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Flt3 (NM_010229) Mouse Untagged Clone
CAT＃: MC223148
Flt3 (untagged) - Mouse FMS-like tyrosine kinase 3 (Flt3), (10ug)
CNY 6,968.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | B230315G04; CD135; Flk-2; Flk2; Flt-3; Ly72; wmfl |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC223148 representing NM_010229
Red=Cloning site Blue=ORF TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGCGTTGGCGCAGCGCAGCGACCGGCGGCTGCTGCTGCTTGTTGTTTTGTCAGTAATGATTCTTG AGACCGTTACAAACCAAGACCTGCCTGTGATCAAGTGTGTTTTAATCAGTCATGAGAACAATGGCTCATC AGCGGGAAAGCCATCATCGTACCGAATGGTGCGAGGATCCCCAGAAGACCTCCAGTGTGCCCCGAGGCGC CAGAGTGAAGGGACGGTATATGAAGCGGCCACCGTGGAGGTGGCCGAGTCTGGGTCCATCACCCTGCAAG TGCAGCTCGCCACCCCAGGGGACCTTTCCTGCCTCTGGGTCTTTAAGCACAGCTCCCTGGGCTGCCAGCC GCACTTTGATTTACAAAACAGAGGAATCGTTTCCATGGCCATCTTGAACGTGACAGAGACCCAGGCAGGA GAATACCTACTCCATATTCAGAGCGAAGCCGCCAACTACACAGTACTGTTCACAGTGAATGTAAGAGATA CACAGCTGTACGTGCTAAGAAGACCTTACTTTAGGAAGATGGAAAACCAGGACGCACTGCTCTGCATCTC CGAGGGTGTTCCAGAGCCCACTGTGGAGTGGGTGCTCTGCAGCTCCCACAGGGAAAGCTGTAAAGAAGAA GGCCCTGCTGTTGTCAGAAAGGAGGAAAAGGTACTTCATGAGTTGTTCGGAACAGACATCAGATGCTGTG CTAGAAATGCACTGGGCCGCGAATGCACCAAGCTGTTCACCATAGATCTAAACCAGGCTCCTCAGAGCAC ACTGCCCCAGTTATTCCTGAAAGTGGGGGAACCCTTGTGGATCAGGTGTAAGGCCATCCATGTGAACCAT GGATTCGGGCTCACCTGGGAGCTGGAAGACAAAGCCCTGGAGGAGGGCAGCTACTTTGAGATGAGTACCT ACTCCACAAACAGGACCATGATTCGGATTCTCTTGGCCTTTGTGTCTTCCGTGGGAAGGAACGACACCGG ATATTACACCTGCTCTTCCTCAAAGCACCCCAGCCAGTCAGCGTTGGTGACCATCCTAGAAAAAGGGTTT ATAAACGCTACCAGCTCGCAAGAAGAGTATGAAATTGACCCGTACGAAAAGTTCTGCTTCTCAGTCAGGT TTAAAGCGTACCCACGAATCCGATGCACGTGGATCTTCTCTCAAGCCTCATTTCCTTGTGAACAGAGAGG CCTGGAGGATGGGTACAGCATATCTAAATTTTGCGATCATAAGAACAAGCCAGGAGAGTACATATTCTAT GCAGAAAATGATGACGCCCAGTTCACCAAAATGTTCACGCTGAATATAAGAAAGAAACCTCAAGTGCTAG CAAATGCCTCAGCCAGCCAGGCGTCCTGTTCCTCTGATGGCTACCCGCTACCCTCTTGGACCTGGAAGAA GTGTTCGGACAAATCTCCCAATTGCACGGAGGAAATCCCAGAAGGAGTTTGGAATAAAAAGGCTAACAGA AAAGTGTTTGGCCAGTGGGTGTCGAGCAGTACTCTAAATATGAGTGAGGCCGGGAAAGGGCTTCTGGTCA AATGCTGTGCGTACAATTCTATGGGCACGTCTTGCGAAACCATCTTTTTAAACTCACCAGGCCCCTTCCC TTTCATCCAAGACAACATCTCCTTCTATGCGACCATTGGGCTCTGTCTCCCCTTCATTGTTGTTCTCATT