United States
Germany
Japan
United Kingdom
China
Gemin5 (NM_172558) Mouse Tagged ORF Clone
CAT＃: MR218608
- TrueORF®
Gemin5 (Myc-DDK-tagged) - Mouse gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant 2
ORF Plasmid: tGFP
"NM_172558" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 15,010.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AA407055; AA407208; AI451603; BB194447; C330013N08 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR218608 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCCGGAGCCGCGGACGCTGCCGCCGTCCCCCAACTGGTACTGCTCACGCTGCAGCGACGCGGCGC CCGGAGGCATCTTTGGCTTCGCTGCGCGGACCTCCGTCTTCCTCGTCCGCGTGGGTCCGGGTGCGGGCGC GAGCCCAGGGGCGCCCCCGTTTCGAGTGGTAGGAGAGTTGGTGGGACACACTGAAAGAGTATCTGGTTTC ACTTTTTCTCATCACCCTGGACAATACAACCTCTGTGCCACCAGCTCAGACGATGGAACTGTGAAGGTTT GGGATGTAGAGACCAAGACTGTAGTGACAGAACACACACTCCATCAGCATACAATATCAGCACTGCATTG GTCTCCGACAGTTAAGGACTTAATAGTCTCTGGGGATGAAAAAGGAGTCGTTTTCTGTTACTGGCTTAAC AGAAATGACAGCCAGCACCTCTTCACAGAGCCCAGGACCATATTCTGTCTGACGTGTTCACCTCATCACG AAAATCTAGTAGCCATTGGCTACAAAGATGGCATAGTGGTTATCATTGACATCAGTAAAAAGGGAGAAGT TATTCACAGACTTCGAGGCCACGATGACGAGATCCACTCCATAGCCTGGTGTCCTCTGTCTGGTGAAGAC TGCTTATCCATCAGCCAAGAGGAAAACTCGGAAGAACCTGACATTCCCAATGGGAAGCTTATAGCAGAAA CCCCCATCACAAAAGGCTGCTACTTAGCCACAGGAAGCAAAGATCAGACCATTCGAATCTGGAGCTGTTC CCGCGGCCGGGGTGTAATGGTTTTGAAATTGCCCTTCCTGAAGAGAAGAAGTGGGGGTGTGGACCCAACA GTTAAAGAACGGCTTTGGTTGACACTTCATTGGCCTAAGAACCAACCAACACAGCTGGTATCCAGTTGTT TTGGAGGTGAGCTGCTGCTATGGGACCTCACCCAGTCATGGAGGCGGAAGTACACGCTCTTCAGCACCTC AGCAGAAGGGCACAATCATTCCAGAATTGTCTTCAATCTGTGTTCTCTGAAGACTGAAGATGGCAAGCAG CTGCTACTGTCCACATCCATGGATAGAGATGTAAAATGTTGGGACATGGCCACCCTGGAGTGTTGCTGGA CATTGCCTTCTCTTGGAGGTTTTGCCTACAGCCTGGCCTTTTCTCCTGTGGACGTGGGCTCCCTGGCCAT AGGTGTTGGGGACGGCATGATCCGGGTGTGGAATACACTCTCCATAAAGAACAACTACGACGTGAAAAAC TTCTGGCAAGGAGTCAAGTCCAAGGTCACAGCGCTATGCTGGCACCCAAACAAGGAAGGCTGCTTAGCCT TCGGGACCGATGACGGAAAAGTGGGATTGTATGACACCTGCTCCAACAAACCTCCACAGATTTCAAGCAC GTATCATAAGAAGACCGTGTATAGGTTGGCTTGGGGGCCACCAGTCCCCCCCATGTCACTTGGAGGGGAA