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Il6st (NM_010560) Mouse Untagged Clone
CAT＃: MC222579
Il6st (untagged) - Mouse interleukin 6 signal transducer (Il6st), (10ug)
CN¥ 10,080.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 5133400A03Rik; AA389424; BB405851; CD130; D13Ertd699e; gp130 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222579 representing NM_010560
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCAGCACCAAGGATTTGGCTAGCGCAAGCTTTGCTTTTTTTCCTCACCACTGAATCTATAGGTCAAC TTTTGGAACCGTGTGGTTACATCTACCCTGAATTTCCAGTTGTCCAGCGCGGCTCGAACTTCACTGCCAT TTGTGTGCTGAAGGAGGCGTGTCTGCAGCATTACTACGTGAATGCCAGCTACATCGTGTGGAAGACCAAC CATGCTGCTGTTCCCAGGGAGCAGGTCACTGTCATCAACAGAACCACGTCCAGTGTCACGTTCACAGACG TGGTCCTCCCGAGCGTGCAGCTCACCTGCAACATCCTGTCCTTTGGGCAGATCGAGCAGAATGTGTATGG AGTCACCATGCTTTCAGGCTTTCCTCCAGATAAACCTACAAATTTGACTTGCATTGTGAATGAGGGGAAG AATATGCTGTGCCAGTGGGACCCCGGAAGGGAGACTTACCTTGAAACAAACTACACTTTGAAATCAGAGT GGGCAACAGAGAAGTTTCCTGATTGCCAGTCAAAGCATGGCACTTCATGTATGGTCAGCTACATGCCCAC CTATTATGTCAACATTGAAGTCTGGGTGGAAGCAGAGAATGCCCTTGGGAAGGTCTCCTCAGAGTCTATC AATTTTGACCCCGTGGATAAAGTGAAACCCACCCCACCATATAATTTATCAGTGACCAACTCAGAAGAAT TATCCAGTATATTAAAGCTATCATGGGTCAGTTCAGGGCTGGGCGGTCTTTTAGATCTAAAGTCTGACAT CCAATATAGGACCAAAGATGCCTCAACTTGGATCCAGGTCCCTCTTGAAGATACAATGTCTCCTCGAACT TCCTTCACTGTGCAGGACCTCAAGCCTTTTACAGAATATGTGTTTAGGATCCGGTCCATTAAGGACAGTG GGAAGGGCTACTGGAGTGACTGGAGTGAGGAGGCTAGTGGGACCACATACGAAGACAGACCATCCAGACC ACCAAGTTTCTGGTATAAGACAAATCCATCCCATGGGCAGGAATATAGATCTGTACGGCTCATATGGAAG GCACTGCCTCTTTCTGAAGCCAATGGGAAAATCTTGGATTATGAAGTGATTCTTACGCAGTCAAAGTCCG TCTCACAAACGTACACAGTCACTGGCACAGAGCTGACCGTGAATCTCACCAATGACCGCTATGTCGCGTC TCTAGCAGCAAGAAACAAGGTGGGCAAATCAGCTGCAGCTGTCCTCACCATCCCCAGCCCCCACGTCACA GCTGCTTATTCTGTAGTGAATCTTAAAGCATTTCCAAAAGATAACCTGCTCTGGGTGGAATGGACACCTC CACCTAAACCCGTGAGCAAGTACATCTTAGAGTGGTGTGTGTTGTCAGAGAACGCACCCTGTGTTGAAGA CTGGCAGCAGGAAGACGCTACCGTGAATCGGACCCACTTGAGAGGACGCCTCCTGGAGAGCAAGTGCTAT CAAATCACAGTAACTCCCGTATTCGCCACGGGGCCCGGAGGCTCTGAGTCCTTGAAGGCGTACCTCAAAC AAGCCGCTCCTGCCAGAGGACCGACTGTTCGGACAAAGAAAGTGGGGAAAAATGAAGCTGTCTTAGCGTG GGACCAGATTCCTGTGGACGACCAGAATGGCTTCATTAGAAACTACTCCATATCTTACAGAACCAGCGTG GGAAAGGAGATGGTTGTGCATGTGGATTCTTCTCACACGGAGTACACGCTGTCCTCTCTGAGTAGTGATA CGTTGTACATGGTCCGAATGGCCGCGTACACAGATGAAGGTGGGAAAGATGGGCCGGAATTCACTTTTAC AACACCAAAGTTCGCTCAAGGAGAAATAGAAGCCATAGTCGTGCCTGTGTGCTTAGCCTTCCTCCTGACA ACCCTGCTGGGCGTCTTGTTCTGCTTTAACAAACGAGATCTAATTAAAAAACACATCTGGCCTAATGTTC CTGATCCTTCCAAGAGTCATATTGCCCAGTGGTCACCTCACACCCCCCCAAGGCACAATTTTAACTCCAA AGATCAAATGTACTCGGACGGCAATTTCACTGATGTAAGCGTTGTGGAAATAGAAGCAAACAACAAGAAG