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Irag1 (NM_194464) Mouse Tagged ORF Clone
CAT＃: MR221913
- TrueORF®
Mrvi1 (Myc-DDK-tagged) - Mouse MRV integration site 1 (Mrvi1), transcript variant 2
ORF Plasmid: tGFP
"NM_194464" in other vectors (4)
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CNY 6,272.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BB115629; Irag; Mrvi1; R; Ris1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR221913 representing NM_194464
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCACATTCCTGAGGATGAGGAGCCCCCCGGAGAGCCACAGGCAGCCCAGACCCAAGACTCTCCCT CTGCAGGACCATTTCCCAGCCCTCCCACAATCGTCCTGACGGGGGATGCCAGTTCACCTGAAGGAGAGAC TGACAAAAACCTAGTCAACAGGGCTCCCAGCCCTCACCGGAGGCTTTCCCACCGACACCTGAAGGTCTCC ACTGCTTCGCTGACTTCTGTGGACCCCTCGGGGCATGTCATTGACCTGGTGAATGACCAGCTGCCGGACA TCAGCATCTCAGAAGAGGACAAGAAAAAAAACCTGGCACTTCTGGAAGAAGCCAAGTTGGTGAGTGAGCG GTTCCTGACCCGCCGTGGGAGGAAATCAAGGAGCAGCCTCGGAGACTCCCCATCAGCTGTTTCTCCAAAC CTCAGCTCTGGGGCTTCTCCTGCATCCTCCAGGAGCTGCTCCCTCACCATCTCCACATCACCAGGTTTGG ACATATGCAGTGGCCCTCAGTCCCCTCTGCCAGGAGCACCACCACAGCAAAAGGGACATGAGGACGGGGT CTCCTCGCCTTGCCCCGGAGAGCCTAATGTCTCCAAAGGGCTAGCTGACCTGAAGCAGAATGACCAGAGA AAAGTGTCTCAAGGCAGACTGGCTCCTCGCTCTCCCACAGTGGAGAAAACCAAGGAACTTACAGTAGAAC AAAAGGAAAACTTTGATCCCCTTCAGCACGTGGAGGCCACGCCCATGGCTCAGGCTTCTGGAGCAAGCAT CAGTGGGAAAATGGCTCTGAACAGCCCCCAGCCTGGCCCTGCTGAGATGGAGCTGGGGAGGCAGCTCCTG AAGACAGCCCGGGAAGGCAACCCTCTGCCCAGAACCACAGCCCAGGGCTCAGGAGGAACGGTCTCCCCAC ATTCCCTGGGGCAGGGCTCTGCTGGAGAGCCTATGGGACCCAAGGCTGGCTCCAAGGCTGAGCTGAGGTC CCCTGTGTCCCGGCCCCCCCTGATTCGGGGGGTCTCCTGGGACAGCAGCCCTGAAGAACCCGGTCCCCTG CTGCAGAAGGTGCTCGCTAAGCTGCCCCTGGCAGAGGAAGAGAAGCGGTTTCCAGGCAAAGCCAAGCCAG CCAAGCCACCTGGGCTCAAAGACTTCCAGATACAAGTGCAGCCGGTGCGCATGCAGAAGCTGACCAAGCT CCGGGAGGAACACATCCTGATGAGAAACCAGAACCTGGTGGGGTTCAAGCTCCCTGAACTGAGTGAGGCC GCGGAGCAGGACAAAGGAGTCTCACCTGAACTCGCCCCAGCAGCTGAGGAAGAAGAGTCCAAGAGTGGTC TGGATGTGATGCCCAACATTTCTGACATATTGCTGCGCAAACTTAGGGTTCACAAGTCACTCACTGGAAG CGCCCCTCCACTCACTGAAAAGGAAGTTGAGAACGTGTTTGTACAACTGTCCTTGGCCTTTAGAAATGAC AGCTACACCTTGGAGTCTAGAATTAACCAGGCTGAAAGGGAGCGCAACCTGACAGAAGAGAACACGGAGA AGGAACTGGAAAACTTCAAAGCCTCCATAACGTCCTCAGCAAACATCTGGTACCACTGCGAACACCGGGA GACCTATCAGAAACTTCTGGAAGACATCGCCGTCTTGCATCGTCTGGCTGCCCGCCTCTCCAGTCGGGCT GAAGTGGTGGGTGCTGTTCGCCAGGAAAAGCGCATGTCAAAGGCCACGGAAGTGATGATGCAGTATGTGG AGAATCTGAAGAGAACGTATGAGAAGGACCATGCTGAGCTCATGGAATTTAAGAAACTTGCAAATCAGAA TTCCAGTCGCAGCTGTGGGCCCTCTGAAGACGGGGTTCCTCGAACGGCGCGATCTATGTCCCTCACAATG GGAAAGAACATGCCCCGCCGGAGGGTCAGCGTTGCTGTGGTTCCCAAGTTTAATGCCCTGAACCTGCCTG GCCAAGCCCCGAGCTCTTCACCCATGCCTTCCCTACCAGCTCTGTCTGAATCGTCCAATGGGAAGAGCAG