United States
Germany
Japan
United Kingdom
China
Blm (NM_001042527) Mouse Untagged Clone
CAT＃: MC224423
Blm (untagged) - Mouse Bloom syndrome, RecQ helicase-like (Blm), transcript variant 2, (10ug)
CNY 18,240.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224423 representing NM_001042527
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCTGTTCCTCTGAACAATCTACAAGAACAACTACAGCGACACTCAGCCAGAAAACTTAATAATC AACCCAGCCTTTCAAAACCAAAATCTTTAGGTTTTACTTTTAAAAAGAAAACATCAGAGGGTGATGTGTC TGTCACTAGTGTGTCCGTAGTAAAAACACCTGCGTTAAGTGATAAAGATGTGAACGTGTCTGAGGCCTTT TCATTCACTGAGTCTCCACTCCACAAACCAAAGCAGCAGGCAAAGATTGAAGGCTTCTTTAAACATTTCC CTGGAAGGCAGCAAAGCAAGGGGACCTGCTCTGAGCCGTCACTGCCGGCCACGGTACAGACTGCTCAGGA CACTTTGTGCACTACCCCCAAAACCCCCACTGCGAAGAAACTGCCCGTGGCTGTTTTCAAGAAATTAGAA TTTAGTTCTTCTGCAGACTCCCTCAGTGACTGGGCTGATATGGATGACTTTGATATGTCAGCATCAGATG CGTTTGCTTCACTGGCTAAAAATCCTGCCACAAGAGTAAGCACCGCTCAGAAAATGAAAAAGACTAAGAG AAACTTCTTTAAACCACCACCTCGTAAAGCCAATGCAGTAAAGACTGACTTGACTCCTCCCTCCCCCGAA TGCCTGCAAGTGGATTTAACGAAGGAATCGGAGGAGGAGGAGGAGGAGGAGGAGGAGGCGGAGGGGGCGG ACTGCCTGAGCAGGGATGTGATCTGCATTGACAATGATTCTGCTTCTGAAGAGCTCACGGAGAAAGACAC GCAGGAAAGCCAGTCTTTGAAAGCTCACTTGGGAGCTGAAAGAGGTGACAGTGAAAAGAAGAGCCATGAA GACGAAGCTGTGTTCCATTCAGTTCAGAACACTGAATACTTTGAACACAATGACAATGATTATGATATAG ATTTTGTTCCACCTTCTCCAGAAGAAATCATCTCCACTGCTTCTTCCTCGTTGAAATGTTCCAGTATGTT AAAGGATCTTGATGACTCTGACAAAGAAAAGGGCATTCTTAGCACCTCAGAAGAGCTTCTGTCAAAACCA GAGGAAATGACCACACACAAGTCTGATGCAGGAACCAGTAAAGACTGTGATGCCCAGCAGATACGCATAC AGCAGCAGCTTATTCATGTGATGGAGCACATCTGTAAGTTAGTTGATACTGTTCCTACTGATGAACTGGA AGCTTTGAATTGTGGGACCGAATTGCTTCAACAACGAAACATAAGGAGGAAGCTCCTAGCTGAAGCAGGT TTTAATGGAAATGACGTCAGACTTCTGGGTTCTCTGTGGAGGCACAGGCCTGATTCACTTGATAACACAG TGCAGGGCGACTCCTGCCCTGTGGGGCATCCTAATAAAGAGTTAAATTCTCCATACCTTCTCTCACATTC CCCTTCCACTGAGGAATGTTTACCCACCACCACTCCAGGAAAGACAGGATTCTCAGCCACCCCGAAGAAT CTCTTTGAAAGGCCGTTATTGAATTCCCATTTACAGAAGTCCTTTGTAAGTAGCAACTGGGCTGAAACAC CAAGGATGGAAAACAGGAACGAAAGCACTGACTTCCCAGGGAGTGTTCTCACCAGCACCACTGTGAAAGC TCAGAGTAAACAAGCTGCTTCAGGATGGAACGTAGAGAGACACGGCCAGGCTTCCTATGATATCGATAAC TTTAATATTGATGACTTTGATGATGATGATGATGATGATGACTGGGAAAACATAATGCACAATTTTCCAG CCAGCAAATCTTCCACAGCCACCTACCCACCCATCAAGGAAGGTGGGCCAGTTAAATCTCTCTCAGAAAG GATTTCTTCAGCCAAGGCAAAGTTTCTTCCAGTGGTATCAACCGCTCAAAATACAAACCTCTCAGAGTCA ATTCAGAATTGCTCTGATAAGCTGGCCCAAAATTTATCATCAAAAAATCCAAAACATGAACATTTTCAAA GTCTTAATTTTCCTCATACAAAAGAAATGATGAAGATTTTCCATAAGAAATTTGGCTTGCATAATTTTAG AACTAATCAGCTAGAGGCGATCAATGCTGCGCTGCTTGGTGAAGACTGCTTTATCCTAATGCCCACTGGA GGAGGTAAAAGTTTGTGCTACCAGCTCCCTGCCTGTGTTTCTCCTGGGGTCACAATTGTCATTTCTCCCT TGAGATCACTAATAGTAGATCAAGTCCAAAAGCTGACTTCCTTTGATATTCCAGCTACATATCTGACAGG GGATAAGACTGACTCAGAAGCTGCAAATATTTACCTCCAATTATCCAAAAAAGACCCAATCATCAAGCTT TTGTATGTTACTCCAGAGAAGGTCTGTGCAAGTAACAGGCTGATTTCTACTCTGGAGAATCTGTATGAGC GGAAGCTCTTGGCACGTTTTGTCATTGATGAAGCGCATTGTGTGAGTCAGTGGGGTCATGATTTTCGTCA AGATTACAAAAGGATGAATATGCTTCGCCAGAAGTTTCCTTCTGTTCCAGTGATGGCCCTCACGGCCACA GCGAACCCCAGGGTCCAGAAGGACATCCTCACTCAGCTGAAGATCCTCAGACCTCAGGTGTTTAGCATGA GCTTTAACAGACACAATCTGAAGTACTATGTATTACCCAAGAAGCCCAAAAAAGTAGCATTTGATTGCCT AGAGTGGATCAGAAAGCATCACCCTTATGACTCGGGGATAATTTACTGCCTCTCCAGGAGGGAATGTGAC