L3mbtl1 (NM_001081338) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	C630004G01; L3mbtl; mKIAA0681
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC221900 representing NM_001081338 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGGCACACTGACATGGAGATACTAAGGACAGTGAAAGGGTCTTCCACAGGGGAGGTCAACGTGC ACCTGGTGGCCAGAGACAGCGCAGGTCCCCACCCTCAGCTGCCTACAACCGCCTTCATTATTCCTACCAA TGCAGCTACGCTTGGTCTGCCTAGCACTGCCCTGGATGTGCCCTATCCCCGGGAGCCAGTCCACGTGGGT GCCCTGGAGCGGGTGGCTGGCAGCGAACCCGTAACAGCTACTATTCTGCCACAGCTAAGCACTGGAACGG GGACCAATAGCACAGTCAGGCTTCTGGATTGGACTGGCGTTTCTGCACCTTTACCTGGTAGCGGCATGCG GTTCCGGATAAATGAGTACGCGCCACTGAACATGATAGGAGTGGAGCGACCACGAAGCCCGGAGCAGCGA CATGAGGGTGGCATGGCCAGGAGAGATGCGGGCATCCAGCACCCTGATGTTCACCAGGACCGTCAAGATA TAACTTCTCTGGAACCCCCGGTAGATGCGAGTTCCTGCAAGTGCCAGGCTTGTGGGCCTCAACAAAGTAG TGGCCTAGACGTTGGTTCATCGGGAGACCGATGCTCTCAGCCTTTCCAGAAGCGGTCAGTCATAGTAGAG AACTCAGGCTGTACCATCGCTTCTGAGCTCCTGAAACCCATGAAAAAGAGGAAACATAAGGAGTATCAGA GCCCATCAGAGGAGTCAGAGCCGGAGGCTGTGAAGCAAGGAGAAGGAAAAGACGCAGAGCGAGAGCCCAC CCCTAGCACCCCGGAGAATGAAGAGTGGAGCAGAAGCCAGCTGGTGTCCAGTGAGAAGAAGGACGGCTGG TCCTGGGAGTCCTACCTGGAGGAGCAGAAGGCCGTCACAGCCCCAGTTAGCCTCTTCCAGGACTCCCAGG CAGTCACCCATAACAAGAATGGCTTCAAACTGGGCATGAAGCTGGAAGGCATTGACCCCCAACACCCGTC AATGTACTTCATCCTCACTGTGGCTGAGGTGTGTGGCTACCGCCTGCGCCTGCACTTTGATGGCTACTCT GAGTGCCACGACTTCTGGGTCAATGCCAACTCCCCTGATATTCATCCCGCTGGCTGGTTTGAGAAGACTG GACACAAGCTGCAGCTTCCCAAAGGTTACAAGGAAGAGGAGTTCAGCTGGAGCCAGTACCTGCGCAGCAC GAAAGCTCAGGCAGCCCCCAAGCACCTGTTTGTAAGCCAGAGCCACAGTACTCCACCTGTGGGCTTCCAG GTGGGCATGAAGCTGGAAGCTGTGGACCGCATGAACCCTTCCCTCGTGTGTGTGGCCAGTGTGACCGATG TGGTGGACAGTCGCTTCCTGGTGCACTTTGATGACTGGGGCGATACTTACGACTACTGGTGTGATCCCAG CAGCCCCTACATCCACCCAGTAGGCTGGTGCCAGAAGCAAGGAAAGCCGCTCACCCCTCCACAAGACTAC CCAGACCCTGACAGCTTCTGCTGGGAGAAATATCTAGAAGAAACTGGGACCTCGGCTGTGCCCAATTGGG CCTTCAAAGTGCGACCCCCTCACAGCTTCCTGGTGAACATGAAGCTGGAGGCTGTAGACCGCCGGAACCC AGCACTGATCCGGGTAGCCAGTGTGGAAGATGTGGAGGATCATCGGATAAAGCTCCACTTTGATGGCTGG AGTCACAATTATGATTTCTGGATTGATGCCGATCACCCAGACATCCACCCTGCAGGCTGGTGCTCCAAGA CAGGACATCCCCTGGAGCCTCCTCTCCGGCCCAGAGAGTCCAGCTCTGTCTCCCCTGGGGGATGCCCCCC TCTCAGCCACAGGAGCCCACCCCACACAAAGACCTCCAAATATAACTTTCACCACCGAAAGTGCCCCACT CCTGGCTGTGACGGCTCCGGCCATGTCACTGGCAAGTTCACAGCTCACCATTGCCTCTCTGGCTGCCCGC TGGCCGAGAAAAACCAGAGCCGGCTGAAAGCGGAACTGTCCGACTCGGAGACCGCAGCCAGGAAGAAGAA CCCGTCTAATTTGTCCCCAAGGAAAAAGCCTCGCCATCAAGGCCGGATTGGACGCCCTCCAAAGTATCGC AAGATTCCAGAAGAAGATCTCCAAGCCCTCCCTCCCAGTGTGGTACACCAGTCCCTCTTCATGTCCACCT TGCCGACCCACGCTGATCGCCCACTCTCTGTGTGCTGGGAGCAACACTGCAAGCTGTTGCCAGGAGTGGC GGGCATCTCAGCCTCCACAGTCTCTAAGTGGACCATTGAAGAGGTCTTTGGCTTCGTTCAGACCTTGACG GGCTCTGAGGACCAAGCGCGCCTCTTCAAAGACGAGATGATTGACGGCGAGGCCTTCCTTTTGCTGACAC AGGCGGACATTGTGAAGATCATGAGTGTCAAGCTAGGCCCAGCCTTGAAGATCTATAACGCCATTCTCAT GTTCAAAAACACTGACGATGCCTTTAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001081338
Insert Size	2481 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001081338.1, NP_001074807.1
RefSeq Size	2733 bp
RefSeq ORF	2481 bp
Locus ID	241764
UniProt ID	A2A5N8
Gene Summary	Polycomb group (PcG) protein that specifically recognizes and binds mono- and dimethyllysine residues on target proteins, therey acting as a 'reader' of a network of post-translational modifications. PcG proteins maintain the transcriptionally repressive state of genes: acts as a chromatin compaction factor by recognizing and binding mono- and dimethylated histone H1b/HIST1H1E at 'Lys-26' (H1bK26me1 and H1bK26me2) and histone H4 at 'Lys-20' (H4K20me1 and H4K20me2), leading to condense chromatin and repress transcription. Recognizes and binds p53/TP53 monomethylated at 'Lys-382', leading to repress p53/TP53-target genes. Also recognizes and binds RB1/RB monomethylated at 'Lys-860'. Participates in the ETV6-mediated repression. Probably plays a role in cell proliferation. Overexpression induces multinucleated cells, suggesting that it is required to accomplish normal mitosis (By similarity).[UniProtKB/Swiss-Prot Function]

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