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Blm (NM_001042527) Mouse Tagged ORF Clone
CAT＃: MR226518
- TrueORF®
Blm (Myc-DDK-tagged) - Mouse Bloom syndrome, RecQ helicase-like (Blm), transcript variant 2
ORF Plasmid: tGFP
"NM_001042527" in other vectors (4)
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CNY 9,688.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226518 representing NM_001042527
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCTGTTCCTCTGAACAATCTACAAGAACAACTACAGCGACACTCAGCCAGAAAACTTAATAATC AACCCAGCCTTTCAAAACCAAAATCTTTAGGTTTTACTTTTAAAAAGAAAACATCAGAGGGTGATGTGTC TGTCACTAGTGTGTCCGTAGTAAAAACACCTGCGTTAAGTGATAAAGATGTGAACGTGTCTGAGGCCTTT TCATTCACTGAGTCTCCACTCCACAAACCAAAGCAGCAGGCAAAGATTGAAGGCTTCTTTAAACATTTCC CTGGAAGGCAGCAAAGCAAGGGGACCTGCTCTGAGCCGTCACTGCCGGCCACGGTACAGACTGCTCAGGA CACTTTGTGCACTACCCCCAAAACCCCCACTGCGAAGAAACTGCCCGTGGCTGTTTTCAAGAAATTAGAA TTTAGTTCTTCTGCAGACTCCCTCAGTGACTGGGCTGATATGGATGACTTTGATATGTCAGCATCAGATG CGTTTGCTTCACTGGCTAAAAATCCTGCCACAAGAGTAAGCACCGCTCAGAAAATGAAAAAGACTAAGAG AAACTTCTTTAAACCACCACCTCGTAAAGCCAATGCAGTAAAGACTGACTTGACTCCTCCCTCCCCCGAA TGCCTGCAAGTGGATTTAACGAAGGAATCGGAGGAGGAGGAGGAGGAGGAGGAGGAGGCGGAGGGGGCGG ACTGCCTGAGCAGGGATGTGATCTGCATTGACAATGATTCTGCTTCTGAAGAGCTCACGGAGAAAGACAC GCAGGAAAGCCAGTCTTTGAAAGCTCACTTGGGAGCTGAAAGAGGTGACAGTGAAAAGAAGAGCCATGAA GACGAAGCTGTGTTCCATTCAGTTCAGAACACTGAATACTTTGAACACAATGACAATGATTATGATATAG ATTTTGTTCCACCTTCTCCAGAAGAAATCATCTCCACTGCTTCTTCCTCGTTGAAATGTTCCAGTATGTT AAAGGATCTTGATGACTCTGACAAAGAAAAGGGCATTCTTAGCACCTCAGAAGAGCTTCTGTCAAAACCA GAGGAAATGACCACACACAAGTCTGATGCAGGAACCAGTAAAGACTGTGATGCCCAGCAGATACGCATAC AGCAGCAGCTTATTCATGTGATGGAGCACATCTGTAAGTTAGTTGATACTGTTCCTACTGATGAACTGGA AGCTTTGAATTGTGGGACCGAATTGCTTCAACAACGAAACATAAGGAGGAAGCTCCTAGCTGAAGCAGGT TTTAATGGAAATGACGTCAGACTTCTGGGTTCTCTGTGGAGGCACAGGCCTGATTCACTTGATAACACAG TGCAGGGCGACTCCTGCCCTGTGGGGCATCCTAATAAAGAGTTAAATTCTCCATACCTTCTCTCACATTC CCCTTCCACTGAGGAATGTTTACCCACCACCACTCCAGGAAAGACAGGATTCTCAGCCACCCCGAAGAAT CTCTTTGAAAGGCCGTTATTGAATTCCCATTTACAGAAGTCCTTTGTAAGTAGCAACTGGGCTGAAACAC CAAGGATGGAAAACAGGAACGAAAGCACTGACTTCCCAGGGAGTGTTCTCACCAGCACCACTGTGAAAGC TCAGAGTAAACAAGCTGCTTCAGGATGGAACGTAGAGAGACACGGCCAGGCTTCCTATGATATCGATAAC TTTAATATTGATGACTTTGATGATGATGATGATGATGATGACTGGGAAAACATAATGCACAATTTTCCAG CCAGCAAATCTTCCACAGCCACCTACCCACCCATCAAGGAAGGTGGGCCAGTTAAATCTCTCTCAGAAAG GATTTCTTCAGCCAAGGCAAAGTTTCTTCCAGTGGTATCAACCGCTCAAAATACAAACCTCTCAGAGTCA ATTCAGAATTGCTCTGATAAGCTGGCCCAAAATTTATCATCAAAAAATCCAAAACATGAACATTTTCAAA GTCTTAATTTTCCTCATACAAAAGAAATGATGAAGATTTTCCATAAGAAATTTGGCTTGCATAATTTTAG AACTAATCAGCTAGAGGCGATCAATGCTGCGCTGCTTGGTGAAGACTGCTTTATCCTAATGCCCACTGGA