GGAGACAGGCCTTCCCTCACCTTGTACAGCTGTGGAGGGGAAGGCGTTGTGCTACAGCATAACCCCTGGA AGCTCAGTGGAGAGGCCTTCGACATCAACAAACTCGTGAGGGACACCAACTCCATTAGATACAAACTGCC CGTGCACACAGAGATCAGCTGGAAAGGAGATGGCAAAGTCCTGGCTCTTGGGAATGAAGATGGATCAATA GAAATATTCCAAGTCCCCAACTTGAGGCTGCTGTGCACCATCCAGCAGCACCACAAGCTTGTGAACGCCA TAGTCTGGCACCATGAGCATGGCAGCCGTCCAGAGCTGAGCTGTCTCCTTGCTTCTGGCTCCAACAATGC AGTCATCTACGTGCACAACCTGAAAGCAGTCCTAGAAAGCAATCCTGAGTCTCCAATAACCATCACAGAG CCCTACCGCACCCTCTCGGGGCACACAGCCAAGATTACCAGTCTGGCATGGAGCCCACATCATGACGGAA GGCTGGTATCTGCTTGCTACGATGGCACAGCTCAGGTGTGGGATGCCCTCCGGGAAGAACCTTTGTTCAA TTTCCGAGGACATCGAGGCCGGCTGCTATGTGTCGCGTGGTCCCCAGTGGATCCAGAATGCATCTACTCA GGTGCAGATGACTTCTGTGTCTACAGGTGGCTGACTTCCATGCAGGACCATTCCCGGCCTCCTCAAGGGA AAAAATGTATTGAACTAGAGAAAAAACGACTCTCTCAATTTAAGCCAAAGCTTAAAAAGAAGAAAAAACC TACCTTGCGACTGCCTGTAAAGCAGGATTCATCCGTTGGCAATGAAGATGAGAGTGTAAAGGAGAACTCT GGACCTGCTGAGAACGGCTTGTCGGACCAGGATGGTGAGGAGGAAGCCCAGGAGCCAGAGCTGCCTCCCT CTCCTGTAGTTTGTGTAGAACCTGTTTCCTGTACTGACATTTGCTCAGGCTTTGAAAAGTCAAAAGTCAC TGTTAGTAGCAAAGCCACCTCACTGAAAAAGGAGCCAGCTAAAGAGAAACCAGAAGCCTTGCTCAAGAAG AGGAAGGCTCGCTCCATGCTCCCTCTGAGCACCAGCTTAGACCACAGGTCCAAAGAGGAGCTCCATCGAG ACTGCTTGGTACTAGCAACTGCAACACACGCCAAAGCAGAGCTAAATGAAGATGTGTCTGCCGACCTTGA GGAACGATTCCACTTGGGCCTTTTCACAGACAGGGCTACTCTATACAGGATGATGGAAACAGAAGGGAAA GGTCACTTGGAAAGTGGCCACCCCGAGCTTTTTCACCAGCTCATGCTCTGGAAAGGAGATCTGAAGGGTG TTCTCCAGGCTGCAGCAGAGAGAGGCGAGCTGACAGACAGTCTCGTGGCTGTGGCACCAGTGGCTGGCTA CAGTGTATGGCTGTGGGCTGTGGAAGCCTTTGCCAAGCAGCTGTGTTTCCAGGACCAGTACGTCAAGGCT GCCTCTTATCTGCTATCCATCCACAAAGTGTACGAAGCTGTGGAACTTCTCAAGTCAAACCATCTCTACA GAGAAGCTATTGCAGTTGCCAAGGCTCGGCTGCGCCCTGAGGACCCTGTTCTGAAGGAACTATACCTCAG CTGGGGGTCCATCCTGGAGAGAGATGGCCACTATGCTATTGCAGCCAAGTGCTACTTAGGAGCCACTTCC GCCTATGATGCAGCCAAAGTTCTGGCCAGAAAGGGAGATGCAGCCTCACTTAGAACAGCTGCAGAGCTGG CTGCCATAGCAGGAGAACATGAGCTGGCTGCTTCCCTGGCTCTTAGATGTGCCCAAGAGCTGCTTCTGAT GAAGAACTGGGTGGGTGCACAAGAAGCATTGGGACTGCATGAAAGCCTGCAGGGCCAGAGACTGGTCTTC TGCCTCCTTGAACTTCTATGCCGGCACCTGGAGGAAAAACAGCCTCTAGAGGTCAGAGGCCCCTCTTCCA