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Top3a (NM_009410) Mouse Untagged Clone
CAT＃: MC223078
Top3a (untagged) - Mouse topoisomerase (DNA) III alpha (Top3a), (10ug)
CNY 12,920.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | MGC106383 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC223078 representing NM_009410
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCTTCCCGGTCACCCTCTTAGCGTTTCAGTGGCACCGACGGCCTGGAGGCCGTGCCCTGTCCCGCG CTGCCATGGAAGTGGCCTTCCGAGGAGTGCGAAAAGTTCTCTGCGTGGCGGAGAAAAACGACGCTGCCAA GGGGATCGCCGACTTGCTGTCCAACGGGCGTATGAGGCGGAAAGAAGGCCTTTCTAAATTCAACAAGATT TATGAATTTGACTATCATCTGTATGGCCAGAATGTTACTATGATAATGACTTCAGTCTCTGGACATTTGC TGGCTCATGACTTCCAGATGCAGTTTCGGAAATGGCAGAGCTGCAATCCCCTTGTCCTCTTTGAAGCAGA AATTGAAAAGTATTGCCCAGAAAATTTTATAGACATCAAGAAAACTCTAGAACGAGAGACACATCATTGT CAGGCCCTGGTGATCTGGACGGACTGTGATAGGGAAGGTGAGAACATCGGCTTTGAGATTATCCACGTAT GCAAGGCTGTAAAACCCAATCTACGGGTGCTGAGAGCCCGCTTCTCTGAGATCACGCCACACGCCGTCAG AACAGCCTGTGAAAACCTAACTGAGCCTGACCAGAGAGTGAGTGACGCAGTGGATGTGAGGCAAGAGCTG GACCTGAGGATCGGAGCTGCCTTCACAAGGTTCCAGACCCTGCGGCTCCAGAGGATCTTCCCTGAGGTGC TGGCAGAGCAGCTCATCAGCTACGGCAGTTGCCAGTTCCCAACACTGGGGTTTGTGGTGGAGAGGTTCAA GGCCATTCAGGCTTTTGTGCCAGAAGTCTTCCACAAAATTAAAGTAACTCATGACCACAAAGATGGGACC GTAGAGTTCAACTGGAAACGATACCGTCTCTTTAACCACACAGCTTGTCTTGTCCTTTATCAGTTGTGCA TGGAGGATCCCATGGCGACTGTGGTAGAGGTCAGATCTAAGCCAAAGAGCAAGTGGAGGCCGCAGGCTTT GGACACGGTGGAGCTAGAGAAACTGGCATCTCGGAAATTGAGAATAAATGCCAAAGAAACCATGAGGATC GCGGAAAAGCTCTATACACAAGGGTACATCAGCTACCCCCGGACAGAAACAAACATCTTCCCCAAAGACT TAAACCTGGTTGCATTGGTAGAACAGCAGACCGTGGATCCACACTGGGGGGCTTTTGCTCAGACCATTCT AGAGCGAGGTGGCCCTACTCCCCGAAACGGGAGCAAGTCTGATCAAGCTCACCCTCCCATTCACCCCACC AAATACACCAGCGGCTTGCAGGGAGATGATCGGCGACTGTACGAGTTCATTGTTCGCCATTTCCTGGCTT GCTGCTCTCAGGACGCTCAGGGGCAAGAGACTACCGTAGAGATCGACATTGCCCAAGAGCGCTTTGTGGC CCACGGGCTCATCATTCTGGCCCGTAACTACCTAGATGTGTATCCGTATGATCACTGGAGTGACAAGCTC CTCCCCGTCTATGAACAAGGCTCCCACTTCCAGCCCAGCACTGTGGAAATGGTGGATGGAGAGACTAGCC CACCCCAACTGCTTACCGAAGCTGACCTCATCGCCCTCATGGAGAAACATGGGATCGGTACTGATGCAAC TCATGCAGAACACATTGAGACCATCAAAGCCCGGATGTATGTGGGACTCACCTCAGACAAGCGGTTCCTG CCGGGGCACCTGGGCATGGGACTTGTAGAAGGTTACGATTCCATGGGTTATGAGATGTCCAAGCCTGACC TCCGTGCCGAGCTGGAAGCTGATCTCAAGCTGATCTGTGAGGGCAAGAAGGATAAGTTTCAAGTTCTAAG GCAGCAAGTGCAAAAATACAAGCAGGTTTTCATTGAAGCAGTGGCGAAGGCAAAGAAGTTGGATGAGGCT