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Diaph3 (NM_019670) Mouse Untagged Clone
CAT＃: MC223829
Diap3 (untagged) - Mouse diaphanous homolog 3 (Drosophila) (Diap3), (10ug)
CN¥ 15,110.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 4930417P13Rik; Dia2; Diap3; Drf3; mDia2; mKIAA4117; p134MDia2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC223829 representing NM_019670
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAGGCACCGGGCGCGCGCTCTCGGCCGGGACAGCAAGTCGTCGCGGAGGAAGGGCTTGCAGTCCG CGCCGCCCGCTGGCCCCTACGAGCCCGGGGAGAAGCGACCCAAGTTGCATTTAAATATTAGAACACTGAC AGATGATATGCTGGACAAATTTGCCAGTATAAGAATTCCAGGGAGCAAGAAAGAGAGACCTCCCCTTCCC CACCTGAAGACTGTGTCTGGGATCAGTGACAGCTCATCACTGTCCTCAGAGACAATGGAAAACAACCCAA AGGCGCTGCCAGAGAGTGAAGTCTTGAAGCTTTTTGAGAAGATGATGGAAGATATGAATTTAAATGAAGA TAAAAAGGCACCATTGCGGGAAAAAGACTTCGGTATCAAAAAAGAAATGGTGATGCAGTACATTAATACT GCTTCTAAGACAGGAAGTCTTAGAAGTAGCCGACAGATCTCACCTCAGGAATTTCTCCATGAGCTGAAAA TGGGTTACACAGACGAGAGACTTTTCACGTATCTGGAGTCACTCCGAGTATCATTGACCAGTCATCCTGT GAGTTGGGTGCAAAGCTTTGGACACGAGGGACTCGGATTATTGCTGGACATTTTGGAAAAACTAATTAAT GGGCAAATCCAAGAAAAAGTTGTGAAGAAGACTCAGCACAAAGTCATCCAGTGTCTGAGAGCCCTGATGA ACACACAGTATGGCTTAGAAAGGATTATGAGTGACAAGAGGAGTCTTTCCTTGTTGGCAAAAGCCATGGA TCCCAGGCAGCCCGCTATGATGGCAGACGTGGTGAAGCTTCTGTCTGCAGTGTGCATTGTCGGCGAGGAA AGCATCCTTGAAGAAGTGTTAGAAGCCTTGACTTCAGCTGGAGAAGAAAGAAAGATTGACAGATTTTTTT CCATTGTGGAAGGCCTCCGGCATAACTCAGTGAACCTGCAAGTTGCTTGTATGCAGCTCATAAATGCTCT CGTTACATCTCCTGATGATTTGGACTTCAGGCTTCACCTCAGAAATGAATTTATGCGTTGTGGATTGAAA GAGATATTGCCAAACTTAAAGGGCATTAAGAATGATGGCCTGGATATACAACTTAAAGTCTTTGATGAGC ACAAAGAAGAAGATTTGAGTGAGTTTTTCCATCGCCTTGAAGACATTAGAGCTGAACTTGATGAAGCATC TGATGTTTACAGCATGTTATGGGACACAGTTAAGGAAACTCGAGCAGAGGGACATTTTCTTTCTATTCTT CAGCATCTCCTGCTCATTCGCAATGATCGTTTTATAAGAGAGCAGTATTTCAAATTAATTGATGAGTGTG TGTCACAGATTGTATTACATAGAGATGGAACGGACCCTGACTTCACATACAGAAAAAGACTAGATTTGGA CTTAAGTCAGTTTGTAGATGTTTGCATAGATCAGGCCAAACTAGATGAGTGGGAAGAGAAAGCATCCGAA CATTGCAAGAAATTTGAAAAAGAGTGTACTGACCACCAAGAAACCCAGGCTCAATTGCAGAAAAGAGAGG CAAAGATTAATGAGCTTCAAGCAGAGTTACAAGCTTTTAAATCCCAGTTTGGTGCCTTGCCACCTGGTAC AAAAATTCCTTTGCAACCTTCAGTAGAAGGTGAAGCTGGCCCTTCAGCCCTTCCTCCTGCCCCACCAGCA CTCAGTGGAGGAGTGCCGCCTCCCCCACCGCCCCCTCCTCCACCACCCCCACCACTCCCAGGAATGCCAA TGCCATTTGGTGGCCCTGTACCACCACCACCTCCTCTGGGATTCCTGGGTGGGCAAAGCTCTATTCCATT AAACCTGCCATTTGGTTTGAAACCAAAGAAAGAATTTAAGCCTGAAATCAGCATGAGAAGATTGAATTGG TTAAAGATCGGACCAAATGAAATGTCTGAGAACTGCTTCTGGATCAAAGTAAATGAAAATAAGTATGAAA ATAGGGATTTGCTTTGTAAACTTGAGAACACTTTTTGTTGCCAAGAAAAAGAGAAAAGGAATACAAATGA CTTTGATGAGAAGAAAGTTATTAAGAAGAGAATGAAGGAACTTAAATTTCTAGATCCTAAAATTGCTCAG AACCTTTCAATCTTCCTGAGCTCCTTCCGGGTGCCATATGAGAAAATCAGGACGATGATATTGGAAGTGG