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Diaph3 (NM_019670) Mouse Tagged ORF Clone
CAT＃: MR218018
- TrueORF®
Diap3 (Myc-DDK-tagged) - Mouse diaphanous homolog 3 (Drosophila) (Diap3)
ORF Plasmid: tGFP
"NM_019670" in other vectors (4)
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CNY 11,690.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4930417P13Rik; Dia2; Diap3; Drf3; mDia2; mKIAA4117; p134MDia2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR218018 representing NM_019670
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAGGCACCGGGCGCGCGCTCTCGGCCGGGACAGCAAGTCGTCGCGGAGGAAGGGCTTGCAGTCCG CGCCGCCCGCTGGCCCCTACGAGCCCGGGGAGAAGCGACCCAAGTTGCATTTAAATATTAGAACACTGAC AGATGATATGCTGGACAAATTTGCCAGTATAAGAATTCCAGGGAGCAAGAAAGAGAGACCTCCCCTTCCC CACCTGAAGACTGTGTCTGGGATCAGTGACAGCTCATCACTGTCCTCAGAGACAATGGAAAACAACCCAA AGGCGCTGCCAGAGAGTGAAGTCTTGAAGCTTTTTGAGAAGATGATGGAAGATATGAATTTAAATGAAGA TAAAAAGGCACCATTGCGGGAAAAAGACTTCGGTATCAAAAAAGAAATGGTGATGCAGTACATTAATACT GCTTCTAAGACAGGAAGTCTTAGAAGTAGCCGACAGATCTCACCTCAGGAATTTCTCCATGAGCTGAAAA TGGGTTACACAGACGAGAGACTTTTCACGTATCTGGAGTCACTCCGAGTATCATTGACCAGTCATCCTGT GAGTTGGGTGCAAAGCTTTGGACACGAGGGACTCGGATTATTGCTGGACATTTTGGAAAAACTAATTAAT GGGCAAATCCAAGAAAAAGTTGTGAAGAAGACTCAGCACAAAGTCATCCAGTGTCTGAGAGCCCTGATGA ACACACAGTATGGCTTAGAAAGGATTATGAGTGACAAGAGGAGTCTTTCCTTGTTGGCAAAAGCCATGGA TCCCAGGCAGCCCGCTATGATGGCAGACGTGGTGAAGCTTCTGTCTGCAGTGTGCATTGTCGGCGAGGAA AGCATCCTTGAAGAAGTGTTAGAAGCCTTGACTTCAGCTGGAGAAGAAAGAAAGATTGACAGATTTTTTT CCATTGTGGAAGGCCTCCGGCATAACTCAGTGAACCTGCAAGTTGCTTGTATGCAGCTCATAAATGCTCT CGTTACATCTCCTGATGATTTGGACTTCAGGCTTCACCTCAGAAATGAATTTATGCGTTGTGGATTGAAA GAGATATTGCCAAACTTAAAGGGCATTAAGAATGATGGCCTGGATATACAACTTAAAGTCTTTGATGAGC ACAAAGAAGAAGATTTGAGTGAGTTTTTCCATCGCCTTGAAGACATTAGAGCTGAACTTGATGAAGCATC TGATGTTTACAGCATGTTATGGGACACAGTTAAGGAAACTCGAGCAGAGGGACATTTTCTTTCTATTCTT CAGCATCTCCTGCTCATTCGCAATGATCGTTTTATAAGAGAGCAGTATTTCAAATTAATTGATGAGTGTG TGTCACAGATTGTATTACATAGAGATGGAACGGACCCTGACTTCACATACAGAAAAAGACTAGATTTGGA CTTAAGTCAGTTTGTAGATGTTTGCATAGATCAGGCCAAACTAGATGAGTGGGAAGAGAAAGCATCCGAA CATTGCAAGAAATTTGAAAAAGAGTGTACTGACCACCAAGAAACCCAGGCTCAATTGCAGAAAAGAGAGG CAAAGATTAATGAGCTTCAAGCAGAGTTACAAGCTTTTAAATCCCAGTTTGGTGCCTTGCCACCTGGTAC AAAAATTCCTTTGCAACCTTCAGTAGAAGGTGAAGCTGGCCCTTCAGCCCTTCCTCCTGCCCCACCAGCA