ACAATGGCTGACACTTTACAGAGAGAAGGCCTGGCTGCCCTGGCTTACCATGCGGGCCTCAGTGACTCTG CCAGAGATGAGGTGCAGCACAAGTGGATCAACCAGGACAACTGCCAGGTTATCTGTGCGACAATTGCGTT TGGAATGGGAATTGACAAACCTGACGTGCGATTTGTGATTCATGCATCTCTTCCTAAATCTATGGAGGGT TATTATCAAGAATCCGGCCGAGCTGGAAGAGATGGGGAAATATCTCACTGCGTGCTTTTCTATACATATC ATGATGTGACCAGACTGAAGAGACTTATAATGATGGAAAAAGATGGAAACTATCATACGAAGGAAACTCA CGTCAATAATCTATATAGCATGGTACATTACTGTGAAAACATAACGGAATGCAGAAGAATACAGCTTTTA GCTTACTTTGGTGAAAAAGGATTCAACCCTGATTTTTGTAAGAAATACCCAGATGTTTCTTGTGACAATT GCTGTAAAACAAAGGATTATAAAACAAAAGATGTGACTGATGACGTGAAAAATATTATAAGATTTGTTCA AGAACACAGTTCATCACCAGGAACAAGAAATATAGGACCTGCTGGAAGATTTACTCTGAACATGCTGGTC GACATTTTCTTGGGGAGCAAGAGTGCAAAAGTTAAGTCTGGAATATTTGGAAAGGGGACTACATATTCAC GACATAATGCCGAAAGACTTTTTAAAAAGCTGATTCTAGACAAAATCCTGGATGAAGACTTATATATCAA TGCCAATGACCAACCAATTGCCTATGTGATGCTAGGAACAAAAGCCCACAGTGTGCTGAGTGGCCACTTG AAGGTGGACTTCATGGAAACGGAAAATTCCAGCAGTATTAAAAAACAAAAAGCTTTAGTGGCCAAAGTAT CCCAGAGAGAAGAGGTAGTTAAGAAATGTCTTGGAGAACTTACAGAGGTCTGCAAATTGCTGGGGAAAGT CTTTGGTGTCCATTACTTCAATATTTTTAATACAGCCACACTCAAAAAGCTTGCAGAATCTTTATCTTCT GATCCTGAGGTTTTGCTTCAGATTGATGGTGTTACCGAAGACAAGCTGGAAAAATATGGTGCAGAAGTGA TTCCAGTATTACAGAAGTACTCAGAATGGACAGTGCCAGCTGAGGATGGTTCCCCAGGCGCCAGAGGCGC CCCAGAGGACACTGAGGAGGAGGAGGAGGAAGCGCCTGTATCTTCTCACTACTTTGCAAATCAAACTAGA AATGAAAGAAAGAGAAAGAAAATGTCAGCCACCCATAAGCCCAAGAGGAGAAGAACTAGTTACGGTGGCT TCAGAGCAAAGGGGGGCTCTACTACATGCAGAAAAACGACTTCTAAAAGTAAATTCTATGGTGTAACTGG ATCCCGCTCGGCCTCATGTGCTTCTCAGGCAACATCATCAGCCAGTAGAAAACTGGGGATTATGGCTCCT CCAAAGCCTGTAAATAGAACGTTCCTCAGGCCTTCATATGCCTTCTCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001042527 |
| Insert Size | 4251 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001042527.2, NP_001035992.1 |
| RefSeq Size | 4598 bp |
| RefSeq ORF | 4251 bp |
| Locus ID | 12144 |
| UniProt ID | O88700 |
| Gene Summary | ATP-dependent DNA helicase that unwinds single- and double-stranded DNA in a 3'-5' direction (PubMed:9840919). Participates in DNA replication and repair (By similarity). Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA (PubMed:9840919). Negatively regulates sister chromatid exchange (SCE) (PubMed:9840919, PubMed:27010503). Stimulates DNA 4-way junction branch migration and DNA Holliday junction dissolution. Binds single-stranded DNA (ssDNA), forked duplex DNA and DNA Holliday junction (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream translational start codon, compared to variant 1. The resulting isoform (2) is shorter at the N-terminus, compared to isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226518 | Blm (tGFP-tagged) - Mouse Bloom syndrome RecQ helicase-like (Blm) transcript variant 2, (10ug) |
CNY 11,880.00 |
|
| MR226518 | Blm (Myc-DDK-tagged) - Mouse Bloom syndrome, RecQ helicase-like (Blm), transcript variant 2 |
CNY 9,688.00 |
|
| MR226518L3 | Lenti ORF clone of Blm (Myc-DDK-tagged) - Mouse Bloom syndrome, RecQ helicase-like (Blm), transcript variant 2 |
CNY 12,730.00 |
|
| MR226518L4 | Lenti ORF clone of Blm (mGFP-tagged) - Mouse Bloom syndrome, RecQ helicase-like (Blm), transcript variant 2 |
CNY 12,730.00 |