GGAGGTAAAAGTTTGTGCTACCAGCTCCCTGCCTGTGTTTCTCCTGGGGTCACAATTGTCATTTCTCCCT TGAGATCACTAATAGTAGATCAAGTCCAAAAGCTGACTTCCTTTGATATTCCAGCTACATATCTGACAGG GGATAAGACTGACTCAGAAGCTGCAAATATTTACCTCCAATTATCCAAAAAAGACCCAATCATCAAGCTT TTGTATGTTACTCCAGAGAAGGTCTGTGCAAGTAACAGGCTGATTTCTACTCTGGAGAATCTGTATGAGC GGAAGCTCTTGGCACGTTTTGTCATTGATGAAGCGCATTGTGTGAGTCAGTGGGGTCATGATTTTCGTCA AGATTACAAAAGGATGAATATGCTTCGCCAGAAGTTTCCTTCTGTTCCAGTGATGGCCCTCACGGCCACA GCGAACCCCAGGGTCCAGAAGGACATCCTCACTCAGCTGAAGATCCTCAGACCTCAGGTGTTTAGCATGA GCTTTAACAGACACAATCTGAAGTACTATGTATTACCCAAGAAGCCCAAAAAAGTAGCATTTGATTGCCT AGAGTGGATCAGAAAGCATCACCCTTATGACTCGGGGATAATTTACTGCCTCTCCAGGAGGGAATGTGAC ACAATGGCTGACACTTTACAGAGAGAAGGCCTGGCTGCCCTGGCTTACCATGCGGGCCTCAGTGACTCTG CCAGAGATGAGGTGCAGCACAAGTGGATCAACCAGGACAACTGCCAGGTTATCTGTGCGACAATTGCGTT TGGAATGGGAATTGACAAACCTGACGTGCGATTTGTGATTCATGCATCTCTTCCTAAATCTATGGAGGGT TATTATCAAGAATCCGGCCGAGCTGGAAGAGATGGGGAAATATCTCACTGCGTGCTTTTCTATACATATC ATGATGTGACCAGACTGAAGAGACTTATAATGATGGAAAAAGATGGAAACTATCATACGAAGGAAACTCA CGTCAATAATCTATATAGCATGGTACATTACTGTGAAAACATAACGGAATGCAGAAGAATACAGCTTTTA GCTTACTTTGGTGAAAAAGGATTCAACCCTGATTTTTGTAAGAAATACCCAGATGTTTCTTGTGACAATT GCTGTAAAACAAAGGATTATAAAACAAAAGATGTGACTGATGACGTGAAAAATATTATAAGATTTGTTCA AGAACACAGTTCATCACCAGGAACAAGAAATATAGGACCTGCTGGAAGATTTACTCTGAACATGCTGGTC GACATTTTCTTGGGGAGCAAGAGTGCAAAAGTTAAGTCTGGAATATTTGGAAAGGGGACTACATATTCAC GACATAATGCCGAAAGACTTTTTAAAAAGCTGATTCTAGACAAAATCCTGGATGAAGACTTATATATCAA TGCCAATGACCAACCAATTGCCTATGTGATGCTAGGAACAAAAGCCCACAGTGTGCTGAGTGGCCACTTG AAGGTGGACTTCATGGAAACGGAAAATTCCAGCAGTATTAAAAAACAAAAAGCTTTAGTGGCCAAAGTAT CCCAGAGAGAAGAGGTAGTTAAGAAATGTCTTGGAGAACTTACAGAGGTCTGCAAATTGCTGGGGAAAGT CTTTGGTGTCCATTACTTCAATATTTTTAATACAGCCACACTCAAAAAGCTTGCAGAATCTTTATCTTCT GATCCTGAGGTTTTGCTTCAGATTGATGGTGTTACCGAAGACAAGCTGGAAAAATATGGTGCAGAAGTGA TTCCAGTATTACAGAAGTACTCAGAATGGACAGTGCCAGCTGAGGATGGTTCCCCAGGCGCCAGAGGCGC CCCAGAGGACACTGAGGAGGAGGAGGAGGAAGCGCCTGTATCTTCTCACTACTTTGCAAATCAAACTAGA AATGAAAGAAAGAGAAAGAAAATGTCAGCCACCCATAAGCCCAAGAGGAGAAGAACTAGTTACGGTGGCT TCAGAGCAAAGGGGGGCTCTACTACATGCAGAAAAACGACTTCTAAAAGTAAATTCTATGGTGTAACTGG ATCCCGCTCGGCCTCATGTGCTTCTCAGGCAACATCATCAGCCAGTAGAAAACTGGGGATTATGGCTCCT CCAAAGCCTGTAAATAGAACGTTCCTCAGGCCTTCATATGCCTTCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226518 representing NM_001042527
