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Itgae (NM_172944) Mouse Tagged ORF Clone
CAT＃: MR223560
- TrueORF®
Itgae (Myc-DDK-tagged) - Mouse integrin alpha E, epithelial-associated (Itgae), transcript variant 2
ORF Plasmid: tGFP
"NM_172944" in other vectors (4)
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CNY 7,232.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | A530055J10; alpha-E1; alpha-M290; aM290; CD103 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR223560 representing NM_172944
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTGGCTCTTCCATACCCTTCTCTGCATGGCCAGCCTGAAGCCCCAGGGAGCCTTCAATTTGGATG TGGACTGGGCCTGGGTCACAGCACTCCAGCCAGGTGCACCGGCTGTGCTCAGCTCCCTTCTGCATCAAGA CCCCAGCAACAACCAGACCTGCCTCCTGGTAGCCAGACGCAGCTCCAACAGGAATACAGCCGCCCTGTAT CGATGCGCTATATCCATCAGTCCAGATGAAATTGCTTGCCAGCCAGTGGAGCACATCTGTATGCCAAAGG GCAGGTACCAGGGAGTGACATTGGTCGGGAACCACAATGGTGTTTTGGTGTGCATTCAGGTACAGGCCCG GAAGTTCCGCAGCCTCAACTCAGAGCTCACGGGTGCCTGCAGCCTACTCACCCCCAATTTGGACCTCCAG GCCCAAGCCTACTTCTCTGACCTCGAAGGGTTCCTGGATCCAGGAGCACATGTGGACTCTGGTGACTACT GTAGAAGCAAAGGAGGCAGCACAGGGGAGGAGAAGAAGTCAGCCAGGAGACGCCGGACAGTGGAGGAGGA AGACGAGGAGGAAGATGGCACTGAGATCGCTATTGTCCTGGATGGCTCAGGAAGCATTGAACCCTCAGAC TTCCAGAAGGCCAAAAATTTCATCTCTACCATGATGAGGAACTTCTATGAGAAGTGTTTTGAGTGCAACT TTGCCCTGGTGCAGTATGGGGCCGTGATCCAGACTGAGTTTGATCTTCAAGAGAGCCGGGACATTAACGC CTCCCTTGCCAAAGTTCAGAGCATCGTTCAAGTGAAGGAAGTCACTAAGACCGCCTCAGCCATGCAGCAT GTCCTAGACAATATCTTCATTCCAAGCCGTGGCTCCAGAAAAAAAGCATTGAAGGTCATGGTGGTACTTA CTGATGGTGACATATTTGGGGACCCTCTCAATCTTACGACTGTCATCAACTCCCCAAAGATGCAGGGTGT TGTGCGCTTTGCCATTGGGGTGGGTGATGCATTCAAGAATAACAACACTTACAGAGAACTGAAGCTGATT GCCTCAGACCCCAAAGAGGCCCACACTTTCAAGGTGACCAACTACTCAGCTCTGGATGGGCTGCTGAGCA AGCTGCAACAACACATCGTTCATATGGAAGGTACAGTTGGAGATGCCCTTCAATATCAACTCGCACAGAC TGGTTTCAGTGCCCAGATCCTGGACAAGGGGCAGGTGTTGCTTGGCACTGTGGGGGCCTTTAACTGGTCT GGGGGTGCGCTGCTCTACAGCACACAGAATGGCCGGGGCTGCTTCCTAAACCAGACAGCCAAGGAGGACT CCAGGACTGTACAGTACAGCTACCTAGGTTACTCCCTTGCGGTGCTACACAAGGCCCACGGCGTCTCTTA CGTGGCAGGGGCTCCAAGGCACAAGCTCCGTGGGGCTGTGTTTGAGCTCCGGAAGGAGGACAGAGAGGAG GATGCCTTTGTGCGACGGATAGAAGGAGAGCAGATGGGGTCCTACTTTGGCTCTGTGCTGTGCCCTGTGG ATATCGACATGGATGGGACCACAGATTTTCTACTGGTGGCTGCTCCATTTTACCACATCCGTGGAGAAGA AGGCAGAGTCTATGTGTACCAGGTGCCTGAGCAGGATGCTTCCTTCTCCTTGGCACACACACTGAGTGGG CACCCTGGGCTCACCAATAGCCGCTTTGGCTTTGCCATGGCAGCTGTGGGGGATATCAACCAAGACAAAT TCACAGATGTGGCCATTGGGGCCCCTCTGGAGGGTTTTGGGGCAGGTGACGGGGCCAGCTATGGCAGCGT GTACATCTACAACGGACACTCAGGTGGCTTGTATGACAGTCCTTCCCAGCAGATCCGAGCCTCCTCTGTG GCCTCGGGACTCCACTACTTCGGCATGTCTGTGTCCGGTGGGTTAGATTTCAATGGCGATGGCCTTGCCG ATATCACAGTTGGCTCTCGGGACTCGGCGGTTGTGCTCCGCTCACGCCCTGTGGTTGACCTGACCGTATC TATGACCTTTACCCCCGATGCACTGCCCATGGTCTTCATAGGCAAGATGGATGTAAAACTGTGTTTTGAA GTTGACTCTTCAGGCGTAGCTTCTGAGCCAGGCCTTAGAGAGATGTTCCTCAACTTTACGGTGGATGTGG ATGTGACCAAGCAAAGGCAGAGGCTGCAGTGTGAGGACTCGAGCGGTTGTCAGAGCTGCCTCAGGAAGTG GAACGGAGGGTCCTTTCTGTGTGAACACTTCTGGCTCATCTCCACAGAGGAGCTTTGTGAGGAGGACTGC TTCTCCAACATCACCATCAAGGTCACCTACGAGTTCCAGACGTCTGGGGGAAGGAGGGACTACCCCAACC CCACCCTGGACCACTACAAGGAACCCTCTGCCATCTTCCAGCTGCCCTACGAGAAGGACTGCAAGAACAA