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Erbb2 (NM_001003817) Mouse Untagged Clone
CAT＃: MC224084
Erbb2 (untagged) - Mouse v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (Erbb2), (10ug)
CNY 13,128.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | c-erbB2; c-neu; Erbb-2; HER-2; HER2; l11Jus8; mKIAA3023; Neu |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224084 representing NM_001003817
Red=Cloning site Blue=ORF TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGGCGGCCTGGTGCCGTTGGGGGTTCCTCCTCGCCCTCCTGTCCCCCGGAGCCGCGGGTACCC AAGTGTGTACCGGTACCGACATGAAGTTGCGACTCCCTGCCAGTCCTGAGACCCACCTGGACATGCTTCG CCACCTCTACCAGGGCTGTCAGGTGGTGCAGGGCAATTTGGAGCTTACCTACCTGCCCGCCAATGCCAGC CTCTCATTCCTGCAGGACATCCAGGAAGTCCAGGGATACATGCTCATCGCTCACAACCGAGTGAAACACG TCCCACTGCAGAGGTTGCGCATCGTGAGAGGGACTCAGCTCTTTGAGGACAAGTATGCCCTGGCTGTGCT AGACAACCGAGACCCTTTGGACAACGTCACCACCGCCGCCCCAGGCAGAACCCCAGAAGGGCTGCGGGAG CTGCAGCTTCGAAGTCTCACAGAGATCTTGAAGGGAGGAGTTTTGATCCGTGGGAACCCTCAGCTCTGCT ACCAGGACATGGTTTTGTGGAAGGATGTCCTCCGTAAGAATAACCAGCTGGCTCCTGTCGACATGGACAC CAATCGTTCCCGGGCCTGTCCACCTTGTGCCCCAACCTGCAAAGACAATCACTGTTGGGGTGAGAGTCCT GAAGACTGTCAGATCTTGACTGGCACCATCTGTACTAGTGGCTGTGCCCGGTGCAAGGGCCGGCTGCCCA CTGACTGTTGCCATGAGCAGTGTGCTGCAGGCTGCACGGGTCCCAAGCATTCTGACTGCCTGGCCTGCCT CCACTTCAATCATAGTGGTATCTGTGAGCTGCACTGCCCGGCCCTCATCACCTACAACACAGACACCTTC GAGTCCATGCTCAACCCTGAGGGTCGCTACACCTTTGGTGCCAGCTGTGTGACCACCTGCCCCTACAACT ACCTCTCCACGGAAGTGGGATCCTGCACTCTGGTCTGTCCCCCGAACAACCAAGAGGTCACAGCTGAGGA CGGAACACAGCGGTGTGAGAAATGCAGCAAGCCCTGTGCTGGAGTATGCTATGGTCTGGGCATGGAGCAC CTCCGAGGGGCGAGGGCCATCACCAGTGACAATATCCAGGAGTTTGCTGGCTGCAAGAAGATCTTTGGGA GCCTGGCATTTTTGCCGGAGAGCTTTGATGGGAACCCCTCCTCCGGCGTTGCCCCACTGAAGCCAGAGCA TCTCCAAGTGTTCGAAACCCTGGAGGAGATCACAGGTTACCTATACATTTCAGCATGGCCAGAGAGCTTC CAAGACCTCAGTGTCTTCCAGAACCTTCGGGTCATTCGGGGACGGATTCTCCATGATGGTGCTTACTCAT TGACGTTGCAAGGCCTGGGGATTCACTCACTGGGGCTACGCTCACTGCGGGAGCTGGGCAGTGGATTGGC TCTCATTCACCGCAACACCCATCTCTGCTTTGTAAACACTGTACCTTGGGACCAGCTCTTCCGGAACCCG CACCAGGCCCTACTCCACAGTGGGAACCGGCCAGAAGAGGCATGTGGTCTTGAGGGCTTGGTCTGTAACT CACTGTGTGCCCGTGGGCACTGCTGGGGGCCAGGGCCCACCCAGTGTGTCAACTGCAGTCAGTTCCTCCG GGGCCAGGAGTGTGTGGAGGAGTGCCGAGTATGGAAGGGGCTCCCCAGGGAGTATGTGAGGGGCAAGCAC TGTCTGCCATGCCACCCCGAGTGTCAGCCTCAAAACAGCTCGGAGACCTGCTATGGATCGGAGGCTGACC AGTGTGAGGCTTGTGCCCACTACAAGGACTCATCTTCCTGTGTGGCTCGCTGCCCCAGTGGTGTGAAGCC AGACCTCTCCTACATGCCTATCTGGAAGTACCCGGATGAGGAGGGCATATGTCAGCCATGCCCCATCAAC TGCACCCACTCATGTGTGGACCTGGACGAACGAGGCTGCCCAGCAGAGCAGAGAGCCAGCCCAGTGACAT TCATCATTGCAACTGTGGTGGGCGTCCTGTTGTTCCTGATCATAGTGGTGGTCATTGGAATCCTAATCAA ACGAAGGCGACAGAAGATCCGGAAGTATACCATGCGTAGGCTGCTGCAGGAGACCGAGCTGGTGGAGCCG