Opa1 (NM_133752) Mouse Tagged Lenti ORF Clone
CAT#: MR225663L4
- LentiORF®
Lenti ORF clone of Opa1 (mGFP-tagged) - Mouse optic atrophy 1 homolog (human) (Opa1), nuclear gene encoding mitochondrial protein, transcript variant 2
"NM_133752" in other vectors (4)
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CNY 9,030.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | mGFP |
Synonyms | 1200011N24Rik; AI225888; AI847218; lilr3; mKIAA0567 |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(MR225663).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_133752 |
ORF Size | 2883 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_133752.2 |
RefSeq Size | 5948 bp |
RefSeq ORF | 2883 bp |
Locus ID | 74143 |
UniProt ID | P58281 |
Gene Summary | Dynamin-related GTPase that is essential for normal mitochondrial morphology by regulating the equilibrium between mitochondrial fusion and mitochondrial fission (PubMed:11847212, PubMed:24616225, PubMed:26785494, PubMed:28746876). Coexpression of isoform 1 with shorter alternative products is required for optimal activity in promoting mitochondrial fusion (By similarity). Binds lipid membranes enriched in negatively charged phospholipids, such as cardiolipin, and promotes membrane tubulation. The intrinsic GTPase activity is low, and is strongly increased by interaction with lipid membranes (By similarity). Plays a role in remodeling cristae and the release of cytochrome c during apoptosis (PubMed:16839884, PubMed:16839885). Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space (PubMed:16839884, PubMed:16839885). Plays a role in mitochondrial genome maintenance (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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FAQs |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC222838 | Opa1 (untagged) - Mouse optic atrophy 1 homolog (human) (Opa1), nuclear gene encoding mitochondrial protein, transcript variant 2, (10ug) |
CNY 12,260.00 |
|
MG225663 | Opa1 (tGFP-tagged) - Mouse optic atrophy 1 homolog (human) (Opa1) nuclear gene encoding mitochondrial protein, (10ug) |
CNY 7,890.00 |
|
MR225663 | Opa1 (Myc-DDK-tagged) - Mouse optic atrophy 1 homolog (human) (Opa1), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 7,040.00 |
|
MR225663L3 | Lenti ORF clone of Opa1 (Myc-DDK-tagged) - Mouse optic atrophy 1 homolog (human) (Opa1), nuclear gene encoding mitochondrial protein, transcript variant 2 |
CNY 9,030.00 |