CCTTGTCCAGATGACCTGAAGTCCGTGGACCTGTTCAAGAAGGAGAAAGTGAGTACAGAAGGGCACAGCA GTGGCATCGGGGGCTCTTCATGCATGTCCTCCTCCAGGCCCAGCATCTCCAGCAACGAGGAGAATGAGTC TGCTCAGAGCACCGCCAGCACGGTGCAGTACTCCACTGTGGTGCACAGCGGCTACAGGCACCAGGTCCCG TCCGTGCAAGTGTTCTCAAGGTCCGAGTCCACCCAGCCCCTGCTAGACTCGGAGGAGCGGCCAGAAGACC TGCAGCTGGTGGATAGTGTAGACGGTGGGGATGAGATCTTGCCCAGGCAATCGTATTTCAAGCAGAACTG CAGTCAGCCTGAAGCCTGTCCAGAGATTTCACATTTTGAAAGGTCAAACCAGGTTTTGTCCGGCAATGAG GAGGATTTTGTCAGACTGAAGCAGCAGCAGGTTTCAGATCACATTTCTCAGCCCTATGGATCCGAGCAAC GGAGGCTGTTTCAGGAAGGCTCTACAGCGGATGCTCTTGGCACGGGGGCTGATGGACAGATGGAGAGATT TGAATCTGTTGGAATGGAGACCACAATTGATGAAGAAATTCCCAAAAGTTACTTGCCACAGACTGTAAGA CAAGGTGGCTACATGCCGCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_010560 |
| Insert Size | 2754 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC058679, AAH58679 |
| RefSeq Size | 5452 bp |
| RefSeq ORF | 2754 bp |
| Locus ID | 16195 |
| UniProt ID | Q00560 |
| Gene Summary | Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize IL6ST for initiating signal transmission. Binding of IL6 to IL6R induces IL6ST homodimerization and formation of a high-affinity receptor complex, which activates Janus kinases (PubMed:1602143). That causes phosphorylation of IL6ST tyrosine residues which in turn activates STAT3 (PubMed:10661409). Mediates signals which regulate immune response, hematopoiesis, pain control and bone metabolism (PubMed:10661409, PubMed:26255596, PubMed:25057188, PubMed:8552649). Has a role in embryonic development (PubMed:10661409). Does not bind IL6 (By similarity). Essential for survival of motor and sensory neurons and for differentiation of astrocytes (PubMed:10377352). Required for expression of TRPA1 in nociceptive neurons (PubMed:25057188). Required for the maintenance of PTH1R expression in the osteoblast lineage and for the stimulation of PTH-induced osteoblast differentiation (PubMed:25228504). Required for normal trabecular bone mass and cortical bone composition (PubMed:24339143, PubMed:9348227, PubMed:26255596).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226651 | Il6st (tGFP-tagged) - Mouse interleukin 6 signal transducer (Il6st), (10ug) |
CN¥ 11,672.00 |
|
| MR226651 | Il6st (Myc-DDK-tagged) - Mouse interleukin 6 signal transducer (Il6st) |
CN¥ 10,072.00 |
|
| MR226651L3 | Lenti ORF clone of Il6st (Myc-DDK-tagged) - Mouse interleukin 6 signal transducer (Il6st) |
CN¥ 11,020.00 |
|
| MR226651L4 | Lenti ORF clone of Il6st (mGFP-tagged) - Mouse interleukin 6 signal transducer (Il6st) |
CN¥ 12,472.00 |
