCTTGTACAGCTGTGGAGGGGAAGGCGTTGTGCTACAGCATAACCCCTGGA AGCTCAGTGGAGAGGCCTTCGACATCAACAAACTCGTGAGGGACACCAACTCCATTAGATACAAACTGCC CGTGCACACAGAGATCAGCTGGAAAGGAGATGGCAAAGTCCTGGCTCTTGGGAATGAAGATGGATCAATA GAAATATTCCAAGTCCCCAACTTGAGGCTGCTGTGCACCATCCAGCAGCACCACAAGCTTGTGAACGCCA TAGTCTGGCACCATGAGCATGGCAGCCGTCCAGAGCTGAGCTGTCTCCTTGCTTCTGGCTCCAACAATGC AGTCATCTACGTGCACAACCTGAAAGCAGTCCTAGAAAGCAATCCTGAGTCTCCAATAACCATCACAGAG CCCTACCGCACCCTCTCGGGGCACACAGCCAAGATTACCAGTCTGGCATGGAGCCCACATCATGACGGAA GGCTGGTATCTGCTTGCTACGATGGCACAGCTCAGGTGTGGGATGCCCTCCGGGAAGAACCTTTGTTCAA TTTCCGAGGACATCGAGGCCGGCTGCTATGTGTCGCGTGGTCCCCAGTGGATCCAGAATGCATCTACTCA GGTGCAGATGACTTCTGTGTCTACAGGTGGCTGACTTCCATGCAGGACCATTCCCGGCCTCCTCAAGGGA AAAAATGTATTGAACTAGAGAAAAAACGACTCTCTCAATTTAAGCCAAAGCTTAAAAAGAAGAAAAAACC TACCTTGCGACTGCCTGTAAAGCAGGATTCATCCGTTGGCAATGAAGATGAGAGTGTAAAGGAGAACTCT GGACCTGCTGAGAACGGCTTGTCGGACCAGGATGGTGAGGAGGAAGCCCAGGAGCCAGAGCTGCCTCCCT CTCCTGTAGTTTGTGTAGAACCTGTTTCCTGTACTGACATTTGCTCAGGCTTTGAAAAGTCAAAAGTCAC TGTTAGTAGCAAAGCCACCTCACTGAAAAAGGAGCCAGCTAAAGAGAAACCAGAAGCCTTGCTCAAGAAG AGGAAGGCTCGCTCCATGCTCCCTCTGAGCACCAGCTTAGACCACAGGTCCAAAGAGGAGCTCCATCGAG ACTGCTTGGTACTAGCAACTGCAACACACGCCAAAGCAGAGCTAAATGAAGATGTGTCTGCCGACCTTGA GGAACGATTCCACTTGGGCCTTTTCACAGACAGGGCTACTCTATACAGGATGATGGAAACAGAAGGGAAA GGTCACTTGGAAAGTGGCCACCCCGAGCTTTTTCACCAGCTCATGCTCTGGAAAGGAGATCTGAAGGGTG TTCTCCAGGCTGCAGCAGAGAGAGGCGAGCTGACAGACAGTCTCGTGGCTGTGGCACCAGTGGCTGGCTA CAGTGTATGGCTGTGGGCTGTGGAAGCCTTTGCCAAGCAGCTGTGTTTCCAGGACCAGTACGTCAAGGCT GCCTCTTATCTGCTATCCATCCACAAAGTGTACGAAGCTGTGGAACTTCTCAAGTCAAACCATCTCTACA GAGAAGCTATTGCAGTTGCCAAGGCTCGGCTGCGCCCTGAGGACCCTGTTCTGAAGGAACTATACCTCAG CTGGGGGTCCATCCTGGAGAGAGATGGCCACTATGCTATTGCAGCCAAGTGCTACTTAGGAGCCACTTCC GCCTATGATGCAGCCAAAGTTCTGGCCAGAAAGGGAGATGCAGCCTCACTTAGAACAGCTGCAGAGCTGG CTGCCATAGCAGGAGAACATGAGCTGGCTGCTTCCCTGGCTCTTAGATGTGCCCAAGAGCTGCTTCTGAT GAAGAACTGGGTGGGTGCACAAGAAGCATTGGGACTGCATGAAAGCCTGCAGGGCCAGAGACTGGTCTTC TGCCTCCTTGAACTTCTATGCCGGCACCTGGAGGAAAAACAGCCTCTAGAGGTCAGAGGCCCCTCTTCCA TTTACCACCAGTGGGCCACAGGTTCTGAGGGAACCTTGGTGCAGAGAGTAACCGGTGTGTGGCGCAGCGC CTTCAGTGTGGACACCCCGGAGCAGTGTCAGGCCGCCTTACAGAAGCTGCAGGATGTCAAGTACCCATCA GCAACAAGTAACACTCCCTTCAGACAGCTGCTGCTTCATGTCTGCCATGACCTGACCTTGGCAATGCTGA GCCAGCAGGCTGCTGCCTGGGAGGAGGCAGTGCCTGCTCTGCTGCAGGCTGTGGTTCGGAGCTATACCTC AGGGAACTTCACCCTCATGCAGGAAATCTACTCAGCCTTTCTCCCAGGTGGCTGTGACCACCTACGAGAC