CATCTCCGTCTCCCCAGCGCTGCCTGCACTTTTGGAAAATGGAAAGACAAATGCAGAGGCTAACTGCGAA GTGGGTGCCCCTGTGCCACTCCCAAGCTGTCTGGAGGAGACGAGCCAGGAGACCAAGGCCAAGGCAGAGG AAGAAGCCTACAGCAAAGGATACCAGGAAGGGGTAAAGAAGACCGAAGAACTTCAAGACCTGAAGGAGGA GGAGGAGGAAGAACAGAAGACGGAGAGTCCCGAGGAACCAGAAGAGGTAGAAGAAACCCAGGAGGACGAG AAGGACCAGGGAAGCAGCAAACTTGAAGAACTGGTCCACTTCCTACAAGTCATGTATCCTAAATTGTGTC AACACTGGCAAGTGATCTGGATGATGGCCGCAGTGATGCTGGTCTTGAGTGTTGTTCTCGGGCTGTACAG TTCCTATAACTCCTGCACAGAGGAGGCCGATGGGCCCCCTGGGAGATCCACCTGCTCTGCAGCCCAGCGG GACTCCTGGTGGAGCTCCGGACTCCAGCAAGAGCTGCCAGCAGAGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR221913 representing NM_194464
Red=Cloning site Green=Tags(s) MPHIPEDEEPPGEPQAAQTQDSPSAGPFPSPPTIVLTGDASSPEGETDKNLVNRAPSPHRRLSHRHLKVS TASLTSVDPSGHVIDLVNDQLPDISISEEDKKKNLALLEEAKLVSERFLTRRGRKSRSSLGDSPSAVSPN LSSGASPASSRSCSLTISTSPGLDICSGPQSPLPGAPPQQKGHEDGVSSPCPGEPNVSKGLADLKQNDQR KVSQGRLAPRSPTVEKTKELTVEQKENFDPLQHVEATPMAQASGASISGKMALNSPQPGPAEMELGRQLL KTAREGNPLPRTTAQGSGGTVSPHSLGQGSAGEPMGPKAGSKAELRSPVSRPPLIRGVSWDSSPEEPGPL LQKVLAKLPLAEEEKRFPGKAKPAKPPGLKDFQIQVQPVRMQKLTKLREEHILMRNQNLVGFKLPELSEA AEQDKGVSPELAPAAEEEESKSGLDVMPNISDILLRKLRVHKSLTGSAPPLTEKEVENVFVQLSLAFRND SYTLESRINQAERERNLTEENTEKELENFKASITSSANIWYHCEHRETYQKLLEDIAVLHRLAARLSSRA EVVGAVRQEKRMSKATEVMMQYVENLKRTYEKDHAELMEFKKLANQNSSRSCGPSEDGVPRTARSMSLTM GKNMPRRRVSVAVVPKFNALNLPGQAPSSSPMPSLPALSESSNGKSSISVSPALPALLENGKTNAEANCE VGAPVPLPSCLEETSQETKAKAEEEAYSKGYQEGVKKTEELQDLKEEEEEEQKTESPEEPEEVEETQEDE KDQGSSKLEELVHFLQVMYPKLCQHWQVIWMMAAVMLVLSVVLGLYSSYNSCTEEADGPPGRSTCSAAQR DSWWSSGLQQELPAEQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_194464 |
| ORF Size | 2568 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_194464.3, NP_919446.2 |
| RefSeq Size | 5835 bp |
| RefSeq ORF | 2571 bp |
| Locus ID | 17540 |
| MW | 93.6 kDa |
| Gene Summary | This gene is a putative tumor suppressor gene that is frequently disrupted by mouse AIDS-related virus (MRV). The encoded protein participates in signaling by nitric oxide (NO) to inhibit intracellular calcium release and platelet aggregation in cardiovascular tissue. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222145 | Mrvi1 (untagged) - Mouse MRV integration site 1 (Mrvi1), transcript variant 2, (10ug) |
CNY 10,930.00 |
|
| MG221913 | Mrvi1 (tGFP-tagged) - Mouse MRV integration site 1 (Mrvi1) transcript variant 2, (10ug) |
CNY 7,890.00 |
|
| MR221913L3 | Lenti ORF clone of Mrvi1 (Myc-DDK-tagged) - Mouse MRV integration site 1 (Mrvi1), transcript variant 2 |
CNY 9,030.00 |
|
| MR221913L4 | Lenti ORF clone of Mrvi1 (mGFP-tagged) - Mouse MRV integration site 1 (Mrvi1), transcript variant 2 |
CNY 9,030.00 |