ATGAAACACAGTTGTCAGAGTCCATGATTCAGAACTTAATAAAGCACCTTCCTGATGAGGAGCAGTTGAA GTCATTGTCCCAGTTTAGAAGTGACTATAACAGTTTGTGTGAGCCTGAGCAGTTCGCTGTTGTGATGAGC AATGTGAAGAGACTCCGGCCACGGCTCAGTGCTATTCTCTTTAAGCTTCAATTTGAAGAGCAGGTGAACA ACATCAAACCTGACATCATGGCTGTCAGTACTGCCTGCGAGGAGATCAAGAAGAGCAAAGGCTTTAGCAA GTTGCTGGAACTTGTGTTGCTAATGGGAAACTACATGAATGCTGGCTCCCGGAATGCTCAAACCTTCGGA TTTGACCTTAGCTCTCTCTGTAAACTGAAGGATACAAAATCTGCAGATCAGAAAACCACACTCCTCCATT TCCTGGTAGATGTATGTGAAGAAAAGCATGCTGACATCCTTCACTTTGTGGACGATTTGGCACATTTAGA CAAAGCTAGCAGAGTCTCTGTAGAAATGCTGGAAAAGAACGTGAAGCAGATGGGAAGGCAGCTTCAACAG CTTGAGAAGAATTTGGAAACCTTTCCCCCTCCTGAGGACTTGCATGACAAGTTTGTGATAAAGATGTCCA GCTTCGTTATCAGTGCGAACGAGCAGTATGAAAAACTCTCCACACTACTGGGCAGCATGACACAATTGTA CCAGAGTATAATGGGCTACTATGCTGTCGACATGAAGAAGGTTTCCGTGGAAGAGTTTTTTAATGATCTG AACAACTTCAGAACTTCATTTATGCTAGCATTAAAGGAAAACATCAAAAAACGAGAAGCAGCAGAAAAGG AGAAACGTGCCAGGATAGCGAAAGAGCGAGCAGAGAAAGAGCGACTTGAACGCCAGCAAGAGAAAAAGCG CTTACTAGAAATGAAAACTGAGGGAGATGAGACAGGAGTGATGGATAGTCTGCTGGAGGCCTTGCAGTCA GGGGCTGCCTTCCGCGACAGAAGAAAAAGGACACCAAAGCTGAAAGATATTCGGCAGAGTCTCAGCCCGA TGTCTCAGAGGCCTGTTCTCAAAGTTTGTAACCATGAAAATCAGAAAATGCAGTTGACAGAAGGGTCACG TCCACACCACAGTATCAATTGCAACTCCACCAGGACTCCAGTCGCCAAGGAGCTTAATTATAATCTAGAC ACTCATGCGTCTACAGGGAGGATCAAGGCAGTTGAGAAGGAAGCCTGTAATGCAGAAAGCAACAAAAAAA AGGAAATGGAACTTCTTGGCTCTGTTGCTAAAAGCGAATCAGTTCCTGAAGTTGAAGCCCTGCTGGCAAG ATTACGAGCTTTATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_019670 |
| Insert Size | 3516 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_019670.1, NP_062644.1 |
| RefSeq Size | 3516 bp |
| RefSeq ORF | 3516 bp |
| Locus ID | 56419 |
| UniProt ID | Q9Z207 |
| Gene Summary | Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers (PubMed:10678165, PubMed:23558171). Required for cytokinesis, stress fiber formation and transcriptional activation of the serum response factor (PubMed:10678165, PubMed:23558171). Binds to GTP-bound form of Rho and to profilin: acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization (PubMed:10678165). DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (PubMed:10678165). Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity (PubMed:23558171).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG218018 | Diap3 (tGFP-tagged) - Mouse diaphanous homolog 3 (Drosophila) (Diap3), (10ug) |
CN¥ 12,830.00 |
|
| MR218018 | Diap3 (Myc-DDK-tagged) - Mouse diaphanous homolog 3 (Drosophila) (Diap3) |
CN¥ 11,690.00 |
|
| MR218018L3 | Lenti ORF clone of Diap3 (Myc-DDK-tagged) - Mouse diaphanous homolog 3 (Drosophila) (Diap3) |
CN¥ 13,590.00 |
|
| MR218018L4 | Lenti ORF clone of Diap3 (mGFP-tagged) - Mouse diaphanous homolog 3 (Drosophila) (Diap3) |
CN¥ 13,590.00 |
