United States
Germany
Japan
United Kingdom
China
Phka1 (NM_173021) Mouse Tagged ORF Clone
CAT＃: MR226481
- TrueORF®
Phka1 (Myc-DDK-tagged) - Mouse phosphorylase kinase alpha 1 (Phka1), transcript variant 2
ORF Plasmid: tGFP
"NM_173021" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 8,232.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5330411D17; 9830108K24Rik; Phka |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226481 representing NM_173021
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGAGCCGCAGTAACTCGGGAGTCCGCCTGGACGGCTATGCTCGGCTGGTGCATCAGACCATCCTGT GCCATCAGAATCCAGTGACAGGCTTGCTTCCAGCCAGCTATGATCAAAAAGATGCCTGGGTCCGAGATAA TGTGTACAGTATCCTGGCTGTGTGGGGTTTGGGCCTGGCATATCGCAAAAATGCTGACCGTGATGAGGAC AAGGCAAAGGCCTATGAACTGGAGCAGAGTGTAGTGAAGTTAATGAGGGGACTGCTGCACTGCATGATCA GACAGGTGGATAAAGTAGAGTCCTTCAAGTACAGTCAGAGTACTAAAGATAGCCTCCATGCCAAGTACAA CACCAAAACTTGTGCCACTGTGGTGGGTGATGACCAGTGGGGACACCTGCAGTTGGATGCTACTTCTGTG TACCTGCTCTTCCTAGCACAAATGACTGCCTCAGGACTCCATATCATCCACAGCCTAGACGAAGTCAATT TTATACAGAACCTCGTGTTTTACATTGAAGCTGCATATAAAACTGCTGACTTTGGGATATGGGAACGTGG CGATAAGACAAACCAAGGCATCTCGGAATTGAATGCGAGTTCAGTTGGAATGGCCAAGGCAGCCCTGGAA GCACTAGATGAATTAGACTTGTTTGGTGTGAAAGGTGGGCCACAATCAGTTATCCATGTCCTGGCTGATG AAGTCCAACACTGCCAGTCTATCCTGAATTCACTACTGCCCAGGGCTTCAACATCCAAAGAAGTTGATGC CAGTCTGCTCTCAGTGGTCTCTTTCCCAGCCTTTGCTGTAGAGGACAGCCATTTGGTGGAGCTCACCAAA CAGGAGATCATCACCAAGCTTCAGGGTCGTTATGGTTGCTGTCGTTTTCTGCGAGATGGATATAAAACTC CTAAAGAGGATCCCAATCGCCTATACTATGAACCAGCTGAGCTGAAGCTATTTGAAAACATTGAGTGCGA ATGGCCATTGTTCTGGACATACTTTATCCTTGATGGGATCTTCAGTGGCAACGTAGAACAGGTTCAAGAA TATAGAGAGGCTCTTGATGCAGTCCTCATCAAGGGCAAAAATGGAGTCCCTCTTCTTCCAGAGCTGTACA GTGTCCCTCCTGACAGGGTTGATGAAGAGTATCAAAATCCCCACACTGTGGATCGAGTCCCTATGGGAAA ATTGCCTCACATGTGGGGTCAGTCTCTATACATTTTAGGAAGCTTGATGGCAGAGGGATTTTTAGCTCCT GGAGAAATTGATCCCCTGAATCGTAGGTTTTCTACTGTGCCAAAGCCAGATGTGGTGGTTCAAGTCTCCA TTCTGGCTGAAACAGAAGAAATCAAGGCCATTTTGAAGGACAAAGGAATTGATGTGGAGACCATTGCTGA AGTGTACCCCATAAGAGTACAGCCAGCTCGTATTCTCAGCCATATTTATTCTAGTCTAGGATGCAACAGT AGAATGAAACTCAGTGGACGACCCTACAGGCTCATGGGTGTGCTTGGAACATCAAAACTTTATGACATTC GCAAAACTATCTTTACTTTCACTCCACAGTTTATAGACCAGCAACAGTTCTACCTGGCTCTGGACAACCA GATGATAGTAGAAATGCTCAGAACAGACCTTTCCTACCTCTGTAGCCGCTGGAGGATGACAGGCCAGCCC ACGATCACTTTCCCTATCTCGCACACCATGCTTGATGAAGATGGAACCAGCTTGAATTCAAGTATCTTGG CAGCACTCCGAAAAATGCAGGATGGCTATTTTGGTGGGGCCAGGATCCAAACAGGTAAATTGTCAGAGTT TTTGACAACATCTTGCTGCACACACTTAAGCTTCATGGACCCTGGACCTGAGGGTAAGCTGTACAGTGAA GATTATGATGAAGACTATGAAGATGATTTGGACTCTGGCAACTGGATGGACAGCTATGATTCAACAAGTA ATGATGTTCACATGTATCTTCCTACAAAGTTATTTCAGCCTTCTCGTCCTTCACTCAACTTACTTGACTC CCCTGAGTCTCCACAAGATAGCCAGGTTCCTTCCGTTCATGTAGAAGTGCATCTTCCTAGGGACCAGTCT GGGGAAGTGGACTTCCAGTCATTGGTTTCACAGTTGAAGGAGACCTCAAGCTTACAGGAGCAAGCTGATA TACTCTACATGCTGTATTCAATGAAAGGACCTGACTGGAACACTGAATTGTATGAAGAAGGGGGGGCTAC TGTCAGAGAGCTTCTTAGTGAACTGTATGTCAAAGTTGGTGAAATTCGGCACTGGGGTCTGATCCGATAT ATCTCTGGGATCTTACGGAAGAAAGTGGAGGCACTTGATGAGGCCTGCACAGACCTTCTGTCCTACCAGA AACACCTGACAGTAGGACTTCCTCCAGAACCTCGAGAGAAGACCATCTCTGCGCCTCTACCGTACGAGGC ACTCACTAAGCTGATAGATGAAGCCAGTGAAGGCGACATGAGCATCTCAACCCTTACACAGGAAATAATG GTCTATCTTGCCATGTATATGAGAACTCAGCCTGGCCTCTTTGCAGAAATGTTCAGACTTCGAATCGGTT TGATCATACAAGTTATGGCAACAGAACTAGCACACTCTCTTCGATGTTCAGCTGAGGAAGCCACAGAGGG