Itgae (NM_172944) Mouse Untagged Clone

CAT#: MC223314

Itgae (untagged) - Mouse integrin alpha E, epithelial-associated (Itgae), transcript variant 2, (10ug)



  "NM_172944" in other vectors (4)

CNY 13,400.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (4)
CD103 Rabbit pAb
    • 100 ul

CNY 2,640.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms A530055J10; alpha-E1; alpha-M290; aM290; CD103
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC223314 representing NM_172944
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGTGGCTCTTCCATACCCTTCTCTGCATGGCCAGCCTGAAGCCCCAGGGAGCCTTCAATTTGGATG
TGGACTGGGCCTGGGTCACAGCACTCCAGCCAGGTGCACCGGCTGTGCTCAGCTCCCTTCTGCATCAAGA
CCCCAGCAACAACCAGACCTGCCTCCTGGTAGCCAGACGCAGCTCCAACAGGAATACAGCCGCCCTGTAT
CGATGCGCTATATCCATCAGTCCAGATGAAATTGCTTGCCAGCCAGTGGAGCACATCTGTATGCCAAAGG
GCAGGTACCAGGGAGTGACATTGGTCGGGAACCACAATGGTGTTTTGGTGTGCATTCAGGTACAGGCCCG
GAAGTTCCGCAGCCTCAACTCAGAGCTCACGGGTGCCTGCAGCCTACTCACCCCCAATTTGGACCTCCAG
GCCCAAGCCTACTTCTCTGACCTCGAAGGGTTCCTGGATCCAGGAGCACATGTGGACTCTGGTGACTACT
GTAGAAGCAAAGGAGGCAGCACAGGGGAGGAGAAGAAGTCAGCCAGGAGACGCCGGACAGTGGAGGAGGA
AGACGAGGAGGAAGATGGCACTGAGATCGCTATTGTCCTGGATGGCTCAGGAAGCATTGAACCCTCAGAC
TTCCAGAAGGCCAAAAATTTCATCTCTACCATGATGAGGAACTTCTATGAGAAGTGTTTTGAGTGCAACT
TTGCCCTGGTGCAGTATGGGGCCGTGATCCAGACTGAGTTTGATCTTCAAGAGAGCCGGGACATTAACGC
CTCCCTTGCCAAAGTTCAGAGCATCGTTCAAGTGAAGGAAGTCACTAAGACCGCCTCAGCCATGCAGCAT
GTCCTAGACAATATCTTCATTCCAAGCCGTGGCTCCAGAAAAAAAGCATTGAAGGTCATGGTGGTACTTA
CTGATGGTGACATATTTGGGGACCCTCTCAATCTTACGACTGTCATCAACTCCCCAAAGATGCAGGGTGT
TGTGCGCTTTGCCATTGGGGTGGGTGATGCATTCAAGAATAACAACACTTACAGAGAACTGAAGCTGATT
GCCTCAGACCCCAAAGAGGCCCACACTTTCAAGGTGACCAACTACTCAGCTCTGGATGGGCTGCTGAGCA
AGCTGCAACAACACATCGTTCATATGGAAGGTACAGTTGGAGATGCCCTTCAATATCAACTCGCACAGAC
TGGTTTCAGTGCCCAGATCCTGGACAAGGGGCAGGTGTTGCTTGGCACTGTGGGGGCCTTTAACTGGTCT
GGGGGTGCGCTGCTCTACAGCACACAGAATGGCCGGGGCTGCTTCCTAAACCAGACAGCCAAGGAGGACT
CCAGGACTGTACAGTACAGCTACCTAGGTTACTCCCTTGCGGTGCTACACAAGGCCCACGGCGTCTCTTA
CGTGGCAGGGGCTCCAAGGCACAAGCTCCGTGGGGCTGTGTTTGAGCTCCGGAAGGAGGACAGAGAGGAG
GATGCCTTTGTGCGACGGATAGAAGGAGAGCAGATGGGGTCCTACTTTGGCTCTGTGCTGTGCCCTGTGG
ATATCGACATGGATGGGACCACAGATTTTCTACTGGTGGCTGCTCCATTTTACCACATCCGTGGAGAAGA
