Irag1 (NM_194464) Mouse Untagged Clone

CAT#: MC222145

Mrvi1 (untagged) - Mouse MRV integration site 1 (Mrvi1), transcript variant 2, (10ug)



  "NM_194464" in other vectors (4)

CNY 10,930.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms BB115629; Irag; Mrvi1; R; Ris1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC222145 representing NM_194464
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCCCACATTCCTGAGGATGAGGAGCCCCCCGGAGAGCCACAGGCAGCCCAGACCCAAGACTCTCCCT
CTGCAGGACCATTTCCCAGCCCTCCCACAATCGTCCTGACGGGGGATGCCAGTTCACCTGAAGGAGAGAC
TGACAAAAACCTAGTCAACAGGGCTCCCAGCCCTCACCGGAGGCTTTCCCACCGACACCTGAAGGTCTCC
ACTGCTTCGCTGACTTCTGTGGACCCCTCGGGGCATGTCATTGACCTGGTGAATGACCAGCTGCCGGACA
TCAGCATCTCAGAAGAGGACAAGAAAAAAAACCTGGCACTTCTGGAAGAAGCCAAGTTGGTGAGTGAGCG
GTTCCTGACCCGCCGTGGGAGGAAATCAAGGAGCAGCCTCGGAGACTCCCCATCAGCTGTTTCTCCAAAC
CTCAGCTCTGGGGCTTCTCCTGCATCCTCCAGGAGCTGCTCCCTCACCATCTCCACATCACCAGGTTTGG
ACATATGCAGTGGCCCTCAGTCCCCTCTGCCAGGAGCACCACCACAGCAAAAGGGACATGAGGACGGGGT
CTCCTCGCCTTGCCCCGGAGAGCCTAATGTCTCCAAAGGGCTAGCTGACCTGAAGCAGAATGACCAGAGA
AAAGTGTCTCAAGGCAGACTGGCTCCTCGCTCTCCCACAGTGGAGAAAACCAAGGAACTTACAGTAGAAC
AAAAGGAAAACTTTGATCCCCTTCAGCACGTGGAGGCCACGCCCATGGCTCAGGCTTCTGGAGCAAGCAT
CAGTGGGAAAATGGCTCTGAACAGCCCCCAGCCTGGCCCTGCTGAGATGGAGCTGGGGAGGCAGCTCCTG
AAGACAGCCCGGGAAGGCAACCCTCTGCCCAGAACCACAGCCCAGGGCTCAGGAGGAACGGTCTCCCCAC
ATTCCCTGGGGCAGGGCTCTGCTGGAGAGCCTATGGGACCCAAGGCTGGCTCCAAGGCTGAGCTGAGGTC
CCCTGTGTCCCGGCCCCCCCTGATTCGGGGGGTCTCCTGGGACAGCAGCCCTGAAGAACCCGGTCCCCTG
CTGCAGAAGGTGCTCGCTAAGCTGCCCCTGGCAGAGGAAGAGAAGCGGTTTCCAGGCAAAGCCAAGCCAG
CCAAGCCACCTGGGCTCAAAGACTTCCAGATACAAGTGCAGCCGGTGCGCATGCAGAAGCTGACCAAGCT
CCGGGAGGAACACATCCTGATGAGAAACCAGAACCTGGTGGGGTTCAAGCTCCCTGAACTGAGTGAGGCC
GCGGAGCAGGACAAAGGAGTCTCACCTGAACTCGCCCCAGCAGCTGAGGAAGAAGAGTCCAAGAGTGGTC
TGGATGTGATGCCCAACATTTCTGACATATTGCTGCGCAAACTTAGGGTTCACAAGTCACTCACTGGAAG
CGCCCCTCCACTCACTGAAAAGGAAGTTGAGAACGTGTTTGTACAACTGTCCTTGGCCTTTAGAAATGAC
AGCTACACCTTGGAGTCTAGAATTAACCAGGCTGAAAGGGAGCGCAACCTGACAGAAGAGAACACGGAGA
AGGAACTGGAAAACTTCAAAGCCTCCATAACGTCCTCAGCAAACATCTGGTACCACTGCGAACACCGGGA
GACCTATCAGAAACTTCTGGAAGACATCGCCGTCTTGCATCGTCTGGCTGCCCGCCTCTCCAGTCGGGCT
GAAGTGGTGGGTGCTGTTCGCCAGGAAAAGCGCATGTCAAAGGCCACGGAAGTGATGATGCAGTATGTGG
AGAATCTGAAGAGAACGTATGAGAAGGACCATGCTGAGCTCATGGAATTTAAGAAACTTGCAAATCAGAA
TTCCAGTCGCAGCTGTGGGCCCTCTGAAGACGGGGTTCCTCGAACGGCGCGATCTATGTCCCTCACAATG
GGAAAGAACATGCCCCGCCGGAGGGTCAGCGTTGCTGTGGTTCCCAAGTTTAATGCCCTGAACCTGCCTG
GCCAAGCCCCGAGCTCTTCACCCATGCCTTCCCTACCAGCTCTGTCTGAATCGTCCAATGGGAAGAGCAG
CATCTCCGTCTCCCCAGCGCTGCCTGCACTTTTGGAAAATGGAAAGACAAATGCAGAGGCTAACTGCGAA
GTGGGTGCCCCTGTGCCACTCCCAAGCTGTCTGGAGGAGACGAGCCAGGAGACCAAGGCCAAGGCAGAGG
AAGAAGCCTACAGCAAAGGATACCAGGAAGGGGTAAAGAAGACCGAAGAACTTCAAGACCTGAAGGAGGA
GGAGGAGGAAGAACAGAAGACGGAGAGTCCCGAGGAACCAGAAGAGGTAGAAGAAACCCAGGAGGACGAG
AAGGACCAGGGAAGCAGCAAACTTGAAGAACTGGTCCACTTCCTACAAGTCATGTATCCTAAATTGTGTC
AACACTGGCAAGTGATCTGGATGATGGCCGCAGTGATGCTGGTCTTGAGTGTTGTTCTCGGGCTGTACAG
TTCCTATAACTCCTGCACAGAGGAGGCCGATGGGCCCCCTGGGAGATCCACCTGCTCTGCAGCCCAGCGG
GACTCCTGGTGGAGCTCCGGACTCCAGCAAGAGCTGCCAGCAGAGCAGTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_194464
Insert Size 2571 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_194464.3, NP_919446.2
RefSeq Size 6211 bp
RefSeq ORF 2571 bp
Locus ID 17540
Gene Summary This gene is a putative tumor suppressor gene that is frequently disrupted by mouse AIDS-related virus (MRV). The encoded protein participates in signaling by nitric oxide (NO) to inhibit intracellular calcium release and platelet aggregation in cardiovascular tissue. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2013]
Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) has a shorter N-terminus compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no quality transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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