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Copb1 (NM_033370) Mouse Tagged ORF Clone
CAT＃: MG211294
- TrueORF®
Copb1 (tGFP-tagged) - Mouse coatomer protein complex, subunit beta 1 (Copb1)
ORF Plasmid: DDK
"NM_033370" in other vectors (4)
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CNY 7,890.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2610019B04Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211294 representing NM_033370
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGCAGCCGAGAACGTGTGCTATACGTTAATTAACGTGCCAATGGATTCAGAACCCCCTTCGGAAA TCAGCTTAAAAAATGACCTGGAGAAAGGCGATGTGAAGTCAAAGACTGAAGCTTTGAAGAAAGTGATCAT TATGATTCTGAATGGGGAAAAGCTTCCCGGACTCCTGATGACAATCATTCGCTTCGTGCTGCCTCTTCAG GATCACACCATCAAAAAGTTGCTCCTGGTCTTCTGGGAGATTGTTCCTAAAACAACTCCAGATGGAAGGC TCTTACATGAAATGATTCTTGTGTGTGATGCATACAGAAAGGATCTCCAGCACCCCAATGAGTTTATTCG TGGATCTACTCTTCGTTTCCTTTGCAAATTGAAGGAAGCAGAATTGCTGGAACCTTTAATGCCTGCTATC CGTGCGTGCTTGGAACATCGTCACAGCTATGTTAGGAGAAATGCTGTTTTGGCCATCTACACCATCTACA GAAATTTTGAACATCTTATACCTGATGCTCCTGAACTGATACATGATTTTCTTGTGAATGAGAAGGATGC AAGCTGCAAAAGAAATGCATTTATGATGCTAATTCACGCAGATCAGGATCGAGCTTTGGATTATTTAAGT ACATGTATTGATCAAGTTCAGACATTTGGAGACATTCTACAGTTGGTTATTGTTGAACTAATTTATAAGG TCTGTCATGCTAATCCATCAGAAAGGGCCCGCTTCATTCGCTGTATCTATAACTTACTGCAGTCATCTAG TCCTGCTGTAAAATATGAGGCTGCTGGGACACTGGTAACACTGTCAAGTGCACCAACTGCAATAAAGGCT GCTGCTCAGTGTTACATTGATTTAATTATTAAGGAGAGTGATAACAATGTAAAACTCATCGTCCTGGACC GCTTGGTGGAATTAAAAGAGCATCCTGCTCATGAACGAGTCCTACAGGATCTGGTCATGGACATCCTAAG AGTACTGAGTACGCCAGACTTAGAAGTGCGTAAGAAAACGCTGCAGTTAGCACTGGATCTTGTCTCATCT AGGAATGTTGAAGAGTTGGTTATTGTCTTAAAGAAGGAAGTAATTAAAACAAATAACGTGTCTGAGCATG AAGACACTGACAAATACCGACAGCTTCTCGTGCGAACACTGCATTCCTGCTCTGTCCGATTTCCAGATAT GGCTGCAAATGTTATTCCTGTGTTAATGGAATTTCTCAGTGACAGTAATGAAGCAGCAGCTGCTGATGTC TTGGAGTTTGTTCGTGAAGCCATTCAGCGCTTTGACAACCTGAGAATGCTGATTGTTGAGAAGATGCTGG AAGTCTTCCATGCTATTAAGTCTGTCAAGATTTACCGAGGAGCATTGTGGATCCTGGGAGAGTACTGCAG TACAAAGGAAGACATTCAGAGTGTGATGACTGAGGTCCGCAGGTCCCTTGGGGAGATACCAATTGTAGAA TCAGAAATAAAGAAAGAAGCTGGCGAGTTAAAACCAGAAGAAGAAATAACTGTTGGGCCCGTTCAGAAAT TGGTTACGGAGATGGGCACCTATGCTACTCAGAGTGCGCTCAGCAGTTCCAGACCTACCAAGAAAGAAGA AGACAGACCACCCTTGAGAGGGTTCCTCCTGGATGGAGATTTCTTTGTTGCTGCTTCCCTCGCCACAACT CTAACCAAGATTGCATTGCGCTATGTAGCGTTGGTTCAGGAGAAGAAAAAGCAAAATTCTTTTGTTGCTG AGGCTATGTTGCTTATGGCAACTATCCTTCATTTGGGAAAATCCTCTCTTCCTAAGAAGCCAATTACTGA TGATGATGTAGATCGAATTTCCCTGTGCCTCAAGGTCTTATCTGAATGCTCACCTTTAATGAATGACATC TTTAATAAGGAGTGCAGACAGTCTCTTTCCCAAATGTTATCTGCCAAACTCGAAGAAGAGAAACTATCCC AGAAGAAAGAATCTGAAAAGAGAAATGTGACAGTACAGCCTGATGACCCCATTTCCTTCATGCAACTAAC TGCGAAGAATGAAATGAACTGCAAGGAAGATCAGTTCCAGCTGAGTTTGTTGGCAGCAATGGGTAATACT CAGAGGAAAGAGGCAGCTGATCCCCTAGCATCCAAACTTAACAAGGTCACTCAGTTGACAGGCTTCTCGG ATCCAGTGTATGCAGAAGCCTATGTTCATGTCAATCAGTACGACATCGTCCTGGATGTTCTGGTTGTTAA CCAAACCAGTGATACTTTGCAGAACTGCACATTAGAGTTAGCCACTCTAGGGGATCTGAAACTTGTGGAA AAACCATCTCCTTTGACTCTTGCTCCTCATGACTTTGCAAATATTAAAGCTAATGTCAAGGTAGCATCAA CAGAAAACGGAATAATTTTTGGCAATATAGTTTATGATGTTTCTGGAGCAGCAAGTGACAGAAACTGTGT