AGGCAGAGTCTATGTGTACCAGGTGCCTGAGCAGGATGCTTCCTTCTCCTTGGCACACACACTGAGTGGG
CACCCTGGGCTCACCAATAGCCGCTTTGGCTTTGCCATGGCAGCTGTGGGGGATATCAACCAAGACAAAT
TCACAGATGTGGCCATTGGGGCCCCTCTGGAGGGTTTTGGGGCAGGTGACGGGGCCAGCTATGGCAGCGT
GTACATCTACAACGGACACTCAGGTGGCTTGTATGACAGTCCTTCCCAGCAGATCCGAGCCTCCTCTGTG
GCCTCGGGACTCCACTACTTCGGCATGTCTGTGTCCGGTGGGTTAGATTTCAATGGCGATGGCCTTGCCG
ATATCACAGTTGGCTCTCGGGACTCGGCGGTTGTGCTCCGCTCACGCCCTGTGGTTGACCTGACCGTATC
TATGACCTTTACCCCCGATGCACTGCCCATGGTCTTCATAGGCAAGATGGATGTAAAACTGTGTTTTGAA
GTTGACTCTTCAGGCGTAGCTTCTGAGCCAGGCCTTAGAGAGATGTTCCTCAACTTTACGGTGGATGTGG
ATGTGACCAAGCAAAGGCAGAGGCTGCAGTGTGAGGACTCGAGCGGTTGTCAGAGCTGCCTCAGGAAGTG
GAACGGAGGGTCCTTTCTGTGTGAACACTTCTGGCTCATCTCCACAGAGGAGCTTTGTGAGGAGGACTGC
TTCTCCAACATCACCATCAAGGTCACCTACGAGTTCCAGACGTCTGGGGGAAGGAGGGACTACCCCAACC
CCACCCTGGACCACTACAAGGAACCCTCTGCCATCTTCCAGCTGCCCTACGAGAAGGACTGCAAGAACAA
AGTGTTCTGCATCGCTGAGATCCAGCTGACCACCAACATCTCCCAGCAGGAACTGGTGGTGGGCGTCACA
AAGGAGGTGACCATGAACATCAGCCTGACTAACTCTGGAGAGGATTCCTACATGACAAACATGGCTCTCA
ATTATCCCAGAAACTTACAGTTTAAGAAGATACAAAAGCCAGTATCTCCAGATGTTCAGTGTGATGACCC
CAAGCCAGTTGCTTCTGTCCTGGTCATGAACTGCAAGATTGGTCACCCCATACTCAAGAGATCATCTGTG
AATGTCTCAGTAACTTGGCAGCTAGAGGAGAGTGTCTTTCCAAATAGGACAGCAGATATCACTGTGACCA
TCTCCAATTCCAATGAGAAGTCTTTGGCCAGAGAGACACGCAGCCTTCAATTCAGACATGCCTTCATTGC
AGTTCTTTCCAGACCCTCAGTGATGTACATGAACACAAGCCAAAGCCCTTCTGACCACAAAGAATTCTTC
TTCAATGTGCATGGGGAGAATCTTTTTGGAGCTGTATTCCAGTTGCAAATTTGTGTCCCCATCAAATTAC
AAGATTTCCAGATTGTAAGAGTGAAGAATCTGACAAAGACTCAGGTATTTAAATGGGAGTCTGTGATTGC
ATCGCTTTCCAAAGACTACAGATTCTCAAAGATATAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_172944
Insert Size 3117 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_172944.2, NP_766532.1
RefSeq Size 3813 bp
RefSeq ORF 3117 bp
Locus ID 16407
Gene Summary Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers. Mice expressing a null mutation of the alpha-E subunit gene exhibit a marked reduction in the numbers of intraepithelial lymphocytes in the gut and in the development of gut-associated lymphoid aggregates, supporting a specific role for this integrin in mediating retention of lymphocytes in the intestinal wall.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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