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Opa1 (NM_133752) Mouse Untagged Clone
CAT＃: MC222838
Opa1 (untagged) - Mouse optic atrophy 1 homolog (human) (Opa1), nuclear gene encoding mitochondrial protein, transcript variant 2, (10ug)
CNY 12,260.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1200011N24Rik; AI225888; AI847218; lilr3; mKIAA0567 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222838 representing NM_133752
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGCGAGCAGGTCGGGCGGCCGTGGCCTGTGAAGTCTGCCAATCCTTAGTGAAACACAGTTCTGGAA TACAAAGAAACGTACCGCTCCAAAAACTCCATCTGGTTTCACGAAGTATTTATCGTTCACATCATCCTGC CCTCAAGCTTCAAAGACCCCAACTAAGGACACCATTTCAGCAGTTCTCTTCTCTAACTCACCTTTCATTA CATAAATTGAAACTTTCTCCAATTAAATATGGCTACCAGCCCCGCAGGAACTTTTGGCCAGCAAGGTTAG CTGCAAGACTCTTAAAACTTCGATATATCATACTGGGATCTGCTGTTGGAGGTGGCTATACAGCCAAAAA AACCTTCGATGAATGGAAAGATATGATACCAGACCTTAGTGACTATAAGTGGATTGTGCCTGACTTTATA TGGGAAATTGATGAGTATATTGATTTGGAGAAAATTAGAAAAGCCCTGCCCAGCTCAGAAGACCTTGCCA GTTTAGCTCCCGACCTGGACAAGATTACTGAGAGCCTCAGCTTGTTGAAGGACTTCTTCACTGCAGGTTC ACCTGGAGAAACAGCATTTCGAGCAACAGATCATGGATCTGAAAGTGACAAGCATTACAGGAAGGTGTCA GACAAAGAAAAGATTGACCAACTTCAAGAAGAACTTCTGCATACTCAGTTAAAGTATCAGAGGATCTTGG AGCGCCTGGAAAAGGAGAACAAAGAGCTGCGGAAGCTGGTGCTGCAGAAGGACGACAAAGGCATCCACCA CAGGAAGCTCAAGAAATCTTTGATTGATATGTATTCTGAAGTTCTTGATGTTCTTTCTGATTATGATGCC AGTTACAATACACAAGATCACCTACCACGGGTTGTTGTGGTTGGAGATCAGAGTGCTGGGAAAACCAGTG TGCTGGAAATGATTGCTCAGGCCCGGATCTTCCCGAGAGGGTCCGGCGAGATGATGACACGCTCTCCAGT GAAGGTGACTCTCAGTGAAGGCCCTCACCATGTGGCCTTGTTTAAAGATAGCTCTCGGGAATTTGATCTC ACCAAGGAGGAAGATCTTGCAGCATTAAGACATGAAATCGAACTCCGAATGAGGAAAAATGTGAAAGAAG GTTGTACTGTTAGCCCCGAGACCATATCTCTAAATGTCAAAGGCCCTGGGCTGCAGAGGATGGTGCTCGT GGACTTGCCTGGTGTCATCAACACCGTGACATCAGGCATGGCTCCCGACACAAAGGAAACTATTTTCAGT ATCAGCAAAGCTTACATGCAGAATCCTAACGCCATCATCCTGTGCATCCAAGACGGATCCGTAGATGCTG AGCGCAGTATTGTTACAGACTTGGTCAGTCAAATGGATCCTCATGGAAGAAGAACCATATTTGTTTTGAC CAAAGTAGACCTGGCAGAAAAAAATGTAGCCAGTCCAAGCAGGATACAACAGATAATTGAAGGCAAGCTC TTCCCAATGAAAGCTCTGGGTTATTTTGCTGTCGTAACAGGAAAAGGAAACAGCTCTGAAAGTATTGAAG CTATAAGAGAGTATGAAGAGGAATTTTTTCAGAATTCAAAACTGCTAAAGACAAGCATGCTAAAGGCACA CCAGGTCACCACGAGAAATCTCAGCCTTGCTGTGTCAGACTGCTTTTGGAAAATGGTTCGAGAGTCAGTT GAACAACAGGCTGATAGTTTTAAAGCCACGCGCTTTAACCTAGAGACGGAATGGAAGAATAACTACCCGC GCCTGCGAGAGCTCGACAGGAATGAACTCTTTGAAAAAGCTAAAAATGAGATCCTCGATGAGGTCATCAG TCTGAGCCAGGTCACGCCAAAGCACTGGGAGGAAATCCTGCAGCAATCCCTGTGGGAACGAGTGTCAACA CATGTGATTGAGAACATCTACCTTCCAGCTGCCCAGACCATGAATTCGGGAACATTTAACACCACAGTAG ACATCAAGCTTAAACAGTGGACTGACAAGCAGCTTCCTAATAAAGCAGTCGAGGTTGCCTGGGAGACTCT ACAAGAGGAATTTTCCCGCTTCATGACAGAACCCAAAGGAAAGGAACACGACGACATATTTGACAAACTT AAGGAGGCTGTGAAGGAGGAGAGTATCAAGCGGCACAAGTGGAACGACTTTGCCGAGGATAGCTTGAGGG