CCTGATGAATCTCAGTCCTTCAGCCATGAAGAACCTCCTGCATCACATTCTCAGTGGCAAAGAGTTTGGA GTGGAACGAAGCGTTCGTCCCACTGATTCAAATGTGAGTCCTGCTATTTCCATCCATGAGATTGGTGCTG TTGGAGCAACAAAAACTGAACGAACTGGAATCATGCAGTTAAAAAGTGAGATAAAGCAGGTGGAATTTCG TAGGCTGTCTGTCTCGATGGAGAGTCAGACTAGTGGTGGTCATCCCTCGGGTGTAGATTTGATGTCGCCA TCCTTTCTGTCCCCTGCAGCCTGTATTGCTGCAAGTAGTGGATCCTTTCCTACGGTGTGTGATCATCAGA CATCTAAAGATAGTCGTCAAGGCCAGTGGCAACGCAGGAGAAGGCTAGATGGAGCACTAAATAGAGTACC AATTGGATTTTATCAAAAAGTATGGAAAATTTTGCAGAAATGTCATGGGCTTTCTGTGGAAGGTTTTGTT CTTCCCTCTTCAACCACTAGGGAGATGACCCCAGGTGAGATTAAATTCTCTGTCCATGTGGAGTCTGTCC TGAATCGTGTCCCTCAGCCAGAATACCGCCAACTTCTGGTTGAAGCCATCCTTGTTCTCACCATGCTGGC AGATATTGAAATTCATAGCATTGGGAGCATCATTGCTGTGGAGAAAATAGTTCATATTGCCAACGACTTG TTCCTTCAAGAACAGAAAACCCTCGGCGCAGATGATACCATGTTGGCAAAGGATCCTGCATCTGGCATCT GTACTCTCCTGTATGACAGTGCACCCAGTGGCAGATTTGGCACCATGACCTACCTCTCCAAGGCAGCTGC CACCTACGTGCAGGAGTTTCTGCCACACAGCCTCTGTGCCATGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226481 representing NM_173021
Red=Cloning site Green=Tags(s) MRSRSNSGVRLDGYARLVHQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLAYRKNADRDED KAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSV YLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALE ALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSLLPRASTSKEVDASLLSVVSFPAFAVEDSHLVELTK QEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGIFSGNVEQVQE YREALDAVLIKGKNGVPLLPELYSVPPDRVDEEYQNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAP GEIDPLNRRFSTVPKPDVVVQVSILAETEEIKAILKDKGIDVETIAEVYPIRVQPARILSHIYSSLGCNS RMKLSGRPYRLMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNQMIVEMLRTDLSYLCSRWRMTGQP TITFPISHTMLDEDGTSLNSSILAALRKMQDGYFGGARIQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSE DYDEDYEDDLDSGNWMDSYDSTSNDVHMYLPTKLFQPSRPSLNLLDSPESPQDSQVPSVHVEVHLPRDQS GEVDFQSLVSQLKETSSLQEQADILYMLYSMKGPDWNTELYEEGGATVRELLSELYVKVGEIRHWGLIRY ISGILRKKVEALDEACTDLLSYQKHLTVGLPPEPREKTISAPLPYEALTKLIDEASEGDMSISTLTQEIM VYLAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATEGLMNLSPSAMKNLLHHILSGKEFG VERSVRPTDSNVSPAISIHEIGAVGATKTERTGIMQLKSEIKQVEFRRLSVSMESQTSGGHPSGVDLMSP SFLSPAACIAASSGSFPTVCDHQTSKDSRQGQWQRRRRLDGALNRVPIGFYQKVWKILQKCHGLSVEGFV LPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMLADIEIHSIGSIIAVEKIVHIANDL FLQEQKTLGADDTMLAKDPASGICTLLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSLCAMQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_173021 |
| ORF Size | 3546 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_173021.3, NP_766609.2 |
| RefSeq Size | 5938 bp |
| RefSeq ORF | 3549 bp |
| Locus ID | 18679 |
| MW | 132.8 kDa |
| Gene Summary | Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223856 | Phka1 (untagged) - Mouse phosphorylase kinase alpha 1 (Phka1), transcript variant 2, (10ug) |
CNY 15,200.00 |
|
| MG226481 | Phka1 (tGFP-tagged) - Mouse phosphorylase kinase alpha 1 (Phka1) transcript variant 2, (10ug) |
CNY 11,400.00 |
|
| MR226481L3 | Lenti ORF clone of Phka1 (Myc-DDK-tagged) - Mouse phosphorylase kinase alpha 1 (Phka1), transcript variant 2 |
CNY 12,260.00 |
|
| MR226481L4 | Lenti ORF clone of Phka1 (mGFP-tagged) - Mouse phosphorylase kinase alpha 1 (Phka1), transcript variant 2 |
CNY 12,260.00 |
