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L3mbtl1 (NM_001081338) Mouse Tagged ORF Clone
CAT＃: MG221523
- TrueORF®
L3mbtl1 (tGFP-tagged) - Mouse l(3)mbt-like (Drosophila) (L3mbtl), (10ug)
ORF Plasmid: DDK
"NM_001081338" in other vectors (2)
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CNY 6,370.00
货期*
4周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C630004G01; L3mbtl; mKIAA0681 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG221523 representing NM_001081338
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGGCACACTGACATGGAGATACTAAGGACAGTGAAAGGGTCTTCCACAGGGGAGGTCAACGTGC ACCTGGTGGCCAGAGACAGCGCAGGTCCCCACCCTCAGCTGCCTACAACCGCCTTCATTATTCCTACCAA TGCAGCTACGCTTGGTCTGCCTAGCACTGCCCTGGATGTGCCCTATCCCCGGGAGCCAGTCCACGTGGGT GCCCTGGAGCGGGTGGCTGGCAGCGAACCCGTAACAGCTACTATTCTGCCACAGCTAAGCACTGGAACGG GGACCAATAGCACAGTCAGGCTTCTGGATTGGACTGGCGTTTCTGCACCTTTACCTGGTAGCGGCATGCG GTTCCGGATAAATGAGTACGCGCCACTGAACATGATAGGAGTGGAGCGACCACGAAGCCCGGAGCAGCGA CATGAGGGTGGCATGGCCAGGAGAGATGCGGGCATCCAGCACCCTGATGTTCACCAGGACCGTCAAGATA TAACTTCTCTGGAACCCCCGGTAGATGCGAGTTCCTGCAAGTGCCAGGCTTGTGGGCCTCAACAAAGTAG TGGCCTAGACGTTGGTTCATCGGGAGACCGATGCTCTCAGCCTTTCCAGAAGCGGTCAGTCATAGTAGAG AACTCAGGCTGTACCATCGCTTCTGAGCTCCTGAAACCCATGAAAAAGAGGAAACATAAGGAGTATCAGA GCCCATCAGAGGAGTCAGAGCCGGAGGCTGTGAAGCAAGGAGAAGGAAAAGACGCAGAGCGAGAGCCCAC CCCTAGCACCCCGGAGAATGAAGAGTGGAGCAGAAGCCAGCTGGTGTCCAGTGAGAAGAAGGACGGCTGG TCCTGGGAGTCCTACCTGGAGGAGCAGAAGGCCGTCACAGCCCCAGTTAGCCTCTTCCAGGACTCCCAGG CAGTCACCCATAACAAGAATGGCTTCAAACTGGGCATGAAGCTGGAAGGCATTGACCCCCAACACCCGTC AATGTACTTCATCCTCACTGTGGCTGAGGTGTGTGGCTACCGCCTGCGCCTGCACTTTGATGGCTACTCT GAGTGCCACGACTTCTGGGTCAATGCCAACTCCCCTGATATTCATCCCGCTGGCTGGTTTGAGAAGACTG GACACAAGCTGCAGCTTCCCAAAGGTTACAAGGAAGAGGAGTTCAGCTGGAGCCAGTACCTGCGCAGCAC GAAAGCTCAGGCAGCCCCCAAGCACCTGTTTGTAAGCCAGAGCCACAGTACTCCACCTGTGGGCTTCCAG GTGGGCATGAAGCTGGAAGCTGTGGACCGCATGAACCCTTCCCTCGTGTGTGTGGCCAGTGTGACCGATG TGGTGGACAGTCGCTTCCTGGTGCACTTTGATGACTGGGGCGATACTTACGACTACTGGTGTGATCCCAG CAGCCCCTACATCCACCCAGTAGGCTGGTGCCAGAAGCAAGGAAAGCCGCTCACCCCTCCACAAGACTAC CCAGACCCTGACAGCTTCTGCTGGGAGAAATATCTAGAAGAAACTGGGACCTCGGCTGTGCCCAATTGGG CCTTCAAAGTGCGACCCCCTCACAGCTTCCTGGTGAACATGAAGCTGGAGGCTGTAGACCGCCGGAACCC AGCACTGATCCGGGTAGCCAGTGTGGAAGATGTGGAGGATCATCGGATAAAGCTCCACTTTGATGGCTGG AGTCACAATTATGATTTCTGGATTGATGCCGATCACCCAGACATCCACCCTGCAGGCTGGTGCTCCAAGA CAGGACATCCCCTGGAGCCTCCTCTCCGGCCCAGAGAGTCCAGCTCTGTCTCCCCTGGGGGATGCCCCCC TCTCAGCCACAGGAGCCCACCCCACACAAAGACCTCCAAATATAACTTTCACCACCGAAAGTGCCCCACT CCTGGCTGTGACGGCTCCGGCCATGTCACTGGCAAGTTCACAGCTCACCATTGCCTCTCTGGCTGCCCGC TGGCCGAGAAAAACCAGAGCCGGCTGAAAGCGGAACTGTCCGACTCGGAGACCGCAGCCAGGAAGAAGAA