TTATTCAGCACAATGCTTTGGAAGACCGGTCCATATCAGATAAGCAACAGTGGGATGCAGCCATTTACTT CATGGAAGAGGCGCTTCAAGGTCGTCTCAAGGATACTGAAAATGCTATTGAAAACATGATTGGGCCAGAC TGGAAAAAGAGGTGGATGTACTGGAAGAATCGGACCCAAGAGCAGTGTGTTCACAACGAAACCAAGAACG AGTTGGAGAAGATGCTGAAGGTTAATGATGAGCACCCAGCTTACCTGGCAAGTGATGAGATTACCACAGT CCGGAAGAACCTGGAGTCTCGAGGAGTGGAAGTCGATCCAAGCTTGATTAAGGATACTTGGCATCAAGTT TATAGAAGACATTTCTTAAAAACAGCTCTAAATCATTGTAACCTTTGTCGCAGAGGTTTTTATTACTACC AGAGGCATTTTATAGATTCTGAGCTGGAATGCAATGACGTGGTCCTGTTTTGGCGAATACAGCGCATGCT CGCTATCACTGCCAATACATTAAGGCAGCAGCTTACAAACACTGAAGTTAGGCGACTAGAGAAAAACGTT AAAGAGGTATTAGAAGATTTTGCAGAAGACGGTGAGAAGAAGGTTAAATTGCTCACTGGCAAACGAGTTC AGCTGGCAGAAGATCTCAAGAAAGTTAGAGAAATTCAAGAAAAGCTTGATGCTTTCATTGAAGCTCTTCA CCAGGAGAAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_133752 |
| Insert Size | 2883 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_133752.3, NP_598513.1 |
| RefSeq Size | 5948 bp |
| RefSeq ORF | 2883 bp |
| Locus ID | 74143 |
| UniProt ID | P58281 |
| Gene Summary | Dynamin-related GTPase that is essential for normal mitochondrial morphology by regulating the equilibrium between mitochondrial fusion and mitochondrial fission (PubMed:11847212, PubMed:24616225, PubMed:26785494, PubMed:28746876). Coexpression of isoform 1 with shorter alternative products is required for optimal activity in promoting mitochondrial fusion (By similarity). Binds lipid membranes enriched in negatively charged phospholipids, such as cardiolipin, and promotes membrane tubulation. The intrinsic GTPase activity is low, and is strongly increased by interaction with lipid membranes (By similarity). Plays a role in remodeling cristae and the release of cytochrome c during apoptosis (PubMed:16839884, PubMed:16839885). Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space (PubMed:16839884, PubMed:16839885). Plays a role in mitochondrial genome maintenance (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an in-frame exon in the 5' coding region, compared to variant 1. The resulting protein (isoform 2) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG225663 | Opa1 (tGFP-tagged) - Mouse optic atrophy 1 homolog (human) (Opa1) nuclear gene encoding mitochondrial protein, (10ug) |
CNY 7,890.00 |
|
| MR225663 | Opa1 (Myc-DDK-tagged) - Mouse optic atrophy 1 homolog (human) (Opa1), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 7,040.00 |
|
| MR225663L3 | Lenti ORF clone of Opa1 (Myc-DDK-tagged) - Mouse optic atrophy 1 homolog (human) (Opa1), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 9,030.00 |
|
| MR225663L4 | Lenti ORF clone of Opa1 (mGFP-tagged) - Mouse optic atrophy 1 homolog (human) (Opa1), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 9,030.00 |