AGTGTTCTGCATCGCTGAGATCCAGCTGACCACCAACATCTCCCAGCAGGAACTGGTGGTGGGCGTCACA AAGGAGGTGACCATGAACATCAGCCTGACTAACTCTGGAGAGGATTCCTACATGACAAACATGGCTCTCA ATTATCCCAGAAACTTACAGTTTAAGAAGATACAAAAGCCAGTATCTCCAGATGTTCAGTGTGATGACCC CAAGCCAGTTGCTTCTGTCCTGGTCATGAACTGCAAGATTGGTCACCCCATACTCAAGAGATCATCTGTG AATGTCTCAGTAACTTGGCAGCTAGAGGAGAGTGTCTTTCCAAATAGGACAGCAGATATCACTGTGACCA TCTCCAATTCCAATGAGAAGTCTTTGGCCAGAGAGACACGCAGCCTTCAATTCAGACATGCCTTCATTGC AGTTCTTTCCAGACCCTCAGTGATGTACATGAACACAAGCCAAAGCCCTTCTGACCACAAAGAATTCTTC TTCAATGTGCATGGGGAGAATCTTTTTGGAGCTGTATTCCAGTTGCAAATTTGTGTCCCCATCAAATTAC AAGATTTCCAGATTGTAAGAGTGAAGAATCTGACAAAGACTCAGGTATTTAAATGGGAGTCTGTGATTGC ATCGCTTTCCAAAGACTACAGATTCTCAAAGATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223560 representing NM_172944
Red=Cloning site Green=Tags(s) MKWLFHTLLCMASLKPQGAFNLDVDWAWVTALQPGAPAVLSSLLHQDPSNNQTCLLVARRSSNRNTAALY RCAISISPDEIACQPVEHICMPKGRYQGVTLVGNHNGVLVCIQVQARKFRSLNSELTGACSLLTPNLDLQ AQAYFSDLEGFLDPGAHVDSGDYCRSKGGSTGEEKKSARRRRTVEEEDEEEDGTEIAIVLDGSGSIEPSD FQKAKNFISTMMRNFYEKCFECNFALVQYGAVIQTEFDLQESRDINASLAKVQSIVQVKEVTKTASAMQH VLDNIFIPSRGSRKKALKVMVVLTDGDIFGDPLNLTTVINSPKMQGVVRFAIGVGDAFKNNNTYRELKLI ASDPKEAHTFKVTNYSALDGLLSKLQQHIVHMEGTVGDALQYQLAQTGFSAQILDKGQVLLGTVGAFNWS GGALLYSTQNGRGCFLNQTAKEDSRTVQYSYLGYSLAVLHKAHGVSYVAGAPRHKLRGAVFELRKEDREE DAFVRRIEGEQMGSYFGSVLCPVDIDMDGTTDFLLVAAPFYHIRGEEGRVYVYQVPEQDASFSLAHTLSG HPGLTNSRFGFAMAAVGDINQDKFTDVAIGAPLEGFGAGDGASYGSVYIYNGHSGGLYDSPSQQIRASSV ASGLHYFGMSVSGGLDFNGDGLADITVGSRDSAVVLRSRPVVDLTVSMTFTPDALPMVFIGKMDVKLCFE VDSSGVASEPGLREMFLNFTVDVDVTKQRQRLQCEDSSGCQSCLRKWNGGSFLCEHFWLISTEELCEEDC FSNITIKVTYEFQTSGGRRDYPNPTLDHYKEPSAIFQLPYEKDCKNKVFCIAEIQLTTNISQQELVVGVT KEVTMNISLTNSGEDSYMTNMALNYPRNLQFKKIQKPVSPDVQCDDPKPVASVLVMNCKIGHPILKRSSV NVSVTWQLEESVFPNRTADITVTISNSNEKSLARETRSLQFRHAFIAVLSRPSVMYMNTSQSPSDHKEFF FNVHGENLFGAVFQLQICVPIKLQDFQIVRVKNLTKTQVFKWESVIASLSKDYRFSKI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172944 |
| ORF Size | 3114 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_172944.2, NP_766532.1 |
| RefSeq Size | 3813 bp |
| RefSeq ORF | 3117 bp |
| Locus ID | 16407 |
| MW | 115.2 kDa |
| Gene Summary | Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers. Mice expressing a null mutation of the alpha-E subunit gene exhibit a marked reduction in the numbers of intraepithelial lymphocytes in the gut and in the development of gut-associated lymphoid aggregates, supporting a specific role for this integrin in mediating retention of lymphocytes in the intestinal wall.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223314 | Itgae (untagged) - Mouse integrin alpha E, epithelial-associated (Itgae), transcript variant 2, (10ug) |
CNY 13,400.00 |
|
| MG223560 | Itgae (tGFP-tagged) - Mouse integrin alpha E epithelial-associated (Itgae) transcript variant 2, (10ug) |
CNY 9,220.00 |
|
| MR223560L3 | Lenti ORF clone of Itgae (Myc-DDK-tagged) - Mouse integrin alpha E, epithelial-associated (Itgae), transcript variant 2 |
CNY 10,260.00 |
|
| MR223560L4 | Lenti ORF clone of Itgae (mGFP-tagged) - Mouse integrin alpha E, epithelial-associated (Itgae), transcript variant 2 |
CNY 10,260.00 |