CCCGTCTAATTTGTCCCCAAGGAAAAAGCCTCGCCATCAAGGCCGGATTGGACGCCCTCCAAAGTATCGC AAGATTCCAGAAGAAGATCTCCAAGCCCTCCCTCCCAGTGTGGTACACCAGTCCCTCTTCATGTCCACCT TGCCGACCCACGCTGATCGCCCACTCTCTGTGTGCTGGGAGCAACACTGCAAGCTGTTGCCAGGAGTGGC GGGCATCTCAGCCTCCACAGTCTCTAAGTGGACCATTGAAGAGGTCTTTGGCTTCGTTCAGACCTTGACG GGCTCTGAGGACCAAGCGCGCCTCTTCAAAGACGAGATGATTGACGGCGAGGCCTTCCTTTTGCTGACAC AGGCGGACATTGTGAAGATCATGAGTGTCAAGCTAGGCCCAGCCTTGAAGATCTATAACGCCATTCTCAT GTTCAAAAACACTGACGATGCCTTTAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG221523 representing NM_001081338
Red=Cloning site Green=Tags(s) MEGHTDMEILRTVKGSSTGEVNVHLVARDSAGPHPQLPTTAFIIPTNAATLGLPSTALDVPYPREPVHVG ALERVAGSEPVTATILPQLSTGTGTNSTVRLLDWTGVSAPLPGSGMRFRINEYAPLNMIGVERPRSPEQR HEGGMARRDAGIQHPDVHQDRQDITSLEPPVDASSCKCQACGPQQSSGLDVGSSGDRCSQPFQKRSVIVE NSGCTIASELLKPMKKRKHKEYQSPSEESEPEAVKQGEGKDAEREPTPSTPENEEWSRSQLVSSEKKDGW SWESYLEEQKAVTAPVSLFQDSQAVTHNKNGFKLGMKLEGIDPQHPSMYFILTVAEVCGYRLRLHFDGYS ECHDFWVNANSPDIHPAGWFEKTGHKLQLPKGYKEEEFSWSQYLRSTKAQAAPKHLFVSQSHSTPPVGFQ VGMKLEAVDRMNPSLVCVASVTDVVDSRFLVHFDDWGDTYDYWCDPSSPYIHPVGWCQKQGKPLTPPQDY PDPDSFCWEKYLEETGTSAVPNWAFKVRPPHSFLVNMKLEAVDRRNPALIRVASVEDVEDHRIKLHFDGW SHNYDFWIDADHPDIHPAGWCSKTGHPLEPPLRPRESSSVSPGGCPPLSHRSPPHTKTSKYNFHHRKCPT PGCDGSGHVTGKFTAHHCLSGCPLAEKNQSRLKAELSDSETAARKKNPSNLSPRKKPRHQGRIGRPPKYR KIPEEDLQALPPSVVHQSLFMSTLPTHADRPLSVCWEQHCKLLPGVAGISASTVSKWTIEEVFGFVQTLT GSEDQARLFKDEMIDGEAFLLLTQADIVKIMSVKLGPALKIYNAILMFKNTDDAFK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001081338 |
| ORF Size | 2478 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001081338.2 |
| RefSeq Size | 2733 bp |
| RefSeq ORF | 2481 bp |
| Locus ID | 241764 |
| UniProt ID | A2A5N8 |
| Gene Summary | Polycomb group (PcG) protein that specifically recognizes and binds mono- and dimethyllysine residues on target proteins, therey acting as a 'reader' of a network of post-translational modifications. PcG proteins maintain the transcriptionally repressive state of genes: acts as a chromatin compaction factor by recognizing and binding mono- and dimethylated histone H1b/HIST1H1E at 'Lys-26' (H1bK26me1 and H1bK26me2) and histone H4 at 'Lys-20' (H4K20me1 and H4K20me2), leading to condense chromatin and repress transcription. Recognizes and binds p53/TP53 monomethylated at 'Lys-382', leading to repress p53/TP53-target genes. Also recognizes and binds RB1/RB monomethylated at 'Lys-860'. Participates in the ETV6-mediated repression. Probably plays a role in cell proliferation. Overexpression induces multinucleated cells, suggesting that it is required to accomplish normal mitosis (By similarity).[UniProtKB/Swiss-Prot Function] |
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