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Copb1 (NM_033370) Mouse Untagged Clone
CAT＃: MC222788
Copb1 (untagged) - Mouse coatomer protein complex, subunit beta 1 (Copb1), (10ug)
CNY 12,160.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2610019B04Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222788 representing NM_033370
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGCAGCCGAGAACGTGTGCTATACGTTAATTAACGTGCCAATGGATTCAGAACCCCCTTCGGAAA TCAGCTTAAAAAATGACCTGGAGAAAGGCGATGTGAAGTCAAAGACTGAAGCTTTGAAGAAAGTGATCAT TATGATTCTGAATGGGGAAAAGCTTCCCGGACTCCTGATGACAATCATTCGCTTCGTGCTGCCTCTTCAG GATCACACCATCAAAAAGTTGCTCCTGGTCTTCTGGGAGATTGTTCCTAAAACAACTCCAGATGGAAGGC TCTTACATGAAATGATTCTTGTGTGTGATGCATACAGAAAGGATCTCCAGCACCCCAATGAGTTTATTCG TGGATCTACTCTTCGTTTCCTTTGCAAATTGAAGGAAGCAGAATTGCTGGAACCTTTAATGCCTGCTATC CGTGCGTGCTTGGAACATCGTCACAGCTATGTTAGGAGAAATGCTGTTTTGGCCATCTATACCATCTACA GAAATTTTGAACATCTTATACCTGATGCTCCTGAACTGATACATGATTTTCTTGTGAATGAGAAGGATGC AAGCTGCAAAAGAAATGCATTTATGATGCTAATTCACGCAGATCAGGATCGAGCTTTGGATTATTTAAGT ACATGTATTGATCAAGTTCAGACATTTGGAGACATTCTACAGTTGGTTATTGTTGAACTAATTTATAAGG TCTGTCATGCTAATCCATCAGAAAGGGCCCGCTTCATTCGCTGTATCTATAACTTACTGCAGTCATCTAG TCCTGCTGTAAAATATGAGGCTGCTGGGACACTGGTAACACTGTCAAGTGCACCAACTGCAATAAAGGCT GCTGCTCAGTGTTACATTGATTTAATTATTAAGGAGAGTGATAACAATGTAAAGCTCATCGTCCTGGACC GCTTGGTGGAATTAAAAGAGCATCCTGCTCATGAACGAGTCCTGCAGGATCTGGTCATGGACATCCTAAG AGTACTGAGTACGCCAGACTTAGAAGTGCGTAAGAAAACGCTGCAGTTAGCACTGGATCTTGTCTCATCT AGGAATGTTGAAGAGTTGGTTATTGTCTTAAAGAAGGAAGTAATTAAAACAAATAATGTGTCTGAGCATG AAGACACTGACAAATACCGACAGCTTCTCGTGCGAACACTGCATTCCTGCTCTGTCCGATTTCCAGATAT GGCTGCAAATGTTATTCCTGTGTTAATGGAATTTCTCAGTGACAGTAATGAAGCAGCAGCTGCTGATGTC TTAGAGTTTGTTCGTGAAGCCATTCAGCGCTTTGACAACCTGAGAATGCTGATTGTTGAGAAGATGCTGG AAGTCTTCCATGCTATTAAGTCTGTCAAGATTTACCGAGGAGCATTGTGGATCCTGGGAGAGTACTGCAG TACAAAGGAAGACATTCAGAGTGTGATGACTGAGGTCCGCAGGTCCCTTGGGGAGATACCAATTGTAGAA TCAGAAATAAAGAAAGAAGCTGGCGAGTTAAAACCAGAAGAAGAAATAACTGTTGGGCCCGTTCAGAAAT TGGTTACGGAGATGGGCACCTATGCTACTCAGAGTGCGCTCAGCAGTTCCAGACCTACCAAGAAAGAAGA AGACAGACCACCCTTGAGAGGGTTCCTCCTGGATGGAGATTTCTTTGTTGCTGCTTCCCTCGCCACAACT CTAACCAAGATTGCATTGCGCTATGTAGCGTTGGTTCAGGAGAAGAAAAAGCAAAATTCTTTTGTTGCTG AGGCTATGTTGCTTATGGCAACTATCCTTCATTTGGGAAAATCCTCTCTTCCTAAGAAGCCAATTACTGA TGATGATGTAGATCGAATTTCCCTGTGCCTCAAGGTCTTATCTGAATGCTCACCTTTAATGAATGACATC TTTAATAAGGAGTGCAGACAGTCTCTTTCCCAAATGTTATCTGCCAAACTCGAAGAAGAGAAACTATCCC AGAAGAAAGAATCTGAAAAGAGAAATGTGACAGTACAGCCTGATGACCCCATTTCCTTCATGCAACTAAC