Phka1 (NM_173021) Mouse Untagged Clone
CAT#: MC223856
Phka1 (untagged) - Mouse phosphorylase kinase alpha 1 (Phka1), transcript variant 2, (10ug)
CNY 15,200.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 5330411D17; 9830108K24Rik; Phka |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC223856 representing NM_173021
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAGCCGCAGTAACTCGGGAGTCCGCCTGGACGGCTATGCTCGGCTGGTGCATCAGACCATCCTGT GCCATCAGAATCCAGTGACAGGCTTGCTTCCAGCCAGCTATGATCAAAAAGATGCCTGGGTCCGAGATAA TGTGTACAGTATCCTGGCTGTGTGGGGTTTGGGCCTGGCATATCGCAAAAATGCTGACCGTGATGAGGAC AAGGCAAAGGCCTATGAACTGGAGCAGAGTGTAGTGAAGTTAATGAGGGGACTGCTGCACTGCATGATCA GACAGGTGGATAAAGTAGAGTCCTTCAAGTACAGTCAGAGTACTAAAGATAGCCTCCATGCCAAGTACAA CACCAAAACTTGTGCCACTGTGGTGGGTGATGACCAGTGGGGACACCTGCAGTTGGATGCTACTTCTGTG TACCTGCTCTTCCTAGCACAAATGACTGCCTCAGGACTCCATATCATCCACAGCCTAGACGAAGTCAATT TTATACAGAACCTCGTGTTTTACATTGAAGCTGCATATAAAACTGCTGACTTTGGGATATGGGAACGTGG CGATAAGACAAACCAAGGCATCTCGGAATTGAATGCGAGTTCAGTTGGAATGGCCAAGGCAGCCCTGGAA GCACTAGATGAATTAGACTTGTTTGGTGTGAAAGGTGGGCCACAATCAGTTATCCATGTCCTGGCTGATG AAGTCCAACACTGCCAGTCTATCCTGAATTCACTACTGCCCAGGGCTTCAACATCCAAAGAAGTTGATGC CAGTCTGCTCTCAGTGGTCTCTTTCCCAGCCTTTGCTGTAGAGGACAGCCATTTGGTGGAGCTCACCAAA CAGGAGATCATCACCAAGCTTCAGGGTCGTTATGGTTGCTGTCGTTTTCTGCGAGATGGATATAAAACTC CTAAAGAGGATCCCAATCGCCTATACTATGAACCAGCTGAGCTGAAGCTATTTGAAAACATTGAGTGCGA ATGGCCATTGTTCTGGACATACTTTATCCTTGATGGGATCTTCAGTGGCAACGTAGAACAGGTTCAAGAA TATAGAGAGGCTCTTGATGCAGTCCTCATCAAGGGCAAAAATGGAGTCCCTCTTCTTCCAGAGCTGTACA GTGTCCCTCCTGACAGGGTTGATGAAGAGTATCAAAATCCCCACACTGTGGATCGAGTCCCTATGGGAAA ATTGCCTCACATGTGGGGTCAGTCTCTATACATTTTAGGAAGCTTGATGGCAGAGGGATTTTTAGCTCCT GGAGAAATTGATCCCCTGAATCGTAGGTTTTCTACTGTGCCAAAGCCAGATGTGGTGGTTCAAGTCTCCA TTCTGGCTGAAACAGAAGAAATCAAGGCCATTTTGAAGGACAAAGGAATTGATGTGGAGACCATTGCTGA AGTGTACCCCATAAGAGTACAGCCAGCTCGTATTCTCAGCCATATTTATTCTAGTCTAGGATGCAACAGT AGAATGAAACTCAGTGGACGACCCTACAGGCTCATGGGTGTGCTTGGAACATCAAAACTTTATGACATTC GCAAAACTATCTTTACTTTCACTCCACAGTTTATAGACCAGCAACAGTTCTACCTGGCTCTGGACAACCA GATGATAGTAGAAATGCTCAGAACAGACCTTTCCTACCTCTGTAGCCGCTGGAGGATGACAGGCCAGCCC ACGATCACTTTCCCTATCTCGCACACCATGCTTGATGAAGATGGAACCAGCTTGAATTCAAGTATCTTGG CAGCACTCCGAAAAATGCAGGATGGCTATTTTGGTGGGGCCAGGATCCAAACAGGTAAATTGTCAGAGTT TTTGACAACATCTTGCTGCACACACTTAAGCTTCATGGACCCTGGACCTGAGGGTAAGCTGTACAGTGAA GATTATGATGAAGACTATGAAGATGATTTGGACTCTGGCAACTGGATGGACAGCTATGATTCAACAAGTA