CTGACGCCCAGTGGAGCTGTGCCCAACCAGGCTCAGATGCGGATCCTAAAGGAGACAGAGCTAAGGAAGC TGAAGGTGCTTGGGTCAGGAGCCTTCGGCACTGTCTACAAGGGCATCTGGATCCCAGATGGGGAGAACGT GAAAATCCCCGTGGCCATCAAGGTGTTGAGGGAAAACACATCTCCTAAAGCTAACAAAGAAATCCTAGAT GAAGCGTACGTCATGGCTGGTGTGGGTTCTCCATATGTGTCCCGCCTCCTGGGCATCTGCCTGACATCCA CAGTGCAGCTGGTGACACAGCTTATGCCCTATGGCTGCCTTCTGGACCATGTCCGAGAACACCGAGGTCG CTTAGGCTCCCAGGACCTGCTCAACTGGTGTGTTCAGATTGCCAAGGGGATGAGCTACCTGGAGGAAGTT CGGCTTGTTCACAGGGACCTAGCTGCCCGAAACGTGCTAGTCAAGAGTCCCAACCACGTCAAGATTACCG ACTTCGGGCTGGCACGGCTGCTGGACATTGATGAGACTGAATACCATGCAGATGGGGGCAAGGTGCCCAT CAAGTGGATGGCATTGGAATCTATTCTCAGACGCCGGTTCACCCATCAGAGTGATGTGTGGAGCTATGGT GTGACTGTGTGGGAGCTGATGACCTTTGGGGCCAAACCTTACGATGGGATCCCAGCTCGGGAGATCCCTG ATTTGCTGGAGAAGGGAGAACGCCTACCTCAGCCTCCAATCTGCACCATCGACGTCTACATGATCATGGT CAAATGTTGGATGATTGACTCCGAATGTCGCCCGAGATTCCGGGAGTTGGTATCAGAATTCTCCCGTATG GCAAGGGACCCCCAGCGCTTTGTGGTCATCCAGAACGAGGACTTAGGCCCCTCCAGCCCCATGGACAGCA CCTTCTACCGTTCACTGCTGGAGGATGATGACATGGGGGAGCTGGTCGATGCTGAAGAGTACCTGGTACC CCAGCAGGGATTCTTCTCCCCAGACCCTGCCCTAGGTACTGGGAGCACAGCCCACCGCAGACACCGCAGC TCGTCGGCCAGGAGTGGCGGTGGTGAGCTGACACTGGGCCTGGAGCCCTCGGAAGAAGAGCCCCCCAGAT CTCCACTGGCTCCCTCCGAAGGGGCTGGCTCCGATGTGTTTGATGGTGACCTGGCAGTGGGGGTAACCAA AGGACTGCAGAGCCTCTCTCCACATGACCTCAGCCCTCTACAGCGGTACAGTGAGGATCCCACATTACCT CTGCCCCCCGAGACTGATGGCTACGTTGCTCCCCTGGCCTGCAGCCCCCAGCCCGAGTATGTGAACCAGC CAGAGGTTCGGCCTCAGTCTCCCTTGACCCCAGAGGGTCCTCCGCCTCCCATCCGACCTGCTGGTGCTAC TCTAGAAAGACCCAAGACTCTCTCTCCTGGGAAAAATGGGGTTGTCAAAGACGTTTTTGCCTTTGGGGGT GCTGTGGAGAACCCTGAATACTTAGCACCCAGAGCAGGCACTGCCTCTCAGCCCCACCCTTCTCCTGCCT TCAGCCCAGCCTTTGACAACCTCTATTACTGGGACCAGAACTCATCGGAGCAGGGTCCTCCACCAAGTAC CTTTGAAGGGACCCCCACTGCAGAGAACCCTGAGTACCTAGGCCTGGATGTGCCAGTATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001003817 |
| Insert Size | 3771 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001003817.1, NP_001003817.1 |
| RefSeq Size | 4998 bp |
| RefSeq ORF | 3771 bp |
| Locus ID | 13866 |
| UniProt ID | P70424 |
| Gene Summary | Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG227307 | Erbb2 (tGFP-tagged) - Mouse v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 neuro/glioblastoma derived oncogene homolog (avian) (Erbb2), (10ug) |
CNY 13,780.00 |
|
| MR227307 | Erbb2 (Myc-DDK-tagged) - Mouse v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (Erbb2) |
CNY 13,120.00 |
|
| MR227307L3 | Lenti ORF clone of Erbb2 (Myc-DDK-tagged) - Mouse v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (Erbb2) |
CNY 14,440.00 |
|
| MR227307L4 | Lenti ORF clone of Erbb2 (mGFP-tagged) - Mouse v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (Erbb2) |
CNY 14,440.00 |