CTCAGTGGAGGAGTGCCGCCTCCCCCACCGCCCCCTCCTCCACCACCCCCACCACTCCCAGGAATGCCAA TGCCATTTGGTGGCCCTGTACCACCACCACCTCCTCTGGGATTCCTGGGTGGGCAAAGCTCTATTCCATT AAACCTGCCATTTGGTTTGAAACCAAAGAAAGAATTTAAGCCTGAAATCAGCATGAGAAGATTGAATTGG TTAAAGATCGGACCAAATGAAATGTCTGAGAACTGCTTCTGGATCAAAGTAAATGAAAATAAGTATGAAA ATAGGGATTTGCTTTGTAAACTTGAGAACACTTTTTGTTGCCAAGAAAAAGAGAAAAGGAATACAAATGA CTTTGATGAGAAGAAAGTTATTAAGAAGAGAATGAAGGAACTTAAATTTCTAGATCCTAAAATTGCTCAG AACCTTTCAATCTTCCTGAGCTCCTTCCGGGTGCCATATGAGAAAATCAGGACGATGATATTGGAAGTGG ATGAAACACAGTTGTCAGAGTCCATGATTCAGAACTTAATAAAGCACCTTCCTGATGAGGAGCAGTTGAA GTCATTGTCCCAGTTTAGAAGTGACTATAACAGTTTGTGTGAGCCTGAGCAGTTCGCTGTTGTGATGAGC AATGTGAAGAGACTCCGGCCACGGCTCAGTGCTATTCTCTTTAAGCTTCAATTTGAAGAGCAGGTGAACA ACATCAAACCTGACATCATGGCTGTCAGTACTGCCTGCGAGGAGATCAAGAAGAGCAAAGGCTTTAGCAA GTTGCTGGAACTTGTGTTGCTAATGGGAAACTACATGAATGCTGGCTCCCGGAATGCTCAAACCTTCGGA TTTGACCTTAGCTCTCTCTGTAAACTGAAGGATACAAAATCTGCAGATCAGAAAACCACACTCCTCCATT TCCTGGTAGATGTATGTGAAGAAAAGCATGCTGACATCCTTCACTTTGTGGACGATTTGGCACATTTAGA CAAAGCTAGCAGAGTCTCTGTAGAAATGCTGGAAAAGAACGTGAAGCAGATGGGAAGGCAGCTTCAACAG CTTGAGAAGAATTTGGAAACCTTTCCCCCTCCTGAGGACTTGCATGACAAGTTTGTGATAAAGATGTCCA GCTTCGTTATCAGTGCGAACGAGCAGTATGAAAAACTCTCCACACTACTGGGCAGCATGACACAATTGTA CCAGAGTATAATGGGCTACTATGCTGTCGACATGAAGAAGGTTTCCGTGGAAGAGTTTTTTAATGATCTG AACAACTTCAGAACTTCATTTATGCTAGCATTAAAGGAAAACATCAAAAAACGAGAAGCAGCAGAAAAGG AGAAACGTGCCAGGATAGCGAAAGAGCGAGCAGAGAAAGAGCGACTTGAACGCCAGCAAGAGAAAAAGCG CTTACTAGAAATGAAAACTGAGGGAGATGAGACAGGAGTGATGGATAGTCTGCTGGAGGCCTTGCAGTCA GGGGCTGCCTTCCGCGACAGAAGAAAAAGGACACCAAAGCTGAAAGATATTCGGCAGAGTCTCAGCCCGA TGTCTCAGAGGCCTGTTCTCAAAGTTTGTAACCATGAAAATCAGAAAATGCAGTTGACAGAAGGGTCACG TCCACACCACAGTATCAATTGCAACTCCACCAGGACTCCAGTCGCCAAGGAGCTTAATTATAATCTAGAC ACTCATGCGTCTACAGGGAGGATCAAGGCAGTTGAGAAGGAAGCCTGTAATGCAGAAAGCAACAAAAAAA AGGAAATGGAACTTCTTGGCTCTGTTGCTAAAAGCGAATCAGTTCCTGAAGTTGAAGCCCTGCTGGCAAG ATTACGAGCTTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218018 representing NM_019670