TTATCTCAGTACTTAGGAGAAAGAACAGAGATGGCCCAGCAAGAAGAGATCTACCCAGCCATGCCAGAGC CTGTTCGGAAGTGCCCACAGTGTAACAAGGATATGGTCCTCAAGACCAAGAAGAGTGGTGGGTTCTACCT CAGCTGCATGGGGTTCCCAGAGTGTCGGTCAGCTGTGTGGTTTCCTGACTCGGTGCTGGAGGCCAGCAGG GACAACAGTGTGTGTTCTGTTTGTCAGCCACCCCCTGTGTACAGGTTGAAGTTGAAGTTTAAGCGTGGTA GCCTTCCCCCAGCTATGCCCCTGGAGTTTGTCGGCTGCATAGGTGGATGTGATGAGACTTTGAAGGAAAT CTTCGGCCTGCGATTTCCACGGGCTCTCCCAAGAGCTAGCCAGCCCTCTGGCCACCTGCAGGCCAGCCAG GCCCTCAACAGGATGGACAGCAGTCAGCATAACCTGTCTCAACCTCTGGTTAACAGACATACTAGACCTT CAAAGACAGTGGCCCAAGCCTTGCTACCACCCACTACTGCTGGTGAAAGCAACTCTGTGACTTGCAATTG TGGCCGGGAGGCTGTGCTGCTCACTGTCCGCAAGCAGGGCCCCAACCAGGGCCGGCATTTCTACAAGTGC AGTAATGGTGACTGCAACTTCTTCCTGTGGGCTGACAGCAGCCATTCCACTGGAGGAGGGACCCCCACCT CTGCATCAGGACCCCCAGGCAGCTCTGTAGGATGCCCATCCAGTGTAGGCAGCCACATGGATGGGTTTGG CAGCCTTGGCAGCGACAGTGATGGAGGTACACCCTGCCTGTGCGGGCAGCCTGCTGTCACACGGACTGTT CAGAAGGATGGACCCAACAAAGGACGCCAGTTCCACACCTGTGCCAAGCCACGAGAGCAGCAGTGTGGCT TCTTTCAGTGGGTGGATGAGAATGTGGCCCCAGGAAGTTTTGCAGCCCCTGCATGGCCAGGAGGCAGAGG AAAAGCCCAGAGACCAGAGGCTGCAAGCAAAAGACCAAGAGCTGGTTCCTCAGATGCAGGGTCCACAGTA AAAAAGCCTCGGAAATGTAGCCTTTGTCACCAGCCTGGACACACCCGAACCTTTTGTCCTCAGAACAGAT GA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_009410 |
| Insert Size | 3012 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_009410.2, NP_033436.1 |
| RefSeq Size | 3740 bp |
| RefSeq ORF | 3012 bp |
| Locus ID | 21975 |
| UniProt ID | O70157 |
| Gene Summary | Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. As an essential component of the RMI complex it is involved in chromosome separation and the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity. It is required for mtDNA decatenation and segregation after completion of replication, in a process that does not require BLM, RMI1 and RMI2.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211428 | Top3a (tGFP-tagged) - Mouse topoisomerase (DNA) III alpha (Top3a) |
CNY 9,220.00 |
|
| MR211428 | Top3a (Myc-DDK-tagged) - Mouse topoisomerase (DNA) III alpha (Top3a) |
CNY 6,984.00 |
|
| MR211428L3 | Lenti ORF clone of Top3a (Myc-DDK-tagged) - Mouse topoisomerase (DNA) III alpha (Top3a) |
CNY 10,260.00 |
|
| MR211428L4 | Lenti ORF clone of Top3a (mGFP-tagged) - Mouse topoisomerase (DNA) III alpha (Top3a) |
CNY 10,260.00 |