TTTACCACCAGTGGGCCACAGGTTCTGAGGGAACCTTGGTGCAGAGAGTAACCGGTGTGTGGCGCAGCGC CTTCAGTGTGGACACCCCGGAGCAGTGTCAGGCCGCCTTACAGAAGCTGCAGGATGTCAAGTACCCATCA GCAACAAGTAACACTCCCTTCAGACAGCTGCTGCTTCATGTCTGCCATGACCTGACCTTGGCAATGCTGA GCCAGCAGGCTGCTGCCTGGGAGGAGGCAGTGCCTGCTCTGCTGCAGGCTGTGGTTCGGAGCTATACCTC AGGGAACTTCACCCTCATGCAGGAAATCTACTCAGCCTTTCTCCCAGGTGGCTGTGACCACCTACGAGAC AAACTGGGTGACCTCTCTCCTGCCATGGCAGCTTACAAAAGCTTAGAGGCCTTTTGTATTTATGGGCAAC TGTATGAAGTCTGGTGGTCGCTCTGCGGGCCTGGCCCTGAGTCAAGCGTCTGGGTGTTGTCAGCTGAAAG CACGGTCTCTGATAAACAGAGCAAGCCAGAGGACAGTGCCAGTGCTGAGGACATGGAACAGCCTCCAGGC CCGGGCCCGAGGCTCAGTGCAGAGAGTGAGCGTTTGCTGAGTGCCTGCAAGGAGCTTTTCTCAGAACGGC ATGCCAGCCTGCAGACCTCCCAGAGGACTGTAGCCGAAGTCCAAGAGACACTGGCAGAAATGATCCGCCA GCACCAAAAGAGTCAGCTCTGCAAGGCCACAACAAATGGTCCCAGCAGAGATGAACCCAGCAGAGATGAA CCCAGCCAGGAAGCAGAGCGGGCTCCTTCTCAGCCTCCGAGCCCAACAGAAGAAAGAAATGCACCAGTGT CTCTCCCTGAACTAACCAGAAGGCTCACAGAGGCAAATGAGAGGATAGCAGAGTTTCCAGAGAGTGTAAA GGCCTGGCCCTTTCCAGATGTGCTGGAGTGCTGTCTCGTCTTGCTTCACATTGGGTCCCAGTGTCCTGAC GCTGTGGACCCTGAGATGCAGCAACAGGCCCAGGAGCTCCTTCACAAGTATGGGCACACTCGAGCTTACA GAAGACACTGCCAGAGTCGGCACACG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218608 protein sequence
Red=Cloning site Green=Tags(s) MKPEPRTLPPSPNWYCSRCSDAAPGGIFGFAARTSVFLVRVGPGAGASPGAPPFRVVGELVGHTERVSGF TFSHHPGQYNLCATSSDDGTVKVWDVETKTVVTEHTLHQHTISALHWSPTVKDLIVSGDEKGVVFCYWLN RNDSQHLFTEPRTIFCLTCSPHHENLVAIGYKDGIVVIIDISKKGEVIHRLRGHDDEIHSIAWCPLSGED CLSISQEENSEEPDIPNGKLIAETPITKGCYLATGSKDQTIRIWSCSRGRGVMVLKLPFLKRRSGGVDPT VKERLWLTLHWPKNQPTQLVSSCFGGELLLWDLTQSWRRKYTLFSTSAEGHNHSRIVFNLCSLKTEDGKQ LLLSTSMDRDVKCWDMATLECCWTLPSLGGFAYSLAFSPVDVGSLAIGVGDGMIRVWNTLSIKNNYDVKN FWQGVKSKVTALCWHPNKEGCLAFGTDDGKVGLYDTCSNKPPQISSTYHKKTVYRLAWGPPVPPMSLGGE GDRPSLTLYSCGGEGVVLQHNPWKLSGEAFDINKLVRDTNSIRYKLPVHTEISWKGDGKVLALGNEDGSI EIFQVPNLRLLCTIQQHHKLVNAIVWHHEHGSRPELSCLLASGSNNAVIYVHNLKAVLESNPESPITITE PYRTLSGHTAKITSLAWSPHHDGRLVSACYDGTAQVWDALREEPLFNFRGHRGRLLCVAWSPVDPECIYS GADDFCVYRWLTSMQDHSRPPQGKKCIELEKKRLSQFKPKLKKKKKPTLRLPVKQDSSVGNEDESVKENS GPAENGLSDQDGEEEAQEPELPPSPVVCVEPVSCTDICSGFEKSKVTVSSKATSLKKEPAKEKPEALLKK