Red=Cloning site Green=Tags(s) MERHRARALGRDSKSSRRKGLQSAPPAGPYEPGEKRPKLHLNIRTLTDDMLDKFASIRIPGSKKERPPLP HLKTVSGISDSSSLSSETMENNPKALPESEVLKLFEKMMEDMNLNEDKKAPLREKDFGIKKEMVMQYINT ASKTGSLRSSRQISPQEFLHELKMGYTDERLFTYLESLRVSLTSHPVSWVQSFGHEGLGLLLDILEKLIN GQIQEKVVKKTQHKVIQCLRALMNTQYGLERIMSDKRSLSLLAKAMDPRQPAMMADVVKLLSAVCIVGEE SILEEVLEALTSAGEERKIDRFFSIVEGLRHNSVNLQVACMQLINALVTSPDDLDFRLHLRNEFMRCGLK EILPNLKGIKNDGLDIQLKVFDEHKEEDLSEFFHRLEDIRAELDEASDVYSMLWDTVKETRAEGHFLSIL QHLLLIRNDRFIREQYFKLIDECVSQIVLHRDGTDPDFTYRKRLDLDLSQFVDVCIDQAKLDEWEEKASE HCKKFEKECTDHQETQAQLQKREAKINELQAELQAFKSQFGALPPGTKIPLQPSVEGEAGPSALPPAPPA LSGGVPPPPPPPPPPPPPLPGMPMPFGGPVPPPPPLGFLGGQSSIPLNLPFGLKPKKEFKPEISMRRLNW LKIGPNEMSENCFWIKVNENKYENRDLLCKLENTFCCQEKEKRNTNDFDEKKVIKKRMKELKFLDPKIAQ NLSIFLSSFRVPYEKIRTMILEVDETQLSESMIQNLIKHLPDEEQLKSLSQFRSDYNSLCEPEQFAVVMS NVKRLRPRLSAILFKLQFEEQVNNIKPDIMAVSTACEEIKKSKGFSKLLELVLLMGNYMNAGSRNAQTFG FDLSSLCKLKDTKSADQKTTLLHFLVDVCEEKHADILHFVDDLAHLDKASRVSVEMLEKNVKQMGRQLQQ LEKNLETFPPPEDLHDKFVIKMSSFVISANEQYEKLSTLLGSMTQLYQSIMGYYAVDMKKVSVEEFFNDL NNFRTSFMLALKENIKKREAAEKEKRARIAKERAEKERLERQQEKKRLLEMKTEGDETGVMDSLLEALQS GAAFRDRRKRTPKLKDIRQSLSPMSQRPVLKVCNHENQKMQLTEGSRPHHSINCNSTRTPVAKELNYNLD THASTGRIKAVEKEACNAESNKKKEMELLGSVAKSESVPEVEALLARLRAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019670 |
| ORF Size | 3513 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019670.2 |
| RefSeq Size | 3516 bp |
| RefSeq ORF | 3516 bp |
| Locus ID | 56419 |
| UniProt ID | Q9Z207 |
| MW | 134.1 kDa |
| Gene Summary | Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers (PubMed:10678165, PubMed:23558171). Required for cytokinesis, stress fiber formation and transcriptional activation of the serum response factor (PubMed:10678165, PubMed:23558171). Binds to GTP-bound form of Rho and to profilin: acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization (PubMed:10678165). DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (PubMed:10678165). Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity (PubMed:23558171).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223829 | Diap3 (untagged) - Mouse diaphanous homolog 3 (Drosophila) (Diap3), (10ug) |
CNY 15,110.00 |
|
| MG218018 | Diap3 (tGFP-tagged) - Mouse diaphanous homolog 3 (Drosophila) (Diap3), (10ug) |
CNY 12,830.00 |
|
| MR218018L3 | Lenti ORF clone of Diap3 (Myc-DDK-tagged) - Mouse diaphanous homolog 3 (Drosophila) (Diap3) |
CNY 13,590.00 |
|
| MR218018L4 | Lenti ORF clone of Diap3 (mGFP-tagged) - Mouse diaphanous homolog 3 (Drosophila) (Diap3) |
CNY 13,590.00 |