ATGATGTTCACATGTATCTTCCTACAAAGTTATTTCAGCCTTCTCGTCCTTCACTCAACTTACTTGACTC CCCTGAGTCTCCACAAGATAGCCAGGTTCCTTCCGTTCATGTAGAAGTGCATCTTCCTAGGGACCAGTCT GGGGAAGTGGACTTCCAGTCATTGGTTTCACAGTTGAAGGAGACCTCAAGCTTACAGGAGCAAGCTGATA TACTCTACATGCTGTATTCAATGAAAGGACCTGACTGGAACACTGAATTGTATGAAGAAGGGGGGGCTAC TGTCAGAGAGCTTCTTAGTGAACTGTATGTCAAAGTTGGTGAAATTCGGCACTGGGGTCTGATCCGATAT ATCTCTGGGATCTTACGGAAGAAAGTGGAGGCACTTGATGAGGCCTGCACAGACCTTCTGTCCTACCAGA AACACCTGACAGTAGGACTTCCTCCAGAACCTCGAGAGAAGACCATCTCTGCGCCTCTACCGTACGAGGC ACTCACTAAGCTGATAGATGAAGCCAGTGAAGGCGACATGAGCATCTCAACCCTTACACAGGAAATAATG GTCTATCTTGCCATGTATATGAGAACTCAGCCTGGCCTCTTTGCAGAAATGTTCAGACTTCGAATCGGTT TGATCATACAAGTTATGGCAACAGAACTAGCACACTCTCTTCGATGTTCAGCTGAGGAAGCCACAGAGGG CCTGATGAATCTCAGTCCTTCAGCCATGAAGAACCTCCTGCATCACATTCTCAGTGGCAAAGAGTTTGGA GTGGAACGAAGCGTTCGTCCCACTGATTCAAATGTGAGTCCTGCTATTTCCATCCATGAGATTGGTGCTG TTGGAGCAACAAAAACTGAACGAACTGGAATCATGCAGTTAAAAAGTGAGATAAAGCAGGTGGAATTTCG TAGGCTGTCTGTCTCGATGGAGAGTCAGACTAGTGGTGGTCATCCCTCGGGTGTAGATTTGATGTCGCCA TCCTTTCTGTCCCCTGCAGCCTGTATTGCTGCAAGTAGTGGATCCTTTCCTACGGTGTGTGATCATCAGA CATCTAAAGATAGTCGTCAAGGCCAGTGGCAACGCAGGAGAAGGCTAGATGGAGCACTAAATAGAGTACC AATTGGATTTTATCAAAAAGTATGGAAAATTTTGCAGAAATGTCATGGGCTTTCTGTGGAAGGTTTTGTT CTTCCCTCTTCAACCACTAGGGAGATGACCCCAGGTGAGATTAAATTCTCTGTCCATGTGGAGTCTGTCC TGAATCGTGTCCCTCAGCCAGAATACCGCCAACTTCTGGTTGAAGCCATCCTTGTTCTCACCATGCTGGC AGATATTGAAATTCATAGCATTGGGAGCATCATTGCTGTGGAGAAAATAGTTCATATTGCCAACGACTTG TTCCTTCAAGAACAGAAAACCCTCGGCGCAGATGATACCATGTTGGCAAAGGATCCTGCATCTGGCATCT GTACTCTCCTGTATGACAGTGCACCCAGTGGCAGATTTGGCACCATGACCTACCTCTCCAAGGCAGCTGC CACCTACGTGCAGGAGTTTCTGCCACACAGCCTCTGTGCCATGCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_173021 |
Insert Size | 3549 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_173021.3, NP_766609.2 |
RefSeq Size | 5938 bp |
RefSeq ORF | 3549 bp |
Locus ID | 18679 |
Gene Summary | Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG226481 | Phka1 (tGFP-tagged) - Mouse phosphorylase kinase alpha 1 (Phka1) transcript variant 2, (10ug) |
CNY 11,400.00 |
|
MR226481 | Phka1 (Myc-DDK-tagged) - Mouse phosphorylase kinase alpha 1 (Phka1), transcript variant 2 |
CNY 8,232.00 |
|
MR226481L3 | Lenti ORF clone of Phka1 (Myc-DDK-tagged) - Mouse phosphorylase kinase alpha 1 (Phka1), transcript variant 2 |
CNY 12,260.00 |
|
MR226481L4 | Lenti ORF clone of Phka1 (mGFP-tagged) - Mouse phosphorylase kinase alpha 1 (Phka1), transcript variant 2 |
CNY 12,260.00 |