GGTCCTCAGTGACATCCACATTGACATCATGGACTATATCCAGCCTGCAACTTGCACTGATGCTGAGTTC CGTCAGATGTGGGCCGAATTTGAATGGGAAAATAAAGTGACAGTTAACACCAACATGACTGACCTGAATG ACTACTTACAGCACATCCTCAAGTCGACCAACATGAAGTGCCTGACTCCAGAGAAGGCCCTTTCTGGCTA CTGTGGCTTTATGGCAGCCAATCTTTATGCACGTTCTATATTTGGAGAGGATGCGCTTGCAAATGTCAGC ATTGAGAAGCCAGTTCACCAGGGACCAGATGCTGCCGTTACTGGCCATATAAGAATTCGTGCAAAAAGTC AGGGAATGGCCTTGAGCCTTGGAGATAAAATCAACCTGTCTCAAAAGAAGACTAGTCTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211294 representing NM_033370
Red=Cloning site Green=Tags(s) MTAAENVCYTLINVPMDSEPPSEISLKNDLEKGDVKSKTEALKKVIIMILNGEKLPGLLMTIIRFVLPLQ DHTIKKLLLVFWEIVPKTTPDGRLLHEMILVCDAYRKDLQHPNEFIRGSTLRFLCKLKEAELLEPLMPAI RACLEHRHSYVRRNAVLAIYTIYRNFEHLIPDAPELIHDFLVNEKDASCKRNAFMMLIHADQDRALDYLS TCIDQVQTFGDILQLVIVELIYKVCHANPSERARFIRCIYNLLQSSSPAVKYEAAGTLVTLSSAPTAIKA AAQCYIDLIIKESDNNVKLIVLDRLVELKEHPAHERVLQDLVMDILRVLSTPDLEVRKKTLQLALDLVSS RNVEELVIVLKKEVIKTNNVSEHEDTDKYRQLLVRTLHSCSVRFPDMAANVIPVLMEFLSDSNEAAAADV LEFVREAIQRFDNLRMLIVEKMLEVFHAIKSVKIYRGALWILGEYCSTKEDIQSVMTEVRRSLGEIPIVE SEIKKEAGELKPEEEITVGPVQKLVTEMGTYATQSALSSSRPTKKEEDRPPLRGFLLDGDFFVAASLATT LTKIALRYVALVQEKKKQNSFVAEAMLLMATILHLGKSSLPKKPITDDDVDRISLCLKVLSECSPLMNDI FNKECRQSLSQMLSAKLEEEKLSQKKESEKRNVTVQPDDPISFMQLTAKNEMNCKEDQFQLSLLAAMGNT QRKEAADPLASKLNKVTQLTGFSDPVYAEAYVHVNQYDIVLDVLVVNQTSDTLQNCTLELATLGDLKLVE KPSPLTLAPHDFANIKANVKVASTENGIIFGNIVYDVSGAASDRNCVVLSDIHIDIMDYIQPATCTDAEF RQMWAEFEWENKVTVNTNMTDLNDYLQHILKSTNMKCLTPEKALSGYCGFMAANLYARSIFGEDALANVS IEKPVHQGPDAAVTGHIRIRAKSQGMALSLGDKINLSQKKTSL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_033370 |
| ORF Size | 2859 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_033370.2 |
| RefSeq Size | 3315 bp |
| RefSeq ORF | 2862 bp |
| Locus ID | 70349 |
| UniProt ID | Q9JIF7 |
| Gene Summary | The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Involved in the Golgi disassembly and reassembly processes during cell cycle. Involved in autophagy by playing a role in early endosome function. Plays a role in organellar compartmentalization of secretory compartments including endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC), Golgi, trans-Golgi network (TGN) and recycling endosomes, and in biosynthetic transport of CAV1 (By similarity). Plays a functional role in facilitating the transport of kappa-type opioid receptor mRNAs into axons and enhances translation of these proteins in cortical neurons. Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte triglyceride lipase (PNPLA2) with the lipid droplet surface to mediate lipolysis.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222788 | Copb1 (untagged) - Mouse coatomer protein complex, subunit beta 1 (Copb1), (10ug) |
CNY 12,160.00 |
|
| MR211294 | Copb1 (Myc-DDK-tagged) - Mouse coatomer protein complex, subunit beta 1 (Copb1) |
CNY 6,976.00 |
|
| MR211294L3 | Lenti ORF clone of Copb1 (Myc-DDK-tagged) - Mouse coatomer protein complex, subunit beta 1 (Copb1) |
CNY 9,030.00 |
|
| MR211294L4 | Lenti ORF clone of Copb1 (mGFP-tagged) - Mouse coatomer protein complex, subunit beta 1 (Copb1) |
CNY 9,030.00 |