GTGTTGATCTGCCACAAATACAAAAAGCAATTTAGGTACGAGAGTCAGCTGCAGATGATCCAGGTGACTG GCCCCCTGGATAACGAGTACTTCTACGTTGACTTCAGGGACTATGAATATGACCTTAAGTGGGAGTTCCC GAGAGAGAACTTAGAGTTTGGGAAGGTCCTGGGGTCTGGCGCTTTCGGGAGGGTGATGAACGCCACGGCC TATGGCATTAGTAAAACGGGAGTCTCAATTCAGGTGGCGGTGAAGATGCTAAAAGAGAAAGCTGACAGCT GTGAAAAAGAAGCTCTCATGTCGGAGCTCAAAATGATGACCCACCTGGGACACCATGACAACATCGTGAA TCTGCTGGGGGCATGCACACTGTCAGGGCCAGTGTACTTGATTTTTGAATATTGTTGCTATGGTGACCTC CTCAACTACCTAAGAAGTAAAAGAGAGAAGTTTCACAGGACATGGACAGAGATTTTTAAGGAACATAATT TCAGTTTTTACCCTACTTTCCAGGCACATTCAAATTCCAGCATGCCTGGTTCACGAGAAGTTCAGTTACA CCCGCCCTTGGATCAGCTCTCAGGGTTCAATGGGAATTTAATTCATTCTGAAGATGAGATTGAATATGAA AACCAGAAGAGGCTGGCAGAAGAAGAGGAGGAAGATTTGAACGTGCTGACGTTTGAAGACCTCCTTTGCT TTGCGTACCAAGTGGCCAAAGGCATGGAATTCCTGGAGTTCAAGTCGTGTGTCCACAGAGACCTGGCAGC CAGGAATGTGTTGGTCACCCACGGGAAGGTGGTGAAGATCTGTGACTTTGGACTGGCCCGAGACATCCTG AGCGACTCCAGCTACGTCGTCAGGGGCAACGCACGGCTGCCGGTGAAGTGGATGGCACCTGAGAGCTTAT TTGAAGGGATCTACACAATCAAGAGTGACGTCTGGTCCTACGGCATCCTTCTCTGGGAGATATTTTCACT GGGTGTGAACCCTTACCCTGGCATTCCTGTCGACGCTAACTTCTATAAACTGATTCAGAGTGGATTTAAA ATGGAGCAGCCATTCTATGCCACAGAAGGGATATACTTTGTAATGCAATCCTGCTGGGCTTTTGACTCAA GGAAGCGGCCATCCTTCCCCAACCTGACTTCATTTTTAGGATGTCAGCTGGCAGAGGCAGAAGAAGCGAT GTATCAGAACATGGGTGGCAACGTCCCAGAACATCCATCCATCTACCAAAACAGGCGGCCCCTCAGCAGA GAGGCAGGCTCAGAGCCGCCATCGCCACAGGCCCAGGTGAAGATTCACGGAGAAAGAAGTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_010229 |
| Insert Size | 3003 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_010229.2, NP_034359.2 |
| RefSeq Size | 3664 bp |
| RefSeq ORF | 3003 bp |
| Locus ID | 14255 |
| UniProt ID | Q00342 |
| Gene Summary | Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG223373 | Flt3 (tGFP-tagged) - Mouse FMS-like tyrosine kinase 3 (Flt3), (10ug) |
CNY 12,584.00 |
|
| MR223373 | Flt3 (Myc-DDK-tagged) - Mouse FMS-like tyrosine kinase 3 (Flt3) |
CNY 10,984.00 |
|
| MR223373L3 | Lenti ORF clone of Flt3 (Myc-DDK-tagged) - Mouse FMS-like tyrosine kinase 3 (Flt3) |
CNY 11,880.00 |
|
| MR223373L4 | Lenti ORF clone of Flt3 (mGFP-tagged) - Mouse FMS-like tyrosine kinase 3 (Flt3) |
CNY 11,880.00 |