TGCGAAGAATGAAATGAACTGCAAGGAAGATCAGTTCCAGCTGAGTTTGTTGGCAGCAATGGGTAATACT CAGAGGAAAGAGGCAGCTGATCCCCTAGCATCCAAACTTAACAAGGTCACTCAGTTGACAGGCTTCTCGG ATCCAGTGTATGCAGAAGCCTATGTTCATGTCAATCAGTACGACATCGTCCTGGATGTTCTGGTTGTAAA CCAAACCAGTGATACTTTGCAGAACTGCACATTAGAGTTAGCCACTCTAGGGGATCTGAAACTTGTGGAA AAACCATCTCCTTTGACTCTTGCTCCTCATGACTTTGCAAATATTAAAGCTAATGTCAAGGTAGCATCAA CAGAAAACGGAATAATTTTTGGCAATATAGTTTATGATGTTTCTGGAGCAGCAAGTGACAGAAACTGTGT GGTCCTCAGTGACATCCACATTGACATCATGGACTATATCCAGCCTGCAACTTGCACTGATGCTGAGTTC CGTCAGATGTGGGCCGAATTTGAATGGGAAAATAAAGTGACAGTTAACACCAACATGACTGACCTGAATG ACTACTTACAGCACATCCTCAAGTCGACCAACATGAAGTGCCTGACTCCAGAGAAGGCCCTTTCTGGATA CTGTGGCTTTATGGCAGCCAATCTTTATGCACGTTCTATATTTGGAGAGGATGCACTTGCAAATGTCAGC ATTGAGAAGCCAGTTCACCAGGGACCAGATGCTGCCGTTACTGGCCATATAAGAATTCGTGCAAAAAGTC AGGGAATGGCCTTGAGCCTTGGAGATAAAATCAACCTGTCTCAAAAGAAGACTAGTCTATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_033370 |
| Insert Size | 2862 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_033370.3, NP_203534.1 |
| RefSeq Size | 3315 bp |
| RefSeq ORF | 2862 bp |
| Locus ID | 70349 |
| UniProt ID | Q9JIF7 |
| Gene Summary | The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Involved in the Golgi disassembly and reassembly processes during cell cycle. Involved in autophagy by playing a role in early endosome function. Plays a role in organellar compartmentalization of secretory compartments including endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC), Golgi, trans-Golgi network (TGN) and recycling endosomes, and in biosynthetic transport of CAV1 (By similarity). Plays a functional role in facilitating the transport of kappa-type opioid receptor mRNAs into axons and enhances translation of these proteins in cortical neurons. Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte triglyceride lipase (PNPLA2) with the lipid droplet surface to mediate lipolysis.[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211294 | Copb1 (tGFP-tagged) - Mouse coatomer protein complex, subunit beta 1 (Copb1) |
CNY 7,890.00 |
|
| MR211294 | Copb1 (Myc-DDK-tagged) - Mouse coatomer protein complex, subunit beta 1 (Copb1) |
CNY 6,976.00 |
|
| MR211294L3 | Lenti ORF clone of Copb1 (Myc-DDK-tagged) - Mouse coatomer protein complex, subunit beta 1 (Copb1) |
CNY 9,030.00 |
|
| MR211294L4 | Lenti ORF clone of Copb1 (mGFP-tagged) - Mouse coatomer protein complex, subunit beta 1 (Copb1) |
CNY 9,030.00 |