RKARSMLPLSTSLDHRSKEELHRDCLVLATATHAKAELNEDVSADLEERFHLGLFTDRATLYRMMETEGK GHLESGHPELFHQLMLWKGDLKGVLQAAAERGELTDSLVAVAPVAGYSVWLWAVEAFAKQLCFQDQYVKA ASYLLSIHKVYEAVELLKSNHLYREAIAVAKARLRPEDPVLKELYLSWGSILERDGHYAIAAKCYLGATS AYDAAKVLARKGDAASLRTAAELAAIAGEHELAASLALRCAQELLLMKNWVGAQEALGLHESLQGQRLVF CLLELLCRHLEEKQPLEVRGPSSIYHQWATGSEGTLVQRVTGVWRSAFSVDTPEQCQAALQKLQDVKYPS ATSNTPFRQLLLHVCHDLTLAMLSQQAAAWEEAVPALLQAVVRSYTSGNFTLMQEIYSAFLPGGCDHLRD KLGDLSPAMAAYKSLEAFCIYGQLYEVWWSLCGPGPESSVWVLSAESTVSDKQSKPEDSASAEDMEQPPG PGPRLSAESERLLSACKELFSERHASLQTSQRTVAEVQETLAEMIRQHQKSQLCKATTNGPSRDEPSRDE PSQEAERAPSQPPSPTEERNAPVSLPELTRRLTEANERIAEFPESVKAWPFPDVLECCLVLLHIGSQCPD AVDPEMQQQAQELLHKYGHTRAYRRHCQSRHT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172558 |
| ORF Size | 4506 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 6226 bp |
| RefSeq ORF | 4509 bp |
| Locus ID | 216766 |
| UniProt ID | Q8BX17 |
| MW | 166.6 kDa |
| Gene Summary | Required for the assembly of the SMN complex that plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. GEMIN5 acts as the snRNA-binding protein of the SMN complex. Binds to the 7-methylguanosine cap of RNA molecules (By similarity). Binds to the 3' UTR of SMN1 mRNA and regulates its translation; does not affect mRNA stability (PubMed:25911097). May play a role in the regulation of protein synthesis via its interaction with ribosomes (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224604 | Gemin5 (untagged) - Mouse gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant 2, (10ug) |
CNY 19,190.00 |
|
| MG218608 | Gemin5 (tGFP-tagged) - Mouse gem (nuclear organelle) associated protein 5 (Gemin5) transcript variant 2, (10ug) |
CNY 16,530.00 |
|
| MR218608L3 | Lenti ORF clone of Gemin5 (Myc-DDK-tagged) - Mouse gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant 2 |
CNY 16,910.00 |
|
| MR218608L4 | Lenti ORF clone of Gemin5 (mGFP-tagged) - Mouse gem (nuclear organelle) associated protein 5 (Gemin5), transcript variant 2 |
CNY 16,910.00 |