AAACTGGGTGACCTCTCTCCTGCCATGGCAGCTTACAAAAGCTTAGAGGCCTTTTGTATTTATGGGCAAC TGTATGAAGTCTGGTGGTCGCTCTGCGGGCCTGGCCCTGAGTCAAGCGTCTGGGTGTTGTCAGCTGAAAG CACGGTCTCTGATAAACAGAGCAAGCCAGAGGACAGTGCCAGTGCTGAGGACATGGAACAGCCTCCAGGC CCGGGCCCGAGGCTCAGTGCAGAGAGTGAGCGTTTGCTGAGTGCCTGCAAGGAGCTTTTCTCAGAACGGC ATGCCAGCCTGCAGACCTCCCAGAGGACTGTAGCCGAAGTCCAAGAGACACTGGCAGAAATGATCCGCCA GCACCAAAAGAGTCAGCTCTGCAAGGCCACAACAAATGGTCCCAGCAGAGATGAACCCAGCAGAGATGAA CCCAGCCAGGAAGCAGAGCGGGCTCCTTCTCAGCCTCCGAGCCCAACAGAAGAAAGAAATGCACCAGTGT CTCTCCCTGAACTAACCAGAAGGCTCACAGAGGCAAATGAGAGGATAGCAGAGTTTCCAGAGAGTGTAAA GGCCTGGCCCTTTCCAGATGTGCTGGAGTGCTGTCTCGTCTTGCTTCACATTGGGTCCCAGTGTCCTGAC GCTGTGGACCCTGAGATGCAGCAACAGGCCCAGGAGCTCCTTCACAAGTATGGGCACACTCGAGCTTACA GAAGACACTGCCAGAGTCGGCACACGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_172558
Insert Size	4509 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_172558.3, NP_766146.2
RefSeq Size	6226 bp
RefSeq ORF	4509 bp
Locus ID	216766
UniProt ID	Q8BX17
Gene Summary	Required for the assembly of the SMN complex that plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. GEMIN5 acts as the snRNA-binding protein of the SMN complex. Binds to the 7-methylguanosine cap of RNA molecules (By similarity). Binds to the 3' UTR of SMN1 mRNA and regulates its translation; does not affect mRNA stability (PubMed:25911097). May play a role in the regulation of protein synthesis via its interaction with ribosomes (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is 1 aa shorter compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
MG218608	Gemin5 (tGFP-tagged) - Mouse gem (nuclear organelle) associated protein 5 (Gemin5) transcript variant 2, (10ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 16,530.00
MR218608	Gemin5 (Myc-DDK-tagged) - Mouse gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant 2	10 ug 产品选项验证失败所有选项都是必需的	CNY 15,010.00
MR218608L3	Lenti ORF clone of Gemin5 (Myc-DDK-tagged) - Mouse gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant 2	10 ug 产品选项验证失败所有选项都是必需的	CNY 16,910.00
MR218608L4	Lenti ORF clone of Gemin5 (mGFP-tagged) - Mouse gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant 2	10 ug 产品选项验证失败所有选项都是必需的	CNY 16,910.00

其它Gemin5产品

Expression Plasmids

Lentiviral Particles

CRISPR

RNAi

Proteins

Antibodies