Red=Cloning site Green=Tags(s) MAAVPLNNLQEQLQRHSARKLNNQPSLSKPKSLGFTFKKKTSEGDVSVTSVSVVKTPALSDKDVNVSEAF SFTESPLHKPKQQAKIEGFFKHFPGRQQSKGTCSEPSLPATVQTAQDTLCTTPKTPTAKKLPVAVFKKLE FSSSADSLSDWADMDDFDMSASDAFASLAKNPATRVSTAQKMKKTKRNFFKPPPRKANAVKTDLTPPSPE CLQVDLTKESEEEEEEEEEAEGADCLSRDVICIDNDSASEELTEKDTQESQSLKAHLGAERGDSEKKSHE DEAVFHSVQNTEYFEHNDNDYDIDFVPPSPEEIISTASSSLKCSSMLKDLDDSDKEKGILSTSEELLSKP EEMTTHKSDAGTSKDCDAQQIRIQQQLIHVMEHICKLVDTVPTDELEALNCGTELLQQRNIRRKLLAEAG FNGNDVRLLGSLWRHRPDSLDNTVQGDSCPVGHPNKELNSPYLLSHSPSTEECLPTTTPGKTGFSATPKN LFERPLLNSHLQKSFVSSNWAETPRMENRNESTDFPGSVLTSTTVKAQSKQAASGWNVERHGQASYDIDN FNIDDFDDDDDDDDWENIMHNFPASKSSTATYPPIKEGGPVKSLSERISSAKAKFLPVVSTAQNTNLSES IQNCSDKLAQNLSSKNPKHEHFQSLNFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTG GGKSLCYQLPACVSPGVTIVISPLRSLIVDQVQKLTSFDIPATYLTGDKTDSEAANIYLQLSKKDPIIKL LYVTPEKVCASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTAT ANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECD TMADTLQREGLAALAYHAGLSDSARDEVQHKWINQDNCQVICATIAFGMGIDKPDVRFVIHASLPKSMEG YYQESGRAGRDGEISHCVLFYTYHDVTRLKRLIMMEKDGNYHTKETHVNNLYSMVHYCENITECRRIQLL AYFGEKGFNPDFCKKYPDVSCDNCCKTKDYKTKDVTDDVKNIIRFVQEHSSSPGTRNIGPAGRFTLNMLV DIFLGSKSAKVKSGIFGKGTTYSRHNAERLFKKLILDKILDEDLYINANDQPIAYVMLGTKAHSVLSGHL KVDFMETENSSSIKKQKALVAKVSQREEVVKKCLGELTEVCKLLGKVFGVHYFNIFNTATLKKLAESLSS DPEVLLQIDGVTEDKLEKYGAEVIPVLQKYSEWTVPAEDGSPGARGAPEDTEEEEEEAPVSSHYFANQTR NERKRKKMSATHKPKRRRTSYGGFRAKGGSTTCRKTTSKSKFYGVTGSRSASCASQATSSASRKLGIMAP PKPVNRTFLRPSYAFS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001042527 |
| ORF Size | 4248 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001042527.2, NP_001035992.1 |
| RefSeq Size | 4598 bp |
| RefSeq ORF | 4251 bp |
| Locus ID | 12144 |
| UniProt ID | O88700 |
| MW | 158.8 kDa |
| Gene Summary | ATP-dependent DNA helicase that unwinds single- and double-stranded DNA in a 3'-5' direction (PubMed:9840919). Participates in DNA replication and repair (By similarity). Involved in 5'-end resection of DNA during double-strand break (DSB) repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA (PubMed:9840919). Negatively regulates sister chromatid exchange (SCE) (PubMed:9840919, PubMed:27010503). Stimulates DNA 4-way junction branch migration and DNA Holliday junction dissolution. Binds single-stranded DNA (ssDNA), forked duplex DNA and DNA Holliday junction (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224423 | Blm (untagged) - Mouse Bloom syndrome, RecQ helicase-like (Blm), transcript variant 2, (10ug) |
CNY 18,240.00 |
|
| MG226518 | Blm (tGFP-tagged) - Mouse Bloom syndrome RecQ helicase-like (Blm) transcript variant 2, (10ug) |
CNY 11,880.00 |
|
| MR226518L3 | Lenti ORF clone of Blm (Myc-DDK-tagged) - Mouse Bloom syndrome, RecQ helicase-like (Blm), transcript variant 2 |
CNY 12,730.00 |
|
| MR226518L4 | Lenti ORF clone of Blm (mGFP-tagged) - Mouse Bloom syndrome, RecQ helicase-like (Blm), transcript variant 2 |
CNY 12,730.00 |
